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  • Electron microscopy  (474)
  • Rat  (409)
  • Springer  (847)
  • 1985-1989  (369)
  • 1970-1974  (478)
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Year
  • 1
    ISSN: 1435-604X
    Keywords: Brain tumour ; Rat ; Detection ; Fluorescence ; Laser ; Haematoporphyrin derivative
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract Laser-induced fluorescence has been used for the identification of brain tumours in rats, which have been previously given tumour-seeking haematoporphyrin derivative. A pulsed nitrogen laser (λ=337 nm) was used in conjunction with an optical multichannel analyzer. For both inoculated RG-2 and TCVC rat-brain-tumour models, the blue autofluorescence was strongly reduced in the tumour compared with normal brain tissue, and at the same time the characteristic red-drug signal increased. The contrast between tumour and normal tissue was strongly enhanced by forming the ratio between the two signals. Implications for possible improvement of tumour delineation in brain tumour surgery are discussed.
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  • 2
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    Colloid & polymer science 263 (1985), S. 116-119 
    ISSN: 1435-1536
    Keywords: Electron microscopy ; staining ; morphology ; nylon-12 ; orientation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The morphology of drawn and annealed sheets of nylon-12 was investigated by transmission electron microscopy of stained sections, and the results compared with equivalent small-angle X-ray scattering (SAXS) patterns. A three-component structure was observed, consisting of crystalline (C) and amorphous (A) regions in the microfibrils and an interfibrillar component whose density was deduced to be intermediate between that of the C and A regions. The crystallite width was given satisfactorily by a Guinier analysis of the SAXS profile.
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  • 3
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    Cellular and molecular life sciences 41 (1985), S. 265-266 
    ISSN: 1420-9071
    Keywords: Rat ; hydrochlorothiazide ; pain threshold ; antinociceptive activity ; analgesic activity ; morphine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Hydrochlorothiazide, acutely injected in rats, has a weak analgesic activity per se and potentiates and prolongs the antinociceptive effect of morphine.
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  • 4
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    Calcified tissue international 10 (1972), S. 238-251 
    ISSN: 1432-0827
    Keywords: Growth ; Bone ; Rat ; Tetracycline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La vitesse de croissance normale en longueur de la métaphyse proximale du tibia est déterminée chez le rat Sprague-Dawley entre les âges de 20 et 100 jours, en utilisant la méthode à la tétracycline. Le taux de croissance ne varie que légèrement dans les groupes d'âges différents. Il est plus élevé chez les animaux jeunes et décroit considérablement en fonction de l'augmentation de l'âge. Les rats mâles présentent une croissance plus élevée que les femelles. Cette étude a pour but de mettre au point une méthode permettant de déterminer les facteurs expérimentaux, liés à la croissance en longueur du rat.
    Abstract: Zusammenfassung Die normale Längenwachstums-Geschwindigkeit der proximalen Wachstumsplatte der Tibia wurde bei Sprague-Dawley-Ratten in einem Alter zwischen 20 und 100 Tagen mittels der Tetracyclinmethode gemessen. Die Wachstumsgeschwindigkeit variierte nur wenig innerhalb der einzelnen Altersgruppen. Die Geschwindigkeit war bei jungen Tieren am höchsten und nahm mit zunehmendem Alter beträchtlich ab. Männliche Ratten wuchsen schneller als weibliche. Diese Arbeit dient als Grundlage, um die experimentelle Beeinflussung des Längenwachstums der Ratte abschätzen zu können.
    Notes: Abstract The rate of normal growth in length from the proximal growth plate of the tibia in the Sprague-Dawley rat was measured between 20 and 100 days of age using the tetracycline method. The growth rate varied only slightly within different age groups. The rate was highest in young animals and decreased considerably with increasing age. Male rats grew faster than female. This study is intended to provide a base for an evaluation of experimental influence on the growth in length of the rat.
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  • 5
    ISSN: 1432-0983
    Keywords: Ribosomal protein ; Immunological homology ; Yeast ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Polyclonal antibodies raised against ribosomal protein (r-protein) L2 of Schizosaccharomyces pombe were used to check for cross-reaktions with total r-proteins of rat liver. Using this procedure, the rat liver r-proteins, L4 and L24, were identified as being immunologically related to yeast L2. In addtional, homologies between rat liver L4 and L24 were detected. The possible implications for the regulation of r-protein synthesis are discussed.
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  • 6
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    Current genetics 14 (1988), S. 163-170 
    ISSN: 1432-0983
    Keywords: Plant mtDNA ; Electron microscopy ; Restriction enzymes ; Hairpin structures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Broad bean (Vicia faba) mitochondrial DNA (mtDNA) includes three circular plasmids: mt-plasmid 1 (1,704 ntp), mt-plasmid 2 (1,695 ntp) and mt-plasmid 3 (1,476 ntp). Partially replicated circular forms of these mt-plasmids have been observed in electron microscope preparations. Restriction enzymes that cleave either mt-plasmid 2 (but not mt-plasmids 1 and 3) or mt-plasmid 3 (but not mt-plasmids 1 and 2) were used to generate linear forms of partially replicated mt-plasmid 2 and mt-plasmid 3 molecules. Analyses of these linearized replicative intermediates, observed by electron microscopy, indicated that in both mt-plasmid 2 and mt-plasmid 3 replication originates at a specific location and proceeds in the same, single direction around the molecules. The replication origins of mt-plasmid 2 and mt-plasmid 3 map close to sequences that can fold into hairpin structures.
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  • 7
    ISSN: 1432-0983
    Keywords: Sugar beet ; Cytoplasmic male sterility ; Mitochondrial DNA ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNAs from normal (N) and male sterile (S) cytoplasms of sugar been have been isolated and investigated by electron microscopy. The results showed that mtDNA was composed of a heterogeneous population of circular molecules. Their contour lengths varied from 0.28 to 51 μm, but unlike in the case of maize, a large difference was not observed in the distribution of molecular classes greater than 1.0 μm between N and S cytoplasms of sugar beet. On the other hand, N and S cytoplasms were shown to contain their own characteristic combinations of small circular mtDNA species with lengths between 0.28 μm and 0.6 μm. Mitochondrial DNAs from various sources of male-sterile cytoplasms were analyzed by agarose gel electrophoresis to determine the extent of cytoplasmic variation. Additional low molecular weight DNA bands appeared in all male-sterile lines examined, and as a result, three distinctive banding patterns were recognized. These data are in general agreement with those based upon restriction endonuclease digestion of mt and chloroplast DNAs and the genetic analysis of fertility restoration in test crosses.
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  • 8
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    Journal of molecular evolution 26 (1987), S. 347-357 
    ISSN: 1432-1432
    Keywords: Ribosome structure ; Electron microscopy ; Image analysis ; Evolutionary lineages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Multivariate statistical analysis and classification techniques are powerful tools in sorting noisy electron micrographs of single particles according to their principal features, enabling one to form average images with an enhanced signal-to-noise ratio and a better reproducible resolution. We apply this methodology here to determining the characteristic views of the large (50S) ribosomal subunits from the eubacteriumEscherichia coli and the archaebacteriaMethanococcus vannielii, Sulfolobus solfataricus, andHalobacterium marismortui. Average images were obtained of the subunit in the common crown and kidney projections, but views of the particle in orientations intermediate between these two extremes were also elucidated for all species. These averages show reproducible detail of up to 2.0 nm resolution, thus enabling the visualization and interspecies comparison of many structural features as a first step toward comparing the actual three-dimensional structures. Our results disprove evolutionary lineages recently postulated on the basis of electron microscopical images of ribosomal subunits.
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  • 9
    ISSN: 1432-1432
    Keywords: Carcinoembryonic antigen ; Evolution ; Gene family ; Human ; Rat ; Synonymous substitutions ; Silent molecular clock ; Evolutionary trees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Various rodent and primate DNAs exhibit a stronger intra- than interspecies cross-hybridization with probes derived from the N-terminal domain exons of human and rat carcinoembryonic antigen (CEA)-like genes. Southern analyses also reveal that the human and rat CEA gene families are of similar complexity. We counted at least 10 different genes per human haploid genome. In the rat, approximately seven to nine different N-terminal domain exons that presumably represent different genes appear to be present. We were able to assign the corresponding genomic restriction endonuclease fragments to already isolated CEA gene family members of both human and rat. Highly similar subgroups, as found within the human CEA gene family, seem to be absent from the rat genome. Hybridization with an intron probe from the human nonspecific cross-reacting antigen (NCA) gene and analysis of DNA sequence data indicate the conservation of noncoding regions among CEA-like genes within primates, implicating that whole gene units may have been duplicated. With the help of a computer program and by calculating the rate of synonymous substitutions, evolutionary trees have been derived. From this, we propose that an independent parallel evolution, leading to different CEA gene families, must have taken place in, at least, the primate and rodent orders.
