Publication Date:
2012-04-17
Description:
Sensitivity of baker’s yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demonstrated by increased superoxide production and decrease of cellular glutathione content. Anoxic atmosphere and hydrophilic antioxidants (ascorbate, glutathione, dithiothreitol, cysteine, and N -acetylcysteine) ameliorated DSF toxicity to yeast indicating that oxidative stress plays a critical role in the cellular action of DSF. Content Type Journal Article Category Original Research Pages 1-9 DOI 10.1007/s10565-011-9200-z Authors Magdalena Kwolek-Mirek, Department of Biochemistry and Cell Biology, University of Rzeszow, ul. Zelwerowicza 4, 35-601 Rzeszow, Poland Renata Zadrag-Tecza, Department of Biochemistry and Cell Biology, University of Rzeszow, ul. Zelwerowicza 4, 35-601 Rzeszow, Poland Grzegorz Bartosz, Department of Biochemistry and Cell Biology, University of Rzeszow, ul. Zelwerowicza 4, 35-601 Rzeszow, Poland Journal Cell Biology and Toxicology Online ISSN 1573-6822 Print ISSN 0742-2091 Journal Volume Volume 28 Journal Issue Volume 28, Number 1
Print ISSN:
0742-2091
Electronic ISSN:
1573-6822
Topics:
Biology
,
Medicine
