ALBERT

Description: The determination and identification of plankton species in the Anzali lagoon was Carried out through a period of two years in 27 stations in the Shejan, Siakishim , Abkenar , Hendekhaleh andmain outlets of this lagoon from 1997 to 1999. The results of this study reveal that there are Totally 6 phyla of Phytoplankton that include , 134genera and 278 species of which 56 genera and 107 species belong to Phylum Chlorophyta , 39 genera and 87 species belong to Phylum Bacillariophyta ( Diatoms ) , 2 genera and 2 species belong to Phylum Xanthophyta , 4 genera and 4 species belong to Phylum Chrysophyta , 6 genera and 34 species belong to Phylum Euglenophyta , 8 genera and 11 species belong to Phylum Pyrrophyta , 18 genera and 32 species belong to Cyanophyta and one genus belongs to phylum Rhodophyta. It is evident from these results that Cyanophyta and Bacillariophyta showed the highest density among Phytoplanktons with the highest Aboundance found in the Abkenar and southern Hendekhalehregions . Highest density of Cyanophyta was observed in Abkenar region during the end of spring and beginnings of summer . Bacillariophyta was observed all round the year throughout the Anzali lagoon being more aboundant the end of Autumn, Winter and Spring . Other phyla showed similar population densities throughout the year . study of Zooplanktons revealed the presence 11 phylum that include 133 genera and 211 species Anzali lagoon of which 10 genera 22 species belong to phylum Rhizopoda ,4 genera 4 species belongto Phylum Actinopoda , 32 genera 41 species belong to Phylum Ciliophora , one genus and one specie belong to Phylum Porifera , 3 genera belong to Phylum Coelantrata , 2 genera and 2 species belong toAnnelida , 2 genera 2 species belong to Phylum Platyhelminthes , 36 genera and 90 species belong toPhylum Rotatoria , one genus and one specie belongs to Phylum Tardigrada , 19 genera and 32 species belong to Phylum Arthropoda (order Cladocera), 7 genera and 11 Species belong to Class Copepoda ,in Class Cirripedia and Ostracoda and Diptera(Family Chironomidae) each of them consisting of onegenus , 2 genera and 2 species belong to Class Archnida and in phylum Mollusca Class Bivalvia one genus was identified . It is evident from the present study that Rotrtoria was dominant in southern Hendekhaleh and Abkenar regions and the main outlets wherease Protozoa showed the maximum density in Shejan and Siakishem regions . Highest density of Zooplankton were observed in Hendekhaleh and Abkenar regions in end of Spring and during summer whereas all regions showed similar densities during the other seasons .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Regarding to monitor of demersal resources in the Persian Gulf and Oman Sea, and also biomass and CPUA estimation of them, a series of research cruises (5 cruises per year) were carried out by R/V Ferdows-1covering the area from 49 00 E in the west (Khoozestan provice) to 61 25 E in the east (Gwater) in a 5 years program form 2004 up to 2008 (no cruise in 2006). The study area was stratified into 17 strata (A to Q) of which 10 starta (A to J) were in the Persian Gulf and 7 strata (K to Q) were in the Oman Sea, covering the depths of 10-50m in the Persian Gulf and 10-100m in the Oman Sea. A total of 316 stations were randomly selected. The biomass and CPUA were estimated by Swept Area method. The comparison between two regions indicated that the percentage of density of demersal fishes in the Persian Gulf during years 2004, 2005, 2007 and 2008 were 3.3, 3.9, 2.3 and 2.4 times more than the Oman Sea and totally 70-80% of total biomass was calculated for the Persian Gulf. Also a comparison among 17 starta, the highest biomass was found for starta C & D in the Bushehr province waters. The amount of CPUA for both regions of the Persian Gulf and Oman Sea was compared and it was concluded that in years 2004 and 2005, the mean CPUA in the Persian Gulf was partially higher than the Oman Sea with 1.06 & 1.20 times more; and on the contrary for the next two years this value was higher in the Oman Sea with 1.3 times more. The highest CPUA of demersal resources for the Oman Sea belonged to the strata K & O for years 2004, 2007 and 2008 and for year 2005 was for L and strata. It can be concluded that the north-west of Oman Sea has the best condition of biomass of commercial and non-commercial species and the O stratum will be in the second ranking. With review the mean CPUA in different depth-layers for years 2004, 2005, 2007 and 2008, it was concluded that with increasing the depth, the mean CPUA is decreased in which the CPUA values in depth-layer 10-20m for these years were 8.3, 3.0, 2.1 & 1.6 times more than depth-layer 50-100m. The comparison of mean CPUA for 10 strata of the Persian Gulf showed that in years 2004 and 2005, the mean CPUA of demersal fishes in the Hormuzgan province waters was higher than in Bushehr province waters (1.2 times more). In total, the highest maen CPUA belonged to F (Naiband to Mogham) and G (Mogham to Farour) in Hormuzgan waters. On the contrary, in years 2007 and 2008 this value in Bushehr waters was 1.3 times higher than western part of Hormuzgan waters with the highest value in D stratum(Boordekhoon to Dayyer). Also the comparison of mean CPUA for different depth-layers, it was found that the mean CPUA has ascending trend with increasing the depth, with the highest value in depth-laer of 30-50m in which contains the 55-68% of total biomass. The lowest biomass is found in depth-layers of 10-30m. Totally, the minimum CPUA and biomass for both commercial and non-commercial species belonged to a stratum located in Khouzestan province waters and it was shown the over-exploitation of resources in this area. The comparison between commercial and non-commercial groups in both ecosystems , it was concluded that the density of commercial species was higher than non-commercial ones , and in all years the Persian Gulf indicated higher values than the Oman Sea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. In this library-based study the current situation of bony fish fisheries and biology of these species has been surveyed in Iranian waters of the Caspian Sea . In this survey internal and external environmental factors of bony fishes activities were considered. Using SWOT analysis method, current situation of fisheries was investigated..One of the most important strategies for conserving anadromous species such as Caspian kutum , common carp, roach , bream and …. is rehabilitation of the natural spawning ground in rivers, coastal lagoon specially Anzali lagoon .By conducting this strategy not only restocks of bony fishes is done ,but also reduced the cost of artificial restocking programs and releasing fingerlings .In short term , it is nessesary to enhance the stocks of the species that have low catch is done by huge releasing of the fingerlings and study the qualification of the program.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: According to the white spot disease during 2002 until 2005 and the damages caused by the disease to the farmers a new species L.vannamei were introduced to the farming region of Abadan Choebdeh from 2006. Hight mortality occurred in Khuzestan province farms in 2007 and 2008 again. Probably viral agent was transferred to native shrimps or other aquatic animals, therefore it is very important that presence of viruses in local aquatic animals and its vectors detected. Detection of WSSV, TSVand IHHNV in aquatic animal from Khuzestan costal region imphasis to wild shrimp and craps is the main objectives of this study. So samples were taken from 10 active farms twice a week and 100 postlarves from 3 active breeding center befor stocking.Samples for viral detection were studied by molecular and histopathology asseys. Results was shown presence of the White spot virus in cultured and wild shrimp and TSV infection in only two cultured shrimps but there was negative result for IHHN virus. To study the pathogenesis of white spot virus isolated from wild and farmed shrimps, exposure method was used. Results of challenge showed that WSSV can be transmited from diseased cultured and wild shrimp to cultured shrimp. Mean mortality percent in the treatment that fed with infected wild shrimp was 38.33± 10.4 and treatment that fed with infected cultured shrimp 61.66 ± 7.63 and in the positive control treatment was 87.5 ±3.5.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: From June 2004 to March 2006, a survey in Iran waters (Bushehr province) has completed. Sampling was accomplished in two area, north and south. In this period 15 cruises achieved in summer and 3 cruises in autumn and winter. In each cruise of north and south area 18 and 30 stations were sampled respectively. Stations row on three depth stratums that is, below 10 m, 10-20 m, and 20-30 m in total area from Bahrekan to Motaf. Some Biological parameters such as sex stages, sex ratio, mean total length, species composition were measured. Duration of shrimp fishing season in years 2004, 2005 and 2006 were 40, 44 and 40 days in summer respectively. In June and august the Catch per Unit Effort (CPUE) in south was more than northern area. CPUE increases in north area especially at the end of summer during fishing season gradually. Computing on Biomass estimation resulted 674, 1024 and 873 ton in years 2004, 2005 and 2006 respectively for all shrimp species (usually tiger shrimp, white shrimp and other). Total commercial landing of shrimp catch in above years were 1602, 1597 and 1110 ton respectively. Percentage of tiger shrimp in commercial landing in above years was 77, 75 and 84 respectively. We found that the least mean length of tiger shrimp was observed in southern area especially from Motaf to Rodmond stations. The schools of prawn penaeus semisulcatus enter to Bushehr waters from southern area that is Motaf region. Other than Motaf; Nakhilu, Rase khan and Rod-mond show same characteristics. Also in this region, density of shrimp increases with depth in most summer cruises. The result from analyses of gonad maturation studies shows the adult and mature females (stages III and IV) of P. semisulcatus accumulate in northern area at the end of autumn. This stock catches in autumn by illegal fishing.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The phytoplankton, zooplankton, Macrobenthos and ctenophore Mnemiopsis leidyi with Physico-chemical parameters and pollutants of water were investigated in the Caspian Sea during 2007-2008. The sample station located between 48°to° 54° longitude and 36° to 39° latitude. The samples were gathered in four seasons and eight transects (half-line perpendicular to the coast) in Astara, Anzali, Sefidrud, Tonekabon, Noshahr, Babolsar, Amirabad and Turkmen. Sampling was done of 5, 10, 20, 50 and 100M depth of different layers. Nonparametric test and multivariate analysis (PCA) were used for statistical methods. Physico-chemical factors: The results showed that the changes of physico-chemical factors, includes: water temperature, salinity, organic nitrogen and inorganic nitrogen (ammonium) are most important than other factors. The lowest sea surface water temperature was recorded in winter (7.2 oC) and highest in summer (29.8 oC) (p〈0.05). However, there was not significant difference at 50 and 100m depths in different seasons (p〉0.05). The lowest salinity was recorded in winter (10.42±0.14ppt) (p〈0.05) and the rest of the seasons, there was not significant difference (p〉0.05). The most inorganic nitrogen was determined (ammonium ion: 40.8±2 µg.l-1) in winter and the highest organic nitrogen (660.6±92 µg.l-1) in summer (p〈0.05). The concentration of inorganic nitrogen was evident from the surface layer to a depth of 100 meters (p〈0.05), but this trend was not observed in inorganic nitrogen (p〉0.05).The maximum concentrations of total nitrogen were measured at the near-shore stations (640.6±28 µg.l-1). The annual average water transparency was 4.91±0.24 m and increased in away from the beach (p〈0.05). Phytoplankton: in total, 191 species of phytoplankton were identified. The phylum of phytoplankton include: Bacillariophyta with 97 species (50.8%), Chlorophyta with 28 species (14.7%), Pyrrophyta with 26 species (13.6%), Cyanophyta with 25 species (13.1%) and Euglenophyta with 15 species (7.9%). Bacillariophyta and Euglenaphyta were had the most (51.49%) and the lowest (0.39%) abundance respectively. Nevertheless, Pyrrophyta and Chlorophyta were having the most (53.14%) and the lowest (0.54%) biomass respectively. Mean abundance and biomass of phytoplankton in the photic and the aphotic layers were a significant difference (p〈0.05). The average abundance and biomass decreased with increasing depth (p〈0.05). The most abundant and biomass phytoplankton was determined in the photic layer. Abundance and biomass of phytoplankton in the different seasons have been significant differences (p〈0.05).The most abundance of phytoplankton was in winter, fall, summer and spring respectively (p〈0.05) and the most biomass of phytoplankton was in winter, fall, spring and summer respectively (p〈0.05). Zooplankton: Also, 14 species of zooplankton include: 2 species of Copepoda, 5 species of Rotatoria, 2 species of Protozoa, 3 species of Cladocera, 2 species of newborn and larvae of Balanus and larvae of Bivalvia from meroplankton group were identified. However, the dominant zooplankton population was divided into three groups of copepoda, Rotatoria and meroplankton. Mean abundance and biomass of zooplankton in different seasons and layers showed significant different (p〈0.05). The most abundance and biomass of them in winter and the photic layer was determined. The mean abundance of Rotifera, Cladocera and protozoa in different depth was having not significant differences (p〉0.05). However, there was significantly in other groups (p〈0.05). The most abundance of them was in photic layer. Ctenophore: Average abundance and biomass of Mnemiopsis leidyiwere recorded 63±7 ind.m-3 and 7.70±2.34 g.m-3, respectively. The maximum seasonal average of abundance was 150 ± 17 ind.m-3 while the maximum biomass was 26.59± 4.61 g.m-3 in summer (p〈0.05). The lowest seasonal average of M. leidyi abundance and biomass were in the winter (6±2 ind.m-3 and 0.07±0.02 g.m-3, respectively) (p〈0.05).The average annual abundance and biomass of ctenophore were not significant difference in the 8 transects (p〉0.05). The maximum of ctenophore abundance and biomass were belonged to the depth of low than 20m (p〈0.05). The maximum of ctenophore abundance and biomass were recorded in the 5m depth of Anzali with 639 ind.m-3 and 158 g.m-3, respectively. Results of biological and non-biological factors showed that significant changes between euphotic and aphotic layers (p〈0.05).There were the most seasonal changes in the euphotic layer. The results showed that the presence of ctenophore is highly related to the presence of the zooplankton and phytoplankton and some non-biological factors. The water temperature is a limiting factor in the ctenophore Fluctuation at the winter, because of food presence (phytoplankton, zooplankton and Nutrients), M. leidyi have had the lowest abundance. Macrobenthos: Three phyla of macrobenthos (arthropoda, annelida and mollusca) include: 5 classes (crustacea, insecta, polycheata, oligocheata and bivalvia), 8 orders, 11 families, 26 genera and 24 species were identified. The most abundance and biomass of orders in the different seasons were belonged to polycheata (annual average: 2452±258 g.m-2) and bivalvia (annual average: 25±6 g.m-2), respectively. Macrobenthic abundance in all orders except insecta and oligocheata in different seasons was significant difference. The amount of seabed total organic mater (TOM) from the 5m to 100m stations was with an increasing trend and the highest percentage had in the 50m and 100m stations (P〈0.05). The percentage of seabed TOM was not significant difference in the different transect (P〈0.05). However, seabed grain size had significant difference (P〈0.05) and seabed grain size from the 5m to 100m stations was with a decrease trend in the size (P〈0.05). Macrobenthos and physicochemical parameters of correlation matrix showed that macrobenthos abundance in the between parameters have a positive correlation with TOM. However, it biomass with pH (positive), inorganic phosphorus (positive) and organic phosphorus (negative) have a correlation. Heavy metals: The levels of heavy metals (Cd, Cr, Cu, Fe, Pb, and Zn) of water had lower than standard and acceptable for aquatic life. Significant accumulation of metals such as Co, Cu and Cr in the sediment was observed at transect Sefidrud and Anzali and Ni at transects Sefidrud and Astara in comparison with six other transects (P〈0.05). Petroleum compounds (16PAHs): Concentration levels changes of petroleum compounds in seawater in different seasons was significant (P〈0.05) and in seasons of spring, summer, fall and winter was 56.5, 9.3, 14.9 and 19.3 of percent respectively. However, the concentration values in sediments of the sea in different seasons and transects were not significant difference. Maximum concentrations in summer and in Astara transects (8.02 mg.kg-1) were determined. In correlation matrix, observed positive correlation between petroleum compounds and Cr, Ni and Pb in sediments of seabed and between petroleum compounds and Zn in seawater. Organochlorine pesticides (OCPs): The OCPs concentration of seawater in different seasons had significant difference (p〈0.05). The pesticides groups of DDT, BHC, Drin and Endosulfan in seawater in different seasons had significant difference (p〈0.05), but, it was not found in Heptachlor group (p〉0.05). The maximum concentration values of OCPs in all groups in seawater were determined in winter (99%) (p〈0.05). The seawater OCPs concentration in comparison of regional and stations were not had significant differences (p〉0.05). The only DDT group in the seabed sediment had significant difference in different seasons (P〈0.05) and the maximum of seasonal average, it was determined in summer (Average: 0.88±062 mg.Kg-1 and Max: 4.5 mg.Kg1). However, in comparison of regional were not had significant differences (p〉0.05). In correlation matrix, the groups of Endosolfan and Drins in seawater and sediment were had equal variance and the changes were included. There was high correlation of between Endosulfan and Drin with DDT group in seawater and with Heptachlor group in the sediment of seabed. Surfactant: Mean surfactant concentration (LAS) between different seasons had significant difference (P〈0.05). The maximum concentrations of LAS were in spring and summer (Anzali transect) was 0.084 mg.l-1 and 0.082 mg.l-1, respectively. Also, average concentrations in the spring: 0.06±0.003 mg.l-1, summer: 0.059±0.004 mg.l-1, winter: 0.042±0.003 mg.l-1 and fall: 0.031 ± 0.013 mg.l-1 was determined. Nevertheless, there was no significant difference in the study area and station (p〉0.05). Based on existing standards, Surfactant concentration in the southern Caspian Sea region was not critical.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Over-fishing of marine resources has endangered many commercial fish species in the world; with aquaculture development, setting up artificial reef systems is an important way for marine stocks and fishing enhancement. The present study was designed to monitor fish abundance and species changes around a small and newly established artificial reef system in Moloo area at Bandar-e-Lengeh during two years after installation (Sep. 2005 to Sep. 2007). The artificial reef includes three types of concrete structures arranged in a seven by three grid. Each cross point considered as a sampling station and two other stations selected from two sides of the system as control stations. Based on obtained results CPUE (P = 0.00001), frequency (P = 0.001) and species diversity of captured fishes (P = 0.024) showed significant differences between three types of sampling traps. With type of structures, The CPUE and frequency of fishes in transect 7(mixed structures) showed the significant differences with other six transects (p= 0.001, P = 0.009). No diversity relationships were seen between transects (p= 0.100). In this study there were no significant differences between depths. Although the means of CPUE between seasons were different, but the ANOVA test could not show the significant differences because of the differention between variances. The T-Test showed no significant differences between the number per trap per day dominant species (Epinephelus coioides, Plectorhinchus shotaf, Diagrama pictum, Siganus javus ) in different seasons. Movie prepared from artificial reefs showed diversity of fish were more than that of fishing by trap. Although fishes increased but there was no enough causes evidences for product in artificial reefs. Therefore, the study need to continue in this area.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Location of family Satellite DNA (HindIII SatDNA) on chromosomes of Acipenser persicus and Acipenser gueldenstaedtii was analyzed using Fluorescent In Situ Hybridization (FISH) technique to determine the genetic differences between these two species. After obtaining suitable metaphase plates using leukocyte culture and HindIII SatDNA extraction from genome of the fish under study, extracted SatDNA from genome of A. gueldenstaedtii was used as a FISH probe. The probe was labeled with spectrum orange (Orange-dUTP) and hybridized with chromosomes of the fish under study. Analysis of SatDNA sequences isolated from A. persicus and A. gueldenstaedtii showed the presence of 163bp for A. persicus and 168bp for A. gueldenstaedtii. In the study of metaphase plates 66±4 signals were detected from the hybridization of the probe used with chromosomes of A. persicus and 68±3 signals were detected from the hybridization of the probe with chromosomes of A. gueldenstaedtii. Due to presence of numerous microchromosomes and heterogenous and large hybridization signals, it was impossible to identify the precise position of signals on chromosomes. The following results are worth mentioning: 1. Developing leukocyte culture method for A. gueldenstaedtii and completing it for all of the Caspian Sea sturgeons. 2. Developing a suitable culture medium for the Caspian Sea sturgeons. 3. Isolating sturgeons HindIII Satellite DNA family from A. persicus and A. gueldenstaedtii, determining sequences and registering the sequences in the NCBI gene bank (FJ429174; FJ 94465). 4. Developing a protocol for FISH technique in A. persicus and A. gueldenstaedtii which has opened a new era in aquaculture genetic studies entitled Molecular cytogenetics in the country. Considering its potential applications, this technique can be applied to other aquatic species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study focused on population structure, biodiversity, and abundance of the Chitgar Lake fishes in October 2014. Based on the lake location fishes samples were collected at 34 stations in water body. In this study identified 8 family belong to 18 species comprised of Cyprinidae (11 species), Cichlidae (one species), Loricariidae (one species), Pangasiidae (one species), Serrasalmidae (one species), Poeciliidea (one species), Salmonidae (one species), and Scaridae (one species) were Identified. Among the fishes just one native fish from Capoeta bohsei species observed during the study. The dominant lake fishes were invasive species Hemiculter leucisculus, Alburnus hohenackeri, Carassius auratus, Carassius gibelio and Pseudorasbora parva. The highest abundance of fishes were H. leucisculus 62 % and A. hohenackeri 35 % by Seine net. Furthermore, those were dominant abundance (69 and 12 % respectively) in Gill net method. Finally in Cast net method A. hohenackeri 38 %, Pseudorasbora parva 21 % and H. leucisculus 17 % were dominated in the shore region. The lowest fishes abundance were P. hypophthalmus and Parrotfish. Overall more than 90 % of the fishes population were invasive and alien species. Thus, the present of invasive fishes and predator fishes luck in the Chitgar lake might be reason for ecosystem ruin, algae bloom, feed competitive, fish disease and trophy level rises.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The present study was conducted from autumn 2003 to summer 2005 with the objective to estimate distribution and relative abundance in different species of sturgeons as a function of seasons, and regions in the west coast of the Caspian Sea (Guilan province).Gillnets with a different mesh sizes (26, 33, 40, 60, 100, and 150 mm) were set up at different depths(2, 5, and 10 m) for 24 h. Data on variations in catch, catch per unit effort(CPUE) and length and age composition of sturgeon species were analyzed statistically. Data on CPUE in each region and depth and mean estimates of length and age in fish were reported for each season and each year of the study period. CPUE during 2004-2005 was 1.32 fish which was 30.1% lower than CPUE (1.89 fish) recorded in 2003-2004.CPUE for all species except A. nudiventris decreased during 2004-2005 as compared to that in 2003-2004. Acipenser persicus caught during 2003-2004 and 2004-2005 ranged from 15 to 45 cm in length and comprised 91.1 and 97.1% respectively of the total sturgeon catch. These fish belonged to the one year age class. Mean length recorded in sturgeon fish caught in 2004-2005 decreased as compared to that recorded in 2003-2004. Significant differences were recorded in total length of fish caught in 2003-2004 and 2004-2005. Results obtained from the present study reveal that the abundance of fish increased from west to east indicating a direct relationship between the general currents found in the Caspian Sea and the higher density of nutrients in the eastern region.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Stock assessment of sardine and anchovy fishes in Persian Gulf and Oman Sea (Hormuzgan province) using fish population dynamics parameters and stock assessments models were carried out to estimate maximum sustainable yield (MSY) from 2010-2012. Results of 16 years (1996-2012) fisheries data analysis showed the mean of CPUE (catch/shooting) were calculated for Gill nets, Beach siene and Purse siene as 134, 988 and 2338 kg/shooting respectively. Relationships of catch and effort were determined for Qeshm area (R2=0.94) , Bandar Lengeh area (R2=0.51) and Jask area (R2=0.73) as a polynominal increasing model. Population dynamics parameters using monthly length frequency of 10540 sardine and 8232 anchovy by FiSAT II from Persian Gulf (Qeshm-Bandar Lengeh) and Oman Sea (Jask) fishing areas. Growth parameters K and L∞ of sadine (Sardinell sindensis) were estimated 1.18 y-1 and 19.1 cm in Persian Gulf and 1.11 y-1 and 19.1 cm in Oman Sea, and also for anchovy (Encrasicholina punctifer) were estimated 1.20 y-1 and 9.2 cm in Persian Gulf and 1.18 y-1 and 9.3 cm in Oman Sea. Anually, 5 cohorts for sardine and 2 cohorts for anchovy were estimated using Bhattacharya method in Persian Gulf and Oman Sea. Natural mortality (M) and fishing mortality (F) were estimated for this species, and exploitation rate of sardine were calculated as 0.58 and 0.44, and exploitation rate of anchovy were 0.54 and 0.34 in Persian Gulf and Oman Sea respectively. Length of catch (LC) were calculated 11 cm and 12 cm, and for anchovy 6.8 cm and 6.6 cm in Persian Gulf and Oman Sea respectively. Length – weight relationships of sardine and anchovy were calculated as isometric growth. Results of this study showed that populations of sardine and anchovy in Qeshm and Bandar Lengeh area are a single-unite stock, no enough evidence for discrimination of sardine and anchovy populations in Persian Gulf and Oman Sea. The mean anuall biomass of sardine and anchovy were estimated using Thompson and Bell model 190000 tones in Hormuzgan province. Maximum sustainable (MSY) of sardine and anchovy were estimated between 59721 and 98309 tones in this area. MSY of sardine were calculated 15222 and 3182 ton and MSY of anchovy were 36237 ton and 5080 ton , for minimum of estimated MSY in Persian Gulf ( Bandar Lenghe-Qeshm) and Oman Sea (Jask) respectively. Using yield per recruit of Beverton and Holt model , F0.1 were estimated averagely 1.75 for sardine and anchovy in Hormuzgan province and its showed the underfishing condition. Survey of catch and efforts trend in sardine and anchovy, defined development phase of small pelagic fisheries in Hormuzgan province.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Low hatching artemia cysts which has no use in the aquaculture can be decapsulated and directly use in larvae culture. In order to increase the knowledge of consumers to know the quality changes of low hatching decapsulated cysts during storage after production (especially fatty acids ) this research was carried out. In this work Low hatching cysts of artemia urmiana had decapsulated with sodium hypochlorite and other chemicals. We used drying room and f.b.d system for drying of this decapsulated cysts and package units had used for packing this product to cans. We had three experimental treatments of dry decapsulated cysts (3 type packing including vaccumed cans and free (plastic dishes). These treatment were evaluated at different times (comparing of before produce, after produce , 3, 6 and 12 months keeping after production ) such as fatty acids , fatty percent , protein percent , bacterial and fungal contamination and presence of aflatoxin the result of fatty acids using one way anova analysis showed that the variation of half of fatty acids specially DHA and EPA were significant ( p 〈 0.05 ) . Fatty percentage increased and protein was decreased about 12-15% during the one year storage we separated two species of bacteria and two species of fungi but they had no pathogenic bacterial and fungal. During 6 months storage the quality of products have less variation compared whit 12 months.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Aquaculture for human consuming species is being considered as the first substitution of catching aquatic species due to increase of human population and decrease of wild aquatic stocks. In this study, the hybrid sturgeon Bester (female beluga x male sterlet ) was produced for the first time in Iran. Sperm of 1.35 kg male Acipenser ruthenus was used to fertilize the eggs of 125 kg female Huso huso in Shahid Marjani Sturgeon propagation center (Agh Ghala, Golestan province). The fries of bester and control treatment of beluga were transported to International Sturgeon Research Institute (Rasht) after about one month by reaching to 490 mg and 377 mg of weight respectively. All fishes fed by artificial concentrated food (48-50% protein and 15-17% fat) after a period of feeding with Artemia and Daphnia. Sorting was carried out according to increase of fish weight for both fishes. Results showed that the imported sterlet spawners were not at the high maturation stages and especially the males had not suitable sperm quality. It showed that up to 2 months of age , these was no significant difference between bester and beluga weight but from this age up to 2 months of age the weight of beluga was greater. Meanwhile from 2 months old up to the end of the study (21 months) the weight of bester sample was significantly greater than beluga. The comparison of FCR for the whole rearing period showed no difference between bester and beluga (2.4 and 2.3 respectively). In general, the increase and decrease pattern of GR and SGR were coincided to each other, but showed monthly differences. Growth rate (GR) and specific growth rate (SGR) of bester were greater than beluga from 4th and 3rd month of rearing period respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Anzali wetland at south west of Caspian located along of North West and southeast and with its unique feature were add on the Ramsar convention list in 1975. There were recorded a high rich of fauna and flora including 276 plant species, 23 reptiles and amphibians, 60 fishes, 26 mammals and 195 birds. Anzali wetland were encountered with numerous disorder with the eutrophication and the early dismissing. The first studies on Anzali wetland started by many international institutions in 1964. In this study the data of biotic and abiotic factors in Anzali wetland analyzed which belongs to different sites (15 to 38 stations) during 1991 to 2000 and were sampled seasonally or monthly in different years. The results showed the increasing trend of phytoplankton abundance from 7.9 to 66 million C./l. and the western part had the most abundant. The Cyanophyta had the most development than other 7 phylum observed in the area. In Anzali wetland identified 143 phytoplankton genus that 9 genus dominated in more than 80 % replicates and 7 genus had the abundance of more than 5 million cell/l. The Zooplankton abundant increased from 580 to 2200 ind./l. The Rotatoria had the most abundant followed by Copepoda. There was identified 104 zooplankton genus that 5 genus were dominated in more than 80 % replicates and 11 genus had the abundance of more than 30 ind./L. The chlorophyll a varied from 8.8 to 50.2 μg/l. and had a increasing trend after 1996, meanwhile it was measured higher than 25 μg/l in most of the stations. The results of benthic study showed that the Chironomidae and Tubificidae were observed more than other organisms which comprised 12.2 to 23.5% of total biomass of benthic organisms in studied period. The range of biomass was 1.1 to 7.8 g/m2. The ichthyologic survey showed presence of 60 species that 11 species were exotic. 28 species belong to freshwater, 17 species were anadromous and 8 species were resident in estuary. The highest and lowest fish catch were 640 and 288 ton in 1994 and 1999 respectively and it was about 500 ton at the end of period. The hydrochemical factors had the various fluctuation so that the nutrients parameters had a significant difference over studied period. Total nitrogen was varied from 0.78 to 1.41 mg/l and total phosphorus varied from 0.05 to 0.1 μg/l.. The oxygen dissolved amount was from 4.6 to 8.7. EC was between 3 to 5 ms/cm during September and October that indicated of Caspian water projection. According to geographic position of station, all biotic and abiotic data, were arranged. The data bank is used by Idrisi software to produce of necessary maps. Analysis of data showed that there must be a responsible department with a vast authority. Conservation and eutrophication prevention of Anzali wetland connect to special attention to catchment area and landuse management of the wetland. Also there is an urgent need for well-developed technologies and managements to reduce the organic pollution and its environmental impacts on the Anzali Lagoon.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Without abstract.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Artemia has important role as live food in aquaculture nutrition. There are bisexual and parthenogenetic strains of Artemia urmiana which are living around the Urmia Lake. Selection of suitable strain for culturing programe must be considered at aquaculture industry. In order to find suitable at both strains for, the evaluation of ovoviviparous and viviparously characteristics must be done. The objective of the present study was to introduce the suitable generation for both characteristics (cyst & nauplii production) in bisexual and parthenogenetic strains of Artemia urmiana at urmia lake, Iran. This research was carried out at laboratory under similar and fixed experimental condition for culture. Each artemia was fed with live Dunaliella tertiolecta species .Adults at each generation were assessed the number for frequency of cyst and nauplii production and mortality rate. Results with using one-way ANOVA &Duncan test by SPSS software were analyzed. The results showed that there is significant difference among 10 generation at bisexual strain of Artemia urmiana for cyst-production and mortality rate (P〈0.05) and this variation was not significant for nauplii production.In addition that the seventh generation had high cyst-production and sixth generation had high nauplii-production in bisexual strain. The results showed that there is significant difference among 10 generation for at parthenogenetic strain of Artemia urmiana around urmia lake for cyst and nauplii production and mortality rate (P〈0.05). In addition that the seventh generation had high cyst-production and tenth generation had high nauplii-production in parthenogenetic strain. Totally, adults of bisexual strain was suitable for cyst-production and parthenogenetic strain for nauplii-production was suitable. The mortality rate had high at bisexual strain compared to parthenogenetic strain .It can be concluded that the seventh generation for cyst-production were suitable in both strains and sixth generation for nauplii-production in bisexual strain and tenth generation for nauplii-production in parthenogenetic strain were suitable. It is recommended to select seventh generation for cyst-production at both strains and tenth generation for nauplii-production at parthenogenetic strain studied.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to have a sustainable management on Persian sturgeon (Acipenser persicus) as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Black Mouth Croaker (Atrobucca nibe) is of significant value from the economics point of view. In the following study, the genetic diversity of Atrobucca nibe has been investigated using the PCR-sequencing method from three different regions of the Persian gulf and Oman Sea, namely Chabahar in the Sistan and Baluchestan and Jask in Hormozgan Province of Iran and Fujairah in the Fujairah Emirate of the United Arab of Emirates. The purpose of the following exploration has essentially beencoined to further the study of Atrobucca nibe genetic diversity and its population structure in the Persian Gulf and Oman Sea. For this purpose, a number of 54 fish have been collected from the aforementioned regions. The DNA of the fish had been extracted through the Ammonium Acetate method and the quality of the DNAs were analyzed via the spectrophotometry and furthermore the quantity of the extracted DNA were assessed viaelectrophoresis. Polymerase Chain Reaction (PCR) was conducted on the targeted DNAs and thenceforth DNA sequencing was carried out. Subsequently, through sequencing it was discovered that the D-loop region in the mitochondrial DNA (mtDNA) of Black Mouth Croaker,contained 270 base pairs (bp). For the purpose of analyzing the genetic diversity of Atrobucca nibe and its population structure in the Persian Gulf and Oman Sea, the following softwares have been conducted, namely theBioEdit, Arlequin, Dnasp and Mega5 software. Furthermore, the Kimura 2-parameter was used for the purpose of genetic distance analysis. Through observations of average levels of FST between the regions, it can be summed up that genetic differences do exist among the present populations. On the grounds of the obtained results from the Persian Gulf and Oman Sea, it can be concluded that there currently are three different populations of Atrobucca nibe residing in the waters of Sistan & Baluchestan, Hormozgan and Fujairah.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was aimed to investigate the effect of different doses of oral diethylstilbestrol (DES) on feminization of Nile tilapia (Oreochromis niloticus) larvae under the condition of brackish water in Bafgh fisheries research center. This Experiment was comprised of two control groups in two replicates and two experimental ones in three replicates. At the beginning of the trial, a number of 2150 larvae were randomly distributed among 10 replicates that were under treatment for 45 days. The diets in two control groups, i.e. non-treated, C1, and ethanol treated feed, C2, and the ones in two experimental groups, i.e. 200 and 400 mg DES/k of feed, E1 and E2, were administrated to 7 day post fertilization (dpf) larvae for a period of 35 days and then until day 45, the larvae were fed with ordinary feed. At the end of the trial, the gender ratio of larvae in different groups was determined by squash test in 50 randomly selected individuals from each replicate. The results showed no significant differences for gender ratios within control groups (respective male to female ratio in C1 and C2 was 47:53 and 57:43). However, the corresponding ratios within experimental ones, fed with 200 and 400 mg DES/k of feed respectively, was significantly different (P〈0.05). Noteworthy that the percent of females between two experimental groups (E1 and E2) was not significantly different (70.0 and 64.7 respectively) but it was significantly different with ratios in experimental groups (P〈0.05). The total body length and live body weight were determined at the begging and then at day 30 and 45, it was randomly measured in 20 larvae from each replicate. At the beginning of the experiment, the mean body weight and total length of larvae was 0.012 gram and 1.03 centimeter respectively. The respective averages at day 30 and 45 were significantly different among groups (P〈0.05). Survival rate was significantly different among groups (P〈0.05), being highest in E1 and E2 respectively whereas lowest in control ones (56.51 and 58.84 in C1 and C2 respectively).