Publication Date:
2012-11-28
Description:
The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. The segmented negative-stranded RNAs are associated with the polymerase complex and nucleoprotein (NP), forming ribonucleoproteins (RNPs), which are responsible for virus transcription and replication. We describe the structure of native RNPs derived from virions. They show a double-helical conformation in which two NP strands of opposite polarity are associated with each other along the helix. Both strands are connected by a short loop at one end of the particle and interact with the polymerase complex at the other end. This structure will be relevant for unraveling the mechanisms of nuclear import of parental virus RNPs, their transcription and replication, and the encapsidation of progeny RNPs into virions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Arranz, Rocio -- Coloma, Rocio -- Chichon, Francisco Javier -- Conesa, Jose Javier -- Carrascosa, Jose L -- Valpuesta, Jose M -- Ortin, Juan -- Martin-Benito, Jaime -- New York, N.Y. -- Science. 2012 Dec 21;338(6114):1634-7. doi: 10.1126/science.1228172. Epub 2012 Nov 22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Macromolecular Structure, Centro Nacional de Biotecnologia [Consejo Superior de Investigaciones Cienficas (CSIC)], Madrid, Spain.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23180776" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Nucleus/metabolism/virology
;
Cryoelectron Microscopy
;
Electron Microscope Tomography
;
Image Processing, Computer-Assisted
;
Influenza A Virus, H1N1 Subtype/*chemistry/physiology/ultrastructure
;
Madin Darby Canine Kidney Cells
;
Microscopy, Electron
;
Models, Molecular
;
Protein Conformation
;
Protein Structure, Secondary
;
RNA Replicase/chemistry/metabolism/ultrastructure
;
RNA, Viral/*chemistry/metabolism
;
RNA-Binding Proteins/chemistry/metabolism/ultrastructure
;
Ribonucleoproteins/*chemistry/metabolism/ultrastructure
;
Transcription, Genetic
;
Viral Core Proteins/chemistry/metabolism/ultrastructure
;
Viral Proteins/*chemistry/metabolism/ultrastructure
;
Virion/*chemistry/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
