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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis and cytogenetic characterization of intergeneric hybrids of Diplotaxis siifolia with Brassica rapa and B. juncea (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Intergeneric hybrids involving a wild crucifer, Diplotaxis siifolia (2n = 20; DsDs), and two crop Brassica species, namely Brassica rapa (2n = 20; AA) and B. juncea (2n = 36; AABB), were developed through sequential ovary/ovule culture. Hybridization was successful only when D. siifolia was used as the female parent, indicating unilateral cross incompatibility. Hybrids were intermediate between the parents for morphological characteristics but had low male as well as female fertility. Meiotic studies of hybrids revealed partial homoeology between Ds and A/B genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00805.x
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  • 2
    Electronic Resource
    Electronic Resource
    Erucic acid heredity in Brassica juncea - some additional information (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Genetic studies were undertaken to reassess erucic acid heredity in Brassica juncea. Analysis of segregation in F2 and BC1 generations from two zero × high erucic acid crosses indicated that higher erucic acid in B. juncea was controlled by two dominant genes with additive effects, whereas segregation in a cross involving ‘CCWF 16′, a genotype having intermediate erucic acid (25.6%), and a zero erucic acid strain, indicated monogenic dominant control for intermediate erucic acid content. The B. juncea strain ‘CCWF 16’ was developed by hybridizing high-erucic acid B. juncea cv.‘WF-1’ with a ‘0’ erucic B. rapa cv.‘Candle’ followed by backcrossing with ‘WF-1’ and half-seed selection for low erucic acid in each backcross generation. This strategy resulted in substitution of the high erucic acid allele present in the A genome of B. juncea (AABB) by the zero erucic acid allele associated with ‘A’ genome of ‘Candle’. The intermediate erucic acid content in ‘CCWF 16’ was thus attributed to a gene present in the ‘BB’ genome. Experimental data clearly suggested that the gene (E2) associated with the A genome had a greater contribution to the total erucic acid content in B. juncea than the gene (E1) located on the B genome. This provided experimental evidence for a previous suggestion of unequal contributions of two dominant genes (E1= 12%, E2= 20%) to high erucic acid content in conventional digenomic Brassica species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.731805.x
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  • 3
    Electronic Resource
    Electronic Resource
    Production of an interspecific hybrid between Brassica fruticulosa and B. rapa (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An interspecific hybrid between a wild species, Brassica fruticulosa (2n = 16, FF) and a crop Brassica species, B. rapa (2n = 20, AA) was synthesized using sequential ovary-ovule culture on MS medium supplemented with casein hydrolysate. Morphological, molecular and cytogenetic analysis confirmed the true hybrid nature of the offspring. The F1 plants (2n = 18) were intermediate in morphology, highly pollen-sterile as well as self-sterile. A maximum of three bivalents per PMC was recorded, but 14 I + 2 II was the most common meiotic configuration. Normal pollen fertility and regular bivalent (18 II) formation was observed in the amphiploid sectors of the hybrid plants. The F1 hybrid harboured significantly lower aphid populations than the crop Brassica parent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01007.x
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  • 4
    Electronic Resource
    Electronic Resource
    Enarthrocarpus lyratus-based cytoplasmic male sterility and fertility restorer system in Brassica rapa (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Substituting the nuclear genome of Brassica rapa into the cytoplasmic background of Enarthrocarpus lyratus through backcross substitution helped in developing cytoplasmic male sterility (CMS). Alloplasmic male sterile plants had pale green leaves, small flowers with narrow petals and rudimentary anthers. Female fertility, low initially, improved considerably with advanced backcross generations. Male sterility expression was stable throughout the growing season. Except for EC 339014, all B. rapa accessions (38) evaluated were partial maintainers of the male sterility. Introgression of gene(s) for fertility restoration from the cytoplasm donor species was facilitated by homoeologous pairing between B. rapa and E. lyratus genomes, as was apparent from the very frequent occurrence of a trivalent in the monosomic addition plants (2n = 10 II + 1 I). Backcrossing of fertile monosomic addition plants with B. rapa led to the recovery of male fertile plants possessing the stable euploid chromosome number (2n = 20). These plants restored male fertility in crosses with different (lyr) CMS B. rapa genotypes, confirming the introgression of fertility restorer gene(s) from E. lyratus, the cytoplasm donor species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00836.x