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Annually, increasing antropogenic impact on environment results in a considerable aggravation of the ecological condition and extinction of many species of organisms and a decrease in the abundance of Acipenseridae fish family in the Caspian Sea. Therefore cryopreservation of the sturgeons sperm has become on urgent problem. Thus cryopreservation of gametes and embryos is considered to be one of the ways to preserve gene pool of the fish. In this research the broodfish originated from Turkman and Tazabad fish capture station in south of Caspian Sea. They were stored in fish breeding pond with river water flow for one week in shahyd Marjani and shahyd Rajaei fish farms. The males of broodfish were injected intramuscularly with action dryed sturgeon pituitary at doses of 2.5 mgkg-1 body weight. The sperm was collected 12h later, by a sound connected to 50ml syringe. Activity of fresh sperm wa determined by mean of microscope after its dilution by water. Then the fresh sperm was diluted by cryoprotective medium in proportion of 1:10 containing 0.1 M of Tris-HCl buffer, pH 8.6, 2% dimethyl sulphoxide, 25% yolk of chiken egg and 0.04 M saccharrose. sperm diluted using this way was poured into 1ml syringe and frozen according to two method: immediately or gradually. After 12h to 205h activity of frozen-thawed sperm was determined and used for fertilization. As the result showed that the percentage of foucoundation (success of fertilization) was 27 and the percentage of yolk sac embryo stage was 16.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to determine water quality and sediment of Aras River based on environmental parameters, metals (Mo, Cu, As, Hg) and determine of heavy metals in fish species at four stations (From Julfa to 25km after Nourdos border) during four seasons from 2015 to 2016. All samples were carried out standard methods using titration and spectrophotometer instruments. Result showed that range of water and weather temperature, DO, BOD5, COD, EC, Turbidity, TA, CO2, pH, DIP, NH4/N, NO2/N, NO3/N and IRWQI were observed 200-24.00, 3.00-28.00 C, 8.90-15.01, 1.77-13.41, 2.00-86.00 mg/l, 1.00-1.34 ms/cm, 2.4-638.0 NTU, 116-247 mg/ CaCO3 l, 0.20-2.20 mg/l, 8.06-8.79, 0.026-0.195, 0.330-0.575, 0.005-0.026, 3.640-6.474 mg/l, and 31.10-48.02, respectively. Mean of whole parameters (TA, NO2 - and NO3 - ) were significant between seasons and (p〈0.05) but whole parameters were not significant between stations (p〉0.05). Result showed that range of Mo, Cu, As, Hg in water and sediment were observed 0.10-23.01, 3.0-40.0, 0.02-0.81, 23.7-104.6 µg/l, 0.1-111.6, 17.5-482.5, 0.001-0.145, 46.8-303.6 µg/g.dw, respectively.As a conclusion, water quality of Aras River was classified in class III (moderate pollution) based on mean concentration of Cu and As concentration was observed less than threshold level. Water quality of Aras River was classified in class IV and II based on mean value of Hg and As, respectively. Maximum value of Mo and Cu at surface sediment was registered at stations 3 and 4 during all seasons which it is because of effluent of sewage from Mo, gold and other metals mines in Armenia country. Mean concentration of As at surface sediment was higher than standard value, but concentration of Hg was lower than threshold value. Bioaccumulation factor was also calculated for each species.The amount of Bioaccumulation factor for molybdenum is the highest and the arsenic metal has the lowest amount. The highest concentration of copper in Barbus lacerta with a mean of 13.6 ± 11.3 μg / g dry weight and for the molybdenum, Alburnoides bipunctatus had the highest mean concentration (11.7 ± 4.9) μg / g dry weight. The average concentration of mercury (13.1 ± 1.5 μg / kg dry weight) was highest in Alburnus alburnus. Regarding the arsenic element, as it was analyzed in a small number of specimens, the highest concentrations were recorded in Alburnoides bipunctatus . According to ANOVA, there is a significant difference between mean concentration of copper in different species (p 〈0.05) and the Duncan test has distinguished Alburnus alburnus from other species. As a conclusion, result showed that 61 percent of the total data indicates the quality of water was relatively bad, although about 31 percent of the total data (based on the quality of IRWQI) indicates the quality of water was "moderate level.This condition starting serious changes in water characteristics affected by environmental degradation, exposure to domestic and agricultural pollution.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Harmful algal blooms resulting in red discoloration of coastal waters in the Persian Gulf, Iran were first observed in January 2007. The species responsible for the bloom, which was identified as Cochlodinium polykrikoides, coincided with massive aquatic organisms’ mortalities in the Persian Gulf. In order to provide optimum growth and bloom forming, C. polykrikoides cells were sampled during the bloom conditions in the coastal waters of Persian Gulf. After adaptation in filtered seawater, they isolated by positive phototropism characteristic of this species to light. They were grown in modified media culture at different salinity (30, 32 and 35ppt), temperature (20, 23, 26 and 28ºC) and intensity (35, 70 and 90 µmol m-2 s-1) with an initial cell density of 50 cell mL−1. The results of the present study clearly showed that the highest alga biomass was obtained following culture by using A2 medium under the 32ppt salinity, 26°C temperature, and under a 11h light:13h dark photoperiod regime at a light intensity of 90 µmol m-2 s-1 provided by cool white fluorescent tubes. Mean cell density of C. polykrikoides in a 60 liter tank for ten days reached to 32 × 106 cell L−1. Moreover, individual C. polykrikoides chain with 18 cells was observed for the first time in cultures. Based on the results from the present study, as mentioned above, providing suitable media culture and physical condition (light intensity and temperature), bloom forming of C. polykrikoides start from day 8 to 10 and will be continued until day 24 t0 28. In the other hand, C. polykrikoide cells immediately crashed and destroyed.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Annually, many data of hydrology and hydrobiology of the southern Caspian Sea at Iran's coasts were collected by Caspian Sea Research Institute of Ecology. The data which had been collected by researchers submitted annually in several reports but has not been processed all at once. Fluctuations of various parameters were surveyed and the relationships between them were analyzed. The main goal of this research is to analysis the data were obtained from 1994 to 2006 and show changes the concentration of various parameters in the Caspian Sea. Physicochemical data indicated that the average trend of transparency and salinity was decreased from 1994 to 2006 annually, where the salinity value was decreased from 12.37 ppt (part per thousand) to 11.5 ppt. The average of dissolved oxygen was increased from 6.4 to 8.6mg/l. pH has slightly fluctuated from 8.15 to 8.31. However, with increasing depth from surface to bottom, the average of dissolved oxygen was sharply decreased (DO concentration in surface and 800 m was 7.18 and 1.45 mg/l, respectively). The trend of salinity and pH fluctuation was very slow with depth but the salinity has an increasing with incrementing of depth but pH has a decreased trend. The average of organic and inorganic phosphorus and TP was nearly increased. The average of Nitrate and Silica sharply increased comparing to depth but the average of Nitrite and organic Nitrogen decreased. The results showed that the temperature fluctuation in different water layers was low in winter but the dramatic decreasing of temperature was occurred at 10-20 m (in spring), 20-50 m (in winter) and 50-100 m (in autumn). With increasing of depth (Slope of the Sea), transparency and salinity values have an increasing while DO and pH showed decreased. As a whole, 335 species of phytoplankton were identified, there are 70 species in advance as these species were not seen in this period but 96 of novel's species have been recorded. The lowest and the most of phytoplankton biomass was recorded in 2006 and 2001 while the biomass was 59 and 1034 mg/m3, respectively. Albet, the lowest biomass was recorded at the depth of 100 m (24.1 mg/m3) but the maximum was observed in surface layer (1344.1 mg/m3). The average of phytoplankton biomass was increased after arrival of the ctenophore. There was a significant difference between the average of biomass in different seasons before and after of the ctenophore invasion (P〈0.001). Bacillariophyta and Pyrrophyta have the most cell abundance with a total of biomass of 52.7 and 37, respectively. From 1994 to 2006, species diversity (Shanon-Niner), evenness and richness were estimated between 3.02-1.29, 0.28-0.61 and 4.32-7.60, respectively. A total of 65 zooplankton species were identified with a frequent species in the Caspian Sea before the ctenophore invasion, while the species diversity decreased after the invasion. The high and the lowest of species diversity was recorded in 1994-1995 and 2006 and in 1999 the biomass of the zooplankton were observed between 12.6 mg/l and 363.8 mg/l, respectively. After arrival of ctenophore, the averages of zooplankton biomass at of the different depths were sharply decreased and were less than the ctenophore invasion. The result showed that there was a significant difference among the average of zooplankton biomass in two periods, seasons and west, middle and east regions as follows: P〈0.001, P〈0.008 and P〈0.01. The maximum abundance and biomass of zooplankton belonged to copepoda with %51.5 and %63, respectively. Rotatoria falls in the second class from 1994 to 2006 (during 1994-2006), species diversity, evenness and richness were varied between 0.19-1.6, 0.08-0.52 and 0.37-2.29, respectively. The species diversity of macrobenthic organisms at the same period s followd by zooplankton changes which sharply decreased. The average of benthic biomass reduced from 13.7 g/m2 in 1994-1995 to 1.8 g/m2 in 2004 but sharply increased in 2005 with a maximum value of 46.7 g/m2. With increasing of depth, the average of benthic biomass was sharply decreased. There was a significant difference (P〈0.001) in macrobethic organisms abundance before and after the ctenophore invasion, but it did not show a significant difference between seasons and different regions (as follows: P〉0.137, P〉0.782). Before the presence of ctenophore, the worms had been constituted a dominate group with a approximately %45 of total abundance and also %20 of their biomass. After this period, their frequency was sharply increased (more than %85) but the maximum value pertains to Cardidae (more than %90). The species diversity, evenness and richness were varied between .69-2.51, 0.23-0.63 and .011-3.79, respectively. AMBI software, Shanon-Viner parameter was moderate in all seasons from 1994 to 1996 but this parameter was reduced in a bad limit (boundary) in the most seasons from 2002 to 2005. The main parameter of M-AMBI consists of several parameters and also approximately showed similar changes such as Shanon -viner parameter. On basis of this parameter, the ecological quality condition of Caspian Sea was superior limit in all seasons from 1994 to 1996 but after the following years were decreased from good to moderate limits (After the presence of ctenophore). Relationship between abiotic variants and phytoplankton indicated that there was a direct relationship between transparency and salinity while there was a powerful and reversal significant relationship between transparency water temperature, transparency in organic Nitrate, phytoplankton number and transparency phytoplankton biomass. The comparison between different variants average in two periods (before and after the ctenophore invasion) indicated that the average of Kilka catch was decreased, the relative frequency of clupeonella engrauliformis and clupenoella grimmi was sharply reduced but the relative frequency of Clupeonella cultriventris was sharply increased, the species diversity, evenness and richness and the number of zooplankton species were sharply decreased, the average of biomass and transparency was sharply reduced, the average of dissolved oxygen and liza saliens or liza auratus catch were increased. These difference were often significant (P〈0.05). Therefore, with the ctenophore invasion into the Caspian Sea, the primary production was increased, the biomass value of zooplankton and specially Kilka which fed on zooplankton were sharply decreased while the fish such as Rutilus frisi kutum and mullet (Liza salins or Liza aurratus) which fed on benthic were increased.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreementmade between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. The study has several phases. The final report of the fishery on fishing and resource management of fisheries within Southern Caspian Sea (Gilan, Mazandaran & Golestan provinces) is consisted of several sections such as: 1- The Survey status of biology and catch of Kilka fishes , Bony fishes and Estrogen fishes and also the status of aquatic resource conservation in the Southern part of Caspian Sea 2- Fishing analysis in the Southern part of Caspian Sea 3- Fisheries development Strategies, policies and plans in the Southern part of Caspian Sea In this survey and library-based study, internal and external environmental factors of shilat activities are considered. Using SWOT analysis method, current situation of fisheries is investigated. In this regard, all fisheries activities were divided into four groups, then, the strengths, weaknesses, opportunities and treats of internal and external environment will be analyzed. A considerable number of tables and matrixes were prepared and policies and strategies as seen of final report were carried out.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In recent years MPAs (Marine Protected Areas) have been known as a flexible tool to improve fishery management as well as to preserve biodiversity in valuable coastal waters habitats. This study was conducted to selecting the sensitive and high potential area that are impacted by several environmental stress and associated habitats are being damaged in Mahshahr creeks in Northwest of Persian Gulf. Study was done in eight creeks from October 2005 to September 2006. Different biotic parameters such as phytoplanktons, zooplankton, macro zooplankton, benthic animals and fishes (trawled species) communities and also physico-chemical parameters and sediments heavy metals were investigated. Different parameters were sampled monthly or seasonal from each creek. Water samples with bottle sampler and sediment were collected by Peterson grab. Several ecological and biological indices based on heavy metals and benthic communities were used. Concentrations of heavy metals were measured by using voltammetric plarography method (Methrom 797). The range and mean concentrations obtained in mg/kg were 35.16-15.03 (27.01) for Cu, 171.41-65.57 (102.672) for Ni, 20.06-4.63 (13.22) for Co, 0.78-0.093 (0.22) for Hg, 379-65.07 (113.7) for Zn, 1.00-0.27 (0.559) for Cd and 29.72-7.09 (14.66) for Pb. To evaluate the levels of sediment contaminations, the background values of the different heavy metals were calculated and contamination factor for each metals and degree of contamination for each creek determined as well. Measured concentrations were compared with International standards. Ultimately the heavy metals contamination factors (Cf) can be arranged as follows: Hg〉 Zn〉 Cu≥ Ni〉 Pb≥ Co 〉 Cd and according to contamination degree (Cd), different creeks can be arranged as follows too: Ghannam〉 Ahmady≥ Zangy〉 Doragh≥ Darvish〉 ghazaleh〉 Patil〉 Bihad Regarding the results some elements such as Hg, Zn and Ni, are at risk level and all of the studied creeks are classified in moderate degree of pollution except Ghannam showing considerable degree of pollution. The benthic invertebrates are a well-established target in evaluations of environmental quality status. The AMBI(AZTI,s Marine Biotic Index) was developed to determine the impacts and the quality status in softbottom marine benthic communities. All creeks are characterized by muddy bottom. Macrobenthic animals, according to their sensitivity to an increasing stress gradient, classified in five ecological groups. In present study due to appearance of dominant species such as Capitella sp and nematodes (as opportunist species), diversity values was reduced. Two way ANOVAs showed only seasonal significant differences in mean abundance (P〈0.05, f=5.712, df:3,21) and Richness index values (p〈0.05, f= 4.975, df=3,21), while all of creeks showed similar biological characters based on benthic communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, The most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution (32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. Communities. According to annual, mean of AMBI (BC) all of creeks classified in ecological group III with slightly pollution, except Darvish that was placed in unpolluted category. In general, according to AMBI and BI values, The most creeks are classified in unpolluted (34%) and slightly pollution (34%) categories except Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter that showed moderate to heavily pollution(32%). The results of chemical quality of sediments, Cf values, Cd values, AMBI Index and water quality and risk Indices have confirmed each other. In general, the Mahshahr creeks are classified in moderate to heavy pollution status. In addition, biological parameters of benthic communities and other biotic parameters have showndescending trend in ecological quality in all of the studied creeks. We used of some criteria for evaluation of biotic potential or sensitivity and also to explain the ecological health level of studied creeks. Positive and negative criteria are classified in three level 1,3 and 5 for low, moderate and high degree of conservation value respectively. According to gained points the creeks Darvish, Doragh and Ghazaleh in high level and creeks Ghannam , Zangy and ahmady in low level of conservation value were classified.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Mullet and other bony fishes are caught by beach seine in Iranian waters in Golestan, Mazandaran and Guilan provinces. In this investigation which had conducted in commercial regions during 2003-2004, we estimated catch and CPUE, species composition, length, weight, age of two species of mullet and Salmo trutta caspius; von Bertalanffy growth parameters, mortality, biomass and MSY of Liza aurata. The total recorded catch of mullet was 3870 mt and L. auratapre dominated (98.5% of total catch of mullet). CPUE of two species of mullet; L. aurata and L. salienswere 69.6 and 1.1 mt purse seine, respectively. The catch of Salmo trutta caspius was negligible (about 3.6 mt). Biomass and MSY (maximum sustainable yield) of L. aurata were estimated to be 11019.8 and 3322.6 mt, respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This report is about coral-reefs around Abumosa lsland from January 1998 till February 1999 by trap and underwater visual census. Total species belonging to genus and family were idemtified and length-weight relationship were estimated for more frequent species. Small tooth emperor (Lethrinus microdon) was predominate from other species. Catch per unit of effort (cpue) calculated for each trap (large, medium and small). Efforts were based on number of trap and day. Cupue of small and large traps was further in late summer compare other seasons. Results of catch analysis showed that no differ catch value (eatable, fishes, ornamental fishes and total catch) between small-medium and medium-large traps. Value catch wasn t significant difference between three depth (0-10, 10-20 and 20-30m) and four geographical side, too. But, catch value of ornamental fishes was significant difference between depths 0-10 and 20-30 meter.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Primary researches on aquaculture of endemic shrimp species carried out since 1984-5 by Iranian Fisheries Research Organization (IFRO) .After a decade, the condition for extension and development of shrimp culture in the country prepared. Despite huge investment of government for preparation and construction of shrimp sites, only small part of present potential in southern coast of the country is under production. About 18 different shrimp species inhabit in Persian Gulf and Sea of Oman, in which 4 species have larger size, and more abundant, and harvest commercially . Penaeus semisulcatus, Fenneropenaeus merguiensis and Metapenaeus affinis as commercial species distributed throughout of Persian Gulf from mouth of Arvand River in north- west to strait of Hormoz, and also some parts of Oman Sea. While Fenneropenaeus indicus distributed mainly in coastal area of Oman Sea. Shrimp culture begin in Iran by P.semisulcatus, continued by imported Penaeus monodon, and developed by using of F.indicus. After confront to slow growth and viral diseases in 2001 (Khouzestan province) and 2005 (Bushehr Province), at the same time I.F.R.O acquired the bio-technique for production of Litopenaeus vannamei, and hence the condition for introduction the new species to the shrimp culture industry provided. In accordance for development of shrimp culture, I.F.R.O carried out various research activities, and established specific research center for this purpose. Research projects regarding brood-stock management, breeding and larvae production, grow out, water management and disease identification and treatment have been carried, and induced considerable impacts on quantitative and qualitative development of shrimp culture in the country.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Tuna catches in Indian Ocean have been increasing since 1980 by entrance of purse seiners into the area, making their catches about 10 times over 25 past years. Remarkable catches of the fish, especially the three main species yellowfin, Thunnus albacares, bigeye tuna, Thunnus obesus, and skipjack, Katsuwonus pelamis, have raised concern about overfishing of the stock. In spite of the importance of the valuable fish, the present stock assessment methods are not able to estimate population dynamics parameters precisely in order to understand the real status of tuna stock, and for this, presently precautious strategies are used for fishing management. In 1991, scientific committee of IOTC agreed that conducting a planned tagging project in large scale is the most effectiveness tool for obtaining the required data to improve the stock assessment of the main tuna species. The whole plan of the tuna tagging in Indian Ocean have been developed in two phases: pilot study in small scale, and the main tagging phase in large scale. After achieving successful results from the pilot study in 2001, executive programme for tagging operations in large scale known as Regional Tuna Tagging project-Indian Ocean (RTTP-IO)were developed for over two and a half years for the three main species above. The project was started in May 2005 and ended in 3 rd September 2007. The study area included Seychelles, Mozambique Channel, Kenya, Tanzania waters and Arabian Sea (Yemen and Oman). The objectives of the RTTO-IO were: To tag at least 80,000 fish from the main species, to achieve remarkable recovery (at least 15%), to study migration routs between different areas and stock structure, and to estimate growth rates. The present report was resulted from participation of research cruise for tuna tagging in Oman Sea waters in the line with RTTP-IO. In this cruise, 2835 of yellowfin were captured by two pole and line vessels using live bait, and then released after tagging. Different tagging techniques used in the project were: Conventional tagging (Single and double tagging), Otolith tagging, electronic tagging. The required descriptions for different sections including live bait fishing, capturing of tuna by pole and line fishing and different tagging techniques are given. Since the beginning of the RTTP-IO until the end of executive period around 170,000 tagged tuna from the main target species were released in Indian Ocean, which up to now 9.8% of the fish were recovered. The number of 8 tagged yellowfin was also recovered by Chabahar local fishermen in Oman Sea which the data in details are presented. Moreover, more information on the recoveries obtained from the RTTP-IO up to July 2007 is given.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Sampling was done using 90 post larvae which were produced by reproduction of some broodstock of Penaeus indicus in one day and reared in the same situation for 4 month. Samples were classified in 3 group high growth, medium and low (according to their weight and length). Genomic DNA was extracted from a 1cm 2 piece of muscle using the phenol-chloroform method. . The polymerase chain reaction (PCR) was done using 21 RAPD loci. And PCR products were separated on 3% Agarose gels. From 21 studied loci, 12 produced polymorphic bands. The most polymorphic band produced using OPAQ 9 and the least by OPAQ 7. According to Nei 1972, the highest distance (0.457) was between low growth group and medium and the lowest (0.091) between high growth group and medium, therefore the highest identity (0.912) was between high growth group and medium and the lowest (0.633) between low growth group and medium. Consensus neighbour-joining tree using Nei (1972 and 1978) resulted in two clades, the first including high and medium growth groups and the second low growth group, it appears that low growth group are depended on separated population of the two others. With considering of mean weight of F1(16.25±1.5), mean weight of 15 ±1.2 and mean weight of parent 31.6, response to selection (R) and heritability for growth in this species were estimated 1.2±0.2 and 0.07±0.01 respectively. In another part of this study Sequencing of specific bands and primer design were done and examining of them on the same age specimens is necessary in following.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to study of Hydrology, Hydrobiology and Environmental pollution in the southern of the Caspian Sea. 33 stations were selected from Astara (in the west) to Hassan gholi (in the east). In each station, sampling carried out at suface, 10, 20, 50 and 100m depths. Sampling was performed during four seasons in 2004. Sampling stations also divided into three regions, including west (from Astara to Ramsar), middle (from Ramsar to Sari) and east (from Amirabad to Hassengholi). The results showed that maximum of water temperature was 24 ?C at summer in the eastern region while the minimum of water temperature in winter season has been 9.7 ?c in the western region. The average of dissolved oxygen concontration in spring , summer, autumn and winter season were 6.9 , 6.6, 8.2 and 6.7 mg/l , respectively. The maximum value of salinity was observed in spring (13.55 ppt) and minmum value in winter (8.24 ppt). The average of total nitrogen concentration were season was 711, 884, 583 and 669 µg/l in spring, summer, autumn and winter repectively. The average of total phosphorus concentration was 27.9, 37.1 and 42.6 µg/l in spring, summer, autumn and winter, respectively. The average of dissolved silica concentration has varied from 167 to 923.6 µg/l. The five phyla of phytoplankton have identified. The most species diversity was observed in winter (70 species) but the low species diversity has obtained in autumn. The most phytoplankton population was in spring and summer seasons. The cell abundance of phytoplankton in different seasons was fluctuated from 3914*103 to 17337*103 ind/m3. In addition, the phytoplankton biomas was varied 11.4 to 30.7 mg/l. Result showed that distribution of phytoplankton are varied at different areas, layer, seasons and stations. The average of cell abundance of zooplanktons was varied from 2023 to 12364 and/m3 at different seasons. The mean of bioman varied from 9.40 to 43.48 mg/l. In this study, 17 species and one class of macrobenthos were identified. The frequency of Annelida and Biovalvia was higher than all macrobenthos. The average of macrobenthos was 1218±851 ind/m2 and their biomass was 14±15 g/l. The average of PAHs concentration were 0.038, 0.028 and 0.21 mg/l in spring , summer and winter, respectively. The decending trend of heavy metal concentration were Fe〉Mn〉Zn〉Cr〉Pb〉Co〉Cd〉Cu at different seasons in 2004. The maximum concentrations of organochlorine pesticides (OCPs) components in three regions of sampling zone such as , -BHC (middle), β,-BHC (middle), DDT (east) and dieldrin (east part) were 88.3, 35.0, 74.3 & 79.1 g/l during spring, summer, fall and winter, respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In the early 1980s, an alien ctenophore Mnemiopsis leidyi already known as a gelatinous zooplankton was transported (likely via ballast waters) to the Caspian Sea from its introduced or native water of the Black Sea or western Atlantic which caused negative impacts. In this report, distribution of M. Leidyi, planktonic and benthic organisms had been investigated in the Southern Caspian Sea (Iranian waters) in 6 transects consisted of Lisar, Anzali, Sefidroud, Nowshar, Babolsar and Amirabad at 26 stations during 2005 and 2006. As a whole, 1422 samples had been analyzed included 258, 346, 217, 117 and 484 belong to ctenophore, phytoplankton, zooplankton, benthos and physicochemical parameters, respectively. M. leidyi abundance and biomass were fluctuated between 284-2751 ind.m-2 and 16.9- 390/9 g.m-2 respectively in 2005 while maximum abundance and biomass were recorded in autumn and minimum in winter. In 2006, its mean abundance and biomass were 184-2150 ind.m-2 and 9.1- 209/8 g.m-2 respectively. M. leidyi maximum abundance and biomass were noted in summer and its minimum in spring and winter months. Overall, mean M. leidyi abundance and biomass were recorded in 20 m within 1202 ind.m-2 and 139.5 g.m-2 respectively. Maximum size of the ctenophore was recorded as 55 and 60 mm in 2005 to 2006 respectively, while less than 10 mm length frequency consisted 83.16 and 82.88 per cent of total population. Spatial_ temporal distribution of M. leidyi in different regions showed it was more abundant in west and east (714- 4494 ind.m-2) ratio to central parts (13-1519 ind.m-2) of the Southern Caspian Sea. Species composition of zooplankton had shown the negatively impacts of M. leidyi invasion in the period of sampling as the only 17 holozooplankton were determined with Rotatoria (7 species), Copepoda (4 species), Ciliophora (4 species) and cladocera (1 species). Zooplankton maximum abundance and biomass were recorded in February (3039742901 ind.m-3 and 372.1575.4 mg.m-3) respectively and its minimum were in July (12211601 ind.m-3 and 6.9 7.7 mg.m-3) respectively which was belonged to Rotatoria (76%) and Copepoda (10%). In this study, 21 species of benthic fauna were deter minted belong to Pseudocumidae (5 species), Gamaridae (4 species) Amphartidae (3 species), Oligochaeta, Balanidae, Xantidae, Nereidae, Scarbicularidae, Carididae, Mytilidae, Herpobdellidae and Chironomidae (each 1 species). Oligocheata was recorded with maximum abundance (943 2502 ind.m-2) and Bivalve consisted of higher biomass (68.7162.5 g.m-2). From five phyla of phytoplankton, 172 species were determined of Chrysophyta (74 species), Cyanophyta (32 species), Chlorophyta (32 species), Pyrrophyta (24 species) and Euglenophyta (13 species), in which Chrysophyta and Pyrrophyta were noted as main groups with their maximum abundance (up to 65%) and biomass (93%). Maximum water temperature was recorded in August with 29.52.5 ֯C and minimum in February by 9.31.3 ֯C. Mean value of salinity was 12.010.90 ppt, secchi disk ranged between 0.2 to 7 m and oxygen demand varied from 5.95 to 10.54 mg. l-1). Moreover, silicate concentration was recorded between 200 to 300μg.L-1, Phosphate measured from 31-47 μg.L-1, Ammonia varied in 10-29 μg.L-1, Nitrite (0.6- 1.7 μg.L-1), Nitrate (0.06-4.20 μg.L-1, which mainly accumulated in coastal waters of the southern Caspian Sea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In this study, the identification of the Acanthopagrus species sparidae family by morphometric characters & meristic counts and the stock separation of Arabian yellowfin Seabream Acanthopagrus arabicus as the dominant species was determined through geometric morphometric and otolith shape in the west and east part of the Hormuz Strait. Samples were collected in five different areas including Khuzestan, Bushehr and Hormuzgan Waters as the west of Hormuz Strait, and Jask, Sirik and Sistan & Baluchistan Waters as the east of Hormuz Strait from June 2014 to May 2016. A. arabicus, A. berda and A.bifasciatus were identified in the northern part of the Persian Gulf & Oman Sea, the first one was identified as a dominant species of this genus. Evaluation of morphometric characters and meristic counts indicated that individuals of A.arabicus in west and east of Strait of Hormuz were significantly different from each other which showed high phenotype diversity of this species (p〈0.05) and 85.3% of the individuals were categorized precisely in the correct classification which indicated as a key identification method in the region. From the meristic counts, the number of scales on the lateral line, on the chin and scales from the fifth and ninth spine of dorsal fin to the lateral line were the factors of separation in the west and east of the Strait of Hormuz. Body shape analysis was showed the most variations on the head and top of the body and significant differences of body shape was proved between west and east of Strait of Hormuz. Landmark positions in the samples of the west Strait of Hormuz were indicated the decrease of body depth, increase of head length, leaning of base and pectoral and ventral fin to upper part of the body, meanwhile it was contrary in the samples of the east. CVA correlation analysis showed a significant difference in body shape in the west and east of the Strait of Hormuz (P 〈0.0001). The otolith shape indices showed a significant difference in the west and east of the Strait of Hormuz and the results of the DFA analysis confirmed the accuracy of the classification of the species in the main region by 53.8 percent and the otolith shape in the west of the Hormuz Strait was more circular and had an equal growth in the length and width, while in the eastern part of the Strait of Hormuz they had more growth and aspect ratio, ellipticity and form factor of the area were approved. The results of the otolith Fourier Shape Analysis showed that six components out of the 77 main components were expressed 90 percent of the cumulative strength and difference, and the most changes were observed in the dorsal and ventral part of the otoliths of the Arabian yellowfin Seabream, and 70.4% of individuals of this species were correctly classified in the west and east of the Strait of Hormuz. The results of this survey confirmed two separate stocks in the west and east of Strait of Hormuz.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This survey was carried out by R/V Guilan ship with a conical plankton net of 100 micron mesh by vertical hauls at different stations and depths of 5, 10, 20, 50 and 100m in 4 seasons of 2010. In this study, 16 species formed the zooplankton community including 4 species of Copepoda, 4 species of Rotatoria, 2 species of Protozoa and 4 species of Cladocera from Holoplanktons and 2 species of Balanus sp and Lamellibranchiata larvae from Meroplanktons. The annual results revealed that the mean abundance of zooplankton were 5477±5815 ind/m^3 and 5131±7908 ind/m^3 in spring and winter respectively which were more than other seasons but the maximum biomass (64/58±124/61mg/m^3 ) was in winter that was affected by Rotatoria. The maximum mean abundance and biomass of Copepoda were in summer that were 2830±2342 ind/m^3 and 22/52±21/78 mg/m3 ,respectively and the abundance decreased gradually since autumn and reached the least in winter but increased since spring. The mean maximum abundance of Cladocera (115 ±142 ind/m3 ) was in spring but it decreased gradually, so it reached to less than 3 ind/m^3 in summer. The Protozoa constituted the least community and biomass of zooplankton in southern area of Caspian Sea. The zooplankton community also was affected by Meroplanktons including Cirripedia and Lamellibranchiata Larvae in spring and the Rotatoria contributed highly in zooplankton community in winter with the mean abundance and biomass of 2604±5876 ind/m3 and 50/71±115/33 mg/m3 respectively.Other than Copepoda, other planktonic groups contributed lowly in zooplankton community. It was observed the reduction trend in abundance from surface depths to deeper depths in whole of the year. There were 75% of zooplankton community in stations with 5 to 20m depth and 25% of community in stations with 50 to 100m depth in spring, and also 74% in stations with 5 to 20m depth and 26% in stations with 50 to 100m depth in summer, 73% in stations with 5 to 20m depth and 27% in stations with 50 to 100m depth in autumn and 85% in stations with 5 to 20m depth and 15% in stations with 50 to 100m depth in winter. In analysis of different area of southern basin of Caspian sea the maximum abundance was observed in west in spring, summer and winter which were 7514±8115 ind/m^3 , 3909±2609 ind/m^3 and 8129±11587 ind/m^3 , respectively. There was 2283±2134 ind/m^3 in center area in autumn. The annual statistical analysis revealed that, there was significant difference in total zooplankton community between sampling stations, depths and layers in whole of the year.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Microalgae are very important organisms in aquaculture as major live food in all growth stages of bivalve, some larval stages of crustaceans, some fishes and zooplankton. In this project with the knowledge that local algae can provide better nutritional balance for local animals. Microalgae were isolated, identified and evaluated for their nutritional value for the first time. Another aim of this research was to create phytoplankton culture collection (Bank) in the south of Iran. Water was sampled from Sistan and Baluchesta coasts, then isolation and purification process were carried out in the lab. Species identification was based on morphology and molecular analyses. rDNA was extracted from each strain after PCR, partial LSU-rDNA region were sequenced and compared with similar sequence from GenBank. In order to evaluate their nutritional properties, relative growth, fatty acids composition, total lipid, carbohydrate, protein, and some vitamins of each purified strain were determined. Totally 25 species were isolated and purified, of which 12 strains were recorded in GenBank and their nutritional potential were assessed. Dunaliella cf. bardawill, Isochrysis sp. Cheatoceros sp. Clorella cf. volgaris, Ochromonas sp. and Synechococcus sp. were the most important species research. Iranian strains were varied in their amount of protein (27-40%), carbohydrate (221%) and lipid (8-13%). Proportions of SFA (21-48%), MUFA (33-53%) and PUFA (11-28.5%) were varied among strains. The highest amount of PUFA and B3 vitamin were found in Clorella cf. vulgaris. The highest content of protein (40.12% dry wt) and total fatty acid concentration (30% dry wt) were found in Dunaliella cf. bardawil and Ochromonas strain(CHPO2) respectively. The maximum content of total fatty acid was observed in Ochromonas sp. (CHPO2). Cheatoceros sp. had higher relative growth rate in comparison with other species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was performed to investigating of distribution, biomass estimation and preparing Atlas of seaweeds in coastal and Islands of Persian Gulf (Hormozgan province). The samples were collected monthly from Sep. 2001 to Aug. 2002 in 6 stations of coastal and from Nov. 2002 to Oct. 2003 in 4 stations of Island of Persian Gulf in periods of low tides. For assessment of biodiversity and biomass estimation, the samples were taken from quadrate with 0.25 m2 (0.5m x 0.5m) area in random fashion. During this study 78 species of 4 divisions of seaweeds were identified. Rhodophyta represented 38 species, Chlorophyta 21 species followed by 18 species of Phaeophyta and only 1 species of Cyanophyta. The maximum and minimum species diversity of seaweeds was 74 and 31 species and related to Lark Island (St8) and Michail (St.5) respectively. Although some species such as Gracilaria corticala, Gelidiella acerosa, Laurencia snyderia, Colpomenia sinousa, Padina australis and Dictyosphaeria covernosa were abundant-in-all-stations-during-period-of-this-studynbut-some-species-were-not-see in some stations, such as Turbinaria conoiedes only in Lark island (St.8), Spatoglassum variable and Sleochospermum marginatum in Lark (St.8) and Qeshem islands (Sty), Codium papilatum and Ulva spp. in Lark and Hormoz (St. 7) islands, and Sargassum ilicifolium in Bandar Lengeh (St.2), Shiyo (St.6), Larak (St.8) and Qeshem islands (St. 10). The maximum and minimum value of algal biomass (wet weight) was recorded in Bandar Leneeh (St.2) with 1058 qr.m-2 and Oeshem islands (St.9) with 391 gr.nf2 and there was significant difference between two stations (P〈O.05). Also, the maximum algal biomass was recorded in summer (1466 gr.m- ) and in Tahoneh-Gorzeh (St.4) and minimum value (130 grem-2 ) in Qeshem islands (St.9). In the other hand, the maximum value of algal biomass was recorded in Brown algae division (824 gr.m2) and in Bandar Lengeh (St.2) and the minimum value of algal biomass were recorded in Green algae division (26gr.m-2) and in Hormoz (St. 7) and Qeshem islands (St.9). The average biomass of red algae, brown algae and green algae was 559 gr.m-2 (St.4), 1160 gr.m-2 (St.2) and 519 gr.Bf2 (St.4) respectively. The maximum and minimum length of two brown algae Sargassum sp. and Padina sp. were recorded in spring (growth season) and summer respectively. Also the maximum and minimum dry on wet weight percent were recorded in summer and spring and therefor, there is significant difference between different seasons (P〈O.05).