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  • 10
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    Journal of molecular evolution 24 (1987), S. 260-271 
    ISSN: 1432-1432
    Keywords: Mouse ; Rat ; Two-dimensional electrophoresis ; Quantitative variability of proteins ; Qualitative variability of proteins ; Protein classes ; Membrane proteins ; Organ-specific proteins ; Regulatory genes ; Speciation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Proteins of membranes and cytosols were extracted from the livers and brains of mice (inbred strain DBA/6J) and rats (inbred strain DA/Han) and separated by two-dimensional electrophoresis (2-DE). The 2-DE patterns were compared with regard to qualitative (spot position) and quantitative (spot intensity) characteristics of the proteins of these two species. The following results were obtained: (1) Brain had more (higher percentage) conservative proteins (proteins found in both mice and rats) than liver; (2) plasma membranes had more conservative proteins than the cytosols; (3) organ-unspecific proteins contained more conservative proteins than relatively organ-specific proteins; (4) the pattern of distribution of genetic variability among different classes of proteins represented by findings 1–3 was the same for the qualitative and quantative characteristics of the proteins; and (5) some observations indicated that quantitative variability occurred more frequently among proteins than did qualitative variability. Our conclusion is that regulatory sequences in the DNA (regulatory genes) are subjected to functional constraints that differ in strength among different classes of proteins by the same ratios as the constraints acting on the structural genes. The overall effect of the selective pressure is, however, less stringent for regulatory genes than for structural genes. The results obtained here by comparing two different species are very similar to previous results we obtained by studying different subspecies (inbred strains of the mouse). From this finding arises a new concept: the study of molecular evolution on the basis of different classes of proteins. Our results were compared with data from the literature that were obtained in part from studies on cultured cells. The comparison suggested that cultured cells have lost their tissue-specific proteins, and so generate predominantly extremely conservative proteins.
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  • 11
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    European journal of nutrition 26 (1987), S. 43-51 
    ISSN: 1436-6215
    Keywords: Dietary protein ; Vitamin A ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Es wurde der Einfluß von Qualität und Quantität von Nahrungsproteinen auf die Verteilung einer einzigen massiven Dosis von Vitamin A in verschiedenen Organen wachsender Wistar-Ratten untersucht. Die Untersuchungen wurden mit Casein- und Bengal-Gram-Diäten mit 20 % und 10 % Proteingehalt und auch mit radioaktiv markiertem Retinylacetat durchgeführt. Die Ergebnisse zeigen, daß die Leberspeicherung sowohl von Vitamin A aus der Nahrung als auch die einer einzigen starken Vitamin-A-Gabe (20 000 I.U.) stark herabgesetzt war bei Ratten, die nach der Bengal-Gram-Diät gefüttert wurden, verglichen mit Ratten, die auf Casein-Diät gesetzt waren. Im Gegensatz zur Leberspeicherung ist das Vitamin-A-Niveau im Plasma in allen Gruppen vergleichbar. Füttern niedriger Protein-Qualität reduzierte die Gewebeverteilung von [3H]-Retinylacetat sowohl bei Kontrollratten als auch bei solchen, denen eine massive Dosis Vitamin A gegeben wurde. Diese Untersuchung läßt vermuten, daß sowohl schlechte Qualität als auch unzureichende Mengen von Nahrungsproteinen nachteilige Einflüsse auf den Vitamin-A-Zustand wachsender Ratten haben.
    Notes: Summary The influence of the quality and the quantity of dietary proteins on the distribution of a single massive dose of vitamin A in various organs of growing Wistar strain rats has been studied by using casein and bengal gram diets at 20 % and 10 % protein levels. The distribution of [3H]-retinyl acetate in various tissues was also investigated in these dietary conditions. The results show that the hepatic storage of dietary as well as a single massive dose (20,000 I.U.) of vitamin A was profoundly decreased in the rats fed on bengal gram diets as compared to those fed on casein diets. Regardless of hepatic stores, the plasma vitamin A levels were comparable in all the groups. Feeding of low quality of protein reduced the tissue distribution of [3H]-retinyl acetate in control as well as rats given a massive dose of vitamin A. This study suggests that both the poor quality and the inadequate quantity of dietary protein are detrimental influences on the vitamin A status of the growing rats.
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  • 12
    ISSN: 1573-0832
    Keywords: Microsporon audouinii ; Pyrrolnitrin ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Scanning and transmission electron microscopy showed that a ceiling quantity (1.56 mcg) of antifungal antibiotic Pyrrolnitrin caused heavy damage to dermathophyteMicrosporon audouinii Gruby CBS 313-54in vitro. Suitable preparation technique made it clear that the changes involved consisted of hyphal collapse on the edge of the culture, with loss of euplasmic organelles identity and cell autolysis. The cell wall, however, was apparently undamaged. These findings fit in with the suggestion that the mode of action of the antibiotic leads to generalised lipoproteic membranes damage. They must, however, be considered as representing the result of the terminal phase of cell distress.
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  • 13
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    Development genes and evolution 194 (1985), S. 429-432 
    ISSN: 1432-041X
    Keywords: Tail bud ; Tail gut ; Gut ; Organogenesis ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The formation of the tail portion of the primitive gut was investigated by light and electron microscopy in 10- and 11-day rat embryos. The observations permit the conclusion that the tail gut does not form as a posterior extension of the hindgut but originates from the tail bud mesenchyme by mechanism analogous to the secondary neurulation. It includes cell condensation, aquisition of apicobasal polarity and the radial, rosette-like arrangement around a central cavity. These cells bear the cytological characteristics of both the absorptive epithelial cells and the mesenchymal cells at their apical (adluminal) and abluminal ends respectively.
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  • 14
    ISSN: 1420-9071
    Keywords: Rat ; alloxan diabetes ; intestinal villus ; alkaline phosphatase ; sucrase ; epithelial cells ; villus-crypt axis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sucrase activity in enterocytes isolated from the villus crypt axis was found to increase in all regions of the villus from day 2 after induction of diabetes, and the increase continued until day 4. In contrast, alkaline phosphatase activity increased mainly in the apical one-third of the villus-crypt column, and the increase occurred abruptly on day 4 with increase in food intake.
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  • 15
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    Cellular and molecular life sciences 42 (1986), S. 169-171 
    ISSN: 1420-9071
    Keywords: Rat ; olfactory pathway selective lesions ; plasma corticosterone ; lactacidemia ; adrenal glands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In comparison to control rats, basal plasma corticosterone level and lactacidemia significantly increased in rats submitted to a bilateral lesion of the lateral olfactory tract and/or the anterior branch of the anterior commissure. Only the anterior branch of the anterior commissure induced hyperglycemia; that of the lateral olfactory tract exerted an opposite effect.
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  • 16
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    Cellular and molecular life sciences 41 (1985), S. 273-275 
    ISSN: 1420-9071
    Keywords: Rat ; embryo ; embryo ; rat ; β-hydroxybutyrate ; diabetes ; maternal ; fetal malformation ; malformation ; fetal ; ketone bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to investigate further the relationship between maternal diabetes and fetal malformation, rat embryos were grown in vitro in the presence of β-hydroxybutyrate, one of the ketone bodies produced by diabetics. At 10 mM, β-hydroxybutyrate produced minor abnormalities and at 20 mM it produced major abnormalities in rat embryos.
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  • 17
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    Calcified tissue international 14 (1974), S. 283-289 
    ISSN: 1432-0827
    Keywords: Sodium fluoride ; Osteoporosis ; Bone ; Strength ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'utilisation thérapeutique de fluorure de sodium a été recommandée dans le traitement de diverses maladies osseuses ostéopéniques. Cet emploi est basé principalement sur les effects ostéosclérotiques bien connus du fluorure de sodium, mais on connait mal son effet sur la force de résistance osseuse. L'influence de diverses concentrations de fluorure de sodium sur la force de résistance osseuse de jeunes rats soumis à des régimes riches et pauvres en calcium a été étudiée. L'administration de fluorure de sodium augmente le diamètre osseux, indiquant une stimulation de la formation de l'os périosté, mais la force de résistance osseuse est réduite ou non modifiée par l'ingestion de fluor.
