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  • 1
    Publication Date: 2021-05-19
    Description: Caspian trout (Salmo trutta caspius) is one of valuable fishes of the Caspian sea for people living in the Northern coastal area of Iran. Since fish stock and natural resources protection is one the Iranian fisheries research objectives, the most activity on Caspian trout rehabilitation was focused on releasing thousands of smolts in the rivers discharge to Caspian Sea. Catch statistics of this species has declined over last decades thus this study on quality of provided brood stocks and producing fries with suitable quality can help rehabilitation and rearing of this valuable species. Several rivers were considered for providing brooders to keep genetic resources variety of sea stocks. In spawning season 25 (9 male, 16 female) and 30 (5 male, 25 female) brooders were collected from fishermen in Mazandaran rivers and Gyilan coasts provinces respectively. After propagation and larvae rearing, 11500 (10-15 g.) and 50000 (less than 10 g.) juveniles were released in Gyilan and Mazandaran rivers respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Caspian trout ; Salmo trutta caspius ; Brood stocks ; Rehabilitation ; Fisheries ; Species ; Rearing ; Genetic ; Propagation ; Juvenile
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 32pp.
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  • 2
    Publication Date: 2021-05-19
    Description: Larvae of the chironomid midges (blood worms) are known as use full live food for cultured aquatics. There are endemic blood worms in sediments of the earthponds of Bafgh area at center of Iran. This study tended to identification of two chironomid species, Chironomus aprilinus Meigen, 1830 and Dicrotendipes sudanicus Freeman, 1959 & 1961, from Iran as a new record. Culture of blood worms was successful in glass thanks with 16h. light, at 24-26°C temperature. Eggs were incubated after 14 days. Suitable concentration of harvesting is 1-2 egg masses (1250eggs/m2). Enrichment of earthponds or plastis thanks including sedime is offered for culture of chironomid larvae. Collectors prepared with low cost materials are useful for collection of egg masses. May, June and July are the best time for egg collection. Large blood worms are mainly avalable in winter. Results of body analysis of the larvae indicated high dry matter with good quality.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biotechnic ; Chironomid ; Identification ; Culture ; Collector ; Brackish water ; Chironomidae ; Aquatic ; Larvae ; Blood worm ; Sediments ; Species ; Chironomus aprilinus ; Chironomus aprilinus ; Feed ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: Carp aquaculture is one of Maine potential for commercial and rural development , employing enhancement and earning income .This enterprise which is conducted by using fresh water and unfertile lands have been attended in the country since last seven decades. In accordance to wide area of cultivation in the country, maximum production is about 150000 tonnes in 2010. Establishment of aquaculture center by technical deputy of president, made a suitable opportunity for preparation of carp development road map. In this report, present condition and problems in front of carp development have been analyzed , and regarding to targets of long term country development, projects for research and executive sectors have been prepared.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Aquaculture ; Warm Water ; Fish ; Carp ; Development ; Road map ; Culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 115pp.
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  • 4
    Publication Date: 2021-05-19
    Description: An artificial Reef is a fabricated, underwater structure, typically built for promoting marine life in areas of generally featureless bottom. Creating artificial reefs began from 2000 in southern provinces in Iran. This study has surveyed about the Bushehr and Khozestan states. The objective is to determine the effectiveness of artificial reefs in attracting and enhancing the production different aquatics through increasing of fish assemblage and fishery. The performance of artificial reefs deployed off the coast of the Persian Gulf in 10 to 15m, was evaluated. The reefs comprised of seven artificial and one control (natural) statistical tests plan in three replicates. Three types of artificial reefs were used in this study. That two were designed reefs and one was not designed or used material. The experimental plan consisted (i) Reef ball (R), designed hemispherical shaped; (ii) Laneh Mahi (L), designed pyramid shaped; (iii) Used material (U); (iv) mixed (RL); (v) mixed (RU); (vi) mixed (LU); (vii) mixed (RLU); and (viii) control site (CS). Trap nets and under-water visual census surveys were adopted for seasonal sampling of fish aggregation. Results of statistical analysis using ANOVA and T-test of the mean Catch per Unit Effort (CPUE) showed significant difference (p〈0.05) in term of computing number of fish for aggregation of fishes. The study has concluded that reef deployments have influenced favorably the fish communities and fish harvests. Therefore, the artificial reefs, especially the mixed RLU, are appropriate tools for future fishing enhancement in the Persian Gulf of Iran.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Artificial reefs ; Fisheries ; Marine ; Survey ; Hemispherical ; ANOVA ; CPUE ; Underwater structure
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 188pp.
