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  • Articles  (15)
  • Articles: DFG German National Licenses  (15)
  • Springer  (15)
  • 1990-1994  (15)
  • 1
    Electronic Resource
    Electronic Resource
    GaAs multiple quantum well microresonator modulators grown on silicon substrates (1992)
    Springer
    Optical and quantum electronics 24 (1992), S. S177 
    Details
    ISSN: 1572-817X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract We report new results on the modulation characteristics of GaAs/AlGaAs asymmetric Fabry-Perot modulators grown on silicon substrates. We discuss factors affecting device performance and evaluate these by growing p-i-n quantum well diodes, and multilayer reflector stacks on silicon. Using data from these test structures we have designed an asymmetric microresonator modulator and achieve, experimentally, a 40% reflection change with only 5 V and a contrast ratio of 7.4 dB, also with 5 V.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00625823
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  • 2
    Electronic Resource
    Electronic Resource
    A genetic analysis of glucoamylase activity in the diastatic yeast Saccharomyces cerevisiae NCYC 625 (1990)
    Springer
    Current genetics 17 (1990), S. 281-288 
    Details
    ISSN: 1432-0983
    Keywords: Glucoamylase ; Gene-mapping ; STA2 ; Mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear suppressor genes (SGL) found in some S. cerevisiae tester strains. EMS-induced glucoamylase-negative mutants often contain STA2-suppressor mutations. Depending on the allelic status of GEP1, a nuclear gene which also appears able to antagonise SGL-mediated suppression, STA2 expression can be blocked in petite mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314873
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning of theHIS3 gene ofYarrowia lipolytica (1991)
    Springer
    Antonie van Leeuwenhoek 60 (1991), S. 95-99 
    Details
    ISSN: 1572-9699
    Keywords: HIS3 ; Yarrowia lipolytica ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheHIS3 gene of the yeastYarrowia lipolytica has been cloned from a genomic library by complementation of thehis3 mutation ofSaccharomyces cerevisiae. The gene was subsequently subcloned inEscherichia coli and characterized by restriction enzyme mapping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572698
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  • 4
    Electronic Resource
    Electronic Resource
    Antiresorptive drugs and trabecular bone turnover: Validation and testing of a computer model (1994)
    Springer
    Calcified tissue international 54 (1994), S. 179-185 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Drugs ; Trabecular ; Turnover ; Computer ; Model ; Sensitivity ; Activation frequency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract A computer model of trabecular bone turnover has been developed, based on concepts of Jonathan Reeve [1]. This model predicts changes in bone volume by summing bone resorption and formation over a large number of remodeling sites. Clinical data [histomorphometry and bone mineral content (BMC)] from two clinical studies using an antiresorptive drug (etidronate disodium, EHDP) in post-menopausal osteoporosis were used to test the model. The results for BMC obtained from the EHDP and placebo groups in each study at 60 and 120 weeks were correctly predicted by the model from the histomorphometric data obtained from baseline and week 60 biopsies. The parameter in this model having the greatest influence on predicted changes in bone volume was found by sensitivity analysis to be activation frequency. These results suggest that the contribution of bone turnover to BMC can be predicted solely by considering the cell kinetics of the basic multicellular unit (BMU), and that, in the case of antiresorptive drugs, maximal effects on bone volume may be achieved by pharmacological reduction of activation frequency. The results also suggest that the present model may be useful in predicting in clinical studies the effects of EHDP and similar drugs on bone turnover.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301675
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  • 5
    Electronic Resource
    Electronic Resource
    DNA-binding properties of cloned TATA-binding protein from potato tubers (1992)
    Springer
    Plant molecular biology 19 (1992), S. 455-464 
    Details
    ISSN: 1573-5028
    Keywords: transcription ; cDNA cloning ; TATA-binding protein ; TFIID ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA clone encoding the TATA-binding protein (TBP), the DNA-binding component of the general transcription factor TFIID was cloned from potato tubers. The DNA sequence of this cDNA indicated that the predicted potato protein was very similar to cloned TBP from other species. Genomic southern analysis showed that TBP is encoded in the potato genome as a low-copy-number sequence. The potato TBP cDNA clone was shown to encode a functional protein that interacts in a sequence-specific way with the promoter region of a class-1 potato patatin gene. Functional analysis of carboxyterminal truncated derivatives of potato TBP showed that important components of DNA binding were located within the carboxy-terminal 54 amino acids. Kinetic and thermodynamic properties of in vitro synthesised potato TBP were investigated, and demonstrated strict salt and temperature preferences for maximum DNA binding activity. In addition on and off-rate measurements showed that both association and dissociation of TBP from DNA is slow. The specific and the non-specific equilibrium constants K s and K n were calculated as 5×109 M-1 and 3.65×104 M-1 respectively. These results indicate that the interaction of potato TBP with the patatin promoter is highly specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023393
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  • 6
    Electronic Resource
    Electronic Resource
    Transcriptional regulation of a patatin-1 gene in potato (1990)
    Springer
    Plant molecular biology 14 (1990), S. 995-1006 
    Details
    ISSN: 1573-5028
