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  • Articles  (51)
  • Latest Papers from Table of Contents or Articles in Press  (40)
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  • Articles  (51)
Source
  • Latest Papers from Table of Contents or Articles in Press  (40)
  • Articles: DFG German National Licenses  (11)
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  • 1990-1994  (24)
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  • 1
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 37 (1991), S. 1789-1800 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The finite element method was used to model microwave thawing of pure-water and 0.1-M NaCl cylinders. The electromagnetic field was described by Maxwell's equations with temperature-dependent dielectric properties, while the heat equation, coupled with the Stefan and Robin conditions, was used to describe the thawing process. An additional equation for the frozen volume fraction was used, when necessary, to account for the presence of a mushy region. Two microwave frequencies, 915 MHz and 2,450 MHz, were examined and the microwave radiation was assumed to be radially isotropic and normal to the surface of the cylinder. Results show that a two-phase mushy region may exist, and an additional thawing front may appear at the center of the cylinder. Salt cylinders have a higher dielectric loss than pure-water cylinders and therefore thaw more quickly. Internal resonance occurs when the wavelength of the radiation is a harmonic of the cylinder radius. Resonance increases power deposition and expedites the thawing process. The onset of resonance alters thawing times and complicates the development of heuristic rules for microwave thawing.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 37 (1991), S. 313-322 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Transient temperature profiles in multilayer slabs are predicted, by simultaneously solving Maxwell's equations with the heat conduction equation, using Galerkin finite elements. It is assumed that the medium is homogeneous and has temperature-dependent dielectric and thermal properties. The method is illustrated with applications involving the heating of food and polymers with microwaves. The temperature dependence of dielectric properties affects the heating appreciably, as is shown by comparison with a constant property model.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 365-372 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The approximate equations of Hassler and Brunner and of van Domselaar are often used to deduce the capillary pressure curve of a porous medium from centrifuge data. The use of these equations restricts the centrifuge method to short samples. Also, these equations require differentiation of data. We report here methods to determine the capillary pressure curve by the midpoint and least-squares solution of the fundamental equation, relating the average saturation S1 of liquid in the porous medium to the capillary pressure Pc1 at the end of the sample nearest the axis of rotation. The methods do not require differentiation of data and are not restricted to short samples. We introduce and evaluate an approximation based on an exact result derived by Rajan. This new approximation requires the same inputs as do the Hassler-Brunner and the van Domselaar approximations, but it is accurate over a wider range of sample sizes. In addition, an approximate solution which can be used to estimate capillary pressure curves for long cores is developed.
    Additional Material: 6 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 37 (1991), S. 1905-1905 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 749-760 
    ISSN: 0006-3592
    Keywords: metabolic modeling ; bioreactor optimization ; recombinant protein synthesis ; induction dynamics ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The dynamics of chemically induced chloramphenicolaceyl-transferase (CAT) expression are determined in batch cultures of Escherichia coli DH5αF'IQ [pKK262-1]. This article is directed towards understanding the coupling of induced cloned-protein synthesis and reduced cell growth which are balanced in the optimal system. Experimental results indicate that the best inducer (IPTG) concentration is near 1.0 mM when added during midexponential growth. Lower concentrations cause only weak induction, whereas higher concentrations cause sufficiently strong induction that cell growth is suppressed. Induction at the onset of the stationary phase results in high expression but is accompanied by stimulated protease activity. Also, cell mass yield is adversely affected by enhanced protein synthesis. A structured metabolic model is shown to predict the responses of instantaneous growth rate and productivity which result from protein overexpression. This model can be employed to predict alternative reactor strategies and operating conditions necessary for the design of efficient bioprocess.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 1190-1202 
    ISSN: 0006-3592
    Keywords: continuous centrifugal bioreactor ; ethanol fermentation ; mammalian cell hybridoma ; monoclonal antibody ; fluidized bed ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A continuous centrifugal bioreactor (CCBR), developed to study the growth and productivity of dense suspensions cultures, has been applied to both fermentation and mammalian cell cultivation processes. With this approach, high-density nonflocculent cultures are maintained in a tapered fluidized bed by balancing the drag forces on the cells due to following substrate with the centrifugal forces. The Sysyem was first used to produce ethanol by fermentation with Saccharomyces cerevisiae; then with H21A1 mouse hybridoma cells secreting monoclonal antibody (MoAb), lgM. Results of this research show the feasibility of using the CCBR for both production of secreted products and as a research tool for studying cell metabolism and production kinetics. Media recycle may be used to modify the behavior of the system form a plug flow apparatus to a continuous stirred reactor (CSTR).
    Additional Material: 8 Ill.
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  • 7
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 767-776 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous fermentations were carried out involving competition between two strains of Saccharomyces cerevisiae. One of the strains has a lower specific growth rate and is very flocculent, whereas the fastergrowing strain is nonflocculent. The product stream from the chemostat was fed into an inclined settler where the flocculent strain was partially separated from the nonflocculent strain as a result of the higher sedimentation rate of the flocculent cells. The underflow from the inclined settler, which was concentrated and enriched with flocculent cells, was recycled to the chemostat. When no recycle was used, the fastergrowing, nonflocculent yeast rapidly overtook the culture. With selective recycle, however, the experiments demonstrated that the slower-growing flocculent yeast could be maintained as the dominant species. A theoretical development is also presented in order to describe the competition between two strains in the bioreactor-settler system. The concept of selective recycle via selective flocculation and sedimentation offers a possible means of maintaining unstable recombinant microorganisms in continuous fermentations.
    Additional Material: 9 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 37 (1991), S. 569-580 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Deep knowledge about process behaviors plays an important role in the diagnosis of chemical processes. Cause-and-effect reasoning using deep knowledge is useful especially for interacting malfunctions. This work explores the integration of deep knowledge into task-specific, knowledge-based architectures for resolving interacting multiple malfunctions and presents a novel methodology called diagnostically focused simulation (DFS). Invoked in an auxiliary manner, DFS uses deep knowledge and performs qualitative simulation in a highly constrained manner. The close integration with other problem solvers is an evolutionary approach to using qualitative simulation in diagnosis and manages a normally computationally-explosive procedure. Diagnostic results from the compiled problem solver provide a situation-specific assessment of the chemical process, identify possible malfunction scenarios, and focus on appropriate levels of process detail. DFS effectively demonstrates a balance between run-time simulation and compiled problem solving in diagnosis.
    Additional Material: 14 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 325-333 
    ISSN: 0006-3592
    Keywords: segregation ; flocculation ; induction ; fermentations, continuous ; protein overproduction ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new plasmid construct has been used in conjunction with selective recycle to successfully maintain otherwise unstable plasmid-bearing E. coli cells in a continuous bioreactor and to produce significant amounts of the plasmid-encoded protein β-lactamase. The plasmid is constructed so that pilin expression, which leads to bacterial flocculation, is under control of the tac operon. The plasmid-bearing cells are induced to flocculate in the separator, whereas cell growth and product synthesis occur in the main fermentation vessel without the inhibiting effects of pilin production. Selective recycle allows for the maintenance of the plasmid-bearing cells by separating flocculent, plasmid-bearing cells from nonflocculent, segregant cells in an inclined settler, and recycling only the plasmid-bearing cells to the reactor. As a result, product expression levels are maintained that are more than ten times the level achieved without selective recycle. All experimental data agree well with theoretical predictions.
    Additional Material: 10 Ill.
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