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  • 5
    Publication Date: 2021-05-19
    Description: Striped grey mullet (Mugil cephalus) is a marine fish with high commercial value and widespread distribution. The grey mullet and some other mullets can be found in saltwater, brackish and fershwater habitats at tropical, subtropical and also temporal climates.This species is suitable for aquaculture worldwide due to living in wide ranges of water temperatures and salinities, acceptable growth rate, suitable food conversion ratio, marketability, suitable species for polyculture with shrimps, marine fish and even with freshwater carps. The grey mullet is farming in different countries such as Italy, Northern Africa countries ( Egypt), occupied Palestine, India, Pakistan, Japan, Hong Kong, Taiwan, Vietnam, Indonesia, coastal countries of southern pacific ocean and Hawaii. Ten thousands of fries of this species (average weight and length of 280 mg and 28.3 mm respectively) imported in March 2008 from Egypt. The wild fries colleted from surrounding natural waters of Alexandria. After finishing the quarantine steps, they stocked in four earthern ponds (each with 2500 m2) located at the Gomishan shrimp educational centre at late march in 2008. At the first year of culture the densities were 5000 and 10000 individuals per hectar and each treatment with two replicates. At second years densities changed with 2000 and 2500 ind./hec. and each of traetments divided fishes with average primary weights of 80 and 115 grames. The fries and fish feeding begins in April when the water temperature rise above 18 °C and it cut in fall when the temperature goes under 20°C. They fed twice a day at 8 AM and 2 PM and the amount was 5-7 percents of the fish existent biomass in each ponds. They fed with dry formulated feed made of Mahdaneh Karadj Company (Danso dry feed) both the first and second years of farming. The feed prepared for carps feeding and containded 23±2 % crude protein, 10 ±2 % crude lipid, about 7 % crude fiber, 15 % ash, 40 ±2% carbohydrates and with energy content of 4000±200 kcal/kg. After seven months culture period the average weight and length of fish in treatment 5000 ind./hec. were 113.7 gr and 21.1 cm respectively and in treatment 10000 ind./hec. the average weight and length were 86.6 gr and 19.6 cm respectively. At first year the average survival rate in different treatments was 77 percent. The average FCR in treatments 5000 and 10000 ind./hec. were 3.4 and 3.9 respectively. At second years after six months culture the average weights in pond no. 9 (with primary weight 115 gr and density 2000 ind./hec) , pond no. 10 (with primary weight 80 gr and density 2000 ind./hec) , pond no. 11 (with primary weight 115 gr and density 2500 ind./hec) and pond no. 12 (with primary weight 80 gr and density 2500 ind./hec) calculated as 476.6 ,338.1, 366.5 and 440 gr respectively. The average survival rate and FCR were 93 % and 3.1 respectively. Ponds no. 9 and 11 (fish stocked with primary weight 115 gr in densities both 2000 and 2500 ind./hec) selected as the best economic efficient treatments.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Grey mullet ; Cultivation ; Economic efficiency ; Mugil cephalus ; Culture ; Marine ; Saltwater ; Brackishwater ; Fershwater ; Species ; Aquaculture ; Salinity ; Survival rate
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 81pp.
