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  • Life and Medical Sciences
  • 1970-1974  (1,189)
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  • 1
    Electronic Resource
    Electronic Resource
    6,8-Dihydroxypurine: A novel growth factor for mammalian cells in vitro, isolated from a commercial peptone (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 81 (1973), S. 281-284 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A substance promoting the growth of mammalian cells in vitro has been isolated from Bacto-peptone (Difco Labs., Detroit) and identified as 6,8-dihydroxypurine. This compound, which is isomeric with xanthine(2,6-dihydroxypurine), enhances the growth of one Chinese hamster and some other cell lines; xanthine itself is inactive. The biological action of the compound is discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040810216
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  • 2
    Electronic Resource
    Electronic Resource
    A brief chronicle on Harry Eagle (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 76 (1970), S. 241-252 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040760304
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  • 3
    Electronic Resource
    Electronic Resource
    A Calcium-sensitive lanthanum inhibition of amoeboid movement (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 81 (1973), S. 365-369 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of lanthanum ions upon amoeboid movement and upon the redistribution of dense cytoplasmic inclusions following centrifugation were investigated in Amoeba discoides. Treatment with low lanthanum concentrations produced a characteristic inhibited condition in which locomotion ceased while saltatory movements continued. The resorting of stratified cytoplasm by local internal cytoplasmic flow was unaffected. This contrasted with p-hydroxymercuribenzoate treatment which inhibited both locomotion and saltatory movements. The inhibitory effects of lanthanum ions were antagonised by calcium ions but not by magnesium ions. It is reasoned that lanthanum competes for a peripheral binding site thereby disrupting a calcium-mediated regulatory mechanism of amoeboid movement.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040810309
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  • 4
    Electronic Resource
    Electronic Resource
    A cell line which is temperature-sensitive for cytokinesis (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 80 (1972), S. 253-259 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have isolated several cell lines which are temperature-sensitive for growth. One of these appears to be temperature-sensitive for cytokinesis. It was isolated from a Syrian hamster cell line by selecting cells which were not killed by 1 μg/ml cytosine arabinoside at 39° but which grew normally at 31°. It shows an increased proportion of binucleate cells when shifted to the non-permissive temperature and time-lapse photomicroscopy shows that a high proportion of attempted mitoses fail at 39°, apparently at the stage of cytokinesis. The cells which have failed to complete mitosis reattach to the plate and have two normal-size nuclei but otherwise behave normally.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040800212
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  • 5
    Electronic Resource
    Electronic Resource
    A comparative histological study of mammalian boneResearch supported by grants HD-03677 and HD-50021 from N.I.C.H.D., National Institutes of Health, Bethesda, Md. (1974)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 144 (1974), S. 421-437 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This report adds to the available information on comparative histology of mammalian bone and introduces a semi-quantitative approach to its study. Bone samples consisted of large fragments of rib tibia and/or femur from humans, laboratory rodents and from animals that died at the Bronx Zoological Gardens. A total of 44 bone specimens representing six mammalian orders were available. Decalcified paraffin-embedded 10 μm histological cross-sections were examined at × 320. Qualitative observations were supplemented with measurements of the frequency and size distributions of Haversian and non-Haversian canals. The relative distribution of lacunae was also ascertained. The standard textbook description of mammalian bone as consisting mainly of secondary osteons was not generally seen except in Primate (especially human) bone. Rats showed a few scattered osteon-like structures, but bones of Marsupialia, Insectivora, Artiodactyla and Carnivora were entirely devoid of them. Generally, vascular bone with longitudinal canals was seen except in Lorisidae which showed a reticular type of bone. The distribution of primary longitudinal canals and the number of “filled” or “apparently empty” lacunae/unit area of bone varied both inter-species and among different areas in the same bone. Large areas of acellular and non-vascular bone were encountered in all specimens. This preliminary study revealed that species differences i bone microstructure involve the relative distribution of the same basic components which lend themselves to quantitative treatment. Comparative investigations of bone histology at many ontogenetic and phylogenetic levels should yield significant quantitative information in bone biology.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051440404
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  • 6
    Electronic Resource
    Electronic Resource
    A comparative ultrastructural and behavioral study of the antennal sensory sensilla of the parasitoid Cardiochiles nigriceps (hymenoptera:Braconidae)Approved for publication as TA 10689 by the Director, Texas Agricultural Experiment Station in cooperation with the U.S.D.A. Supported in part by a grant-in-aid of research of the Society of the Sigma Xi and Cotton Incorporated CI-71-599. The authors wish to thank Dr. E. L. Thurston, coordinator of the Electron Microscopy Center at Texas A&M University for his advice and use of the facilities during this study. (1974)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 142 (1974), S. 329-349 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Scanning and transmission electron microscopy studies were conducted on the antennal sensory sensilla of the hymenopteran parasitoid, Cardiochiles nigriceps Viereck, of the family Braconidae. Distinct morphological differences were found between the chemoreceptors of the male and female. Curved, non-fluted, thin-walled sensilla were found to be very abundant on the male and restricted in location and number on the female. Trichoid, placoid and fluted basiconic sensilla were numerous on the antennal flagella of both sexes. Smooth basiconic sensilla were restricted in number to one per flagellar segment in both sexes. Behavioral data suggest that bent-tipped, thick-walled sensilla unique to the female are involved in detecting a chemical(s) emitted from the host, Heliothis virescens (Fab.).
