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  • 1
    Publication Date: 2021-05-19
    Description: Caspian trout (Salmo trutta caspius) is one of valuable fishes of the Caspian sea for people living in the Northern coastal area of Iran. Since fish stock and natural resources protection is one the Iranian fisheries research objectives, the most activity on Caspian trout rehabilitation was focused on releasing thousands of smolts in the rivers discharge to Caspian Sea. Catch statistics of this species has declined over last decades thus this study on quality of provided brood stocks and producing fries with suitable quality can help rehabilitation and rearing of this valuable species. Several rivers were considered for providing brooders to keep genetic resources variety of sea stocks. In spawning season 25 (9 male, 16 female) and 30 (5 male, 25 female) brooders were collected from fishermen in Mazandaran rivers and Gyilan coasts provinces respectively. After propagation and larvae rearing, 11500 (10-15 g.) and 50000 (less than 10 g.) juveniles were released in Gyilan and Mazandaran rivers respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Caspian trout ; Salmo trutta caspius ; Brood stocks ; Rehabilitation ; Fisheries ; Species ; Rearing ; Genetic ; Propagation ; Juvenile
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 32pp.
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  • 2
    Publication Date: 2021-05-19
    Description: In order to domesticate Litopenaeus vannamei as a solution to single species shrimp aquaculture of Penaeus indicus and to reduce risk of shrimp culture in emergency cases, this study was carried out. Initially the postlarvae reared from imported L. vannamei were cultured. After culture season the adult shrimps were transported to greenhouse ponds for wintering. It is then relocated to hatchery saloon. After eye stalk ablation and maturation the female brood stocks were introduced to matured male for copulation at ponds. During the study period, male and female shrimps attained full maturation at end – April by temperature over 25ºC, the copulation process was started significantly. Finally of the 12 instances of copulation, one instance resulted nauplii production. The best maturation was observed at 28 ֯C ±1 and salinity30 to 33PPT and pH of 8 to 8.3 Fresh sea worms and squid showed the highest effect on fecundity in brood stocks. Besides, the spring season was obtained to be the best period for brood stock production in Bushehr province.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; Brood stocks ; Larvae ; Species ; Shrimp ; Aquaculture ; Postlarvae ; L.vannamei ; Hatchery ; Maturation ; Nauplii ; pH ; Sea worm ; Squid
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 25pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecological ; Physico-chemical ; Vannamei shrimp ; Litopenaeus vannamei ; Water quality ; Abadan aquaculture site ; Rearing ; Shrimp ; Ponds ; Sampling ; Plankton ; Heavy metals ; pH ; Temperature ; Benthos ; Phytoplankton ; Zooplankton ; Copepods ; Nematoda larvae
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 80pp.
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  • 4
    Publication Date: 2021-05-19
    Description: The brine shrimp Artemia consists of a number of bisexual species and a large number of parthenogenetic forms, which collectively, inhabiting a wide range of hypersaline habitats. This research was carried out in order to study the genetic differentiation of Artemia urmiana and two parthenogenetic populations originated from surrounding lagoons of the Lake Urmia (Fesendoz, Saray village) and also to find the phylogenetic relationships of some Iranian Artemia (Qom, Arak, Golestan, and Shiraz) with a comparison to some foreign Artemia species (A. sinica, A. franciscana, A. parthenogenetica (Russia and Turkmenistan) using a molecular technique (PCRRAPD`s). Of a total number of 56 random primers (10 bp) survey, 29 and 26 oligonucleotides showed polymorphic patterns with highly reproducible bands in Artemia urmiana groups and Iranian Artemia groups.The highest percentage of polymorphic loci were in Artemia urmiana (P= 78.89 %) and in comparison with the other parthenogenetic forms, in parthenogenetic Artemia from Saray village (P= 59.8 %). The highest values of Shannon index were estimated in Artemia urmiana (I= 0.41), and in comparison with other parthenogenetic forms, in parthenogenetic Artemia from Saray village (I= 0.3). Analysis of molecular variance indicated that most variation were within populations (A. urmiana with parthenogenetic forms, FST= 0.35) and among populations (Iranian Artemia with foreign species, FST= 0.76). Genetic pattern was not in accordance to geographical distribution, which indicated the wide range of Artemia