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Population structure of 2 species in the Caspian Sea (Rutilus frissi kutum and Rutilus rutilus) and 2 species in the Persian Gulf and Oman sea (Rachycentron canadum and Polynemus Plebeius) were studid by using Microsatellite and RAPD. For this 210 specimens of kutum (Khoshkrud, Tonekabon, Gorganrud, Anzali Lagoon and Kura River mouth), 90 (Gorgan Bay, Anzali Lagoon and Volga), 184 of cobia (Bushehr, daier, Bandarabass, bazm, lengeh) and 235 of (Khozestan, Bushehr , Bandarabass and chabahar ) were sampled. Genomic DNA was extracted of fin using the phenol-chloroform .The quantity and quality of DNA from samples were assessed by spectrophptometer and 1% agarose gel electro-phoresis. PCR was carried out using microsatellite and RAPD primers. PCR products were separated on 8% polyacrylamide gels that were stained using silver nitrate. Result showed that in kutum the average of expected and observed heterozygosity was 0.54 and 0.49 respectively. Significant deviations from Hardy-Weinberg expectations were observed almost in all of location (P≤0.01, (P≤0.05) ). Using Fst and Rst there was significant difference between locations (P≤0.01). In study of Rutilus rutiluscaspicu the average of expected and observed heterozygosity was 0.5 and 0.7 respectively. Fst didn t show significant difference between iranian locations (P≥ 0.01) but it was Significant between Iranian populations and Russian populations (P≤ 0.05). In cobia the average of expected and observed heterozygosity was 0.655 and 0.874 respectively. Significant deviations from Hardy-Weinberg expectations were observed almost in all of location (P ≤0.01, (P≤ 0.05)). Significant differences (P〈0.01) weren't observed between Rst recorded in the same region specimens studied but were observed between Rst recorded in the different region specimens studied. In Polynemus Plebeius, the observed heterozygosity in the Bushehr and Bandar abbas (0.22±0.13) was the highest and in Chabahar the lowest (0.171±0.175).Key word: Population Genetic, Rutilus frissi kutum ,Rutilus rutilus, Caspian Sea, Rachycentron canadum , Polynemus Plebeius, Persian Gulf and Oman sea.

