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  • Articles  (3)
  • Articles: DFG German National Licenses  (3)
  • Springer  (3)
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  • International Union of Crystallography (IUCr)
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  • Process Engineering, Biotechnology, Nutrition Technology  (3)
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  • Articles  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Biotransformation of linoleic acid with the Candida tropicalis M25 mutant (1997)
    Springer
    Applied microbiology and biotechnology 48 (1997), S. 83-87 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Linoleic acid was transformed by mutant Candida tropicalis M25 and transformations were studied in batch and fed-batch cultures. Cofermentations with palmitic acid as inducer of the fatty acid degradation pathway were performed. Besides the (Z),(Z)-octadeca-6,9-dienedioic acid, (Z),(Z)-3-hydroxyoctadeca-9,12-dienedioic acid and (Z),(Z)-3-hydroxytetradeca-5,8-dienedioic acid were obtained as the main fermentation products. The maximum concentrations of (Z),(Z)-octadeca-6,9-dienedioic acid and (Z),(Z)-3-hydroxyoctadeca-9,12-dienedioic acid reached values of 6.4 g/l and 6.9 g/l respectively. The structures of the products were characterized by chemical and spectroscopic methods. The configuration of the double bonds was not changed during bioconversion. As only one regioisomer of the hydroxylated fatty acid was detected, the hydroxylation is site-specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051019
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  • 2
    Electronic Resource
    Electronic Resource
    Identification and production of 3-hydroxy-Δ9-cis-1,18-octadecenedioic acid by mutants of Candida tropicalis (1996)
    Springer
    Applied microbiology and biotechnology 45 (1996), S. 342-348 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The transformation of oleic acid by mutants of Candida tropicalis was studied in fed-batch cultures. Besides Δ9-cis–1,18-octadecenedioic acid, 3-hydroxy-Δ9-cis–1,18-octadecenedioic acid was detected as the main fermentation product. Here we describe the production, isolation and the complete chemical characterization of the purified 3-hydroxy-Δ9-cis–1,18-octadecenedioic acid. The geometric configuration of the double bond was not changed during bioconversion. The enantiomeric excess of the compound was 76%. Mutagenesis of C. tropicalis DSM 3152 with N-methyl-N-nitro-N′-nitrosoguanidine and selection with oleic acid as the sole carbon source led to mutant M 25, which produced the 3-hydroxy-Δ9-cis–1,18-octadecenedioic acid at a 1.8-fold higher concentration in the medium as compared to the parent strain. The maximum concentration of the hydroxy dioic acid was 19.4 g/l after 223 h fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050694
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  • 3
    Electronic Resource
    Electronic Resource
    Bioconversion of sunflower oil, rapeseed oil and ricinoleic acid by Candida tropicalis M25 (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 573-578 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Bioconversions of sunflower oil and rapeseed oil in fed-batch cultures fermented with Candida tropicalis M25 were studied. Cofermentations with palmitic acid resulted in successful transformations to different 3-hydroxydioic acids. The absolute configuration of the major fermentation product, R-(Z)-3-hydroxy-9-octadecenedioic acid, was determined by comparison of the 1H NMR data with those of an authentic, optically pure compound. Using ricinoleic acid as the sole carbon source, the optically pure R-(Z)-7-hydroxy-9-octadecenedioic acid was obtained at a maximum concentration of 4.2 g/l. In addition to the hydroxydioic acid, (Z)-3,12-dihydroxy-9-octadecenedioic acid was also produced following fermentation with the mutant M25.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051337
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