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051420308
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  • 7
    Electronic Resource
    Electronic Resource
    A comparison between the dermal glands in two insects Rhodnius prolixus (Hemiptera) and Calpodes ethlius (Lepidoptera) (1972)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 136 (1972), S. 495-503 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The results of histochemical tests performed on the type “B” dermal gland in Rhodnius and the Verson's gland in Calpodes at different stages in their secretory cycle are presented. The secretions of both glands are slightly acidic sulphated mucosubstances. They differ in their main carbohydrate component which in Rhodnius is hyaluronic acid and in Calpodes is sialic acid. The glands, though structurally similar, differ in size, number, and development.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051360406
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  • 8
    Electronic Resource
    Electronic Resource
    A comparison of ligand-induced redistribution of surface immunoglobulins, alloantigens, and concanavalin A receptors on mouse lymphoid cellsSupported in part by National Cancer Institute grant CA 08748. (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 83 (1974), S. 441-447 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Redistribution of surface immunoglobulins (Ig), H-2b, Thy-1.2 and TL. 1,2,3 alloantigens, and concanavalin A (Con A) receptors on mouse thymus, lymph node and spleen cells into “caps” induced by bivalent antibodies or ligands was compared by immunofluorescence. Surface Ig was capped rapidly following attachment of anti-Ig antibody at 37°. Capping of alloantigens and Con A receptors occurred very slowly following attachment of alloantibody or Con A, but much more rapidly after addition of a secondary bivalent antibody. An inverse relationship between the number of surface component sites per cell and the extent of capping of that component was observed. Capping of alloantigens sparsely represented on the cell surface was not inhibited by high concentrations of alloantibody, in contrast to capping of alloantigens present in greater quantities. These results suggest that factors in addition to molecular cross-linking may be involved in ligand-induced redistribution of cell surface components.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040830315
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  • 9
    Electronic Resource
    Electronic Resource
    A comparison of the effect of cytotoxic agents on agar colony forming cells, spleen colony forming cells, and the erythrocytic repopulating ability of mouse bone marrow (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 82 (1973), S. 309-317 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Three assays for bone marrow progenitor cells have been used to determine the effect of single doses of two cytotoxic agents, cyclophosphamide and vinblastine. The assays employed were the agar colony forming and spleen colony forming assays and the crythroid repopulating ability.In normal mice, there was little difference between the response of the progenitor cells assayed by the three methods, following cyclophosphamide: and no detectable difference following vinblastine.Bone marrow from continuously irradiated mice and bone marrow regenerating seven days following transplantation was also studied: in both these situations the proliferation rate of the progenitor cells is increased. Cyclophosphamide was found to be only slightly proliferation dependent with each assay. However, vinblastine was strikingly proliferation dependent. In irradiated mice and also in regenerating marrow the agar colony forming cells were many times more sensitive to this agent than were the other progenitor cells.These results show that under some but not all circumstances the agar colony forming and spleen colony forming cells behave similarly in C57BL mice, but are not a single population of cells.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040820219
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  • 10
    Electronic Resource
    Electronic Resource
    A cytologic analysis of the bag cell control of egg laying in AplysiaThis research is supported by grants NB08109 and NB07711 from the National Institutes of Health. (1970)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 132 (1970), S. 461-485 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A fine structural analysis of the ovotestis in Aplysia was undertaken in order to analyze the site of action of the bag cell hormone. Five stages of oocyte development are described. Of particular interest is the fact that the yolk seems to be synthesized primarily by the granular endoplasmic reticulum. In addition, small muscle cells whose long, thin processes surround the follicle of the ovotestis have been pointed out. This paper suggests that bag cell extract has a direct action on these small muscle cells causing them to contract and thus expel oocytes from the ovotestis. The evidence for this suggestion is that (1) these muscle cells are the most obvious effector cells in the ovotestis, (2) there are no signs of neural innervation of these muscles, (3) the time course for the liberation of the oocytes is so short that any other method of oocyte release is unlikely, (4) there is no cytologic evidence for any other expulsion process except muscular contraction, and (5) the ripe oocytes are attached to other cells of the wall of the ovotestis only by very small, simple junctions, thus making them the most likely cells to be expelled by muscular contraction.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051320407
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