Publication Date:
2001-03-17
Description:
DNA polymerase iota (pol iota) is one of several recently discovered DNA polymerases in mammalian cells whose function is unknown. We report here that human pol iota has an intrinsic 5'-deoxyribose phosphate (dRP) lyase activity. In reactions reconstituted with uracil-DNA glycosylase (UDG), apurinic/apyrimidinic (AP) endonuclease and DNA ligase I, pol iota can use its dRP lyase and polymerase activities to repair G*U and A*U pairs in DNA. These data and three distinct catalytic properties of pol iota implicate it in specialized forms of base excision repair (BER).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bebenek, K -- Tissier, A -- Frank, E G -- McDonald, J P -- Prasad, R -- Wilson, S H -- Woodgate, R -- Kunkel, T A -- New York, N.Y. -- Science. 2001 Mar 16;291(5511):2156-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Genetics and, Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11251121" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Pairing
;
Base Sequence
;
Carbon-Oxygen Lyases/metabolism
;
DNA/*metabolism
;
*DNA Glycosylases
;
DNA Ligases/metabolism
;
*DNA Repair
;
DNA-(Apurinic or Apyrimidinic Site) Lyase
;
DNA-Directed DNA Polymerase/chemistry/*metabolism
;
Deoxyribonuclease IV (Phage T4-Induced)
;
Humans
;
Molecular Sequence Data
;
N-Glycosyl Hydrolases/metabolism
;
Phosphorus-Oxygen Lyases/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Ribosemonophosphates/*metabolism
;
Schiff Bases
;
Uracil/metabolism
;
Uracil-DNA Glycosidase
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
