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  • Articles  (1,536)
  • Articles: DFG German National Licenses  (1,536)
  • 2000-2004  (619)
  • 1950-1954  (380)
  • 1930-1934  (285)
  • 1925-1929  (252)
  • Process Engineering, Biotechnology, Nutrition Technology  (1,218)
  • Geography  (318)
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  • Articles  (1,536)
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  • 1
    Electronic Resource
    Electronic Resource
    Spatial and temporal variability in forest–atmosphere CO2 exchange (2004)
    Oxford, UK : Blackwell Science Ltd
    Global change biology 10 (2004), S. 0 
    Details
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Seven years of carbon dioxide flux measurements indicate that a ∼90-year-old spruce dominated forest in Maine, USA, has been sequestering 174±46 g C m−2 yr−1 (mean±1 standard deviation, nocturnal friction velocity (u*) threshold 〉0.25 m s−1). An analysis of monthly flux anomalies showed that above-average spring and fall temperatures were significantly correlated with greater monthly C uptake while above-average summer temperatures were correlated with decreased net C uptake. Summer months with significantly drier or wetter soils than normal were also characterized by lower rates of C uptake. Years with above-average C storage were thus typically characterized by warmer than average spring and fall temperatures and adequate summer soil moisture.Environmental and forest–atmosphere flux data recorded from a second tower surrounded by similar forest, but sufficiently distant that flux source regions (‘footprints’), did not overlap significantly showed almost identical temperature and solar radiation conditions, but some differences in energy partitioning could be seen. Half-hourly as well as integrated (annual) C exchange values recorded at the separate towers were very similar, with average annual net C uptake differing between the two towers by 〈6%. Interannual variability in net C exchange was found to be much greater than between tower variability. Simultaneous measurements from two towers were used to estimate flux data uncertainty from a single tower. Carbon-flux model parameters derived independently from each flux tower data set were not significantly different, demonstrating that flux towers can provide a robust method for establishing C exchange model parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2486.2004.00847.x
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  • 2
    Electronic Resource
    Electronic Resource
    Response of tundra ecosystem in southwestern Alaska to Younger-Dryas climatic oscillation (2002)
    Oxford, UK : Blackwell Science Ltd
    Global change biology 8 (2002), S. 0 
    Details
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Climatic warming during the last glacial–interglacial transition (LGIT) was punctuated by reversals to glacial-like conditions. Palaeorecords of ecosystem change can help document the geographical extent of these events and improve our understanding of biotic sensitivity to climatic forcing. To reconstruct ecosystem and climatic variations during the LGIT, we analyzed lake sediments from southwestern Alaska for fossil pollen assemblages, biogenic-silica content (BSiO2%), and organic-carbon content (OC%). Betula shrub tundra replaced herb tundra as the dominant vegetation of the region around 13 600 cal BP (cal BP: 14C calibrated calendar years before present), as inferred from an increase of Betula pollen percentages from 〈〈 5% to 〉〉 20% with associated decreases in Cyperaceae, Poaceae, and Artemisia. At c. 13 000 cal BP, a decrease of Betula pollen from 28 to 〈〈 5% suggests that shrub tundra reverted to herb tundra. Shrub tundra replaced herb tundra to resume as the dominant vegetation at 11 600 cal BP. Higher OC% and BSiO2% values suggest more stable soils and higher aquatic productivity during shrub-tundra periods than during herb-tundra periods, although pollen changes lagged behind changes in the biogeochemical indicators before c. 13 000 cal BP. Comparison of our palaeoecological data with the ice-core dδ18O record from Greenland reveals strikingly similar patterns from the onset through the termination of the Younger Dryas (YD). This similarity supports the hypothesis that, as in the North Atlantic region, pronounced YD climatic oscillations occurred in the North Pacific region. The rapidity and magnitude of ecological changes at the termination of the YD are consistent with greenhouse experiments and historic photographs demonstrating tundra sensitivity to climatic forcing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2486.2002.00550.x
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  • 3
