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  • 1
    Electronic Resource
    Electronic Resource
    (−)-Maalian-5-ol, a new enantiomeric sesquiterpenoid from the liverwortPlagiochila ovalifolia (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1279-1280 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new enantiomeric sesquiterpene alcohol named (−)-maalian-5-ol was isolated from the liverwort, and the structure and absolute configuration was determined to be the stereostructureI by chemical and spectral evidence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01963955
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  • 2
    Electronic Resource
    Electronic Resource
    A new method for detection of anti-zona activity in human sera using latex agglutination reaction (1983)
    Springer
    Cellular and molecular life sciences 39 (1983), S. 926-928 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A slide latex test for detection of anti-zona pellucida activity in human sera was developed using a latex agglutination reaction. The latex reagent, made of polystyrene particles coated with solubilizing zona antigen(s), was found to give results comparable in sensitivity as well as specificity to those of the indirect immunofluorescence method as tested with anti-pig-zona antiserum and with human sera. Thus, the slide latex test was judged to be adequate for use instead of the indirect immunofluorescence technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01990442
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  • 3
    Electronic Resource
    Electronic Resource
    Cell migration within the embryonic limb primordium of Drosophila as revealed by a novel fluorescence method to visualize mRNA and protein (1997)
    Springer
    Development genes and evolution 207 (1997), S. 194-198 
    Details
    ISSN: 1432-041X
    Keywords: Key words Fluorescent probes ; In situ hybridization ; Distal-less ; Imaginal disc ; Confocal microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We report a new technique using fluorescent probes to detect a mRNA and a protein simultaneously in the Drosophila embryo. For in situ hybridization, 3-hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HNPP)/Fast Red TR was used as a fluorescent substrate for alkaline phosphatase. It was possible to compare protein and mRNA expression on a cell by cell basis with a laser scanning confocal microscope. We applied this technique to analyse the dynamics of Distal-less (Dll) enhancer activity in the thoracic limb primordium in the early Drosophila embryo. We stained embryos bearing the Dll early enhancer (Dll-304) fused to the Escherichia coli lacZ gene. LacZ mRNA was detectable in the ventral region of the limb primordium, and β-galactosidase protein in the dorsal region. In the middle, both mRNA and protein were detectable. These results suggest that the Dll enhancer is activated in the ventral region of the limb primordium and that Dll-positive cells migrate from a ventral position to a dorsal one within a single limb primordium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050107
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  • 4
    Electronic Resource
    Electronic Resource
    Hybridization of tRNAs of Drosophila melanogaster to the region of the 5S RNA genes of the polytene chromosomes (1981)
    Springer
    Chromosoma 82 (1981), S. 385-397 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have previously reported that four tRNAs of Drosophila melanogaster randomly labeled with iodine-125 hybridize in part to the 56EF region of polytene chromosomes where 5S RNA genes occur. In the presence of a 100-fold excess of unlabeled 5S RNA no hybridization of randomly labeled 125I-tRNAAsp 2γ occurred at 56EF although hybridization elsewhere was not affected. In addition, tRNAAsp 2γ labeled by introducing 125I-5-iodocytidylyl residues into the 3′-CCA end with tRNA nucleotidyl transferase did not hybridize to 56EF but did hybridize to its other sites. The hybridization of tRNALys 2, tRNAGly 3 and tRNAMet 3 at 56EF was not eliminated by a 25 to 100-fold excess of unlabeled 5S RNA. When these tRNAs were labeled at the -CCA terminus they hybridized to 56EF as well as to their other sites with the exception that terminally labeled tRNALys 2 no longer hybridized to 62A. The hybridization of the latter three species of tRNA to the region of the 5S genes, amongst other sites, is confirmed. The previously observed hybridization of tRNAAsp 2γ in this region appears to have been due to contamination of the tRNA sample with traces of material derived from 5S RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285764
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  • 5
    Electronic Resource
    Electronic Resource
    Chromosomal structure is altered by mutations that suppress or enhance position effect variegation (1990)
    Springer
    Chromosoma 99 (1990), S. 391-400 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We examined the genetic, morphological, and molecular effects of position effect variegation inDrosophila, and the effects of mutations that either suppress [Su(var)] or enhance [E(var)] this phenomenon. All eightSu(var) mutations examined strongly suppress the inactivation of variegating alleles of the genes white [In(l) w m4 ], brown [In (2R)bw VDe2 ] and Stubble [T(2;3)Sb V ]. TheE(var) mutation enhances variegation of these loci. The chromosomal region 3C-E (26 bands) which includes the white locus is usually packaged as heterochromatin in salivary glands of the variegating strainw m4 . Addition of any of theSu(var) mutations restores a more euchromatic morphology to this region. In situ hybridization to polytene chromosomes and DNA blot analyses of gene copy number demonstrate that the DNA of thew + gene is less accessible to its probe in the variegatingw m4 strain than it is in the wildtype or variegation-suppressed strains. Blot analysis of larval salivary gland DNA indicates that the white gene copy number does not vary among the strains. Hence, the differences in binding of thew + gene probe in the variegating and variegation-suppressed strains reflect differences in chromosomal packaging rather than alterations in gene number. The effects of variegation and theSu(var) mutations on chromatin structure were analyzed further by DNAse I digestion and DNA blot hybridization. In contrast to their dramatic effects on chromosomal morphology and gene expression, theSu(var) mutations had negligible effects on nuclease sensitivity of the white gene chromatin. We suggest that the changes in gene expression resulting from position effect variegation and the action of theSu(var) mutations involve alterations in chromosomal packaging.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01726690