    Abstract: Zusammenfassung Die therapeutische Verwendung von Natriumfluorid ist für eine Anzahl von Knochenmangel-Krankheiten empfohlen worden. Die Empfehlungen basieren hauptsächlich auf den bekannten osteosklerotischen Wirkungen von Natriumfluorid; über dessen Effekt auf die Knochenstärke ist wenig bekannt. In dieser Arbeit wurde der Einfluß verschiedener Konzentrationen von Natriumfluorid auf die Knochenstärken von wachsenden Ratten mit hoher und niederer Calciumeinnahme untersucht. Die Verabreichung von Natriumfluorid erhöhte den Knochendurchmesser, was auf eine Stimulierung der Periostbildung hinwies, die Knochenstärke wurde jedoch durch Fluorideinnahme herabgesetzt oder nicht beeinflußt.
    Notes: Abstract The therapeutic use of sodium fluoride has been recommended in a variety of osteopenic bone diseases. The recommendations are based mainly on the known osteosclerotic effects of sodium fluoride and little information is available as to its effect on bone strength. The influence of various concentrations of sodium fluoride on bone strength in growing rats on high and low calcium diets was studied. The administration of sodium fluoride increased bone diameter, indicating stimulation of periosteal bone formation, but bone strength was reduced or not affected by fluoride ingestion.
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  • 18
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    Calcified tissue international 13 (1973), S. 113-129 
    ISSN: 1432-0827
    Keywords: Kinetics ; Growth ; Epiphyses ; Hypophysectomy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La largeur de la métaphyse tibiale, la zone indifférenciée, la zone sériée et les cellules en dégénerescence ont été observées chez des rats Sprague-Dawley normaux et hypophysectomisés. La production cellulaire de la métaphyse est déterminée sur la base de la croissance osseuse longitudinale déterminée par l'oxytétracycline et la taille des cellules en dégénérescence. La diminution de la croissance osseuse longitudinale, en fonction de l'augmentation de l'âge et après hypophysectomie, est due partiellement, à la diminution de production cellulaire et partiellement à une décroissance de la taille des cellules en dégénérescence dans la métaphyse. L'influence de la production cellulaire et de l'activité mitotique prédomine.
    Abstract: Zusammenfassung Die Breite der proximalen Wachstumsplatte der Tibia, deren undifferenzierter und säulenförmiger Zone und die Größe der nahe bei der Metaphyse auftretenden degenerativen Zellen wurden in normalen und hypophysektomierten Sprague-Dawley-Ratten bestimmt. Die Zellproduktion in der Wachstumsplatte wurde aus dem longitudinalen Knochenwachstum berechnet, welches mittels Oxytetracyclin und der Größe der degenerativen Zellen bestimmt wurde. Es wurde festgestellt, daß die Abnahme des longitudinalen Knochenwachstums bei zunehmendem Alter und nach Hypophysektomie zum Teil einem Rückgang in der Zellproduktion, zum Teil einer Verminderung der Größe der degenerativen Zellen in der Wachstumsplatte zuzuschreiben ist. Der Einfluß der Zellproduktion, und somit der mitotischen Aktivität, herrscht vor.
    Notes: Abstract The width of the proximal growth plate of the tibia, its undifferentiated and columnar zone and the size of the degenerative cell close to the metaphysis, were determined in normal and hypophysectomized Sprague-Dawley rats. The cell production in the growth plate was calculated from the longitudinal bone growth determined with oxytetracycline and the degenerative cell size. It was found that the decrease in longitudinal bone growth with increasing age and after hypophysectomy, is due partly to a decrease in cell production, and partly to a decrease in degenerative cell size in the growth plate. The influence of cell production and thus the mitotic activity predominates.
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  • 19
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    Calcified tissue international 16 (1974), S. 93-107 
    ISSN: 1432-0827
    Keywords: Dentinogenesis ; Globules ; Pyrophosphatase ; Calcification ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Three-day-old rats were fixed by perfusion with glutaraldehyde and thin slices were cut of the first molar germs. The slices were treated with EDTA and “activated” with buffered solutions containing Mg2+, Ca2+ or Zn2+. Incubation was carried out in buffered solutions (pH 8.5) containing inorganic pyrophosphate and Pb2+. In the Mg2+-activated specimens incubation products were localized to the plasma membranes in the stratum intermedium and the subodontoblastic area. Lead deposits were found on the periphery of the dentinal globules. Incubation products were more randomly distributed in Ca2+-activated specimens whereas those activated with Zn2+ displayed a deposition of lead precipitates mainly corresponding to that seen after activation with Mg2+. The findings are discussed in reference to the localization of alkaline phosphatase in the dentin-producing tissues and it is proposed that the results are indicative of the presence of an inorganic pyrophosphatase in these tissues.
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  • 20
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    Calcified tissue international 8 (1971), S. 165-171 
    ISSN: 1432-0827
    Keywords: Bone ; Ceramic ; Tetracycline ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Un implant céramique non poreux est testé au niveau du fémur de rat en ce qui concerne son adhésivité à l'os. Un certain nombre de techniques morphologiques sont utilisées pour examiner le rapport entre l'implant et l'os néoformé. La microscopie électronique par transmission et la microscopie par fluorescence après marquage à la tétracycline ont donné les meilleurs résultats. Un rapport étroit entre l'os minéralisé et la céramique a été noté en microscopie électronique. Par marquage à la tétracycline, il semble que l'implant puisse stimuler la formation osseuse.
    Abstract: Zusammenfassung Ein unporöses keramisches Implantat in Rattenfemora wurde auf seine Fähigkeit geprüft, sich mit Knochen zu binden. Eine Anzahl morphologischer Techniken wurde verwendet, um die Beziehung zwischen den Oberflächen von Implantat und neuem Knochen zu untersuchen. Transmissions-Elektronenmikroskopie und Fluoreszenzmikroskopie nach Tetracyclinmarkierung waren die erfolgreichsten Techniken. Eine enge Beziehung zwischen mineralisiertem Knochen und dem Keramikimplantat konnte mit der Transmissions-Elektronenmikroskopie nachgewiesen werden. Das Aussehen der Tetracyclinmarkierung im keramischen Implantat deutet darauf hin, daß dieses wahrscheinlich die Fähighkeit hat, Knochenbildung zu erhöhen.
    Notes: Abstract A nonporous ceramic implant in rat femora was evaluated as to its ability to bond to bone. A number of morphologic techniques were utilized to examine the interfacial relationship of the implant to new bone. Transmission electron microscopy and fluorescence microscopy after tetracycline labelling were the most successful techniques. An intimate relationship between mineralized bone and the ceramic was demonstrated by transmission electron microscopy. The appearance of tetracycline labelling at the ceramic interface indicates that the implant may have capacity to enhance bone formation.
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  • 21
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    Calcified tissue international 8 (1971), S. 287-303 
    ISSN: 1432-0827
    Keywords: Calcification ; Bone ; Matrix ; Apatite ; Nucleation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Du collagène d'os compact de mouton est préparé par décalcification dans I'EDTA et à partir de tendons de queux de rats, par extraction dans l'acide acétique et reconstitution dans NaCl. Le dépôt d'apatite dans le collagène osseux de mouton dans une solution de calcification métastable est étudié chimiquement et par microscopie électronique. Le collagène osseux est un bon catalyseur de nucléation pour le dépôt minéral, alors que le collagène de tendons de rat ne l'est pas. Le dépôt minéral du collagène osseux se produit en deux phases cinétiques séparées, une phase rapide de nucléation et une croissance cristalline, donnant naissance à de petits ilots calcifiés et une seconde phase lente de croissance dans des régions ne comportant pas de zones catalytiques. La seconde phase de dépôt minéral paraît être le résultat d'une diffusion inhibée d'ions à travers les fibrilles collagènes alignées, laissant de larges régions de collagène sans minéral, bien que le tampon reste hautement sursaturé. La microscopie électronique permet de penser que les zones de catalyse pourraient avoir un rapport avec la périodicité de 640 Å de collagène, mais l'importance d'un matériel noncollagènique, lié au collagène, n'est pas à exclure. L'activité catalytique faible du collagène reconstitué n'est pas liée à la présence d'inhibiteurs faiblement liés, bien que des inhibiteurs puissent être intimement liés à ce type de collagène, qui pourrait être absent du collagène osseux. La différence d'activité catalytique pourrait intervenir dans la calcification physiologique. Une hypothèse plus générale pour la nucléation de la phase minérale dans les systémes biologiques est nécessaire.