Description: Iranian Fisheries Science Research Institute

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Description: In this stydy, 486 species belonging to 12 divisions were classified in the Caspian Sea (Iranian waters). The number of dominant species in each division and the percentage of that, such as Baccillariophyta, Chlorophyta, Cyanophyta, Pyrrophyta and Euglenophyta were 186 (38%), 110(22%), 80 (16%), 71 (14%) and 28( 6%) respectively. There were a few numbers of Exanthophyta, Chrysophyta, Haptophyta, small felaggelate Ochrophyta and Cryphophyta divisions. The numbers of these divisions’ species are ranged from 1-5 species. In Baccillariophyta, genera such as Navicula ( 24 species,15%), Nitzchia (22 species, 13%) and Chaetoceros (18 species, 11%) were dominante. In Chlorophyta division, The highest number of taxa belonged to Oocystis genus, which included 10 species and comprasing 16% of predominant species. Scenedesmus genus is in the second place ( 9 species , 14% ). In cyanophyta, the number of species, Anabaena, Oscillatoria and Microcystis are 9, 9 and 7, and 17%, 17% and 13 %t of all predominant species. In Pyrrophyta division , Peridinium genus with 11 species and 22% is dominant. Glenodinium and Gymnodinium are ranked after Peridinium. From Euglenophyta, genera such as Euglena and Trachelomonas are dominant. The number of species are 13 and 11species respectively. Euglena genus comprising 50% of all dominant species. A total of 807 phytoplankton species were distinguished in inland waters ecosystems. 284 species(36%) Baccilariophyta, 241 species (30%) Cholorophyta, 144 species (18%) Cyanophyta, 59 species (7%) Euglenophyta, 57(7%) pyrrophyta are listed in database. Divisions of Chrysophyta, Exantophyta and Cryptophyta have a few numbers (6,4 and 3 respectively).from Baccilariophyta, Nitzchia and Navicula with 45 and 28 species make up 23% and 14% of the dominant species, respectively. In chlorophyta division,genera such as Scenedesmus ( 30 species ranking first place among dominant species) and Cosmarium (18 species second place) are distinguished. From Cyanophyta, genera Phormidium, Chrococcus, Oscillatoria and Lyngbia have the highest number of species that have 13, 12, 9 and 9 species respectively. These species accounted for 12%, 11%, 9% and 9% of dominant species respectively. In Euglenophyta, Euglena and Trachelomonas are dominated. These genera accounted for 21 species (39%) and 15 species ( 28%) of dominant species. From Pyrrophyta, Peridinium genus is dominant which includes 30% of dominant species. Gymnodinium with 8 species ( 17%) is in the second place.