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  • 6
    Electronic Resource
    Electronic Resource
    Electrophoretic studies of extracellular glucosyltransferases and fructosyltransferases from seventeen strains of Streptococcus mutans (1987)
    Springer
    Archives of microbiology 147 (1987), S. 207-212 
    Details
    ISSN: 1432-072X
    Keywords: Streptococcus mutans ; Glucosyltransferase ; Fructosyltransferase ; Electrophoresis ; Isoelectric focussing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Streptococcus mutans was classified by the electrophoretic properties of glucosyltransferases (GTases) and fructosyltransferases (FTases). The cells of serotypes a, d and g did not release extracellular FTases, although those from other serotypes did. The enzymes from cells of serotypes d and g synthesized a good deal of insoluble polysaccharide compared with other serotypes. The enzymes were applied to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and polyacrylamide gel-isoelectric focussing (PAG-IEF). Gels were stained for their activity and protein content. Enzymes belonging to the same serotype gave the same specific pattern on both gels. The seven serotypes could be classified into the following four groups: serotypes d and g, serotype a, serotypes c, e and f, and serotype b. The results agree well with some previous reports based on other methods. The molecular weights of three GTase bands were 156K, 146K and 135K, and of four kinds of FTase bands were 108K, 95K, 80K and 76K. The isoelectric points of main enzymes were 4.25, 4.60, 5.00, 5.55 and 5.70. Those of FTases were 4.25 and 4.60.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00463476
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  • 7
    Electronic Resource
    Electronic Resource
    Hybridization of tRNAs of Drosophila melanogaster to polytene chromosomes (1980)
    Springer
    Chromosoma 76 (1980), S. 65-84 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Highly purified tRNAs from Drosophila melanogaster were iodinated with 125I and hybridized to squashes of polytene chromosomes of Drosophila salivary glands followed by autoradiography to localize binding sites. Most tRNAs hybridize strongly to more than one site and weakly to one or more additional sites. The major sites for various tRNAs are the following: tRNA 2 Arg , 42A, 84F1,2; tRNA 2 Asp , 29DE; tRNA 3 Gly , 22BC, 35BC, 57BC; tRNA 2 Lys , 42A, 42E; tRNA 5 Lys , 84AB, 87B; tRNA 2 Met , 48B5–7, 72F1–2, 83F-84A; tRNA 3 Met , 46A1–2, 61D1–2, 70F1–2; tRNA 4 Ser , 12DE, 23E; tRNA 7 Ser , 12DE, 23E; tRNA 3a Val , 64D; tRNA 3b Val , 84D3–4, 92B1–9; tRNA 4 Val , 56D3–7, 70BC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292227
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  • 8
    Electronic Resource
    Electronic Resource
    Localization of tRNA genes of Drosophila melanogaster by in situ hybridization (1982)
    Springer
    Chromosoma 86 (1982), S. 279-292 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Six purified tRNAs labeled with 125I by chemical or enzymatic methods were hybridized to polytene chromosomes of Drosophila melanogaster. The main chromosomal regions of hybridization were: tRNA GGA Gly , 58A, 84C, and 90E; tRNA 2 Leu , 44E, 66B5-8, and 79F; tRNA 2b Ser , 86A, 88A9-12, and 94A6-8; tRNA 3 Thr , 47F and 87B; tRNA 4 Thr , 93A1-2; and tRNA 1γ Tyr , 19F, 22F-23A, 41, 50C1-4 and 85A. At 50C the hybridization of tRNA 1γ Tyr was polymorphic in the giant strains. When the hybridization of three valine isoacceptors studied previously was re-investigated, it was found that only one hybridization site, 90BC, was shared between tRNA 3b Val and tRNA 4 Val . tRNA 3a Val did not have any sites in common with the other two.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288682
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  • 9
    Electronic Resource
    Electronic Resource
    Cytofluorometric nuclear DNA determinations on the atrioventricular nodal cells in human hearts (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 111-115 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the human heart, it is well known that the polyploidization of working heart-muscle cells increases in proportion to increases in heart weight, but there has been no investigation of the process of polyploidization in the specialized heart-muscle cells of the cardiac conduction system which have a nerve-like function. In order to investigate the process of polyploidization in these cells, the nuclear DNA content of atrioventricular nodal cells was measured using cytofluorometry. Tissue samples taken from autopsied hearts without arrhythmias were embedded in paraffin blocks after Carnoy fixation. Blocks containing the atrioventricular conduction system were cut according to the serial sectioning method of Lev et al. The compact atrioventricular nodes were removed from thick paraffin sections (150 μm) under a stereomicroscope. The cells were then isolated by enzyme digestion and ultrasonic treatment. Smears of the isolated cells were double stained with azocarmin-G and acriflavine-Feulgen. Cytofluorometric DNA determinations of the DNA content of atrioventricular nodal cells were performed. Atrioventricular nodes were found to be composed of a large number of diploid cells and a small number of tetraploid cells. No octaploid cells were found. These findings reveal that the process of polyploidization in atrioventricular nodal cells is different from that found in working heart-muscle cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491756
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  • 10
    Electronic Resource
    Electronic Resource
    Phthalate esters ofOenanthe stolonifera DC. (Umbelliferae) (1969)
    Springer
    Cellular and molecular life sciences 25 (1969), S. 907-908 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Aus dem Extrakt vonOenanthe stolonifera DC. (Umbelliferae) wurden n-Butyl-2-äthylbutyl-phthalat, di-2-äthylbutyl-phthalat und di-äthyl-phthalat isoliert und deren Struktur durch chemischen Abbau und mit Hilfe physikalischer Methoden aufgeklärt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01898054
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