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  • 5
    Electronic Resource
    Electronic Resource
    Tournefortii male sterility system in Brassica napus. Identification, expression and genetic characterization of male fertility restorers (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A germplasm collection of 152 diverse rapeseed accessions from Canada, China, France, India, Poland and South Korea was assayed for identifying new fertility restorers and sterility maintainers for a Tournefortii (tour) cytoplasmic male sterility (CMS) system in rape-seed. Only 16 (10.5%) genotypes showed complete fertility restoration following hybridization with tour CMS line NE 409A. Notable among these were GSL 8851, GSL 8953, Mokpo # 9, Mali, Buk-wuk-13, Kuju-27 and Mokpo # 84. As many as 78 (51.3%) genotypes were perfect maintainers of sterility, the remaining 58 (38.2%) genotypes were classified as partial maintainers. To study the inheritance of fertility restoration, 20 CMS (tour) rapeseed lines were crossed with the four best fertility restorers, namely GSL 8851, GSL 8953, Kuju-27 and Mokpo # 9, to obtain F2 and test cross populations. Segregation data indicated that fertility restoration for tour CMS was governed by two genes, of which, one is stronger than the other (χ212:3:1). Differences in gene interactions were also observed (χ29:3:4) which could be explained on the basis of influence of female parent genotypes/or modified expression of the restorer gene(s) in different genetic backgrounds. Tests of allelism indicated that the restorer genes present in the four restorers evaluated were allelic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00960.x
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  • 6
    Electronic Resource
    Electronic Resource
    Cytogenetic characterization of the 4BC region on the X chromosome of Drosophila melanogaster: Localization of the mei-9, norpA and omb genes (1986)
    Springer
    Chromosoma 93 (1986), S. 341-346 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Thirty genetic alterations, which involve the 4BC region of the Drosophila X chromosome, have been induced by ionizing radiation or by an endogenous mutator element. These mutations were recovered by screening for reversion of the dominant mutants Oce and Qd or for induction of the recessive mutants bi and rb. Among the 23 mutants generated by ionizing radiation, 20 have proven to be cytologically detectable chromosomal aberrations. Seven additional unique aberrations were generated in the Uc mutator strain. In total, 22 cytologically detectable deficiencies, 3 translocations, 1 inversion, 1 transposition, and 3 cytologically normal mutants have been recovered. Complementation analysis has permitted the cytogenetic localization of eight genes in the 4BC region. The mei-9 locus has been assigned to region 4B4-6, because this function is carried by Df (1)rb 41 but not by Df(1)bi D1. The norpA locus has been placed in the 4B6-C1 region based on its location between the distal breakpoints of Df(1)bi D2 and Df(1)rb 41. The genes lac, Qd, bi, and omb are localized to bands 4C5,6, rb to 4C6 and amb to 4C7,8. With one exception the complementation analysis has also permitted a determination of the linear sequence of these genes. This cytogenetic localization of these loci will facilitate the cloning and molecular analysis of genes controlling a key function in DNA repair and recombination (mei-9), and two fundamental neural functions (norpA and omb).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327593
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  • 7
    Electronic Resource
    Electronic Resource
    Male sterility in Indian mustard (Brassica juncea (L.) Coss.) — a biochemical characterization (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 515-519 
    Details
    ISSN: 1432-2242
    Keywords: Brassica juncea ; Male sterility ; Biochemical characterisation ; Microsporogenesis ; Peroxidase enzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Biochemical studies were conducted on some male sterile and their fertile F1 analogues in Indian mustard. The variation in pH activity during microsporogenesis was normal, except in MS-3. Male sterile anthers had deficient sugar metabolism. Cytochemical analysis of sporogenous and tapetal tissue suggested an effect of sterility elements on the anabolic and catabolic fate of DNA and protein during microsporogenesis. Leaves of male sterile lines had a higher chl a/b ratio. Leaf peroxidase activity was low and different isozymes appeared when separated by starch electrophoresis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264896
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  • 8