samples at different regions in Iran. UPGMA showed two distinct clusters in group I including A. urmiana with two parthenogenetic forms and four clusters in group II including Iranian Artemia with some foreign species. It is understood that two parthenogenetic populations (Fesendoz and Saray village) had close genetic relationship together with high value of heterozygosity in parthenogenetic Artemia from Saray village. Iranian Artemia (Qom, Arak, and Golestan) indicated close genetic relationships with A. parthenogenetica (Russia and Turkmenistan) with low values of heterozygosity. A sister group was generated including Shiraz and USA Artemia samples which attests a possible contamination of local water with A. franciscana. FST value revealed high genetic structuring at all studied groups. Besides, it is suggested that gene flow was not strong enough to prevent substantial genetic differentiation. Result could lead to a management programmes to use suitable Artemia strains with high genetic diversity, for culturing and establishment of gene bank of Artemia stocks in Lake Urmia.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Artemia urmiana ; Parthenogenetic populations ; Iranian Artemia ; Phylogenetic relationships ; RAPD`s ; Shrimp ; Bisexual species ; Phylogenetic ; A. sinica ; A. franciscana ; A. parthenogenetica ; Survey ; A. urmiana
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 5
    Publication Date: 2021-05-19
    Description: This study investigates the distribution of three trace elements (Cd, Cu and Zn) in tissues (exoskeleton, abdominal muscle and hepatopancreas) of two shrimp species (Penaeus merguiensis and Penaeus semisulcatus). Special emphasis in this study was placed on evaluating the effects of storage conditions on redistribution of the elements and moreover, the potential roles of metallothionein in tissue redistribution. The specimens were collected from the northern part of Persian Gulf during August 2001 and September 2002. The concentrations of metals in the tissues were measured using an Inductively Coupled Plasma-Atomic Emission Spectrophotometer (ICP-AES) and an ICP-Mass Spectrometer (ICP-MS). Metallothionein levels were determined using Differential Pulse Polarography. Results show a similar hierarchy for Cd and Cu concentrations among different tissues as follows: hepatopancreas〉 exoskeleton〉 muscle; and for Zn, the hepatopancreas again contained the highest levels, though there were not any significant differences in Zn concentrations between muscle and exoskeleton. Significant differences were found between the species in the case of Cd and Zn concentrations. Cd and Zn levels in P. semisulcatus were markedly higher than in P. merguiensis. In general, there were highly significant differences among the storage conditions from accumulation the selected trace elements and metallothionein in both species point of view, but in most cases no significant differences could be observed among the fresh, refrigerated and frozen specimens of P. semisulcatus. In regards to Zn and Cu accumulation in edible (muscle) tissues of P. merguiensis, optimum conditions for frozen storage is -30ºC as ice frozen, and in terms of short term storage, one day refrigeration is recommended. The redistribution of Zn and Cu during the storage period can be attributed to the role of metallothionein in their transport from organ(s) to muscle during storage. The variations in pH values measured in the muscle of P. merguiensis in different storage conditions showed a similar trend with metallothionein, Zn and Cu. In contrast it appears that cadmium is transported from muscle to the other tissues. Exoskeleton is probably one of the recipient tissues of cadmium. The inter-elemental relationships were assessed for each species, separately. A significant positive relationship was observed between the concentrations of Zn and metallothionein in P. semisulcatus, whereas in P. merguiensis, Cd concentrations decreased significantly with increasing metallothionein. Highly significant differences between the sexes in Cu and Zn contents could be detected. Highly significant size-related differences were found in the levels of Cd, Cu and metallothionein in the selected tissues of the shrimp species. Comparison between the mean concentrations of the elements in muscle tissue and existing guidelines indicate that in nearly all cases the concentrations were well below permissible levels for human consumption. The only exception was observed regarding mean values of Cd, which was somewhat greater than some of the recommended levels.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Heavy metals ; Distribution ; Redistribution ; Shrimp ; Metallothionein ; Storage conditions ; Tissues
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 50pp.