Description: Iranian Fisheries Science Research Institute

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Description: Certain diseases such as bacterial , viruses, parasirtic ones Protozoa parasites Could severe mortalities in farmed shrimp last decades. Two hundred and seventy Fenneropenaeus indicus with the weight of 10± 1.09 were selected , transferred to Iran Shrimp Center and distributed to the treatmentsand controls (3 groups). The objective of our study was to describe the signs, prevalence, and intensity of Hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Syndrom Virus (WSSV) shrimp as group A and non-infected non- exposure to WSSV shrimp as group C in triplicate. The results revealed that Hemocytosis associated with a decrease in hyalinocytes and large-granulocytes (less than 8%) and a considerable increase in semi-granulocytes in group A and B in contrast to group C that Hyalinocyte increased. This infection made the shrimp susceptible to WSSV disease. It is believed that Hemocytosis causes a condition which can result in a rapid mortality among susceptible species, F. indicus when exposure to WSSV is occurred.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Primary researches on Persian Gulf Shrimp Propagation conducted at 1984 in Boushehr Research Center. Peneaus semisulcatus propagated for first time in Iran, in laboratory scale. In the later years, other endemic species such as P.merguiensis, Metapenaeus affinis and penaeus indicus candidated for aquaculture, and various researches on propagation and PL production conducted. Various research projects carried out in southern research centers have been considered in this report, and results of propagation for each species and methods of PL production have been involved. By the results, it is possible to propagate all above mentioned species in controlled condition, and by development of hatcheries, PL requirement for shrimp farms could be provided.

Description: Iranian Fisheries Science Research Institute

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Description: Monitoring and studing ,qualityand quntity fingerling some of species such in this as (Rutilus rutilus , Cyprinus carpio ,Rutilus frisii kutum ) were accomplished in 2000-2001. in this poroject some of chemical and physical factors in ponds, and growth of fingerlings were studied ,from introducing stage to ponds , till releasing into GOLESTAN province rivers. Some chemical and physical parametres were measured such as O2,NO2,PO4 In 10 days interval. The growth of fingers were studied by measuring percision1mm and 0.1mg . length of frequency was determined in different class interval untill 3 gr . condition factor was calculated for all species. Number of fingerlings released into river estimated by sampling ( 3 times in each hauling ) .number of KUTUM andKORA fingerlings that they have released in 2001 , %14-%19 had increased in comparison with 2000. While mean weight of fingerlings in 2000 was higher than in 2001 . but about common carp in 2001, either mean weight or number was better than in 2000 . in during time of releasing with decreasing of level of water in ponds , temprature of environment increased and that caused water quality fell down so fast . present of different aquatic plants , undessired fingerlings fish and larve of frog are some difficults in ponds.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This feasibility study carried out in response to request of Tehran Province Fisheries Management, in order to Qanat Salehabad water assessment for aquaculture relataed purposes in Tehran Municipality – Regions 19 & 20. During 6 monthes between May – October 2011 in 3 sampling stations by 3 replicates investigations executed. Physic-chemical, Heavy methals, organochloro-organophosphore pesticides and microbial nominated parameters analysed in reference to Iran national and international standards as well, resulted an overall assessment on quality and quantity of Qanat water for aquaculture purposes. Results expressed station 1 in all studied seasons can be develop for fisheries warmwater applications and in cold seasons (September-March) fisheries coldwater relevant activities can be consider. Stations No 2 & 3 known as infeasible for fisheries applications but would be recommend for agriculture rrigation. According to the results, possibility of developing live fish shopping centers (Cold – warmwatetr fishes) between station 1 and before crossing the Qanat flow and Behesht-e-Zahra freeway is considerable, indeed water rights could be negotiate between Tehran Municipality and Tehran province Fisheries Bureo. Also application of feasible anti microbial filters and other minimum limitation parameters control is necessary before leading Qanat water flow into proposed live fish shop. And suitable septic tank is recommended at the outlet of proposed fisheries units. Monitoring of nominated physic-chemical & microbial parameters during operation of fisheries unites highly recommended.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Pin bone removing is a new equipment for pin-bone removal increase customer satisfaction and revenue even further the bones were pulled out at good speed. Pin boning especially is very often done manually which causes lots of global transports to low cost countries for processing new technique will help make processing operations more efficient and profitable The new generation pin bone removing equipment is mainly for trout and is not suitable for carp fishes the new pin bone remover which works with air of compressor and hand. has proved to work in almost twenty different fish species, including Great silver smelt, Pike Perch, Coho, Sockeye salmon, Atlantic Salmon, Sea Trout, Saithe, Haddock, Herring, Whitefish, Chinook, Salmon, Perch, Rainbow Trout, Char, Mackerel and Hake. Therefore it will be suitable for carp fishes too. After a day’s work all vital parts can be removed by hand without any tools, for effective cleaning to the fish and gives nice looking filets and is determined to help processors by offering state of the art bone-removing equipment which will increase speed, yield and efficiency. But pin bone removing done manually does not give a nice looking fillet also has a considerable waste of fish.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: A survey on Parasitic infections on endemic fishes of North of west Azarbaijan province were done during July 2001 Oct 2002. Altogether 267 specimens from 11 different fish species were examined. The fishes were collected from seven stations of Aras, Zangbar and Ghotor Rivers. The fishes were transported alive to the laboratory where they were meaursed and weighted. Then they were killed by cutting the spinal cord.. According to the results of this study 28 different parasites were revealed, 23 of them were detected to the species level and 5 of them to the genus level. The metazoans are as follows : The monogeneans, are Dactylogyrus extensus , D. chramuli, D. lenkorani, D. kendalanicus, Silurodiscoides siluri, Diplozoon megan and Gyrodoctilus sp. Two digeneans :Diplostomum spathaceum , Allocreadium ispoporum. Three Cestods: Ligula intestinalis, Digrama sp. and Caryophylleous laticeps. Two nematods: Rhabdochona hellichi, Eustrongylides excisus and only one acanthocephalus, Neoechinorhynchus rutili . The Protozoans are: Ichtyophthirius multifilis, Trichodina perforata, Chilodonella sp., Amphileptus branchiarum, Apiosoma sp/. Vorticella sp/. Myxobolus musayevi, M. cristatus. And two protozans from freshwater fishes: Stylonchia pustulata. and Arcella vulgaris. According the results of this study five new parasites and six new hosts for Iranian. Freshwater fishes were introduced. Diplozoon megan from: Abramis brama, Carassius carassius, Capoeta capoeta, Cyprinus carpio, Rutilus rutilus and Alburnoides bipunctatus. Trichodina perforata from Lucioperca lucioperca, Capoeta capoeta, Alburnoides bipunctatus, Barbus capito and Silurus glanis. Myxobolus cristatus, Tetrahymena pyriformis and Amphileptus branchiarum from Capoeta capoeta. Finally , Stylonchia pustulata and Arcella vulgaris for the first time were recoreded from freshwaters of Iran.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Coregonus lavaretus is one the important species of coregonidae family. It has adipose fin and the tail fin has a deep notch. The fish is special for fresh and cold north waters and are mainly found in north hemisphere and north of Europe, Asia and America. It feeds aquatic invertebrate and zooplanktons. The mentioned species has tasteful meat and supplies the major part of fish consumption. The big fishes meat is tasteful, low fat and has a cucumber like odorous characteristics and is mostly consumed in dried and smoked forms. Since this fish is important in inland fisheries and is releasable and developing in water sources of lakes behind dams has been considered and respect to purity of water resources behind dams. In the lake of Karaj dam has been considered as the only habitat and considered to have to low biological information from October to late march 2001 for 6 months totally 82 samples were caught and studied and through this way lack of fishing in the region by determining 11 stations, the samples were collected and grown, relation between length and weight, age and length, propagation, nutrition and fertility parameters were evaluated. Most of caught fishes are 4 to 5 year olds and 3 and 4 year olds. The 4 year olds males were majority and 5 and 6 year olds females formed the dominant group. Correlation relation between age and total length in both sexes (male and female) was very significant at 1% level, of course in females the correlation was around 100%. The weight and total length in males and females that was caught both had high correlation at 1%level; of course this correlation in females had been very stronger than males. % level related to gonad weight and sexual evolution stages of females. Gonad had correlation at 5 The most absolute fecundity had belonged to 5 year old female with 19120 ovules and the lowest to 4 year old breeder. With 11496 ovules the most relative fecundity was 62.13% and belonged to 5 year old breeder and the lowest was 36.06% belonged to 4 year old breeder by increasing the quantity of ovules in breeders the sixth stage of sexual evolution the fecundity rate has been directly increased as well. % of Gonad weight was the most effective factor on absolute fecundity and according to (R2) more than 90 variations of breeders; absolute fecundity is claimable by their gonad weight. Among all traits, affecting gonad weight fecundity the breeders sexual evolution stage has been recognized the most effective factor on fish ovules diameter. Generally in oligotrophic aquatic resources they become mature before the age of 3 and in utroph aquatic resources and full of edible zooplanktons they become mature with 2 years of age. In respect to nutrition this fish eats a broad range of zooplanktons separated from surface to bed of water of dam lake and no kind of selection was seen there so that in different ecological condition it can differ appropriately to kind of foods available in different seasons so that in cold months (December to March) the food diet of this fish varies toward eating water bed living things such as tobifex.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to determine of phytoplankton abundance and diversity of water and their spatial and temporal fluctuations in the Mazandaran coastal of Caspian Sea in 6 months, at 4 transects (Tonekabon, Nowshahr, Babolsar, Amirabad) during different season of 2012-2013. 72 samples were collected at surface layer of water in 5, 15 and 30m depths. The sampls were analyzed based on the standard methods. 112 species contributed in phytoplankton community structure which where classified in 9 phyla namly: Bacillariophyta (42 species), Pyrrophyta (18 species), Cyanophyta (14 species), Chlorophyta (15 species), Euglenophyta (11 species), Cryptophyta (2 species), Chrysophyta (3 species), Haptophyta (1 species) and Xantophyta (1 species). Meanwhile small flagellate algae with Maximum Linear Dimension (MLD) 〈10 µ observed which they classified in small flagellates. Mean annual phytoplankton abundance with standard error obtained 164 ± 32 million Cells/m3. Seasonal study showed that phytoplankon abndance of summer was 1.5 folds of spring. The value in auttuman was same as spring, however it increased sharply in winter. The mean phytoplankton abundance of winter was 5 folds of the other seasons. Mean phytoplankton abundance of Tonkabon and Nowshahr (west transects) were 1.6 and 2 folds of Amirabad (east transect), respectively. Bacillariophyta with 89 percent of total abundance was the predominant phylum and Pyrrophyta was the second one. The third and fourth of dominant phyla were Cyanophyta and Chlorophyta, respectively. Chrysophyta and small flagellates showed equal percentage of abundance (1.4 percent of total abundance). Monthly study showed that Chaetoceros throndsenii was the first dominant species in Ordibehesht, Tir and Shahrivar. However, the first dominant species in Aban, Day and Esfand were Thalassionema nitzschioides, Skeletonema costatum and Pseudonitzschia seratia respectively. Mean phytoplankton biomass calculated 156.5 ± 18.1 during the study period. The mean of biomass was higher in summer and winter than the two other seasons. Phytoplankton biomass was formed mainly by Bacillariophyta and Pyrrophyta in all seasons. The highest biomass were belonged to Cyclotella meneghiniana and Coscinodiscus jonesianus (Bacillariophyta) in spring and summer respectively.While in fall and winter Prorocentrum proximum was in the first place of dominat species. Small size and flagellates species of different phyla (Chrysophyta, Bacillariophyt...) had importance role for determination of ecological and water quality conditions during spring to autuman. The increasing of phytoplankton abundance within these times indicates to regeneration of nutrients or entrainment of nutrient-rich deep water. Dominant species were observed in single forms, small filament and loose colonies during spring to autuman. However, these form shifted to fair-long chains form in winter which it indicates to nutrient-rich water was brought to the surface by vertical mixing process. It seems that environmental stress and instability of ecosystem was benefit to Chaetoceros throndsenii and Pseudonitzschia seriata which are known as species with bloom potential. Ability of reproduction in sewage environment (Chaetoceros throndsenii) and toxin production (Pseudonitzschia seriata) are the ecological and physiological significant characters of the two species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Fish soup powder is produced from three kinds of fish in Chabahar catched in Oman sea white flesh of many low value fish like threadfin bream, perches etc. can be used to prepare instant fish soup powder. This product has a high consumer acceptability in many countries in this research project we used 4 low value fishes with local name of Sarm, Chaman, Aroos and Talal belonging to families Carangidea, Lethrinidea , Drepanidae and Scombroidea. soup powder packed in laminated pouches and the shelf life of it is one year in ambient temperatures. The quality used to be good up to 6 month but after this time the quality decreases gradually. The experiment on fish soup showed that the percentage of protein was 32 percent, fat 11 percent and humidity 12 percent and ash 4 percent. The experiment conducted on fish soup powder was peroxide, total microbial count, organoleptic test for 6 month.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Commercial catch data in Khouzestan Province (North West Persian Gulf) landing centers were used in this study. Total number of active fishing dows and boats in 2004 were 601 and 1440 respectively. Abadan area head the maximum number of boats (%49) of the province in this year and it was calculated %70 for dows. A total catch of 19950 tons was landed in 2004 which showed 40 percent decrement comparing to previous year in which 33508 tons had been recorded. This decrement occurred after a series of increments in catch during the last years. 71 Percent of total catch of the province belonged to Abadan area. Total catch of Silver pomfret (Pampus argenteus) in 2002 was 182 tons and increased to 408 tons in 2004. L∞, condition factor (K), M. and E. were estimated for this species 32.62 cm, 0.54 per year, 1.06 and 0.64 respectively. Total catch of Tiger tooth croaker (Otolithes rubber) increased from 942 tons in 2002 to 2300 tons in 2004. L∞, K, M. and E. were estimated for this fish 62.12 cm, 0.5 per year, 0.92 and 0.6 respectively. Tenualosa ilisha had a landing of 6841 T in 2003 and showed a steep decrement (%82) in 2004. Abadan Landing places had the most percentage of catch for O. rubber (%72) and T. ilisha (more than %82). Less economically important species landings were increased during last years. Catch statistics of Lizard fish Saurida tumbil in 1993 was 81 T and reached to 318 T in 2004 and for Thread fin bream Nemipterus Japonicus it increased from 80 to 972 tons.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: For gathering of data on biometry and catch statistics, 5 mobile working group in the regions of Anzali, Kiashahr , Noshahr , Babolsar and Torkmen were involved from the start ( 2004/10/23 ) to the end of fishing season (2005/04/10 ). Gathered data were entered to the computer based on species. For data analysis different methods were undertaken such as Bertallanfy equation for growth parameter Beverton and Holt for total mortality rates, pauly's imperical model for natural mortality rate and cohort Analysis for biomass estimation. Total catch with taking into account of poaching, was estimated 15140 tons reported by scientific committee of catch and fishing harbor relief of Iranian Fishery Company. The total recorded catch of beach seine cooperatives was 10644 tons that comprise 70.2% of the total catch. Total catch of Caspian Kutum was estimated 6612 tons that had 1856 tons (22%) decrease with comparison of the last year. Biomass of Caspian kutum in Iranian coastal Water of the Caspian Sea in 2003-2004 estimated about 20400 tons. The total catch of golden gray mullet was estimated 4425.6 tons that constitute 29.2% of total catch of bony fishes. Biomass of this species was estimated 12200 tons and based on this amount of biomass, Maximum Sustainable Yield (MSY) was calculated as 3577 tons. Long nose mullet comprised only 1.4% of the mullet catch. The catch of common carp in 2004-2005 fishing season continued its increasing trend and caught in amount of 3087 tons by beach seine cooperatives that had 2.2 fold increase in comparison of previous fishing season. Most of the catch occurred in Golestan province and was immature and undersize and the length frequency modes showed moving towards bigger size and older age groups in comparison of previous fishing season. The catch of pick perch in 2003-2004 decreased with comparison of the last year and estimated about 22.5 tons. Most of the catch of pick perch was immature and undersize. Approximately all of the catch is due to releasing of fingerling of this species by Fishery Company especially in Guilan province. Total catch of bream was estimated 27.4 tons and most of which was immature and undersize. Population of asp is critically endangered and the catch of this species is very low (500 kg). Restocking of this species must be considered by Iranian fishing company.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was determined some important extracted materials, Such as : Alginates of sodium & calcium and alginic acid in three species of brown seaweeds (Sargassum illicifolium , Cystoseira indica and Nizimuddinia zanardinii) during in different season in 1387 in coastal erea of Sistan and Baluochestan Provience. At First seaweeds sample were colected from sea beach and transferd to lab, so washed in fresh water and dried in the sun. So were under Alkaloid extraction with sodium carbonate (5 Persent), that were obtined fluid after filteration. The sodium alginate fibers extracted from fluid were combined by Alcohol. The calacium alginate fibers were deposited by calacium chlorids (5%) and Alginic acid resulted with chloridric acid (1N). The extraction yield of sodium alginate, calacium alginate and alginic acid in different samples of seaweeds with following Explaination: The presentage we have got, can be said , the present average of sodium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 28.4±2 , 28.9±2 , 27.2±1.9 present, the present average of sodium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 19.7±1.4, 18.6±1.4, 19.2±1.3 present and the present average of sodium alginate at Nizimuddinia in Spring were 23.4±1.5, 23.8±1.5 , 20.6±1.5 in these three areas. The present average of calacium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 33.7±0.5 , 33.7±0.8 , 33.1±1 present, the present average of calacium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 28.8±1.5, 27.4±1, 27.4±1.5 present and the present average of calacium alginate at Nizimuddinia in Spring were 35.9±2.8, 23.9±1.5 , 35.9±2.8 in these three areas. The present average of Alginic acid at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 19.2±1.6 , 25.6±1.7 , 18.4±1.1 present, the present average of Alginic acid at Cystoseira in Spring in three areas at above mentioned goes in this way: 16.8±1.2, 15.8±1.1, 16.4±1 present and the present average of Alginic acid at Nizimuddinia in Spring were 19.2±1.6, 19.6±1.7 , 18.4±1.5 in these three areas. The present average of sodium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 20.8±1 , 21±1 , 27.8±1.9 present, the present average of sodium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 13.2±1, 14.1±0.7 present and the present average of sodium alginate at Nizimuddinia in Summer were 25.3±2.3, 16.7±2.1 , 15.3±1.3 in these three areas. The present average of calacium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ): 29.5±2.1 , 29.7±2 , 28.3±2.2 present, the present average of calacium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 20.2±0.9, 20±0.7 present and the present average of calacium alginate at Nizimuddinia in Summer were 25.3±2.3, 29.7±2 , 23.1±1.5 in these three areas. The present average of Alginic acid at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ): 19.5±0.7 , 19.7±0.6 , 18.7±0.7 present, the present average of Alginic acid at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 11.5±1.4, 11.1±1.5 present and the present average of Alginic acid at Nizimuddinia in Summer were 14.8±1.3, 15.3±1.3 , 14.6±1 in these three areas. The present average of sodium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 31.5±4.3 , 31.6±4.8 , 29.4±4.8 present, the present average of sodium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 23±2.7, 21.4±2.8, 21.4±2.7 present and the present average of sodium alginate at Nizimuddinia in Autumn were 25.8±2.2, 26.4±2.1 , 23.4±1.9 in these three areas. The present average of calacium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar ): 43.3±1.6 , 42.8±2.7 , 41.7±2.9 present, the present average of calacium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 34.3±5.9, 32.4±5.5, 30.9±5.2 present and the present average of calacium alginate at Nizimuddinia in Autumn were 50±7.1, 50.2±7.5 , 45.6±5.2 in these three areas. The present average of Alginic acid at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar ): 28±3.8 , 28±3.5 , 26±3.9 present, the present average of Alginic acid at Cystoseira in Autumn in three areas at above mentioned goes in this way: 20.2±1.8, 19.2±2.1, 18.7±1.6 present and the present average of Alginic acid at Nizimuddinia in Autumn were 25.9±3, 26.1±2.9 , 24.6±3.6 in these three areas. The present average of sodium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 35±1.6, 35.2±1.8 , 33.8±1.9 present, the present average of sodium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 24.1±2, 22.9±1.2, 23.3±0.7 present and the present average of sodium alginate at Nizimuddinia in Winter were 28.2±1.3, 29.2±1.7 , 26.2±1.8 in these three areas. The present average of calacium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar ): 45.6±1.9 , 45±2 , 44.6±2.5 present, the present average of calacium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 38.2±2.8, 35.9±3.4, 35.6±3.5 present and the present average of calacium alginate at Nizimuddinia in Winter were 56.4±3.5, 56±3.5 , 51.8±2.5 in these three areas. The present average of Alginic acid at Sargassum in winter season in three areas (Chabahar, Tang and Pasabandar ): 31.8±1.2 , 32.1±0.9 , 30.9±1.2 present, the present average of Alginic acid at Cystoseira in Winter in three areas at above mentioned goes in this way: 21.1±1.2, 20.4±1.4, 19.3±1.4 present and the present average of Alginic acid at Nizimuddinia in Winter were 26.4±2.1, 27±2.2 , 24.6±2.2 in these three areas. The more amount of calacium alginate in Autumn can be found at Nizimuddinia in Tang area , wite amount of 50.2 present and the lates amount of Alginic acid at Cystoseira can be found in Summer with 11.1 present. If the Alginic acid become more than 20 present , it can be economical , that it can be showed more than 20 present in Autumn and Winter . According to this results, One way ANOVA showed that average of Alginates were not similar and significant differences ( P〈 0.05 ) between species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Sturgeons are valuable species of the Caspian Sea and the Persian sturgeon Acipenser persicus is an endemic species of the south Caspian Sea. Possessing the highest breeding percentage in sturgeon hatcheries and catch numbers of this species is of particular importance in Iran regarding rehabilitation of stocks and fingerling release. The main aim of this study was to understand the histological structure of this valuable species and to make use of this information in other basic and applied research. The present study was conducted from 1998 through 2001 on a total of 34 A. persicus specimens (18 male and 16 female) with an average weight of 13.75 kg and an average length of 146.3 cm. Fishes under study were caught using gillnets and beach seine in the fishery zones 1 and 2. Biometric measurements of fish specimens were carried out on catch and primary gonadal staging was assessed by means of macroscopic examination. Tissue samples including gill (primary and secondary ray), male and female gonads (anterior, central and posterior), digestive tract (oesophagus, stomach and intestine), liver (different parts of the large and small lobules) and kidney (anterior, central and posterior) measuring a few millimeters in thickness and a few millimeters in width were prepared and fixed individually in buoin solution. Tissue samples were dehydrated in different dilutions of alcohol and chloroform solution until they were clear. They were then embedded in paraffin and sectioned at 5-7 µm using a Leitz microtome and stained following the hematoxylin and eosin (H & E) method. Distinct samples were photographed using a light microcopy in 10 microscopic fields. The microscopic examination of gills revealed the presence of chloride cells, secondary cartilage, erythrocytes, gill lamella, epitheleum, polar cells, respiratory cells and respiratory spines. Ovary in A. persicus was similar in structure with that of other sturgeons and a major part of it was comprised of stroma tissues. Follicles were the main structural and functional indices in ovary. The main distinguishing feature of the ovary in the early stage of growth is the presence of ovarian grooves in gonads. Grooves were observed in stage I of sexual maturity and oogonia were seen in the primary growth of protoplasmic ovary. They are attached densely to the dorsal part of the abdominal cavity and their development is slow. Oocyte cells are observed assembled in ovary sacs. In the II stage of maturity gonads are yellow to pink in color with distinct lateral folds. Fat tissue is the main energy source for oocytes. Duration of this stage is very long. Primary oocytes are single layered, follicle shaped and could be observed with naked eyes as white spots in an ovary section. The III stage of maturity is characterized with trophoplasmic growth of oocytes, reduction in fat reserves, increase in oocyte volume, appearance of pigments under cell membranes and growth of arc cilia on gelatinous part of follicles. Nucleus is located away from the center and surrounded by several nucleoli. The granulosa layer occurs at this stage. In the IV stage of maturity oocytes growth is increased and nucleus is seen migrating toward the animal pole. Tiny yolk granules are observed in the animal pole and larger yolk granules and fat droplets in the vegetative pole. Growth of external layers of oocyte continues while protoplasmic growth is completed at this stage. At this stage germinal vesicle reaches its final growth and oocyte is covered with follicle, jelly coat, zona radiata external, zona radiata internal, fatty layer and pigments. The stage V of maturity is characterized with intensive bleeding in ovaries, residues of non-ovulated oocytes, appearance of stage II oocytes and decomposition of residues of non-ovulated oocytes. In the stage VI of maturity oocytes resembling stage II of maturity are clearly observed. Ruptured follicles, pigment reserves and oocytes representing various maturity stages are also present. Testis in Persian sturgeon in the first stage of maturity includes spermatogony cells in its germinal parts. Male gonads are situated along notochord and attached to the dorsal diaphragm or primary area of kidney formation through short mesenchymes. In stage II of male gonad maturity, spermatogonia cells are situated in gonad channel walls in a single layer without wrinkles. Primary and secondary spermatocytes and sertoli cells are present. In stage III of male gonad maturity, spermatogony cells intensively divide and reproduce. Blood vessels are observed in the form of condensed blood cells. Development and formation of primary and secondary spermatocytes, meiotic divisions and presence of spermatids are characteristics of this stage. Stage IV is distinguished by an active cycle of spermatogenesis, further meiotic divisions and entrance of sperms into the sperm channels. In stage V intensive bleeding occurs in testis. Spermatozoids, sperm channels and numerous blood vessels are also observed in this stage. In stage IV of male gonad maturity large epithelial cells of follicle and emptied sperm channels are observed. Kidney in Persian sturgeon is comprised of three sections; anterior (pronepherose including malpighian bodies and nephritic segments) middle and posterior parts. Nephrons include malpighian bodies, glomerula, Buman capsule, renal tubes or urinary tubes, neck part, spiral section, middle part, distant spiral section and shows various patterns in microscopic histological studies. Liver tissue is composed of hepatocytes, biliary tubes, lymphatic vessels, liver capillaries or sinusoid, kupfer cells and liver cells. Liver cells are roughly circular and polygonal in structure with a single and distinct nucleus and single nucleolus. Endoplasmic reticulum and other essential cell organs are present in its cytoplasm and considerable fat and glycogen reserves are observed in cell cytoplasm. Therefore large numbers of theses vacuoles are observed in a stained liver cells. Pancreas in Persian sturgeon is comprised endocrine and exocrine parts. The exocrine part releases pancreatic excretions and the endocrine section (Langerhans islands) releases hormones. The digestive system is comprised of alimentary canal that includes the mouth, pharynx, esophagus and intestine with epithelial and muscular layers. The epithelial layer sustains epithelial coat, parenchyma, condensed layer, muscular epithelium, sub- epithelial layer, muscular layer including ring and longitudinal layer and serum membrane. The pharynx epithelium is stratified in anterior part and simple in posterior part. Epithelial cells of pharynx include mucus like matrix in cytoplasm. These cells lack muscular epithelium and a complex and condensed connecting tissue is present beneath the epithelium. Stomach has two independent sections of glandular structure for collecting food and commencement of chemical digestion and non-glandular structure with strong muscular layer specified to create stomach movement and mechanical digestion. Epithelial parts are simple or wrinkled in structure. Intestine stretches from the pyloric section of stomach to the anal part and includes duodenum, anterior and posterior intestine and rectum. Duodenum is a part of intestine where biliary canals, pancreas and pyloric ceaca enter. Intestinal epithelium is comprised of a single layer of stretched columnar cells that encompasses epithelial cells. Rectum is the last section of intestine and a sphincter separates it from posterior intestine. Anal is the terminal opening of the alimentary canal. Goblet shaped epithelium cells are observed in epithelial structure of rectum while simple tubular glands without large intestinal branched glands are present in Parin. Results obtained in this study along with characterizing microscopic structure of studied organs in Persian sturgeon facilitated implementation of other related specific researches.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: For the first time in research program and species diversity the white legged shrimp (Litopenaeus vannamei) was introduced in Iran in 2005 by Iranian Fisheries Research Organization. In two recent years two projects was carried out successfully in Hele Research Station and according of these projects study of growth, survival rate and final harvesting of Litopenaeus vannamei impact of 25, 35 and 50 (per/m2) stocking densities was done .Nine 0.4 ha earthen ponds were stocked from 26 June 2006 to 11 November 2006 and the rate of stocking was 100000,140000 and 200000 Pl in 25, 35 and 50 per /m2 respectively. We also carried out daily test of pH, temperature and dissolve oxygen two times per day but transparency, depth and salinity one time per day. The mean weight in 120 days was 17/84±2/6, 16/03±1/9 and14/09±2/3 grams in 25, 35 and 50 per /m2 respectively and highest weight was seen in 25 density. Results demonstrated that there was a significant relation (P〈0.05) between mean weight. The yield were 1745, 2055 and 2425 kg/pond although 4/3, 5/1 and 6 ton/ha in 25, 35 and 50 per /m3 respectively. The maximum and minimum survival rate 97/96 and 88/46 was seen in 25 and 50 per/m2. The minimum FCR 1.43 was seen in 25 per /m2. For economical calculating just by using the cost of larva and feeding indicates that the highest benefit percentage was seen in 25 density. The average of salinity, pH, temperature and dissolve oxygen were 36.1-37 ppt, 8.1, 30.2-30.6 degrees centigrade and 5.9- 6.4 mg/l.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Caspian Sea with an area of 400 thousand square kilometers is the largest lake in the world. The Caspian Sea about 1200 km from north to south on the longest section and an average width of 320 km. Along the coastline around the Caspian Sea is about 6500 kilometers. Caspian Sea is about 78,000 cubic kilometers of water volume that is 44% of stocks of blue lakes around the world. Caspian Sea basin, which is composed of seven major basins of the main watershed from west to east are: juniper, Talsh- Anzali, large Sefidrood between Haraz Sefid and, Hraz- Gharehsou, Nagorno Sv- Gorgan and Atrak in the basin of Aras no limits to the beach. Aras sub-basin is located in the North West and Iran, the second largest sub-basin of the Caspian Sea. Talysh-Anzali on the Caspian Sea basin West and the seventh largest sub-basin of the Caspian Sea. White basin is located in the South East of the Caspian Sea and the extent of the sub-basin of the Caspian Sea. Haraz located in the south Caspian Sea basin and the ninth largest sub-basin of the Caspian Sea. Gorgan is located in the South East of the Caspian Sea basin and the fourth largest sub-basin of the Caspian Sea. In these areas, about 28 percent of the total fish production in the northern waters of aquatic allocated.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The grey mullet is referred as the scientific name "Mugil cephalus" mugil cephalusis most important of species culture of many areas in the world. To obtainable of the procedure culture in the climate of our county (Iran), we executed a project of fingerlings mugil cephalus from 1994 to 1991 Where as our county was lack of fry, therefore, we purchased 2000 specimens of fry from Hong Kong. In order to we imported fry and they were transported into shaheed Marjani farm, by chiller track we provided a house green and then fries enteredinto fiber glas tankes that the dimension of fiber glass tanks were 2* I *2m but the conceret ponds were 3* I *2m during the manitenance of fry in tanks, in addition toremoving of stress due to transportation quarantine, the manual feeding offered torice bean plus wheat flour and also zoea of live food (Daphani plus Artemia) fry adapted to fresh water (1ppt) and estuary (14ppt) and then, they moved into the earth ponds. The species determinant and biometry were occurred to 30specimens of fry during the maintenance of fry in tanks, the climate condition for temperature and submitting of ponds the earth ponds were limed. And water flow, we were fertilized to chemical and animal fertilizers, there are full of these materials. After the earth ponds were obtained them. The earth ponds have high produced natural production. The experiment was performed on fry with hapas, the survey of survival and the final introduction of fry were transferred in to the earth ponds. This pond included in 5000 specimens. This pond has 4000areas in adjacent Ghomishan basin and also 1000 fry have placed in the earth ponds. These areas have 1000 m2 in Marjani farm, the average weight of fry was approximately 0/5 (gr) and the average Length was 0/4 (cm). During the maintenance of fry, fry were fed by live food and detritus that were achieved by fertilizer. Fry fed with supplement food, this function occurred by food container. The feeding times were fed 2 or 3 times in every day, the amount of food was based on dry weight, fry that calculated during culture. During culture, the control of physical and biological factors in the ponds were recorded and a few fish were captured. They performed on biometry the summery results achieved as follows: In the freshwater pond the average weights of valuable period in the end of culture included in 235 (y) and 668 (gr) respectively. The average lengths of fry (fork length) in the valuable period in the end of culture at first and second years contain 26 Cm, 37* I Cm respectively. In the saline water pond the average weights of fry in the valuable period in the end of culture are first and second respectively. The average lengths of fry in the valuable period in the end of culture are first and second years contain 26 cm and 35.2 cm respectively.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Barcodes are short segments of DNA that can be used to uniquely identify an unknown specimen to species, particularly when diagnostic morphological features are absent. These sequences could offer a new forensic tool in plant and animal Conservation-especially for endangered species. It was proved that a small fragment of mitochondrial DNA from the 5′-end of cytochrome c oxidase subunit 1 (COI) gene as a reliable, quick and costeffective identification system for most Crustacea like shrimp. Take a look in DNA barcoding website show that there is a little data about Iranian shrimps which live in Persian Gulf and Oman Sea. In this Study six species of shrimp: Fenoro penaeus indicus, Fenoro penaeus merguensis, penaeus semisulcatus, Metapenaeus affinis, Marsupenaeus japonicas, Fenoro penaeus penicillatus were collected from different stations in Persian Gulf and Oman Sea. All materials were preserved in 70% ethanol and were shipped to the laboratory for taxonomic studies. After identification, the total DNA was extracted; COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. The results were amazing and the interesting part was that analytical methods for showing species relationship suggested that Fenoro penaeus penicillatus is closer to Marsupenaeus gnus than penaeus gnus. This finding needs more investigation to be proved. We suggest a workflow for DNA barcoding, including database generation and management, which will ultimately be necessary if we are to succeed to join universal DNA barcode for Crustacea.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In This study formaldehyde concetration were examined in 5 species of persian Gulf by schryer test . These speciese were "Silver pomfret" (pampus argenus) " brushteeth lizard fish " , (saurdia tubil), "Barred spanish Macerel " (sconbermurus commerson)" sardinella longiceps " and Green tiger shrimp (penaeus semisulcatus) , the rate of formaldehyde concen tration determined in three different temperture storage as 0c , - 20c. -40c. 1 – The results indicated formaldehyde in the Muscle of fish. 2 – The level of formaldeyde concentration obtained in this species have been compared and discussed. 3 – The Flucation in forma dehyde concentration in this species dose not show the same pattern. 4 – Using the schryr test the shelf life for each species were calculated. The longest shelf life were found to be belong to sliver pomfret and shortest to be belong to lizard fish . T.V.N. test in this study as a subsidary contrul test.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Chrysiptera unimaculata, an algivorous species also living on the coral reef flat and being territorial but not considered as a strict farmer in this location. Maximum length is 8 cm in Persian Gulf. It is living in close association with macrophytes. Adults are found solitarily or in small groups among coastal algal reefs, rubble or over open beach-rock of reef flats exposed to moderate surge and feeds mainly on benthic algae. C. unimaculata is oviparous, distinct pairing during breeding. Eggs are demersal and adhere to the substrate. Males guard and aerate the eggs.