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  • 6
    Publication Date: 2021-05-19
    Description: This investigation conducted for evaluation Silo (Cylindrical) Fish Tanks as an applicable method for aquaculture of Rainbow trout (Oncorhynchus mykiss). Current research was designed in two practical phases as accidental experiments through 3 treatments and repeats of which stocked by three Rainbow trout (375 gr) densities as 75, 100 and 125 fish/m3 in 9 silo fish tanks (2 m3). For better understandings of the silo performance method, concrete circular fish tanks (similar volume) considered as controls and stocked as similar fish densities as silo tanks. The duration of the first phase of experiment was 150 days. The second phase of the experiment was determined for evaluating two proposed silo type (Inlet and outlet in bottom side / Inlet from bottom and outlet in upper side). In this stage, proposed silos (stocked by fish of 294 gr weigh) tested for rearing trout for 30 days. Water supplied from two surface wells after some aeration operations. Stocked fish were fed by pellets made by Fish Feed Chineh Company (Ltd) in Iran. Feeding operation conducted according to the recognized manuals by consideration of water temperature, fish weight and biomass. Meanwhile, biometry operation conducted in 10 days intervals while fish were anaesthetized. In addition, water quality was determined by daily measurements. According to the results of the first phase of experiment, there were significant variations on Growth rate, survival rate, FCR, FCE and SGR through the cultured organisms in silo tanks compared to the circular tanks. Meanwhile, according the results of an economic study on this survey, the fish culture in silo tanks showed significant economical and profitable in compare to controls. In additions, the second phase of experiment showed better aeration conditions in silo tanks when inlet water supply from bottom side and outlet located in upper side. Because of the fine distribution of fish in silo tanks and more usage of depth by this method, fish culture would be an applicable and profitable technique in narrow lands and slopes through the mountains area. The maximum stocking rate recorded more than 100 Kg fish/m3 in silo tanks.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Practical ; Silo ; Cylindrical ; Aquaculture ; Rainbow trout ; Oncorhynchus mykiss ; Fish ; Feeding ; Temperature ; Weight ; Biomass ; FCR ; FCE ; SGR ; Culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 73pp.
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  • 7
    Publication Date: 2021-05-19
    Description: Gillan , Mazandaran and Gorgan provinces have temperate climate, thus they have more potential for aquatic animal culture. There are thousands hectare of lands for Aquaculure and fisheries in adjacent to Caspian Sea in this provinces. these areas(North alborz) have provided a favorable back ground for aquatic animal breeding and stock enhancement of species in the sea. As a result, this investigation executed during two stages (phases). At first stage, the goal of this project included the feasibility study and demonstrating the existing situation of fish stock enhancement activities in North Alborz area and second phase is to survey on distribution, and production activities of stock enhancing hatcheries as well as their constructions,and production time table. Survey was started frome 2006 to 2008 by using qestionaries in 7 hatcheries from tree provinces.results revieled that there are more than 10 species was subjected to restocking .Total releasing was about 300 millions of fry and finger lings in to the Caspian sea.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Stock Enhancement ; Broodstock ; Production ; Yield ; Artificial propagation ; Bony fishes ; Sturgeon fishes ; Restocking ; Aquatic ; Culture ; Aquaculure ; Fisheries ; Breeding ; Survey ; Hatcheries ; Fry ; Fingerlings
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 191pp.
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  • 8
    Publication Date: 2021-05-19
    Description: Thirty to 40% of total fish catch is converted to waste. Using different methods of hydrolysis of the protein can be recovered of fish waste and increase the amount of protein efficiency. In this study, the four enzymes Alcalase , protamex , pepsin and trypsin were used for hydrolysis of four fish species including common carp (Cyprinus carpio) , silver carp (Hypophthalmichthys molitrix) , grass carp (Ctenopharyngodon idella) and the Big head (Hypophthalmichthys nobilis). The effects of pH, temperature and hydrolysis time on the rate of hydrolysis were studied on soluble proteins and degree of hydrolysis (phase I ). In the second step, proximate factors of peptone been evaluated and eventually replace commercial peptone media MRS ( Lactobacillus plantarum , Lactobacillus delberuki , Lactobacillus casei , Streptococcus thermophilus ) and TSB ( Listeria monocytogenes, two species of Bacillus and Pseudomonas, Streptococcus faecium) and the optical density of bacteria at different times were compared with control samples . Results showed that the highest degree of hydrolysis and soluble proteins were referred to alcalase and protamex, pepsin and trypsin respectively. The highest value of hydrolysis, in all treatments, was attributed to grass carp and silver carp, common carp and big head respectively. The best pH and temperature for alcalase, protamex, pepsin and trypsin 8.5and 55, 7.5 and 55, 3.5, 37, 7 and 37 respectively. Best time to achieve the highest degree of hydrolysis and soluble protein was 90 minutes. Qualitative analysis showed that the highest and lowest amounts of protein and fat in the treatment of alcalase (about 70 % protein and less than 0.5 % fat ) and protamex, pepsin and trypsin was then . The results of bacteria culture showed that the highest percentage growth of lactic acid bacteria was referred to Streptococcus thermophilus and Lactobacillus casei had the lowest rate of growth. In other bacteria, Pseudomonas and Bacillus species were the highest percentage of growth and Listeria monocytogenes and Streptococcus faecium respectively. In all treatments, alcalase had the best results and the peptone prepared from fish waste grass carp had the best condition for growth of used bacteria. It seems that the initial substrate , the parameters used such as temperature, pH, and enzyme hydrolysis time , have a significant effect on the quality of peptone and protein content in the final product is determined value of protein for culture of bacteria.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Warm water ; Protease enzymes ; Bacteria ; Degree of hydrolysis ; Soluble proteins ; Fish waste ; Peptone ; Marine ; Culture ; Alcalase ; Protamex ; Pepsin ; Common carp ; Cyprinus carpio ; Silver Carp ; Hypophthalmichthys molitrix ; Grass carp ; Ctenopharyngodon idella ; Big head ; Hypophthalmichthys nobilis
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 92pp.