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  • 6
    Publication Date: 2021-05-19
    Description: An artificial Reef is a fabricated, underwater structure, typically built for promoting marine life in areas of generally featureless bottom. Creating artificial reefs began from 2000 in southern provinces in Iran. This study has surveyed about the Bushehr and Khozestan states. The objective is to determine the effectiveness of artificial reefs in attracting and enhancing the production different aquatics through increasing of fish assemblage and fishery. The performance of artificial reefs deployed off the coast of the Persian Gulf in 10 to 15m, was evaluated. The reefs comprised of seven artificial and one control (natural) statistical tests plan in three replicates. Three types of artificial reefs were used in this study. That two were designed reefs and one was not designed or used material. The experimental plan consisted (i) Reef ball (R), designed hemispherical shaped; (ii) Laneh Mahi (L), designed pyramid shaped; (iii) Used material (U); (iv) mixed (RL); (v) mixed (RU); (vi) mixed (LU); (vii) mixed (RLU); and (viii) control site (CS). Trap nets and under-water visual census surveys were adopted for seasonal sampling of fish aggregation. Results of statistical analysis using ANOVA and T-test of the mean Catch per Unit Effort (CPUE) showed significant difference (p〈0.05) in term of computing number of fish for aggregation of fishes. The study has concluded that reef deployments have influenced favorably the fish communities and fish harvests. Therefore, the artificial reefs, especially the mixed RLU, are appropriate tools for future fishing enhancement in the Persian Gulf of Iran.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Artificial reefs ; Fisheries ; Marine ; Survey ; Hemispherical ; ANOVA ; CPUE ; Underwater structure
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 188pp.
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  • 7
    Publication Date: 2021-05-19
    Description: Protecting coastal aquaculture systems as a habitat for aquatic systems is one of the serious concerns of the world. In the present study, spatial and temporal variations of Some physicochemical parameters, nutrient concentration and water quality In some estuaries of East and West of Hormozgan province were investigated from March 2015 to February 2016.Data analysis was performed using multivariate statistical methods (such as: CA,DA and PCA Analysis) and Eutrophication and water quality index using multivariable indices. The average annual variation of various parameters studied in West Azarbaijan province of Hormozgan is as follows:water temperature (18.1-34.6Co ), Salinity (35-45.6 ppt), Dissolved Oxygen (4.6-7.4 mg.l), pH (8.01-8.4), Nitrate (32.5- 403.5µmo.l), Nitrite (3.6-50.7 µmo.l), Total Ammonia (9.8-311.2 µmo.l), phosphate (22-569.1.6 µmo.l), and chlorophyll-a is equal to(0.46-4.04 mg.m3 ), respectively.Also, the mean annual variation of the various parameters studied in the eastern province of Hormozgan has been as follows: water temperature (18.1-34.6Co ), Salinity (35-45.6 ppt), Dissolved Oxygen (4.6-7.4 mg.l), pH (8.01-8.4), Nitrate (32.5-403.5µmo.l), Nitrite (3.6- 50.7 µmo.l), Total Ammonia (9.8-311.2 µmo.l), phosphate (22-569.1.6 µmo.l), and chlorophyll-a is equal to(0.46-4.04 mg.m3 ), respectively. One-Way Analysis of Variance test showed that the significant for some differences physicochemical parameters varied seasonally.Discriminant analysis, water quality conditions in terms of location classified into three groups.The analysis of PCA showed that the amount of nitrate and total ammonia in the tiba and phosphate in Laft and Kgamir Stricks with two sources (domestic and agricultural waste) and natural sources, can be considered as the main factors affecting the intensity of Eutrofication.The range of trophic Trix and TI eutrophication index in the studied areas were 4.3-6.1 and 3.5-4.2 respectively.According to on the indicators of and TI index, trophic status was classified into mesotrophic(in laft and khamir areas), Oligo-Mezotrophic(Yekshoveh area) and mezo- eutrophic respectively. Also Based on Trix index, the Water quality status was from bad condition in tiab area and good condition in laft, khamir and yekshoveh areas respectively. The determination of trophic conditions with a single-variable method based on chlorophyll a and Total inorganic Dissolved materials(DIN) showed a Very good agreement with multivariate trophic indices.The range of changes in some of the parameters studied in this study was more than the reported limit for the conservation of aquatic life in coastal areas.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Shrimp ; Habitats
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 76pp.