Description: Electric rays, thought to be the most primitive of the skates and rays, have stout tails but have rather expansive disc. This group is distinguished by the presence of powerful electric organs, derived from branchial muscles in head region. Torpedo sinuspersici found inshore in sandy bottoms, and well offshore from the surf zone down to 200 m. Also on or near coral reefs (like Kish Island in Persian Gulf). Common in shallow sandy areas. Occasionally hooked by anglers, more often seen by divers; can deliver a strong shock. Flesh is edible. T. sinuspersici can survive for hours after being stranded on the beach. Little is known of the life history of the Gulf torpedo. It is a sluggish predator of bony fishes. At night it actively hunts for food, sculling slowly through the water about a meter above the bottom; during the day it usually rests on the bottom and opportunistically ambushes unwary prey. It uses its broad pectoral fins to envelop the target fish before delivering an electric shock to stun it. Usually solitary, they may form groups during the mating season. Reproduction is a placental viviparous, with the developing embryos initially surviving on their yolk sacs, and then on enriched uterine fluid produced by the mother. Litters of 9-22 young are birthed in the summer. Newborns measure about 10 cm wide; males mature at a disc width of 39 cm and females at 45 cm.

Description: Boloceroides spp. are looking like an untidy mop, this anemone is sometimes seen in sea grass areas on many of our shores. It is possibly seasonal. Sometimes, large numbers are seen (up to 10-20 animals in a trip) and then none at all.  Tiny swimming anemones may sometimes be confused with Sea grass anemones which have translucent tentacles with tiny spots. The swimming anemone harbors symbiotic single-celled algae (zooxanthellae). The algae undergo photosynthesis to produce food from sunlight. The food produced is shared with the sea anemone, which in return provides the algae with shelter and minerals. The oral disk and tentacle muscles are used to obtain, retain, and ingest prey; in Boloceroides spp. tentacles can autotomize if it is needed to evade a predator. Tentacles can control body form by use of their endodermal muscles. Retractors are longitudinal muscles that will aid in withdrawing tentacles and the oral disk if they are exposed to the open air.  This hypothesis is furthered because in comparison to other sea anemones, Boloceroides is loosely attached to its respective substrate, thus allowing the pedal disk to detach quickly resulting in a rapid swimming response. Boloceroides can reproduce both sexually and asexually. As Anthozoans, Boloceroides produce sexually by bypassing the medusa life cycle stage; this allows Boloceroides (and all Anthozoans) to release their egg and sperm creating planula a bilaterally symmetrical, flattened, ciliated, motile larva.

Description: Many aquarists know this fish as Ambylgobius hectori but accepted name is actually Koumansetta hectori. K. hectori, Hector's goby, is a species of goby native to the Indian Ocean (including the Red Sea), first record from Persian Gulf (Kish Island, Iran) to the islands of Micronesia in the western Pacific Ocean. It can be found on sheltered coral reefs at depths of from 3 to 30 meters (though usually between 5 to 20 meters). This species reaches a length of 8.5 centimeters (3.3 in) SL. This attractive little fish measures only 2” at maturity and spends its time hovering over rocks and substrates out in the open water column. Known for their bold yellow stripes, peaceful disposition, the diminutive Hector’s Goby is a nano reef favorite. It can also be found in the aquarium trade.

Description: Pempheris vanicolensis has reported for the first time from Persian Gulf (Kish Island). Various aspects of the biology of P. vanicolensis Cuvier & Valenciennes, a recent Lessepsian (Suez Canal) immigrant into the Mediterranean, are given. Red Sea and Mediterranean populations were compared, and the results indicate that the spawning season is shortened in the Mediterranean, continuing from April to September, as opposed to year-round in the Red Sea. Descriptions of a ripe ovary and post-larva from the Mediterranean are given. Individuals reach a mean size of 10.8 cm in their first year, and 14.4 cm in the second year. Little change has occurred in the diet of the immigrant population, and both populations feed nocturnally, chiefly on larval and adult stages of planktonic crustaceans. Direct underwater observations on the diurnal behavior of the species show that the fish leave their daytime cave shelter at sunset, congregate at a nearby site and then migrate inshore and disperse into small groups to feed. Before dawn, they reassemble at the cave's entrance, and finally enter it at sunrise, after the school has built up. Observations on Persian Gulf sweepers show that the fish are segregated into size groups, ranging 15–18 cm adults at depths of 3 m.

