Publication Date:
2001-11-10
Description:
Microtubules are dynamically unstable polymers that interconvert stochastically between polymerization and depolymerization. Compared with microtubules assembled from purified tubulin, microtubules in a physiological environment polymerize faster and transit more frequently between polymerization and depolymerization. These dynamic properties are essential for the functions of the microtubule cytoskeleton during diverse cellular processes. Here, we have reconstituted the essential features of physiological microtubule dynamics by mixing three purified components: tubulin; a microtubule-stabilizing protein, XMAP215; and a microtubule-destabilizing kinesin, XKCM1. This represents an essential first step in the reconstitution of complex microtubule dynamics-dependent processes, such as chromosome segregation, from purified components.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kinoshita, K -- Arnal, I -- Desai, A -- Drechsel, D N -- Hyman, A A -- New York, N.Y. -- Science. 2001 Nov 9;294(5545):1340-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307, Dresden, Germany. kinoshita@mpi-cbg.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11701928" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Biopolymers/metabolism
;
Kinesin/isolation & purification/*metabolism
;
Microscopy, Interference
;
Microtubule-Associated Proteins/isolation & purification/*metabolism
;
Microtubules/chemistry/*metabolism/ultrastructure
;
Recombinant Proteins/metabolism
;
Tubulin/isolation & purification/*metabolism
;
Xenopus
;
*Xenopus Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
