ISSN:
1573-5001
Keywords:
Triple-resonance NMR
;
Quantitative J spectroscopy
;
3JC′Cγ and 3JNCγ scalar couplings
;
χ1 dihedral angle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Pulse sequences are presented for the measurement of3JC′Cγ and3JNCγ scalar couplings for allCγ containing residues in15N,13C uniformly labeled proteins. The methodsdescribed are based on quantitative J correlation spectroscopy pioneered byBax and co-workers [Bax et al. (1994) Methods Enzymol., 239, 79–105].The combination of 3JC′Cγ and3JNCγ scalar coupling constants allows theassignment of discrete rotameric states about the χ1 torsion angle in cases where such states exist or, alternatively,facilitates the establishment of noncanonical χ1conformations or the presence of rotameric averaging. The methods areapplied to a 1.5 mM sample of staphylococcal nuclease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018354712430