    Abstract: Zusammenfassung Kollagen wurde aus kompaktem Schafsknochen mittels EDTA-Entkalkung und aus Rattenschwanzsehnen durch Essigsäureextraktion und Rekonstitution mit NaCl gewonnen. Die Apatitablagerung aus einer metastabilen Verkalkungslösung auf Schafsknochenkollagen wurde chemisch und im Elektronenmikroskop untersucht. Es zeigte sich, daß das Knochenkollagen ein guter Nukleationskatalysator für die Mineralablagerung ist, was beim Rattenschwanzkollagen nicht zutraf. Im Knochenkollagen erfolgte die Mineralablagerung in zwei getrennten kinetischen Phasen: einer raschen Phase der Nukleation und des Kristallwachstums, welche kleine verkalkte Inseln entstehen läßt, und einer zweiten langsamen Phase, welcher das Wachstum in Bezierken, die keine katalytischaktiven Stellen einschließen, zuzuschreiben ist. Diese zweite Phase der Mineralablagerung wird als Resultat einer verminderten Ionendiffusion durch die enganeinanderliegenden Kollagenfibrillen angesehen, wodurch weite Kollagenbereiche ohne Mineral bleiben, obwohl der Puffer stark übersättigt ist. Elektronenmikrographien ließen vermuten, daß die katalytischaktiven Stellen in einem gewissen Verhältnis zur 640 Å-Periodizität des Kollagens stehen; es konnte jedoch nicht ausgeschlossen werden, daß nicht-kollagenhaltiges Material, welches an Kollagen gebunden ist, ebenfalls eine Rolle spielt. Die schlechte katalytische Aktivität des rekonstituierten Kollagens konnte nicht auf die Anwesenheit von schwachgebundenen Hemmstoffen zurückgeführt werden, obwohl Inhibitoren stark an dieses Kollagen gebunden sein könnten, die jedoch im Knochenkollagen nicht vorhanden sind. Die Unterschiede in der katalytischen Aktivität können mit der physiologischen Verkalkung in Beziehung stehen. Eine allgemeinere Hypothese für die Nukleation einer Mineralphase in biologischen Systemen wäre erforderlich.
    Notes: Abstract Collagen was prepared from compact sheep bone by decalcification with EDTA and from rat tail tendons by acetic acid extraction and reconstitution with NaCl. The deposition of apatite in sheep bone collagen in a metastable calcification solution was studied chemically and by electron microscopy. The bone collagen was shown to be a good nucleation catalyst for mineral deposition, while rat tail collagen was a poor catalyst. Mineral deposition in bone collagen occured in two separate kinetic phases, a rapid phase of nucleation and crystal growth, giving rise to small calcified islands, and a second slow phase, ascribed to growth in regions not involving the catalytic sites. This second phase of mineral deposition is considered to be the result of impaired ion diffusion through the closely-aligned collagen fibrils, thus leaving large areas of the collagen free of mineral even though the buffer remains highly supersaturated. Electron micrographs suggested that the catalytic sites might be in some relationship to the 640 Å periodicity of collagen, but a role for non-collagenous material bound to the collagen has not been excluded. The poor catalytic activity of reconstituted collagen was not due to the presence of loosely-bound inhibitors, although inhibitors could be strongly bound to this type of collagen and be absent from bone collagen. The differences in catalytic activity may have a bearing on physiological calcification. A more general hypothesis for nucleation of a mineral phase in biological systems is required.
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  • 22
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    Calcified tissue international 15 (1974), S. 213-220 
    ISSN: 1432-0827
    Keywords: Acid phosphatase ; Electron microscopy ; Shell Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Acid phosphatase activity was mainly localized in the lysosomes in all the regions of the outer epithelium. The transitional portion of the outer epithelium showed more intense activity than the other regions. During shell regeneration the activity of this portion decreased to a minimum level at 12 hours and was restored to normal at 72 hours. The other regions showed no change of activity during shell regeneration. It is postulated that the acid phosphatase in the transitional protion is responsible for conferring calcifiability to the organic matrix of the shell.
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  • 23
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    International journal of biometeorology 32 (1988), S. 17-20 
    ISSN: 1432-1254
    Keywords: Epilepsy ; Electromagnetic fields ; Rat ; Audiogenic seizure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract In order to study the possible association between epileptic seizures and natural electromagnetic fields, 32 female audiogenic seizure (AGS)-susceptible rats were exposed to simulated 10 kHz and 28 kHz atmospherics and to a sinusoidally oscillating magnetic field with a frequency of 100 Hz and field strength of 1 A/m. After the electromagnetic exposure, seizures were induced in the rats with a sound stimulus. The severity of the seizure was determined on an ordinal scale, the audiogenic response score (ARS). The time from the beginning of the sound stimulus to the onset of the seizure (seizure latency) and the duration of the convulsion was measured. No differences from the control experiments were found in the experiments with simulated atmospherics, but the 100 Hz magnetic field increased the seizure latency by about 13% (P〈0.02). The results do not support the hypothesis that natural atmospheric electromagnetic signals could affect the onset of epileptic seizures, but they suggest that AGS-susceptible rats may be a useful model for studying the biological effects of electromagnetic fields.
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  • 24
    ISSN: 1432-1254
    Keywords: Atmospherics ; Carrageenan inflammation ; Rat ; Susceptibility ; Correlations
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    Topics: Geography , Physics
    Notes: Abstract Between the mean daily density of 28 kHz atmospherics and the onset of epileptic fits there is a highly significant correlation coefficient (r) of 0.30; there is a negative coefficient of −0.20 between the fits and the mean daily density of 10 kHz atmospherics. The onset of heart infarction is correlated with 28 kHz atmospherics (r=0.15). Furthermore, we have discovered that sudden deafness is also correlated with certain configurations of atmospherics. In this paper we report the following correlation coefficients between the inflammatory reaction of rats to a carrageenan injection (rci) into a hind paw and the mean daily pulse rate of atmospherics of the same day:r=0.49 for the 8 kHz atmospherics (P〈0.02) andr=0.44 for the 10 kHz atmospherics (P〈0.04). The correlations between rci reaction and other atmospherics (12 and 28 kHz) are smaller and not significant. By the method of multiple linear regression we found a multipleR=0.54 between rci reaction and the 8 and 10 kHz atmospherics (the regression function for the rci reaction is 0.15+0.004×8 kHz+0.002×10 kHz,P〈0.05).
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  • 25
    ISSN: 1432-0983
    Keywords: Electron microscopy ; Replicative intermediates
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    Topics: Biology
    Notes: Summary Some physicochemical properties of the mitochondrial DNAs (mtDNA) from plants of flax, broad bean and mung bean, and from tissue culture cells of jimson weed, soybean, petunia and tobacco were determined. Circular molecules were observed in electron microscope preparations of each mtDNA. In soybean, petunia, broad bean and mung bean mtDNAs, the circular molecules had a continuous distribution of lengths (ranges between 1 to 36 kb, and 1 to 126 kb), heavily skewed toward smaller molecules. Eighty-six percent of the flax circular molecules were from 27 to 54 kb in size, and 78% of the jimson weed circular molecules were from 4 to 15 kb. Replicative forms of 1.2–1.6 kb circular molecules were observed in electron microscope preparations of broad bean mtDNA.
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  • 26
    ISSN: 1432-1351
    Keywords: Rat ; Melatonin ; Circadian rhythm ; 5-hydroxytryptophan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rhythm in melatonin production in the rat is driven by a circadian rhythm in the pineal N-acetyltransferase (NAT) activity. Rats adapted to an artificial lighting regime of 12 h of light and 12 h of darkness per day were exposed to an 8-h advance of the light-dark regime accomplished by the shortening of one dark period; the effect of melatonin, triazolam and fluoxetine, together with 5-hydroxytryptophan, on the reentrainment of the NAT rhythm was studied. In control rats, the NAT rhythm was abolished during the first 3 cycles following the advance shift. It reappeared during the 4th cycle; however, the phase relationship between the evening rise in activity and the morning decline was still compressed. Melatonin accelerated the NAT rhythm reentrainment. In rats treated chronically with melatonin at the new dark onset, the rhythm had already reappeared during the 3rd cycle, in the middle of the advanced night, and during the 4th cycle, the phase relationship between the evening onset and the morning decline of the NAT activity was the same as before the advance shift. In rats treated chronically with melatonin at the old dark onset or in those treated with melatonin 8 h, 5 h and 2 h after the new dark onset during the 1st, 2nd and 3rd cycle, respectively, following the advance shift, the NAT rhythm reappeared during the 3rd cycle as well but in the last third of the advanced night only. Neither triazolam nor fluoxetine together with 5-hydroxytryptophan administered around the new dark onset facilitated NAT rhythm reentrainment after the 8-h advance of the light-dark cycle.
