ISSN:
1089-7623
Source:
AIP Digital Archive
Topics:
Physics
,
Electrical Engineering, Measurement and Control Technology
Notes:
A stopped-flow apparatus was constructed for x-ray scattering study at subzero temperature. It can be operated over a wide temperature range down to −20 °C with highly viscous solution (20 cP) successfully. We have applied the stopped-flow x-ray scattering method to many biological reactions. In particular, the association of E. coli ribosomal subunits was detected at −10 °C which was too fast to be detected at room temperature. Dissociation of E. foetida hemoglobin was measured by the stopped-flow x-ray scattering method combined with a time-resolved imaging plate as a detector.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1063/1.1140768