    Electronic Resource
    Electronic Resource
    Water use of two Mojave Desert shrubs under elevated CO2 (2000)
    Oxford, UK : Blackwell Science Ltd
    Global change biology 6 (2000), S. 0 
    Details
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Plant responses to elevated atmospheric CO2 have been characterized generally by stomatal closure and enhanced growth rates. These responses are being increasingly incorporated into global climate models that quantify interactions between the biosphere and atmosphere, altering climate predictions from simpler physically based models. However, current information on CO2 responses has been gathered primarily from studies of crop and temperate forest species. In order to apply responses of vegetation to global predictions, CO2 responses in other commonly occurring biomes must be studied. A Free Air CO2 Enrichment (FACE) study is currently underway to examine plant responses to high CO2 in a natural, undisturbed Mojave Desert ecosystem in Nevada, USA. Here we present findings from this study, and its companion glasshouse experiment, demonstrating that field-grown Ephedra nevadensis and glasshouse-grown Larrea tridentata responded to high CO2 with reductions in the ratio of transpirational surface area to sapwood area (LSR) of 33% and 60%, respectively. Thus, leaf-specific hydraulic conductivity increased and stomatal conductance remained constant or was increased under elevated CO2. Field-grown Larrea did not show a reduced LSR under high CO2, and stomatal conductance was reduced in the high CO2 treatment, although the effect was apparent only under conditions of unusually high soil moisture. Both findings suggest that the common paradigm of 20–50% reductions in stomatal conductance under high CO2 may not be applicable to arid ecosystems under most conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2486.2000.00360.x
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  • 4
    Electronic Resource
    Electronic Resource
    AN ORGANIZED SIGNAL IN SNOWMELT RUNOFF OVER THE WESTERN UNITED STATES (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 36 (2000), S. 0 
    Details
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : Daily-to-weekly discharge during the snowmelt season is highly correlated among river basins in the upper elevations of the central and southern Sierra Nevada (Carson, Walker, Tuolumne, Merced, San Joaquin, Kings, and Kern Rivers). In many cases, the upper Sierra Nevada watershed operates in a single mode (with varying catchment amplitudes). In some years, with appropriate lags, this mode extends to distant mountains. A reason for this coherence is the broad scale nature of synoptic features in atmospheric circulation, which provide anomalous insolation and temperature forcing that span a large region, sometimes the entire western U.S. These correlations may fall off dramatically, however, in dry years when the snowpack is spatially patchy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1752-1688.2000.tb04278.x
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  • 5
    Electronic Resource
    Electronic Resource
    L-moments and C-moments (2000)
    Springer
    Stochastic environmental research and risk assessment 14 (2000), S. 50-68 
    Details
    ISSN: 1436-3259
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract  It is well known that the computation of higher order statistics, like skewness and kurtosis, (which we call C-moments) is very dependent on sample size and is highly susceptible to the presence of outliers. To obviate these difficulties, Hosking (1990) has introduced related statistics called L-moments. We have investigated the relationship of these two measures in a number of different ways. Firstly, we show that probability density functions (pdf ) that are estimated from L-moments are superior estimates to those obtained using C-moments and the principle of maximum entropy. C-moments computed from these pdf's are not however, contrary to what one may have expected, better estimates than those estimated from sample statistics. L-moment derived distributions for field data examples appear to be more consistent sample to sample than pdf 's determined by conventional means. Our observations and conclusions have a significant impact on the use of the conventional maximum entropy procedure which typically uses C-moments from actual data sets to infer probabilities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004770050004
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  • 6
    Electronic Resource
    Electronic Resource
    Field tests on managing resistance to Bt -engineered plants (2000)
    [s.l.] : Nature America Inc.