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  • 27
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    Archives of microbiology 142 (1985), S. 333-339 
    ISSN: 1432-072X
    Keywords: Photosynthesis ; Membrane structure ; Electron microscopy ; Photosynthetic bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The organization of photosynthetic membranes in the cytoplasm of the photosynthetic bacterium Rh. viridis has been examined by several techniques for electron microscopy. Thin sections of membrane stacks show that the regular lattice of membrane subunits reported in other studies can be observed in thin section. Tilting of sections in the electron microscope shows that the regular lattices of several membranes overlap in a way that suggests they are in register with each other. This observation can be confirmed by freeze-fracture images in which a regular arrangement of membrane lattices can be observed, each perfectly aligned. Analysis of the spacings of membrane pairs shows that the photosynthetic membranes of Rh. viridis are very closely apposed. The mean diameter of two membranes is 160A, and the average space between two such membranes is only 42A. When a recently developed atomic level model of Rh. viridis reaction center is superimposed against these spacings, each reaction center extends from the surface of its respective membrane far enough to make contact with an apposing membrane. The limited free space between membranes and regular alignment of lattices has a number of implications for how this membrane is organized to carry out the process of energy transfer.
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    Archives of microbiology 146 (1986), S. 267-274 
    ISSN: 1432-072X
    Keywords: Photosynthesis ; Membrane structure ; Electron microscopy ; Ectothiorhodospira ; Serial thin sectioning ; Three dimensional reconstruction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The three dimensional organization of the complete photosynthetic apparatus of the extremely halophilic, bacteriochlorophyll b containing Ectothiorhodospira halochloris has been elaborated by several techniques of electron microscopy. Essentially all thylakoidal sacs are disc shaped and connected to the cytoplasmic membrane by small membraneous “bridges”. In sum, the lumina of all thylakoids (intrathylakoidal space) form one common periplasmic space. Thin sections confirm a paracrystalline arrangement of the photosynthetic complexes in situ. The ontogenic development of the photosynthetic apparatus is discussed based on a structural model derived from serial thin sections.
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  • 29
    ISSN: 1432-072X
    Keywords: Acidaminococcus fermentans ; Glutamate fermentation ; Electron microscopy ; Immunocytochemistry ; Post-embedding labelling ; Antibody-gold complexes ; Protein A-gold complexes
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    Topics: Biology
    Notes: Abstract We have investigated the in situ location of glutaconyl-CoA decarboxylase and 2-htdroxyglutaryl-CoA dehydratase in Acidaminococcus fermentans using the antibody-gold and protein A-gold techniques carried out as a post-embedding immunoelectron microscopic procedure. Polyclonal antisera were raised in rabbits against homogeneous fractions of the enzymes. Anaerobically grown cells of A. fermentans of the late exponential growth phase were fixed with 0.2% glutaraldehyde and 0.3% formaldehyde (final concentrations) in the growth medium. Dehydration of the cells was achieved with methanol. The cells were embedded in the low temperature embedding resin Lowicryl K4M. The markers indicative for antigenic sites of the two enzymes unequivocally demonstrate that the sodium pump glutaconyl-CoA decarboxylase is located at the cell periphery being a membrane-bound enzyme as expected whereas 2-hydroxyglutaryl-CoA dehydratase is a soluble cytoplasmic enzyme.
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  • 30
    ISSN: 1432-072X
    Keywords: Bacteriolysis ; Penicillin ; Autolysis ; Cell wall ; Electron microscopy ; Staphylococcus aureus
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    Topics: Biology
    Notes: Abstract The actual reason for the penicillin-induced bacteriolysis of staphylococci was shown to be the “punching” of one or a few minute holes into the peripheral cell wall at predictable sites. These perforations were the result of the lytic activity of novel, extraplasmatic vesicular structures, located exclusively within the bacterial wall material, which we have named “murosomes”. In untreated staphylococci the punching of holes into the peripheral wall is a normal process which follows cross wall completion and represents the first visible step of cell separation. Under penicillin, however, analogous holes are punched by the murosomes at sites of presumptive cell separation even if no sufficient cross wall material had been assembled before at this site (but had rather been deposited at other sites). Consequently, because of the internal pressure of the protoplast, lytic death is the inevitable result of this perforation of the protective peripheral wall. Hence, the real mechanism of penicillin-induced bacteriolysis in staphylococci is considered to be mainly the result of a special morphogenetic wall defect: bacteriolysis is taking place regularly when a cell separation process is no longer preceeded by sufficient cross wall assembly at the correct place. However, hypotheses which are based purely on some variations of overall biochemical processes like total wall enzyme activities or total wall synthesis are not regarded to be sufficient to explain this type of lytic death.
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    Archives of microbiology 142 (1985), S. 259-261 
    ISSN: 1432-072X
    Keywords: Methanogenic bacteria ; Plasmid isolation ; Alkaline lysis ; CsCl gradient ; Restriction endonuclease mapping ; Electron microscopy ; DNA homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Of 21 recently isolated strains of methanococci, one was found to harbor a small, cryptic, low copy number plasmid. Reproducible recovery was achieved by alkaline lysis of cells pretreated with proteinase K in an osmotically stabilizing buffer. The plasmid was found to contain a singleAval site. No homology was detected between the plasmid and DNA from any of the other new strains or from five known species of methanococci.
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  • 32
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    Archives of microbiology 140 (1985), S. 338-342 
    ISSN: 1432-072X
    Keywords: Sporosarcina halophila ; Endospores ; Electron microscopy ; Heat resistance ; Ethanol resistance ; Germination ; Dipicolinic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporosarcina halophila forms endospores. Electron micrographs revealed ultrastructural similarity to spores of S. ureae. Spore germination indicated by loss of refractility, darkening, swelling and formation of new vegetative cells was followed by phase contrast light microscopy. To induce spore germination, the endospores needed to be heat avtivated. After activation, they were inoculated into nutrient broth medium supplemented with sea-water. Double concentrated sea-water was found to be optimal for germination. Similar to other bacterial endospores, the spores were found to be resistant to heat and ethanol. An ultraviolet absorbing substance was isolated from suspensions of free spores; it was identified to be pyridine-2,6-dicarboxylic acid (DPA) usually present in bacterial spores. DPA was detected in amounts ranging from 5–7% of the spore dry weight; it was not detected in extracts of vegetative cells.
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    Archives of microbiology 141 (1985), S. 85-90 
    ISSN: 1432-072X
    Keywords: C. sporosphaeroides ; Citrate lyase ; Regulation ; Purification ; Properties ; Electron microscopy
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    Topics: Biology
    Notes: Abstract Cells of Clostridium sporosphaeroides which were grown on citrate contained citrate lyase and citrate lyase acetylating enzyme, but no detectable citrate synthase and citrate lyase deacetylase activities. Citrate lyase from C. sporosphaeroides was purified to homogeneity as judged by polyacrylamide gel electrophoresis and high performance liquid chromatography. In contrast to the enzyme from Clostridium sphenoides, the addition of l-glutamate was not necessary for activity and stabilization of the enzyme. The purified enzyme had a specific activity of 34 U/mg protein and was comparable to other citrate lyases with respect to its molecular weight and subunit composition. Electron microscopic investigations showed that similar to the lyase from C. sphenoides and in contrast to all other citrate lyases examined so far, the majority of the enzyme molecules was present in “star” form.
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    Archives of microbiology 143 (1986), S. 400-402 
    ISSN: 1432-072X
    Keywords: E. coli relA +/relA ; Starvation survival ; Guanosine tetraphosphate ; Electron microscopy ; Glycogen
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    Topics: Biology
    Notes: Abstract Amino acid-starved cells of Escherichia coli relA +, which contain a large number of glycogen particles, are able to survive in phosphate buffer for a longer time period than their relaxed counterparts. With regard to NH 4 + starvation differences in the survival of both strains were not found. NH 4 + starved cells of E. coli relA are able to synthesize glycogen but amino acid-starved cells of the relA strain are not. We suggest that the synthesis of glycogen triggered by guanosine tetraphosphate during amino acid starvation is responsible for the prolonged viability of the E. coli relA + strain.
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  • 35
    ISSN: 1432-072X
    Keywords: Bradyrhizobium ; Electron microscopy ; Mutants ; Nitrogen fixation ; Nodulation ; Soybean ; Symbiosis ; Transposon Tn5
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    Topics: Biology
    Notes: Abstract The genome of the slow-growing Bradyrhizobium japonicum (strain 110) was mutagenized with transposon Tn5. A total of 1623 kanamycin/streptomycin resistant derivatives were screened in soybean infection tests for nodulation (Nod) and symbiotic nitrogen fixation (Fix). In this report we describe 14 strains possessing a stable, reproducible Nod+Fix- phenotype. These strains were also grown under microaerobic culture conditions to test them for free-living nitrogen fixation activity (Nif). In addition to strains having reduced Fix and Nif activities, there were also strains that had reduced symbiotic Fix activity but were Nif+ ex planta. Analysis of the genomic structure revealed that the majority of the strains had a single Tn5 insertion without any further apparent physical alteration. A few strains had additional insertions (by Tn5 or IS50), or a deletion, or had cointegrated part of the vector used for Tn5 mutagenesis. One of the insertions was found in a known nif gene (nifD) whereas all other mutations seem to affect different, hitherto unknown genes or operons. Several mutant strains had an altered nodulation phenotype, inducing numerous, small, widely distributed nodules. Light and electron microscopy revealed that most of these mutants were defective in different stages of bacteroid development and/or bacteroid persistence. The protein patterns of the mutants were inspected by two-dimensional gel electrophoresis after labelling microaerobic cultures with l-(35S)methionine. Of particular interest were mutants lacking a group of proteins the synthesis of which was known to be under oxygen control. Such strains can be regarded as potential regulatory mutants.