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  • 8
    Publication Date: 2021-05-19
    Description: Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal conservation especially for endangered species. Ideally, barcodes could be used to positively identify illegally obtained material even in cases where diagnostic features have been purposefully removed or to release confiscated organisms into the proper breeding population. In the present study molecular data were used besides the morphological ones to introduce a specific barcode for six selected species of Shrimp. Based on the previous phylogenetics study, it has been proved that the mitochondrial COI gene in crustacean is a good discriminative marker at both inter- and intra-specific levels. In this regard, six species of shrimp (Fenoro penaeus indicus, Fenoro penaeus merguensis, penaeus semisulcatus, Metapenaeus affinis, Marsupenaeus japonicas, Fenoro penaeus penicillatus were collected from the Persian Gulf and Oman sea. After identification, the total DNA was extracted; COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. Molecular analysis revealed some degree of interpopulation differences but six good species were recognizable based on COI sequences. Also for population study molecular data of two species Penaeus semisulcatus and Metapenaeus affinis were analysed base on COI RFLP and 16SrRNA sequences respectively. The results would be helpful to protect shrimp species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Morphological ; Population ; Genetic ; Shrimp ; Species ; DNA barcoding ; Partial sequencing ; mtDNA ; Molecular method ; PCR ; Fenoro penaeus indicus ; Fenoro penaeus merguensis ; Penaeus Semisulcatus ; Metapenaeus affinis ; Marsupenaeus japonicas ; Fenoro penaeus penicillatus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 74pp.
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  • 9
    Publication Date: 2021-05-19
    Description: The effects of added artificial substrates on juvenile white Indian shrimps (Penaeus indicus) were evaluated. .....
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Aquaculture ; Juveniles ; Feed conversation ratio
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 61pp.
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  • 10
    Publication Date: 2021-05-19
    Description: The shrimp farming success in the future will greatly depend upon increasing supplies of healthy and high quality seed for stocking ponds. The limited and inconsistent availability of wild shrimp postlarvae together with an urgent need of establishing selective breeding programs, increased the interest in captive broods of penaeid. Many investigations have been carried out on several shrimp species world-wide. This study have been conducted on 2 shrimp groups since September (Mehr) to July (Tir) 1999 in Bandar-e-Imam southwest Iran. The wild group (SPB) prepared from Jask area and the pond group (POB) prepared from artificial ponds upon harvesting them as marketable size have been investigated. 2 groups were exposed to natural diets consisting of squid and artemia biomass in same conditions using 12 m3 concrete tanks. Stocking density was 2 piece/m2 (1F:1M). Half part of the pond bottom covered by fine sand to reduce the stress where the other part has been used for feeding. Daily ration given in 3 times by satiation method. Also reproductive performance of wild gravid brooders (WLB) were recorded as a control group. 2 examined groups well grown and around 50% of the SPM and around 30% of POB females successfully ripened a produced eggs and nauplii where almost all males of both groups were matured. Data showed fecundity is highly correlated to the TL and W of the brooders by positive trend. Considering the TL, W, Fecundity and nauplii production the WLB brooders are significantly (P=0.05) higher than 2 other groups, while the SPM broods are significantly (P=0.05) higher in hatchability. Meanwhile the survival rate from nauplii to postlarvae stage was around 30% for SMP brooders. On the other hand the growth parameters (TL, W) of the marketable size of the shrimps produced from SMP brooders were significantly (P=0.05) higher than those produced from WLB group. Since the fecundity is improvable by increasing the brood size thus the data suggest the sup.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Fatty acids ; Penaeus indicus ; Brood ; Juvenile ; Shrimp ; Postlarvae ; Species ; Squid ; Artemia ; Biomass ; Nauplii ; Fecundity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 42pp.
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