Publication Date:
2001-04-17
Description:
In mammals, the central circadian pacemaker resides in the hypothalamic suprachiasmatic nucleus (SCN), but circadian oscillators also exist in peripheral tissues. Here, using wild-type and cryptochrome (mCry)-deficient cell lines derived from mCry mutant mice, we show that the peripheral oscillator in cultured fibroblasts is identical to the oscillator in the SCN in (i) temporal expression profiles of all known clock genes, (ii) the phase of the various mRNA rhythms (i.e., antiphase oscillation of Bmal1 and mPer genes), (iii) the delay between maximum mRNA levels and appearance of nuclear mPER1 and mPER2 protein, (iv) the inability to produce oscillations in the absence of functional mCry genes, and (v) the control of period length by mCRY proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yagita, K -- Tamanini, F -- van Der Horst, G T -- Okamura, H -- New York, N.Y. -- Science. 2001 Apr 13;292(5515):278-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11303101" target="_blank"〉PubMed〈/a〉
Keywords:
ARNTL Transcription Factors
;
Animals
;
Basic Helix-Loop-Helix Transcription Factors
;
Biological Clocks/*genetics
;
CLOCK Proteins
;
Cell Cycle Proteins
;
Cell Line
;
Cell Nucleus/metabolism
;
Circadian Rhythm/*genetics
;
Cryptochromes
;
*DNA-Binding Proteins
;
*Drosophila Proteins
;
Endothelin-1/pharmacology
;
*Eye Proteins
;
Fibroblasts/*physiology
;
Flavoproteins/genetics/metabolism
;
Gene Expression Profiling
;
*Gene Expression Regulation
;
Nuclear Proteins/genetics/metabolism
;
Period Circadian Proteins
;
*Photoreceptor Cells, Invertebrate
;
RNA, Messenger/genetics/metabolism
;
Rats
;
Receptors, G-Protein-Coupled
;
Suprachiasmatic Nucleus/metabolism
;
Time Factors
;
Trans-Activators/genetics/metabolism
;
Transcription Factors/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