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  • 36
    ISSN: 1432-072X
    Keywords: Aggregation substance ; Enterococcus faecalis ; Electron microscopy ; Field emission scanning electron microscopy ; Immunogold labelling technique ; Sex pheromone system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electron microscopy and high resolution scanning electron microscopy we investigated the appearance and distribution of the aggregation substance. The “hairs” increase in number with increasing exposure to sex pheromones (maximum density: 1300/μm2). We show that these structures are unequally distributed over the cell surface, even if the cells were induced by sex pheromones for a long period of time. Statistical analysis of the unequal distribution indicates that aggregation substance is incorporated into pre-existing “old” cell-walls and that this incorporation shows a saturation ca. 40 min after addition of sex pheromones.
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  • 37
    ISSN: 1432-072X
    Keywords: Thiobacillus ferrooxidans ; Sulfur production ; Sulfur oxidation ; Inhibitors ; Uncouplers ; Electron microscopy
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    Topics: Biology
    Notes: Abstract The intermediary production of elemental sulfur during the microbial oxidation of reduced sulfur compounds has frequently been reported. Thiobacillus ferrooxidans, an acidophilic chemolithoautotroph, was found to produce an insoluble sulfur compound, primarily elemental sulfur, during the oxidation of thiosulfate, trithionate, tetrathionate and sulfide. This was confirmed by light and electron microscopy. Sulfur was produced from sulfide by an oxidative step, while the production from tetrathionate was initiated by a hydrolytic step, probably followed by a series of chemical reactions. The oxidation of intermediary sulfur was severely inhibited by sulfhydryl-binding reagents such as N-ethylmaleimide, by the addition of uncouplers or after freezing and thawing of the cells, which probably damaged the cell membrane. The mechanisms behind these inhibitions have not yet been clarified. Finally, it was observed that elemental sulfur oxidation by whole cells depended on the medium composition. The absence of sulfate or selenate reduced the sulfur oxidation rate.
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  • 38
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA of plants ; Electron microscopy ; Suspension culture ; Vicia faba
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    Topics: Biology
    Notes: Summary A comparative analysis of the Vicia faba mitochondrial genome in whole plants and in longterm suspension culture has been conducted. Restriction fragment patterns of the mtDNA isolated from these two sources were notably different. Electronmicroscopic analysis also revealed significant differences. Large circular mtDNA patterns shifted from a 37–80 kb subpopulation, which was predominant in whole plants, to 18–34 kb subpopulations although in both classes notable quantities of circular molecules of 80 to 120 kb and more were also found. Both in whole plant and suspension culture cells very large circular DNAs were observed. Some of them had lengths nearly 290 kb and could be considered as evidence of the existence of master chromosomes. The minicircular DNA population was also altered. In the suspension culture we observed a notable increase of percentage of minicircles with sizes near 1 kb. Simultaneously, the percentage of minicircles with sizes near 3.5–10 kb significantly increased in suspension culture cells. In addition, a new peak (10–12 kb) of minicircles appeared. Copy number alterations for some sequences homologous to CCC1A, CCC1B and CCC2 (Negruk et al. 1982, 1985) were shown. Southern hybridization revealed the existence of a family of minicircles having sizes 1.4–2 kb with predominance of CCC1A, CCC1B and CCC2. The copy numbers of CCC1B and some minor minicircles was changed in the suspension culture when compared with the whole plants.
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  • 39
    ISSN: 1432-2242
    Keywords: Plant mitochondrial genome ; Minicircle DNA ; Electron microscopy ; Beta vulgaris L.
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    Topics: Biology
    Notes: Summary The structure of mitochondrial DNA (mt-DNA) from sugarbeet (Beta vulgaris L.) has been studied by biochemical methods and electron microscopy. It was found to be complex multipartite consisting of two main classes of molecules: high molecules weight (HMW) mtDNA and low molecular weight (LMW) mtDNA. The HMW mtDNA consists of rosette-like structures and globules resembling chromomeres (150–200nm). A typical rosette has a protein core and radially stemming closed DNA loops (from 0.6-1.5 μm). The number of loops in a rosette varies from 16–30. The bulk of HMW mtDNAs are represented by interconnected rosettes (total contour length about 130–160 μm, 403–496 kbp). Such large circular DNAs may be evidence of the master chromosome arrangement of the sugarbeet genome. Globules and rosettes are interconnected by thick and thin DNA fibrils, along which nucleosome- and nucleomere-like structures are distributed. The LWM mtDNA is composed of two groups of supercoiled circular molecules, 0,2–1.5 μm and 0.02–0.05 μm in size. Electrophoretic analysis demonstrated that LWM mtDNA is represented by minicircle plasmid-like DNA molecules of 1.3, 1.4 and 1.6 kbp.
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  • 40
    ISSN: 1432-2242
    Keywords: Electron microscopy ; Genetic variation ; Mitochondrial DNA ; Oryza sativa L. ; Plasmid-like DNA
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    Topics: Biology
    Notes: Summary Mitochondrial (mt) plasmid-like DNA was found in most of more than 100 rice cultivars (Oryza sativa L.) by the use of 0.7% agarose gel electrophoresis (AGE). The DNA varied in molecular weight and number. By electron microscopy, small circular DNAs of different sizes could be detected in addition to the DNAs of high molecular weight, even in cultivars in which mt plasmid-like DNA was not detected by AGE. The detection of the mt plasmid-like DNAs by AGE did not depend on their presence or absence, but on their high stoichiometry. The relationship between cytoplasms with mt plasmid-like DNAs and varietal (for example, Indica rice) groups was close. The geographical distribution of cytoplasms is discussed.
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    Cell & tissue research 110 (1970), S. 97-107 
    ISSN: 1432-0878
    Keywords: Muscle Spindles ; Different types of sensory endings ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In einer Muskelspindel der Ratte, die an einer Serie alternierender Semidünn- und Ultradünnquerschnitte untersucht wurde, wurden zwei benachbart an „nuclear chain“-Fasern gelegene Nervenendformationen unterschiedlicher Bauweise festgestellt. Die beiden Endformationen sind mit ein und derselben Nervenfaser der Gruppe II verbunden und daher als Bestandteile einer sekundären sensorischen Endigung zu betrachten. Die Nervenendformation an einer der beiden „nuclear chain“-Fasern hat anulo-spirale Form. Die Nervenendformation an der anderen „nuclear chain“-Faser weist am Querschnittsbild eine Anzahl von Axonen auf, die zwischen Plasmalemm und Basalmembran der intrafusalen Muskelfaser eng aneinanderliegen. Nicht alle Axonquerschnitte stehen in direktem Kontakt mit der intrafusalen Faser. Das terminale Axon scheint sieh nach Eintritt unter die Basalmembran der intrafusalen Faser mehrfach in relativ dünne Äste unregelmäßigen Verlaufs zu teilen. Diese Form der Endigung könnte ein Korrelat der sog. „flower spray“-Endigung im Sinne Ruffinis (1898) darstellen. Die beiden morphologisch unterschiedlichen Endformationen innerhalb der sekundären Endigung gleichen einander und den Endformationen der primären Endigung bezüglich folgender Ultrastrukturmerkmale: 1. Es besteht synaptischer Kontakt zwischen Axon und intrafusaler Muskelfaser (synaptischer Spalt durchschnittlich 200 Å) ohne Zwischenlagerung von Basalmembranmaterial; 2. die terminalen Axonabschnitte liegen direkt unter der Basalmembran der intrafusalen Muskelfaser und sind nicht von Schwannschen Zellen bedeckt; 3. Mangel an synaptischen Bläschen; 4. desmosomenartige Verhaftungen zwischen Zellmembranen von Axon und intrafusaler Faser; 5. dyadenartige Anlagerungen des sarkoplasmatischen Retikulums an die Zellmembran der intrafusalen Faser im Bereich des synaptischen Spaltes. Nach unseren derzeitigen Vorstellungen sprechen diese Ultrastrukturmerkmale für eine rezeptorische Natur der beschriebenen Nervenendigungen.
