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A new method for detection of anti-zona activity in human sera using latex agglutination reaction

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Summary

A slide latex test for detection of anti-zona pellucida activity in human sera was developed using a latex agglutination reaction. The latex reagent, made of polystyrene particles coated with solubilizing zona antigen(s), was found to give results comparable in sensitivity as well as specificity to those of the indirect immunofluorescence method as tested with anti-pig-zona antiserum and with human sera. Thus, the slide latex test was judged to be adequate for use instead of the indirect immunofluorescence technique.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, School of Medicine, Kyoto University, 54 Shyogoin Kawahara-cho, Sakyo-ku, 606, Kyoto, Japan

    Y. Noda, I. Yamada, S. Hayashi, I. Takai & E. Watanabe

  2. Daigo-Watanabe Womens Hospital, Takahata-cho, Daigo, 601-13, Kyoto, Japan

    Y. Noda, I. Yamada, S. Hayashi, I. Takai & E. Watanabe

Authors
  1. Y. Noda
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  2. I. Yamada
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  3. S. Hayashi
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  4. I. Takai
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  5. E. Watanabe
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Noda, Y., Yamada, I., Hayashi, S. et al. A new method for detection of anti-zona activity in human sera using latex agglutination reaction. Experientia 39, 926–928 (1983). https://doi.org/10.1007/BF01990442

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  • DOI: https://doi.org/10.1007/BF01990442

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