Description: The silver pomfret, Pampus argenteus, is distributed in Indo-western pacific ocean, especially in Persian Gulf and Oman sea, and although is widely commercially exploited, the genetics structure of this species is not clear completely. In the present study, the genetic diversity and population structure of Pampus argentus in the Persian Gulf and Oman Sea were investigated using microsatellite DNA markers. Genomic DNA from 125 specimens was extracted and PCR amplification performed. Seven loci with reasonable polymorphism were amplified. The results showed that average of observed heterozygosity was ranged from 0.54 while expected heterozygosity was 0.67. After applying the Hardy-Weinberg Equilibrium (HWE) test, some loci were found to be significantly deviated from HWE in some populations in which deficiency of heterozygotes was apparent. Population differentiation observed among all populations (P〈0.01). The population differentiation (Fst) value between Kuwait and bushehr was the highest and significant (0.087) and between Chabahar and Bushehr (0.021) was the lowest and significant. Genetic distance analysis showed that the largest distance (0.269) was between Khuzestan and Chabahar and the smallest distance (0.075) was between Bushehr and Chabahar. The reported results could be of interest for management and conservation programmes of this species in the Persian Gulf and Oman Sea.

Description: During the years 1382-83, some biological aspects of three species Tiger-toothed Croaker, Otolithes ruber, Javelin grunter, Pomadasys kaakan, Black Pomfret, Parastromateus niger, have been investigated in the Oman Sea along Sistan-O-Baluchistan province. Based on the frequency of developed maturity stages (IV, V), a prologed spawning activity extending from August to March was determined for O.ruber, and the Gonadosomatic Index (GSI) suggests two peaks:the minor one in October and the major one in March-April. Comparison of Hepatosomatic Index (HI) and Condition factor (Kn) with the peak spawning indicated a reverse relationship. According to length at first maturity (Lm50%= 40cm) and exploitation of fish at length 42cm above, the stretched optimum mesh size of nets was calculated to be 100mm. On analysis of stomach food item, fish group dominated (80%), which of them Anchovy accounted for 40% of the total frequency. Comparison of mean length between the years 1374-75 and 1382-83 showed 4cm in decrees. Population dynamics parameters were determined through length based modals and the exploitation rate was estimated to be E=0.6, suggesting high level exploitation. The usal streched mesh size of nets(constructed from multifilament webbing) for capturing O.ruber were less than the proposed optimum mesh size of nets, indicating 78% of the total landings were smaller than Lm50%. Total landings in 1381 have increased up to 15% when compared to those of the year 1376. Despite the decrease of total efforts (Panel.day) by 37%, the mean value CPUE has increased up to 82%. Regarding Pomadasys kaakan species, a year-round spawning activity has been observed, with two peaks: A major one in April and an aminor one in September. As those of O.ruber species, there was an inverse relationship between HI and Kn with the peak spawning activity. Based on the length at first maturity (Lm50% = 44cm) and capturing fish of the length at 46cm and above, the stretched optimum mesh size of nets was calculated to be 149.6mm. According to analysis of stomach contents, fish group accounted for 35% of the groups studied, which of them , Largehead hairtail, Trichiurus lepturus linnaeus, constituted 52% of the total frequency. In addition to estimation of population dynamics, the exploitation rate was estimated to be E= 0.49. Majority of the species catch was made by mesh size of nets used for O.ruber of which 133mm mesh size accounted for the level (30%). Information on length distribution showed that 60% of the total fish landed was below Lm50%. Total landings in 1381 decreased to 10% in comparison to 1376.Total fishing efforts and CPUE also decreased to 33% and 24% respectively. For parastromateus Niger, There was a long period spawning activity from March to December, with two peaks: A major one in August and a minor one in March. According to Lm50% (39cm) and catching of fish 39cm above, the stretched optimum mesh size of nets was calculated as 167.7mm. Based on stomach contents, ring-shaped worms and larval shrimp equally made 29% of the total frequency showing the highest contribution. Comparison of mean length indicated 3cm in decrease in 1382-83 when compared to 1374-75. Population dynamics parameters were estimated and the exploitation rate was estimated to be E = 0.54. The usual stretched mesh size of nets for P. niger was 146mm and 81% of the total fish landed were smaller than Lm50%.In 1381,total catch of the species increased four and a half-fold in comparison to 1376, while total efforts decreased up to 28% and CPUE increased two and a half-fold.

Description: A survey on parasitic infections on Barboid fishes of South- west of Iran (Khouzestan province) were done during Spring 2002 to Autumn 2004. Altogether 296 specimens from 5 different fish species were examined. The fishes were collected from five stations of Karoun River (Ahwaz-Goulestan, Molasani, Dez Dam), Karkheh River (Hamidia Dam) and Shadgan Lagoon. The fishes transported alive to the laboratory where they measured and weighted. Then they were killed by cutting the spinal cord. According to the results of this study 22 different parasites were revealed, 15 of then detected to the species level and 7 of them to the genus level. The metazoans are as follows: The monogenea, are Dactylogyrus onchoratus, D.persicus. Two digeneans: Diplostomum spathaceum, Asymphilodora sp. Five nematods: Rhabdochona denudata, Philometra karuensis, Philometra sp., Psudocapilaria tomentosa Contracecum larvae sp. and only one acanthocephalus, Neoechinorhynchus. Three protozoans are: Ichtyophthirius multifiliis, Trichodina sp., Goussia sp., Balantidium sp. And Myxozoans: Myxobolus persicus M. karuni M. nodulointestinalis, M iranicus, M.mesopotamia, M. pfief eri, Myxobolus sp., Myxidium rhodei, Myxidium pfiefferi. Histopathological investigation of some parasites was done as well. Pathologacal potential of M. nodulointestinalis and M. pfiefferi were revealed. According the results of this study three new parasites and eleven new hosts for Iranian freshwater fishes and three new host for three Myzoxoans were introduced.

Description: The aim of this research was to identify fish species distributed in the water resources located at West- Azarbaijan province. Sampling was done seasonally, from 14 sampling sites in main branches of different rivers including Aras, Zangmar, and Ghator. Fish samples caught with different tools, fixed in 10 % formalin, and finally transferred to the laboratory for biological investigations. The identified fish were 25 species belonging to 8 fish family comprising of Cyprinidae, Balitoridae, Cobitidae, Siluridae, percidae, poecilliidae, Gobiidae, Salmonidae. Among them, cyprinidae family had the highest percentage of diversity. There were 2 species from Balitoridae and 1 species from the rest of other family.

Description: Hamoun fish, Schizothorax zarudnyi, is an indigenous species of the eastern waters of Iran, which is exclusively found in this region. However recently drought occurrence in the Chahnimeh reservoirs (a semi natural water body) making them vulnerable to extinction. As an appropriate action to address this problem and according to the 3 side contract between Italian Government – UNDP - Islamic republic of Iran Government and the Italian government financial support to reduce poverty in the Province of Sistan-Baluchestan through the reinforcement and dissemination of aquaculture activities the project was developed at the Hamoun Research Unit by the Chahbahar Fisheries Research Center to record the breeding normative of 1 g weight larvae for restocking and other researching purposes. 331 broodstocks of the indigenous species Shizothorax zarudnyi weighing 800-2450 g were collected from the Chahnimeh reservoirs in early autumn, 2006. From 5/3/2007 (the project is supported and communicated on 2010) , Ovulation was stimulated with three stimulators; pituitary extract (3-6 mg kg-1 body weight), GnRH-A (20-30 mg kg-1 body weight) and anti dopamine (10-15 mg kg-1 body weight) that was given in 2-3 doses to breeders. Of 169 injected breeders , some were injected On mid March of 2007 (12-13 ºC water temperature) responded to the injection 25% ,while the rest were injected On April of 2008 (14-16 ºC water temperature) responded to the injection 65%. In the present project of 167 breeders 82 were female and 87 male. Totally 30 female breeders released their eggs in different stages. 20 female breeders released their eggs completely, 3 breeders released half of their eggs and 7 released 1/3 of their eggs. The male breeders just injected in the final dose of hormontrapy and all were ready for releasing sperms however the ovulation in female breeders occurs between 353-428 h ºC and after the final dose of injection. Ripe eggs were stripped from the females and fertilization was done by the dry method. Fertilized eggs were transferred to veis incubators and troughs. Incubation period for eggs differs and larvae hatch out after about 910 days at an average water temperature of 12.5 °C. Maintained at 13-14 °C, complete absorption of yolk sac in Shizothorax zarudnyi larvae occurred after 5-8 days. Larvae were fed with a mixture of powdered milk and egg yolk in this stage followed by decapsulated Artemia cysts and nauplii of Artemia and then on formulated starter diets used for carps. Because the ponds were not ready, larvae were maintained in troughs for about ten days before they were transferred to two 1200 m2 earthen ponds where they reached a body weight of about 1 g. They were then handed over to the Iranian Fisheries department in the region. Larvae were fed with the starter feed SFCO in the earthen ponds. About 350 thousand larvae were stocked in two earthen ponds. Based on the results of present study and other studies we may conclude that artificial breeding in Schizothorax can be successfully achieved at 14-16 ºC in flow through systems using hormone therapy (combination of GnRha and anti dopamine) and larvae could be easily cultured in earthen ponds. However this species exhibits lower growth rates as compared to carps its high expenses could have an important role in economical feasible.

Description: This study was carried out in August – september 2009 in Bushehr province waters. Two different shrimp bottom trawls were used in which were woven by Poly Amid and Poly Ethylene with mesh sizes (stretched) of 50 mm and sack with 35 mm. Also 2 different vessels of two dhows and one steel ship each with 26 and 30 hauls were used and taken in to comparison from point of obtained results. Total catch of PA and PE trawels were 4592 and 5535 kg of which the ratio of shrimp catch and by catch for PA trawl was 32.4 and 67.6 % respectively; and for PE trawl was 34.2 and 65.8%. Total catch of PA and PE trawls of steel trawler ( ship ) were estimated 3158.0 and 4784.7 kg, respectively, of which the shrimp/ bycatch ratio was 6.6 : 93.4%. There was a higher amount of bycatch for PE comparing to PA trawl (p〈0.05). on the other hand the decrease of shrimp and bycatch for PA comparing to PE dhow trawlers were 21.3 and 15.0% and for ship were 34.6 and 34.0% respectively. The weight ratio is 1:15 i.e . in PE ship trawl, in proportion to on kg shrimp, 15 kg bycatch was harvested. it is proposed to standardize the mesh size and also to do obligtory the instal of squared mesh size panel .

Description: 30000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (2007 and 2008) and transferred to the earthen ponds in Astaneh Fisheries Research Station (Gilan) and Sheiban Resaerch center (Khozestan), In order to assess the viability of rearing fry up to 1 gram fingerlings under the climatic condition of north and south part of Iran . Growth parameters from the larval stage to 1 g were studied also. The fry with 300 mg weight, were released in to 3ponds.specimens were separated to tree experimental categories. fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured . Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings.Catla was gain 1 g. during 15 day while Roho and Merigal were gain 1 g. during 14 and 10 days respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility specialy in other provinces like Sistan and Baluchestan were remind for futhre studies.

Description: Awareness of health and nutrition of fish has led the food industry to development of fishery-based ready-to-eat products. In this study seven filling materials based on 60-70 - 80% fish mince from 7 local fish species ‘i.e.’ Talang Queen fish (Scomberoides commersonnianus), oriental sole (Brachirus orientalis), Barred sickle fish (Drepane longimana), Big eye Croaker (Pennahia anea), Japanese threadfin bream (Nemipterus japonicas), javelin grunter (pomadasys kaakan) and Indian mackerel (Rastrelliger kanagurta) were formulated and applied for developing fish strudels. A 9-point hedonic scale ranging from 1 (extremely dislike) to 9 (extremely like) was used for screening the acceptance of the prototypes. A 3-component D-optimal Mixture Design was also applied to optimize the fish strudel formulation. Preliminary experiments were done to identify the high-impact ingredients, which had a high effect on the sensory quality when their levels (%) were changed. The results indicated that fish strudel containing 60% Talang Queen fish (Scomberoides commersonnianus) mince in the filling materials had significantly higher liking in odor, flavor, and overall acceptability than the other prototypes. So it was selected for quality changes study. Product's characteristics and stability were studied during 6-month storage at -18 °C. The fish strudel packed in a polyethylene sealed bag was stable during the storage period. The peroxide and TVBN values remained low. The results in this paper provide important information for companies planning to develop convenience food from low value fish. Furthermore, development of strudel filled with fish mince/ flesh could be an effective way to enhance nutritional and functional value of such products. The products could be used as a nutritious snack especially for kids which could be a means of increasing fish consumption.

Description: 15000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (200 ) and transferred to the earthen ponds in Astaneh Fisheries Research Station , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 12 ponds. Specimens were separated to tree experimental categories: 1= (30%indian carp and 70% chinees carp) , 2= (50%indian carp and 50% chinees carp) , 3= (70%indian carp and 30% chinees carp) .single chinees carp production were used as control . fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured. Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production ) was 647g for Labeo Rohita ,420 g. for Cirhinus merigala and 734g for Catla catla. The special growth rate (SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for futhre studies.

Description: Fish soup powder is produced from three kinds of fish in Chabahar catched in Oman sea white flesh of many low value fish like threadfin bream, perches etc. can be used to prepare instant fish soup powder. This product has a high consumer acceptability in many countries in this research project we used 4 low value fishes with local name of Sarm, Chaman, Aroos and Talal belonging to families Carangidea, Lethrinidea, Drepanidae and Scombroidea. soup powder packed in laminated pouches and the shelf life of it is one year in ambient temperatures. The quality used to be good up to 6 month but after this time the quality decreases gradually. The experiment on fish soup showed that the percentage of protein was 32 percent, fat 11 percent and humidity 12 percent and ash 4 percent. The experiment conducted on fish soup powder was peroxide, total microbial count, organoleptic test for 6 month.

Description: Regarding to monitor of demersal resources in the Persian Gulf and Oman Sea, and also biomass and CPUA estimation of them, eleven research cruises were carried out by using R/V Ferdows-1 equipped with bottom trawl, covering the area from 49º 00´ E in the west (borderline with Kuwait) to 61º 25´E in the east (borderline with Pakistan) from 2009 to 2011. The study area was stratified into 17 strata (A to Q) of which 10 strata (A to J) were in the Persian Gulf and 7 strata (K to Q) were in the Oman Sea, covering the depths of 10-50 m in the Persian Gulf and 10-100 m in the Oman Sea. A total of 316 stations were randomly selected and the biomass and CPUA were estimated by swept area method. In 2009, due to the bloom of jellyfish, there was some problem for sampling and therefore made some bias in our estimation. Therefore, the biomass of jellyfish was excluded from all calculations. The comparison between two regions indicated that the percentage of density of demersal fishes in the Persian Gulf during years 2009, 2010 and 2011 were 1.5, 3.7 and 1.7 times more than the Oman Sea and totally 60-80% of total biomass was found for the Persian Gulf. Also a comparison among 17 strata the highest biomass was found for K region (Sirik to Jask) in the Oman Sea in 2009 & 2011; and C & D regions (Genaveh to Dayyer) in 2010 in the Persian Gulf. The same comparison was done for CPUA of commercial, non-commercial and total in both water bodies and it was found that in years 2009 and 2011 the regions of L (Jask to Meidani) and K (Sirik to Jask) in the Oman ; and 2010 the G region (Mogham to Farour) in the Persian Gulf had the highest value of this parameter. It can be concluded that the north-west of the Oman Sea has the best condition of biomass and CPUA of commercial and non-commercial demersal fishes; and on the contrary the low values were estimated for A region (north-west of the Persian Gulf) and P region (Konarak to Ramin) in the Oman Sea. With review the mean CPUA in defferent depth layers for years 2009, 2010 and 2011, it was concluded that with increasing the depth, the mean CPUA is decreased and the lowest CPUA belongs to depths of 50-100 m. The comparison between commercial and non-commercial groups in both ecosystems, it concluded that the density of commercial species were higher than non-commercial ones; and for years 2009, 2010 and 2011 the commercial species consist of 52.2, 57.1 and 59.7 % of total biomass. In all years the Persian Gulf indicated higher values than the Oman Sea. The most abundant fishes were Rays, Catfishes, Grunts, Japanese threadfin bream, Carangids, Hair tail, Barracuda and Lizardfish for both Persian Gulf and Oman Sea.

Description: This study was carried out on rivers, basins & qanat of Qom region during the 1376-1378and Fish samples were taken from 2 permanent rivers (Qomrood ,Ghara_chag)& from some seasonal ones (Tagharood, Zavarian, Vesva, Biraghan). Also local fishes was followed in 100 qanats in the region and Random fish sampling was done in permanent river extension out of province. This study aimed to recognize different fish species in the province water reservoir and estimating the fisheries potential in the province. For fish sampling nets such as mashk, salik, hook and hand tailored were used. Electroshoker was not used at all. Results of fish recognition showed that fish samples belong to 12 species & 4 family of "cyprinidae", "Balitoridae", "cyprinodontidae" and "poecilidae".The qanat fishes of the montain (kahak, Khagestan) and pastora area (Ghanavat,jafarabad) included by two families of "cyprinid", "Balitoridea".In some qanat which were connected to rivers more species of fish and anotherAquatic animal were observed & sampled.At the and map of geographical distribution of local fishes was planned.

Description: The project carried out at Shrimp Research station of Bandargah in 2011 to determine the production parameters such as; Average body weight, production, survival and feed conversion ratio in closed- recirculating aquaculture system for shrimp culture. This project was performed in 4 m3 fiberglass tanks. 3 tanks was chosen for the control and 3 were assigned to treatments. Considered Species in this treatment was white shrimp (Litopenaeus vannamei) that with a density of 60 pieces per square meter, and was reared for 90 days. Culture in control as usual and change water regularly, but the treatments did not change water during the period of culture. Water in the treatment tanks was going to turn the filter system and waste was removed by mechanical and coal filter embedded in the route, and after filtering was transferred to the wastewater tank. Wastewater was aerated in this tank and after 24 hours was pumped out and return to treatments tanks. In the final of project, mean weight, survival rate and production for control and treatment, was respectively, 18.27 ± 2.84 and 20.70 ± 1.47 g, 73.83 ± 4.14 and 77.17± 13.66 percent, and 0.81 ± 0.14 and 0.97 ± 0.23 kg/m^2. Statistical analysis results show that there are no significant differences found between treatments and control (P〉 0.05), although in all the above criteria, the relative improvement was achieved to compared of control. But the results of feed conversion ratio was 1.27 ± 0.16 for treatments that are significantly different (P 〈0.05) with controls (1.56 ± 0.23). This result showed that efficiency of the food was better in closed- recirculating shrimp culture system.