    Notes: Summary In a rat muscle spindle transversally cut into a series of alternating semithin and ultrathin sections, two different forms of nerve terminations were found on two neighbouring nuclear chain fibres. The two nerve terminations were connected to the same group II nerve fibre and are consequently constituents of one particular secondary sensory ending. The nerve termination on one of the two nuclear chain fibres is of the anulo-spiral type. The nerve termination on the second nuclear chain fibre shows a number of axons lying closely together between plasma membrane and basement membrane of the intrafusal muscle fibre. Not all of these axons are in direct contact with the intrafusal fibre. The terminating nerve fibre seems to be divided into several branches of rather small diameters and irregular courses. It is suggested that this kind of termination could be a correlate of the so-called “flower spray” type of sensory endings in muscle spindles. The two morphologically different nerve terminations in the secondary ending have the following ultrastructural characteristics in common with those of the primary ending: 1) Synaptic contact between axon and intrafusal muscle fibre (synaptic gap about 200 Å) without interposition of basement membrane material; 2) terminal axons located beneath the basement membrane layer of intrafusal muscle fibres without covering by Schwann cells; 3) lack of synaptic vesicles; 4) desmosome-like structures between plasma membranes of axon and intrafusal muscle fibre, and 5) dyads of the sarcoplasmic reticulum adjacent to the synaptic cleft. According to present knowledge these features indicate that all of these endings are sensory ones.
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  • 42
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    Cell & tissue research 111 (1970), S. 316-345 
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Histophysiology of median eminence ; Avian neurohypophysis ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les effets de l'adénohypophysectomie et de diverses sollicitations de l'axe hypothalamo-hypophysio-corticosurrénalien sur l'ultrastructure de l'Eminence Médiane (E.M.) ont été étudiés chez le Pigeon. 1. Chez le Pigeon entier, l'Eminence Médiane Caudale (E.M.C.) se distingue de l'Eminence Médiane Rostrale (E.M.R.) essentiellement par l'absence dans les deux couches les plus externes (couches palissadique et superficielle) de l'E.M.C. de granules de gros calibres (1600 à 1900 Å), la rareté de granules de diamètre moyen (1200–1400 Å) et la prédominance de petites vésicules à cœur dense de 600–800 Å. 2. La préhypophysectomie entraine: a) dans l'E.M.R. la quasi disparition de granulations dans les deux couches externes; b) dans l'E.M.C. la ≪vidange≫ de nombreux axones, mais un enrichissement relatif, parmi les granulations restantes, des granulations de gros calibre (1600–1900 Å) aux dépens des granules de plus petit calibre. 3. Un shock insulinique entraine des modifications du même ordre: a) déplétion des granules denses, limitée dans ce cas à la portion la plus antérieure des deux couches externes de l'E.M.R.; b) enrichissement relatif des granulations de moyen (1200–1400 Å) et de gros (1600–1900 Å) calibre dans l'E.M.C. avec, en plus dans l'E.M.C., un enrichissement en vésicules de type synaptique. 4. Un traitement à la métopirone produit un accroissement du nombre des granulations de moyen (1200–1400 Å) calibre dans les couches externes de l'E.M.R. et de l'E.M.C., et un enrichissement important de l'E.M.C. en vésicules de type synaptique. 5. Le traitement à la prednisolone conduit à un enrichissement très marqué des couches externes de l'E.M.R. en grains de 1200–1400 Å, et à un enrichissement des couches externes de l'E.M.C. en granulations de 1000 Å. Ces résultats sont discutés dans la perspective des régulations hypothalamo-corticotropes, particulièrement en ce qui concerne les granules de 1200–1400 Å.
    Notes: Summary The effects of adenohypophysectomy, and of several experimental interventions on the hypothalamo-pituitary-adrenal cortical axis have been studied in relation to the fine structure of the median eminence in the pigeon. 1. In control animals, the following morphological features of the caudal median eminence (C.M.E.) distinguish it from the rostral median eminence (R.M.E.): a) the absence in both external layers of the C.M.E. of large (1,600–1,900 Å) electron-dense granules, b) the presence in the C.M.E. of a small number of medium-size (1,200–1,400 Å) granules, and c) the predominance in the C.M.E. of small (600–800 Å) dense-core vesicles. 2. Adenohypophysectomy leads to: a) almost complete disappearance of electron-dense granules in both external layers of the R.M.E., and b) “emptying” of numerous axons and a relative increase in the number of large (1,600–1,900 Å) granules in the C.M.E. 3. Insulin shock produces modifications similar to those of adenohypophysectomy. The depletion of electron-dense granules from the axons is, however, restricted to the most anterior part of the R.M.E., and, in the C.M.E., the relative increase in the number of larger granules affects the 1,200–1,400 Å and the 1,600–1,900 Å size granules. 4. Metopirone enhances the number of medium-size (1,200–1,400 Å) granules in the external layers of both the R.M.E. and the C.M.E. and causes a significant increase in the number of synaptic-like vesicles in the C.M.E. 5. Prednisolone treatment leads to a marked enrichment of the external layers of the R.M.E. with 1,200–1,400 Å granules, and of the external layers of the C.M.E. with 1,000 Å granules. These results have been discussed with special reference to the hypothalamic control of the adrenocorticotropic function, especially reviewing the role of the 1,200–1,400 Å granules.
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  • 43
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    Cell & tissue research 114 (1971), S. 61-72 
    ISSN: 1432-0878
    Keywords: Corpora cardiaca ; Neurosecretion ; Insects ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les corpora cardiaca de l'adulte de Locusta migratoria sont formés de deux régions bien individualisées ce qui nous a permis de reconnaître la sécrétion propre des différents types de neurosécrétion. Dans la région nerveuse, nous distinguons par la taille des grains trois types de neurosécrétion dense classique et un quatrième type d'aspect clair. Dans la région »propre« non nerveuse, les cellules ont des caractères nettement endocriniens et sont mélangées à un seul type d'axones neurosécréteurs.
    Notes: Summary The corpora cardiaca of adult Locusta migratoria consist of two well separated areas, a fact which permits the differentiation between intrinsic and extrinsic neurosecretory material. In the neural area three types of electron dense “classical” neurosecretory granules, and a fourth more lucent type can be distinguished according to size. In the non-neural “glandular” area typical endocrine cells mingle with only one type of neurosecretory axons.
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  • 44
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    Cell & tissue research 115 (1971), S. 565-578 
    ISSN: 1432-0878
    Keywords: Testis ; Reptiles ; Sertoli cells ; Glycogen ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les cellules de Sertoli du testicule de Lacerta vivipara ont été étudiées en microscopie électronique chez des animaux récoltés entre le printemps et l'automne pendant deux années et chez des animaux hypophysectomisés en automne. Ces cellules contiennent de nombreuses mitochondries de petite taille à crêtes lamellaires, des ribosomes libres, un reticulum endoplasmique lisse moyennement développé, plusieurs petits dictyosomes formant l'appareil de Golgi, des liposomes et des microtubules. Elles renferment aussi de nombreux corps denses de grande taille qui paraissent être de nature lysosomiale. Le glycogène a été particulièrement étudié. Il est formé de particules β dispersées au hasard dans le hyaloplasme. Des variations saisonnières dans la teneur en glycogène ont été notées. Chez les hypophysectomisés, les cellules de Sertoli contiennent de grandes quantités de ce métabolite dont les particules sont concentrées dans des petites plages, souvent autour des liposomes. Les rôles possibles des cellules de Sertoli sont discutés: soutien et apport de nourriture aux cellules germinales, production d'hormones et phagocytose des corps résiduels. Les variations de la teneur en glycogène sont également discutées.
    Notes: Summary Sertoli cells of the testis of Lacerta vivipara have been studied electron microscopically in animals obtained between spring and autumn during two years and in animals hypophysectomized in autumn. These cells contain numerous small mitochondria with lamellar cristae, free ribosomes, smooth endoplasmic reticulum moderately developed, several small dictyosomes forming the Golgi complex, lipid droplets and microtubules. There are numerous dense bodies of large size with an heterogeneous content which seem to be of lysosomial nature. Glycogen consists of β particles dispersed at random in the hyaloplasm. Seasonal variations in the content of glycogen are noted. In hypophysectomized animals Sertoli cells contain large amounts of that metabolite whose particles are concentrated in small areas often around the lipid droplets. Possible role of the Sertoli cells concerning mechanical support and nutrition of the germinal cells, production of hormones and phagocytosis of residual bodies are discussed. The variations in the glycogen content are also discussed.