    Keywords: patatin ; β-glucuronidase ; tuber formation ; sucrose ; transgenic potatoes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Patatin is an abundant glycoprotein in the tubers of potato plants that has a lipid acyl hydrolase activity. Fusions of the promoter of patatin genes that are highly expressed in tubers with the reporter gene encoding β-glucuronidase (GUS) have shown that patatin transcription has a high degree of tuber specificity. Patatin transcription was also inducible in other organs of transgenic potato by growth on high concentrations of sucrose. Experiments were conducted to define regions of the patatin promoter that confered tuber specific expression and sucrose inducibility. Sequences between -40 and -400 bp and between -400 and -957 bp of the transcriptional start site were able to confer tuber-specific expression on a heterologous truncated promoter. The cell specificity of GUS transcription in the transformants indicated that organ specificity was possibly determined by source-sink relationships of sucrose, or a metabolite of sucrose, in the whole plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019396
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  • 7
    Electronic Resource
    Electronic Resource
    New patterns of gene activity in plants detected using an Agrobacterium vector (1991)
    Springer
    Plant molecular biology 16 (1991), S. 263-269 
    Details
    ISSN: 1573-5028
    Keywords: transformation ; enhancer trap ; β-glucuronidase ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding β-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were detected at high frequency using in situ assays for GUS activity. Previous studies in Drosophila using analogous vectors have shown that the new patterns of transcription in many cases reflect the patterns of expression of genes adjacent to the site of vector insertion. If this is also the case in plants, the vector described here will be useful in identifying the activity of genes in different cell types and will assist in determining their function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020557
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  • 8
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of a β-d-galactoside-binding lectin at the human maternofetal interface (1994)
    Springer
    Journal of molecular histology 26 (1994), S. 582-586 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The 14 kD S-type lectin from human placenta may have a role in regulating the maternal immune response to fetal antigens. In this study, an immunoperoxidase technique was used to determine the distribution of the lectin at the human maternofetal interface. Tissue obtained during the first trimester of pregnancy and at term was used. The lectin was not detectable in either the villous syncytiotrophoblast or the underlying cytotrophoblast in first-trimester tissue, although some cells of the cytotrophoblast columns were reactive. It was also not detectable in villous or extravillous trophoblast populations at term. In contrast, strong reactivity was found in decidual stromal cells throughout gestation, and endometrial stromal cells were also positive. The lectin is, therefore, not a component of the immunosuppressive factors associated with syncytiotrophoblast membranes, but may have a role in either the decidual control of trophoblast migration or some functions unrelated to pregnancy, or both.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00158592
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  • 9
    Electronic Resource
    Electronic Resource
    Partial characterization of the Nicotiana tabacum actin gene family: Evidence for pollen-specific expression of one of the gene family members (1993)
    Springer
    Molecular genetics and genomics 240 (1993), S. 290-295 
    Details
    ISSN: 1617-4623
    Keywords: Tobacco ; Nicotiana tabacum ; Actin ; Pollen ; Gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The actin gene family of Nicotiana tabacum has been partially characterised by Southern hybridisation and by isolating λEMBL4 recombinants from a genomic library having homology to the soybean actin gene, Sac3. The number of actin genes with homology to Sac3 is estimated at between 20 to 30, based on Southern hybridisation and library screening, though the total gene family may be larger. Twenty-four recombinant λ clones were isolated, 18 had unique restriction profiles and from these, 2 clones, Tac9 and Tac25, were selected for further study. The region of Tac25 hybridizing to Sac3 was sequenced and shown to contain an open reading frame (ORF) with homology to actin. Partial sequencing of Tac9 revealed a sequence with homology to the third exon of Tac25 and Sac3. The two tobacco actin sequences were compared to other reported actin gene sequences; Tac25 was closely related to the allelic potato actins, Pac58 and Pac85, while Tac9 was more related to Pac79 than to other plant actins. Northern hybridisation analysis showed that while Tac9 detected actin transcripts in RNA from root, leaf, stigma and pollen, Tac25 transcripts were only detected in pollen RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277069
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  • 10
    Electronic Resource
    Electronic Resource
    Mechanisms and micromechanics of fatigue crack propagation in glassy thermoplastics (1990)
    Springer
    Colloid & polymer science 268 (1990), S. 814-822 
    Details
    ISSN: 1435-1536
    Keywords: Fatigue ; crackpropagation ; thermoplastics ; crazestress ; crazeextensionratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An iterative approach is used to estimate, from interference optics measurements, the variation of refractive index and, hence, extension ratio along the length of a craze at the tip of a fatigue crack. The finite element method is used to compute craze surface stress distributions which are found to be similar to those obtained for static loading. High extension ratios, in the range 6 to 8 for retarded fatigue crack growth in poly(vinylchloride), are attained in the craze fibrils at the crack tip before crack jump occurs. The craze thickens primarily by surface drawing during the early stages of its growth but in the later stages the fibril creep mechanism predominates. The critical fibril extension ratio is not reached in a single cycle, as in normal fatigue crack propagation, and crack jump does not occur until, typically, after several hundreds of cycles during which the fibrils accumulate considerable damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01410959
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