Description: A survey on Parasitic infections on endemic fishes of North of west Azarbaijan province were done during July 2001 Oct 2002. Altogether 267 specimens from 11 different fish species were examined. The fishes were collected from seven stations of Aras, Zangbar and Ghotor Rivers. The fishes were transported alive to the laboratory where they were meaursed and weighted. Then they were killed by cutting the spinal cord.. According to the results of this study 28 different parasites were revealed, 23 of them were detected to the species level and 5 of them to the genus level. The metazoans are as follows: The monogeneans, are Dactylogyrus extensus , D. chramuli, D. lenkorani, D. kendalanicus, Silurodiscoides siluri, Diplozoon megan and Gyrodoctilus sp. Two digeneans :Diplostomum spathaceum , Allocreadium ispoporum. Three Cestods: Ligula intestinalis, Digrama sp. and Caryophylleous laticeps. Two nematods: Rhabdochona hellichi, Eustrongylides excisus and only one acanthocephalus, Neoechinorhynchus rutili . The Protozoans are: Ichtyophthirius multifilis, Trichodina perforata, Chilodonella sp., Amphileptus branchiarum, Apiosoma sp/. Vorticella sp/. Myxobolus musayevi, M. cristatus. And two protozans from freshwater fishes: Stylonchia pustulata. and Arcella vulgaris. According the results of this study five new parasites and six new hosts for Iranian. Freshwater fishes were introduced. Diplozoon megan from: Abramis brama, Carassius carassius, Capoeta capoeta, Cyprinus carpio, Rutilus rutilus and Alburnoides bipunctatus. Trichodina perforata from Lucioperca lucioperca, Capoeta capoeta, Alburnoides bipunctatus, Barbus capito and Silurus glanis. Myxobolus cristatus, Tetrahymena pyriformis and Amphileptus branchiarum from Capoeta capoeta. Finally , Stylonchia pustulata and Arcella vulgaris for the first time were recoreded from freshwaters of Iran.

Description: In order to come up with the responsible fishing pattern, there was a need to identify some of the biological characteristics and population dynamic parameters of yellowfin tuna, skipjack and longtail tuna in the Persian Gulf and Oman Sea. Occurrence of empty stomach was high in all the specimens obtained from the gill net fisheries. Purple back flying squid was the most dominant prey species observed in the yellowfin tuna study (61% in males and 57% in females). The length of maturity (Lm50%) for yellowfin tuna was estimated 76 cm, 62.5 cm skipjack and 62.5 cm longtail tuna. Length range of skipjack was from 31-100 cm and bony fishes were the most dominant prey species observed in the skipjack. The monthly gonad somatic index was driven higher values after June which could be indicated as spawning period. The catch per unit of effort of three purse seiners (Azadegan 1, 2, 3) was compared in 1998- 2000. The species composition was 47% yellowfin tuna, 51% long tail tuna and 2% skipjack.

Description: This Research has been done monthly in Sefidroud River and its tributaries from Agust 2003 to July 2004. From the total number of 2027 specimens of caught fish, 800 and 629 were males and females respectively, and 598 specimens were undistinguished. Samples were caught by cast nets. Fork length range of all specimen was 33-255 mm (119.56 ±46) and their weight rang was 0.42-257.5 gr (34.4 ±39.06) gr and their age range was 0-5 (1.76 ±0.959) year. The average of relative frequency in C.c.gracilis was 65.1 % . The average of condition factor was 1.07±0.13. Between Fork length and weight of C.c.gracilis. Was exponential relation and coefficient of correlation was estimated 99.11%. Growth cofficient (k) was 0.114 ; L_∞ was estimated 373.5 mm. C.c.g consumed of Chrysophyta phylum more than other Phytoplankton phyla in Sefidroud river and from Bentic point of view, consumed of clay, Filamentous algae detritus, insects, seeds. Absolute fecundity range was 1823-9274 (4553.8 ±2169.1) eggs. Considering the GSI and frequency percent of maturity stages, spawning period of the fish begins from April and peak of spawning is in June and terminates in July.

Description: The Blooming due to the some species of phytoplanktons especially Dynoflagellates has made some problems for water ecosystems and aquaculture. In this study, the density of phytoplanktons specially Cochlodinium sp. and also environmental factors such as temperature, pH, dissolved Oxygen, and transparency were recorded two weekly in 18 stations of Hormozgan province, Iran in order to monitoring of the possibility of phytoplankton blooming. During six months monitoring, the target phytoplankton, Cochlodinium sp was not observed in shrimp farms. But, other phytoplanktons and zooplanktons were observed as follow: 13 genus of phytoplankton and six genuses of zooplanktons has found in ponds, main water channel and sea. The diatoms with 10 genuses had the highest abundance and Dynoflagellates with 3 genuses had the lowest abundance and blue-green phytoplankton with one genus was in lowest group. Totally, diatoms with 77%, Dynoflagellate with 15% and blue-green alga with 8% abundance were the main populations of planktons in the studied area.

Description: Harmful algal blooms resulting in red discoloration of coastal waters in the Persian Gulf, Iran were first observed in January 2007. The species responsible for the bloom, which was identified as Cochlodinium polykrikoides, coincided with massive aquatic organisms’ mortalities in the Persian Gulf. In order to provide optimum growth and bloom forming, C. polykrikoides cells were sampled during the bloom conditions in the coastal waters of Persian Gulf. After adaptation in filtered seawater, they isolated by positive phototropism characteristic of this species to light. They were grown in modified media culture at different salinity (30, 32 and 35ppt), temperature (20, 23, 26 and 28ºC) and intensity (35, 70 and 90 µmol m^-2 s^-1) with an initial cell density of 50 cell mL^−1. The results of the present study clearly showed that the highest alga biomass was obtained following culture by using A^2 medium under the 32ppt salinity, 26°C temperature, and under a 11h light:13h dark photoperiod regime at a light intensity of 90 µmol m^-2 s^-1 provided by cool white fluorescent tubes. Mean cell density of C. polykrikoides in a 60 liter tank for ten days reached to 32 × 10^6 cell L^−1. Moreover, individual C. polykrikoides chain with 18 cells was observed for the first time in cultures. Based on the results from the present study, as mentioned above, providing suitable media culture and physical condition (light intensity and temperature), bloom forming of C. polykrikoides start from day 8 to 10 and will be continued until day 24 t0 28. In the other hand, C. polykrikoide cells immediately crashed and destroyed.

Description: The project of the Study on fishing and resource management of fisheries within Southern Caspian Sea was conducted on the base of an agreement made between fisheries organization (Shilat) and the Iranian fishery research organization (IFRO) signed in 2010. In this library-based study the current situation of bony fish fisheries and biology of these species has been surveyed in Iranian waters of the Caspian Sea . In this survey internal and external environmental factors of bony fishes activities were considered. Using SWOT analysis method, current situation of fisheries was investigated..One of the most important strategies for conserving anadromous species such as Caspian kutum , common carp, roach, bream and …. is rehabilitation of the natural spawning ground in rivers, coastal lagoon specially Anzali lagoon .By conducting this strategy not only restocks of bony fishes is done ,but also reduced the cost of artificial restocking programs and releasing fingerlings .In short term , it is nessesary to enhance the stocks of the species that have low catch is done by huge releasing of the fingerlings and study the qualification of the program.

Description: Study on Viral Nervouse necrosis (isolation, characterisation and pathogenesis) in Golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) Viral Nervous Necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. For the past two decades, betanodavirus infections that cause Viral Nervous Necrosis (VNN) have emerged as major constraints on the culture and sea ranching of marine fish in almost all parts of the world. More than forty species mainly of marine origin have been so far affected and this number is likely to rise in future following the introduction of new species and the increase of aquaculture trade. Unknown acute mortality occurred in wild golden grey mullet Lisa auratus and Liza saliens in Iranian waters of Caspian Sea in recent years. In order to isolation and confirmation of causative agents of golden grey mullet mortality in the Caspian Sea, a complementary research investigation project was designed in 2005 and approved immediately in Iranian Fisheries Research Organization (IFRO). Many diagnostic aspects such as Virology (Cell culture and Elctereone Microscopy), Hemathology, Bacteriology, Histopathology, Molecular biology (NestedRT-PCR), Heavy metals measuerment and Serology (IFAT and IHC) were employed in mentioned multidiciplinary project. About 322 moribund fish samples which revealed skin darkening, erratic swimming behavior such as spiral and belly-up at rest and high distention of swimming bladder. Suspected samples were collected from coastal capture sites in iranian north proviences in 2006 till 2009. Targets tissue such as brain and eye were removed in strile condition and then kept in -80oC frezzer for cell culture and Nested-RT-PCR. Other tissue samples from liver, kidney, intestine, stomach, gill, skin and muscle, gall bladder and gonads were taken and fixed in 10% buffer formalin and same parts fixed in glutaraldehyde 3% for histopathology, IHC and EM respectively. Cytopathic effect (CPE) was observed in those cell cultures just six days after inoculation with the dilutions of the tested 312 homogenate supernatants. CPE in monolayers of cells cultured (SSN-1 cell line) was characterised by thin or rounded, refractile, granular cells with vacuoles. Nine samples were positive in virology assay. Nested- RT-PCR was done on suspected tissue samples and supernatant of CPE positive samples and 21 tissue samples and all CPE positive samples were positive. IFAT was selected as a confirmatory method for identifying viral strains replicating on cell cultures and carried out with rabbit anti-betanodavirus serum on suspected tissue samples and some smears of CPE positive samples. Some bright points approved betanodavirus antigen and confirmed cell culture and Nested-RT-PCR findings. In fixed tissue samples widespread and massive vacuolation were observed in brain, spinal cord, retina and optical nerve. In order to confirmation of diagnostic findings , IHC was done with monoclonal antibody antibetanodavirus and some red-brown points were observed. Theses findings revealed expected viral antigens and confirmed previous results. Moreover, virus particles with 25-30 nm in diameter were visualized in infected brain and retina using positive staining in TEM. Also pathogenicity test was employed to confirm the obtained results. So Guppy fish Poecilia reticulata and sturgeon fry were used instead of the experimental host due to ease of handling and susceptibility. After 15 days post infection, guppy bathed in VNN-infected tissue culture with 104 TCID50 showed clinical signs similar to naturally infected Golden grey mullet, and the mortality rate reached up to 100% in 75 dpi. When target organs were examined by cell culture isolation, serology, and histopathology, all revealed the presence of virus in the Guppy. Suspected supernatant injected to sturgeon fry through intravitreous injection and widespread vacuolation were observed in brain and spinal cord buy IHC and Real time PCR were negative. In conclusion, with attntion to obtained results in this investigation such as ecological factors, clinical signs, histopathological, virological and bacteriological results, molecular analysis, (IHC, IFAT, PCR), TEM demonstration, serological and hematological findings, it could be confirmed that VNNV was the main causative agent for disease outbreak in Golden grey mullet in Southern coastline of Caspian Sea.

Description: The objective of this study was to investigate the effects of black carp introduction in chines carp poly culture as well as its effect on the growth, survival rate and production of chines carp species. This study conducted in two consecutive year. The period of culture in first year was during Jun 1 until Des 5 ,2009 and in second year it was from Jun 4 to Dec, 1,2010 .The experiment were started by selecting 9 earth end pond each with 800 m2 area and was performed in triplicate with 2 treatment and one control , each year .The ponds were treated by liming and fertilizer before filling water and then stocked with 3000 chines carp (140 Silvercarp, 42 Grass carp , 42 Common carp and 25 Bighead carp) while the number of Black carp in treatments 1, 2, 3 and 4, respectively, 250, 500, 75 and 125 pieces in Ha, blackcap was added to the control species respectively . The number of fish species harvested at end of culture in treatment 1, 2 and control .The total fish species harvested at the end of first year was 2276 out 1904 species released. The number of fishes catch in treatment 1,2 and control were 637,658and 573 species. the statistical analysis showed that average weight of black carp were 407±150 gr in treatment 1, 234±121 gr in treatment 2 with the minimum and maximum weight of 242 and 780 gr in first treatment as well as 110 and 750 gr in second treatment the production of black carp in first treatment were 42 kilogram per hectare and 60 kilogram in second treatment. The growth rate were higher in second treatment than first one. In second year the investigation repeated in triplicate with treatment3, 4 and one control. The total stocking density in treatment 3,4 and control were 665,633, and 574 pieces. Statistical analysis showed that total weight of black carp in third treatment was2049 ±614gr and in forth treatment was 2361 ±60 gr where the minimum and maximum values were 3115 gr and 1025 gr respectively. The survival rat of black carp in treatment 3 higher than in were treatment 4 while the growth in treatment 4 was higher treatment 3. The total production of black carp in treatment 3 and 4 were 123 and 181 kilogram per hectare respectively. The ANOVA test conducted for grass carp revealed that treatment 4 is significantly difference with treatment 3 and control. While for silver carp the treatment 4 is significantly difference with treatment 1 and control while for Big head carp the treatment 4 was significantly difference with control at 5% level (P〈%5). On second year the black carp reached to acceptable market size which could be compatible with other carp such as common carp and grass carp comparative analysis between different stocking density indicate that the stocking density with 125 pieces showed the highest positive effects.

Description: In this study, influence of modified atmosphere packaging on shelf life of trout (Oncorhynchus mykiss) (whole fish without visceral and without head and tail fish) stored in 4 to 6ºc was examined. Fish stored in MAP condition and control samples, in different time, were tested for spoilage chemical factors (TVN, PV and pH), microbial parameters (total viable count, clostridium botulinum) and sensory factors too. Mixed gases including co2 (30-50%), N2 (40-65%) and o2 (0 to 20%) were used for trout (without head and tail =6 treatments) and (whole fish without visceral and control = 2 treatments) statistical the analysis results showed that examined factors were significant difference during storage (P〈0.001). Mixed gases haven t had inhibitory effect on spoilage factors (chemical and microbial parameters). However spoilage process was delayed. Increasing of chemical and microbial changes in control samples was higher than treatment samples especially TVN. The results also showed that shelf life of control samples stored 4-6ºc were between 6-12 days but in MAP samples were 19 days. Mixed gases including CO2 (40%), N2 (55%) and O2 (5%) were the best formula and the shelf life of fish (without head and tail) was 16 days where it was 19 days in whole fish (Lack of visceral). The results showed that storage of trout in MAP condition facilities storage and increasing of fish shelf life too.

Description: Larvae of the chironomid midges (blood worms) are known as use full live food for cultured aquatics. There are endemic blood worms in sediments of the earthponds of Bafgh area at center of Iran. This study tended to identification of two chironomid species, Chironomus aprilinus Meigen, 1830 and Dicrotendipes sudanicus Freeman, 1959 & 1961, from Iran as a new record. Culture of blood worms was successful in glass thanks with 16h. Light, at 24-26°C temperature. Eggs were incubated after 14 days. Suitable concentration of harvesting is 1-2 egg masses (1250eggs/m2). Enrichment of earth ponds or plastic thanks including sedime is offered for culture of chironomid larvae. Collectors prepared with low cost materials are useful for collection of egg masses. May, June and July are the best time for egg collection. Large blood worms are mainly available in winter. Results of body analysis of the larvae indicated high dry matter with good quality.

Description: Bony fish stocks are being legally utilized by 134 fishing cooperatives in the Iranian side of the Caspian Sea mainly through beach seine. This fishing method has been invariantly used over the past. Fifty years with actually no alteration or technical modifications in the fishing nets despite the substantial changes in the quality & quantity of bony fish resources. Based on the data obtained from the stock assessment project for bony fish, the amount of premature or young fish (substandard size) caught during the past decade has been far greater than those of authorized fish size and even the figures for certain species suggest heavy over fishing to such an extent that in the case of frisii kutum, there has been a reduction by 50% in the overall kutum landings. Thus in order to modify the technical design of the fishing with a view to alleviate the catch of non-standard fish and whereby prevent over fishing by beach seine, it seems imperative therefore to launch a biometric study on bony fish stocks particularly the two intended fish species namely Rutilus frisii kutum and mullet which would involve identification of their standard weight and length using pertinent mathematical equations as well as determining proper mesh size of the code-end and other parts of the nets & consequently the H. R, d/a coefficients. The operations related to project were carried out in the "Inland Aquaculture Research Institute" net mating plant and beach seine site in Anzali during 2005-7 which aimed at designing and constructing standardized beach seines and comparing the fishing performance & yields with those of ordinary beach seine nets under equal conditions with a ratio of 3:1. The results concerning the quality and quantity of fish caught during a whole fishing cycle were recorded in the related data forms. The statistical analysis consisting the comparison of the quality of fishing performance in both the experimental and control beach seine nets was made using T. student and excel, spss 12 software packages. The results indicated that the reduction in the amount of non-standard fish caught in standardized beach seine net (the experimental net) was meaningfully different from that of the ordinary (control) net showing the superior fishing performance of the former. The catch level of non-standard R.frisii kutum decreased from 67.1% in the control beach seine net down to 7.1% in the standardized beach seine net and these figures for mullet were 29.98% and 1.1% respectively. Based on the results obtained and considering the apparent superiority of the experimental net in catching greater number of mature fish and less number of premature or non-standard sized fish, the use of this modified beach seine net can be proposed to Iranian fisheries organization as a viable alternative to the traditional beach seine nets.

Description: Study and research on the fishing gears in northern waters of Persian Gulf (Khuzestan Province Coastal Waters) was carried out from March 2009 to February 2010. Sampling was done for trawl in summer and autumn, for purse seine in autumn and for gillnet and trap in all seasons. The average of total catch was in trawl 167.9 1.06 kg. The rate of bycatch in ± 207.89 and trap 1.66 ±270.17, gillnet 275.86 ± 48.4, purse seine 1795.6 ± trawl net was 87 percent, purse seine net 30.49 percent, gill net 70 percent and trap 6.19 percent. In this study, the number of bycatch species in trawl net are 38 species, gill net 36 species, trap 10 species and purse seine net 4 species. The result of two way ANOVA test in trawl net between bycatch and time, and between bycatch and place showed non-significant differences (p〉0.05, f=0.3) (p〉0.05, f=0.9). The result of two way ANOVA test in gillnet between bycatch and time, showed significant (p〈0.05, f=14.7), and between bycatch and place showed non-significant (p〉0.05, f=1.8). The result of two way ANOVA test in trap between bycatch and time and between bycatch and place was non-significant (p〉0.05, f=0.5). According to the result, rate of bycatch of trawl net is higher than other gear, so control and manage of bycatch in trawl is most important.

Description: Certain diseases such as bacterial , viruses, parasirtic ones Protozoa parasites Could severe mortalities in farmed shrimp last decades. Two hundred and seventy Fenneropenaeus indicus with the weight of 10± 1.09 were selected, transferred to Iran Shrimp Center and distributed to the treatmentsand controls (3 groups). The objective of our study was to describe the signs, prevalence, and intensity of Hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Syndrom Virus (WSSV) shrimp as group A and non-infected non- exposure to WSSV shrimp as group C in triplicate. The results revealed that Hemocytosis associated with a decrease in hyalinocytes and large-granulocytes (less than 8%) and a considerable increase in semi-granulocytes in group A and B in contrast to group C that Hyalinocyte increased. This infection made the shrimp susceptible to WSSV disease. It is believed that Hemocytosis causes a condition which can result in a rapid mortality among susceptible species, F. indicus when exposure to WSSV is occurred.

Description: Seafood business is one of the important subjects in fishery activation. It is covered a wide range of fishery activation from the fishing to the table. The fishery production success is depending to the many factors. Without considering the market conditions and suitable trading the fishery activations are not commercial. Therefore considering to the different subjects is necessary. This project is one of the road map seafood processing plans. The aim of this project was to study of the world fishery production, world fish processing, marketing and fish production and treading in Iran by using current different information.

Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.