    Nature biotechnology 18 (2000), S. 339-342 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Several important crops have been engineered to express toxins of Bacillus thuringiensis (Bt) for insect control. In 1999, US farmers planted nearly 8 million hectares (nearly 20 million acres) of transgenic Bt crops approved by the EPA. Bt-transgenic plants can greatly reduce the use of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/73804
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  • 7
    Electronic Resource
    Electronic Resource
    Sequence of PHA synthase gene from two strains of Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across two heterologous expression systems (2000)
    Springer
    Applied microbiology and biotechnology 53 (2000), S. 420-429 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A 3.0-kb genomic fragment has been isolated from Rhodospirillum rubrum (ATCC 25903) that contains an open reading frame (ORF) with strong homology to other known polyhydroxyalkanoate (PHA) synthase genes. This ORF has lower homology to the R. rubrum strain Ha PHA synthase than would be expected within the same species. We have conducted a series of heterologous expression studies evaluating the in vivo substrate specificity of PHA synthase genes from Rhodobacter sphaeroides, Ralstonia eutropha (formerly Alcaligenes eutrophus), Thiocystis violacea, and Nocardia corrallina, within the PHA-synthase-negative hosts, Ralstonia eutropha DSM541 and Pseudomonas putida GpP104. The N. corrallina PHA synthase incorporated the highest percentage of C5 monomers in the polymer when fermented in medium supplemented with 0.1% heptanoate as the sole carbon source. When the T. violacea and R. sphaeroides were expressed in the PHA-negative host DSM541, a greater percentage of C5 monomer was observed in the polymer as compared to the expression of the PHA synthase of R. eutropha, when the transconjugants were fermented in medium supplemented with 0.4% propionate. Evaluation for preference of medium-chain-length monomers demonstrated the flexibility of the N. corrallina, T. violacea, and R. eutropha synthase genes to polymerize a copolyester composed of short- and medium-chain-length monomers when the respective transconjugants were fermented in medium supplemented with 0.5% octanoate. These studies demonstrate that the PHA synthase from N. corrallina, T. violacea, and R. eutropha are able to polymerize a copolyester composed of short- and medium-chain-length monomers, while the PHA synthase from R. sphaeroides lacks this ability and only produces a short-chain-length polymer. These observations suggest that the composition of the PHA from the PHA-producing organisms does not necessarily reflect the inherent specificity of the PHA synthase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051636
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  • 8
    Electronic Resource
    Electronic Resource
    Purification, characterization, and primary structure of a chitinase from Pseudomonas sp. YHS-A2 (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 397-405 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A chitinase gene (chiA) from Pseudomonas sp. YHS-A2 was cloned into Escherichia coli using pUC19. The nucleotide sequence determination revealed a single open reading frame of chiA comprised of 1902 nucleotide base pairs and 633 deduced amino acids with a molecular weight of 67,452 Da. Amino acid sequence alignment showed that ChiA contains two putative chitin-binding domains and a single catalytic domain. Two proline-threonine repeat regions, which are linkers between catalytic and substrate-binding domains in some cellulases and xylanases, were also found. From E. coli, ChiA was purified 12.8-fold relative to the periplasmic fraction. The Michaelis constant and maximum initial velocity for p-nitrophenyl-N,N′-diacetylchitobiose were 1.06 mM and 44.4 μmol/h per mg protein, respectively. The purified ChiA binds not only to colloidal chitin but also to other substrates (avicel, chitosan, and xylan), but the binding affinity of avicel, chitosan, and xylan is around 10 times lower than that of colloidal chitin. The reaction of ChiA with colloidal chitin and chitooligosaccharides (trimer-hexamer) produced an end product of N,N′-diacetylchitobiose, indicating that ChiA is a chitobiosidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000408
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  • 9
    Electronic Resource
    Electronic Resource
    Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 659-664 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM), was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS•) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS• was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS• in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000436
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  • 10
    Electronic Resource
    Electronic Resource
    A new physical test for vulcanized rubber (1929)
    s.l. : American Chemical Society
    Industrial and engineering chemistry 1 (1929), S. 17-20 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac50065a007
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