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  • 9
    Publication Date: 2021-05-19
    Description: Tilapia is the second cultured fish in the world. Up to 135 countries produces tilapia. Intensive culture systems of tilapia such as cage culture are recently developed because of limitations of water resources. In Iran, studies about tilapia were started in National Research Center of Saline Water Aquatics from November 2008 and some aspects of tilapia aquaculture and culture systems are surveyed. Investigation about cage culture of Nile tilapia, Oreochromis niloticus was performed in two culture periods of 2012 and 2014 in fresh and brackish water conditions of Iran for the first time. Stocking densities of fish in the cages were 25, 50, 75, 100, 125 and 150 fish/m^3 . 100g fish stocked reached 272-331 g during 80 days in the first study. However, 50g fish stocked reached 329-450 g during 145 days in the second study. Results of cage culture of tilapia in fresh and brackish water conditions showed ranges of FCR: 1.56-3.72 and 1.08-1.87, DGR: 1.63-2.91 and 2.14-2.92 g/day, SGR: 0.63-1.12 and 1.29-1.54, production: 5.98-38 and 7.17-41.6 kg/m^3 , respectively. Stocking densities 75-125 fish/ m^3 were appropriate especially in fish reared in brackish water cages.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Tilapia ; Cage ; Culture ; Density ; Oreochromis niloticus ; Nile tilapia ; Agriculture ponds
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 44pp.
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  • 10
    Publication Date: 2021-05-19
    Description: In order to study the Inheritance of Microsatellite Markers in the hybrid of Chalcalburnus chalcoides and Vimba vimba persa in the Southern part of the Caspian Sea, hybridization between the species was carried out during spring (2009) in Shahid Ansari reproduction and culture workstation. 30 samples from the produced larva and also small piece of dorsal and pectoral fin of their parents were collected and stored in pure ethanol (96%) and transferred to the biotechnology of the Caspian Ecology Academy. DNA was extracted from fin samples of the parents, whilst the whole F1 progeny was used to isolate the DNA by Phenol-chloroform method. Quantity and quality of extracted DNA was determined using 1% Agarose electrophorus. DNA was used for PCR ampilification of 10 microsatellite loci. The amplifications were run on 6% Polyacrylamid electrophorus. The present study showed that 7 out of 10 markers were polymorphic and variated between the parents. Goodness of fit to the Mendelian inheritance ratio was determined by X2 analysis. 4 primers were segregrated according to Mendelian expectations at a significance level of P 0.05. Based on UPGMA dendrogram (Nei, 1978) two different clusters were obtained in which Chalcalburnus chalcoides and hybrid were in the same cluster but Vimba vimba persa was in the other. It seems that Vimba vimba persa is a joint ancestor for these two. High heterozygosity presence in parents and their F1 shows the high potential of genetic variety in Chalcalburnus chalcoides and Vimba vimba persa which from the aquaculture management and conservation view is important and considering the high genetic variety in their hybrid, the hybrid has the sufficient potential for race improvement.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecology ; Chalcalburnus chalcoides ; Vimba vimba persa ; Hybrid ; Microsatellite ; Hybridization ; Culture ; Samples ; Larvae ; DNA ; Inheritance ratio ; Heterozygosity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 85pp.
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