Publication Date:
2012-11-09
Description:
High-performance liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI–MS/MS) approaches have enabled high selectivity and sensitivity for the identification and quantification of glucosylceramide molecular species. Here we demonstrate that HPLC–ESI–MS/MS is an efficient method for characterizing plant glucosylceramide species having the cis -8 and trans -8 isomers of sphingoid bases. Complete baseline separation was achieved using a high-carbon-content octadecylsilyl column and a simple binary gradient comprising methanol and water. The result of 2-hydroxy fatty acid composition achieved by HPLC–ESI–MS/MS was compared with that achieved by gas chromatography with flame ionization detection (GC–FID), indicating that the two methods yield similar molar compositions. The current method should be applicable to seeking the active components of glucosylceramide species from plant materials in response to biological challenges. Content Type Journal Article Category Methods Pages 1-9 DOI 10.1007/s11745-012-3725-7 Authors Hiroyuki Imai, Department of Biology, Graduate School of Natural Science, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe, 658-8501 Japan Hideyasu Hattori, Development and Marketing Group, Frontier Science Business Division, Shiseido Co., Ltd., 12 Nishikawabe-cho, Higashikujyo, Minami-ku, Kyoto, 601-8037 Japan Masayuki Watanabe, Department of Biology, Graduate School of Natural Science, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe, 658-8501 Japan Journal Lipids Online ISSN 1558-9307 Print ISSN 0024-4201
Print ISSN:
0024-4201
Electronic ISSN:
1558-9307
Topics:
Biology
,
Chemistry and Pharmacology