    Electronic Resource
    Electronic Resource
    Alternaria incidence in some alloplasmic lines of Indian mustard (Brassica juncea (L.) Coss.) (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 195-196 
    Details
    ISSN: 1432-2242
    Keywords: Indian mustard ; Brassica juncea ; Alternaria ; Alloplasm ; Nucleo-cytoplasmic interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytoplasmic substitution lines of Brassica juncea (L.) Coss were evaluated for their field resistance to Alternaria blight (Alternaria brassicae). The euplasmic B. juncea cv. ‘RLM 198’ had a mesothetic reaction while alloplasmic B. juncea lines with cytoplasms of B. campestris, B. chinensis, and B. japonica were highly susceptible. B. nigra cytoplasm did not have any effect on the disease reaction of the B. juncea genome. However, the alloplasmic lines with the cytoplasm of B. napus and B. carinata revealed a comparatively higher degree of resistance. The study underlined the utility of cytoplasmic manipulations in modifying the phenotypic expression of nuclear genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317032
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  • 9
    Electronic Resource
    Electronic Resource
    Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 116-122 
    Details
    ISSN: 1432-2242
    Keywords: Brassica juncea ; RAPD ; DNA polymorphism ; Genetic distance ; Heterosis breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RAPD assays were performed, using 34 arbitrary decamer oligonucleotide primers and six combinations of two primers, to detect inherent variations and genetic relationships among 12 Indian and 11 exotic B. juncea genotypes. Of 595 amplification products identified, 500 of them were polymorphic across all genotypes. A low level of genetic variability was detected among the Indian genotypes, while considerable polymorphism was present among the exotic ones. Based on the pair-wise comparisons of amplification products the genetic similarity was calculated using Jaccard's similarity coefficients and a dendrogram was constructed using an unweighted pair group method was arithmetical averages (UPGMA). On the basis of this analysis the genotypes were clustered into two groups, A and B. Group A comprised only exotic genotypes, whereas all the Indian genotypes and four of the exotic genotypes were clustered in group B. Almost similar genotypic rankings could also be established by computing as few as 200 amplification products. In general, a high per cent of heterosis was recorded in crosses involving Indian x exotic genotypes. On the other hand, when crosses were made amongst Indian or exotic genotypes, about 80% of them exhibited negative heterosis. Results from this study indicate that, despite the lack of direct correlation between the genetic distance and the degree of heterosis, genetic diversity forms a very useful guide not only for investigating the relationships among Brassica genotypes but also in the selection of parents for heterotic hybrid combinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222403
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  • 10
    Electronic Resource
    Electronic Resource
    Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of the male sterile line through somatic cell fusion (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 517-521 
    Details
    ISSN: 1432-2242
    Keywords: Ogu cytoplasmic male sterility ; Brassica juncea ; Chlorosis correction ; Protoplast fusion ; Mitochondrial recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Male sterility conferred by ogu cytoplasm of Raphanus sativus has been transferred to Brassica juncea cv ‘RLM 198’ from male-sterile B. napus through repeated backcrossing and selection. The male-sterile B. juncea is, however, highly chlorotic and late. It has low female (seed) fertility and small contorted pods. To rectify these defects, protoplasts of the male sterile were fused with normal ‘RLM 198’ (green, self fertile). Four dark green, completely male-sterile plants were obtained and identified as putative cybrids. All the plants were backcrossed three times with ‘RLM 198’. Mitochondrial and chloroplast DNA analysis of backcross progeny confirmed hybridity of the cytoplasm. The restriction pattern of the chloroplast DNA of progeny plants of three cybrids (Og 1, Og 2, Og 3) was similar to that of the green self-fertile ‘RLM 198’ and indicated that the correction of chlorosis resulted from chloroplast substitution. The chloroplast DNA of the lone progeny plant of the fourth cybrid (Og 10) could not be analyzed because the plant was stunted and had only a few leaves. When total cellular DNA was probed with mitochondrial probes coxI and atpA it was found that the cybrids had recombinant mitochondria. The chlorosis-corrected plants were early flowering and had vastly improved seed fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222982
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