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  • 45
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    Cell & tissue research 116 (1971), S. 151-156 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Innervation ; Receptors ; Rat ; Pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the adrenal cortex of the rat and the pig is investigated with the electron microscope. Nerve fibers containing synaptic and two types of dense-cored vesicles come into contact with endocrine cells. There are no specialized pre- and postsynaptic membranes. The synaptic cleft is about 200 Å wide. Generally the basement membrane between nerve and cell is absent. These observations are discussed on the base of more recent experimental findings. Small fibers having an average diameter of about 0.2 to 0.5 μ and containing only tubules and filaments are considered to represent parts of an afferent nervous system.
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  • 46
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    Cell & tissue research 118 (1971), S. 97-112 
    ISSN: 1432-0878
    Keywords: Stratum corneum ; Man ; Non-fixed ; Non-dehydrated ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This is an electron microscopic study of non-fixed and non-dehydrated normal human stratum corneum from the lumbar region. Non-stained sections have a low contrast. In sections examined 3 days after skin biopsy the cytoplasm of the cells shows a uniform contrast or exhibits dark and light areas. A single layer delimits the cytoplasm from the intercellular space. The latter is partly filled out with substance. In sections stained 2 to 4 days after skin biopsy the fibrils are distinct. On the basis of the variations in their opacity and ultrastructure three types of horny cells are clearly distinguishable. In cells of type 1 intensely stained keratohyalin and less opaque fibrillar substance occur. A distinct keratin pattern is not found. In cells of type 2 the fibrils show areas with distinct kerytohyalin and keratin pattern and transitional phases between these two stages of fibrillar differentiation. The keratin pattern representing the final stage of the fibrillar differentiation process is visualized through a successive “discoloration” of the filaments, whereas the interfilamentous substance retains the opacity of the keratohyalin. In cells of type 3 the entire fibrillar substance exhibits a keratin pattern. This consists of less opaque filaments with a diameter of 74 Å. The septa representing the interfilamentous substance are estimated as 30 Å at their thinnest points. These observations of the fibrils are completely comparable to the findings in fixed and dehydrated normal human stratum corneum. In sections stained particularly more than 18 days after skin biopsy the fibrils exhibit pronounced changes in their staining properties with concomitant decrease in distinctness or a complete extinction of the keratin pattern. The observations of the modified plasma membrane and the intercellular space in stained sections correspond to the findings in fixed and dehydrated normal human stratum corneum. The modified plasma membrane and the structures in the intercellular space appear with equal distinctness, whether the sections are stained 2 to 4, 6 to 12 or 14 to 21 days after skin biopsy.
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  • 47
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    Cell & tissue research 118 (1971), S. 467-481 
    ISSN: 1432-0878
    Keywords: Neutrophilic promyelocyte ; Human bone marrow ; Primary granulogenesis ; Phase contrast microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The developmental changes in the neutrophilic promyelocytes from normal human bone marrow have been analyzed by means of phase contrast and electron microscopy. This developmental phase is characterized by the elaboration of primary (azurophillysosomal) granules and the entire intracellular machinery is directed principally toward this goal. The promyelocyte stage has been subdivided into three arbitrary stages based upon morphological, histochemical and functional characteristics which relate to the onset, active production and cessation of primary granulogenesis.
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  • 48
    ISSN: 1432-0878
    Keywords: Adrenergic neurons ; “Short” and “long” systems ; Pre- and postnatal development ; Peripheral organs ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic innervation in the submaxillary gland, heart, kidney, small intestine, and accessory male genital organs and the development of the adrenal chromaffin cells and the sympathetic ganglia were studied in the rat from 15 days post coitum to 16 days post partum using the fluorescence histochemical method of Falck and Hillarp. The postnatal development of the noradrenaline concentrations in the heart and vas deferens was followed by fluorometric determinations. At about 15 days post coitum, the anlagen of the sympathetic chains were well visible in the form of two dorsal segmented columns of small branching sympathicoblasts exhibiting an intense catecholamine fluorescence. In the midline, ventrally to these two anlagen, another column of sympathicoblasts developed; this seemed to give rise to the prevertebral ganglia and to the short adrenergic neurons supplying the internal genital organs. At the level of the adrenal anlagen, small intensely fluorescent chromaffin cells were collected in two bilateral groups which became enclosed by adreno-cortical cells. This enclosure was, however, not complete even at two weeks post partum. Bundles of growing sympathetic nerves were visible in the periphery of the various organs studied at 19–21 days post coitum. A terminal innervation of the organs suggestive of a functional transmitter mechanism did not start to establish until at or immediately after birth. The final pattern of innervation was usually reached at about one week post partum, and the following development proceeded largely in the form of a quantitative increase in the number of nerves participating in the innervation apparatus. The adult level of noradrenaline in the heart and vas deferens was reached three to five weeks after birth. The small intestine was an exception in that the final pattern of innervation in the wall was attained immediately after birth. There was no overt difference in the rate of development of the terminal sympathetic innervation in organs supplied by short adrenergic neurons (accessory male genital organs) compared to the innervation of the submaxillary gland, heart and kidney, which receive classical long adrenergic neurons.
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  • 49
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    Cell & tissue research 119 (1971), S. 120-146 
    ISSN: 1432-0878
    Keywords: Red muscle ; Fibre types ; Small mammals ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Fasern des roten und langsamen M. soleus von Ratte, Kaninchen und Katze und des roten, jedoch schnellen, M. vocalis des Kaninchens wurden licht- und elektronenmikroskopisch untersucht und mit den verschiedenen Fasertypen aus dem M. tibialis anterior der Ratte und dem M. gastrocnemius des Kaninchens und der Katze verglichen. M. soleus und M. vocalis (einschließlich M. thyreoarytenoideus) enthalten nur einen mitochondrienreichen Fasertyp. Im schnellen M. vocalis ist der Z-Streifen schmal (50–60 nm), das sarcoplasmatische Reticulum ist gut entwickelt. Die Anordnung von Reticulum und Mitochondrion ist ähnlich wie in Herzmuskelzellen. Wie auch in anderen langsamen Muskeln verschiedener Tiere ist im M. soleus der Z-Streifen breit (100–120 nm), Triaden und Reticulum sind selten, und die Filamente bilden unregelmäßige Areale anstelle von Fibrillen. Hierin gleichen die Fasern des M. soleus den (mitochondrienreichen) C-Fasern eines entsprechenden gemischten Muskels; dagegen zeigen die Zwischentyp-(B-)Fasern schmale Z-Linien (50–70 nm), isodiametrische Fibrillen und mehr Triaden als die C-Fasern. Entgegen der bisherigen Vermutung, die auf der histochemischen Zuordnung der SoleusFasern zum Typ B und der Vocalis-Fasern zum Typ C beruht, ist daher anzunehmen, daß die langsamen motorischen Einheiten eines gemischten Muskels aus C- und nicht aus B-Fasern bestehen. In einigen Muskeln sind die Sarcomere der C-Fasern länger als die der B-(und A-) Fasern. Im M. tibialis anterior der Ratte verschwindet der Unterschied von 8,5% bei 2,6 μm Sarcomerlänge bei der Dehnung auf 2,8 μm mittlere Sarcomerlänge; vermutlich weil die Ruhedehnungskurve zunehmend steiler wird. Die isometrische Extraspannung im Tetanus ist bei 120% der Ruhelänge, d.h. bei 2,7 μm Sarcomerlänge. am größten. Daher muß bei 2,6 μm mittlerer Sarcomerlänge die Kraft der C-Fasern die der B-Fasern übertreffen. Rote Muskeln sind besser vaskularisiert als weiße Muskeln. Für die Mm. soleus und gastrocnemius der Katze verhalten sich die Kapillardichten (Kapillaren/mm2 Muskelfaserquerschnitt) wie 2,7∶:1. Dieser Wert entspricht dem Verhältnis zwischen den Größen für die Durchblutung (ml/min × 100 g) in Ruhe und bei maximaler Gefäßerweiterung.
    Notes: Summary Muscle fibres of the red and slow contracting soleus of rat, rabbit and cat and of the red however fast contracting thyreoarytenoid of rabbit are compared with different fibre types in the anterior tibial muscle of rat and in the gastrocnemius of rabbit and cat. With respect to fibre types soleus and thyreoarytenoid (including m. vocalis) are homogeneous and both being rich in mitochondria. The fast thyreoarytenoid shows a narrow Z-line (50–60 nm) and a well developed sarcoplasmic reticulum. The pattern of reticulum and mitochondria resembles more that of heart muscle cells than of skeletal muscle fibres. Like many slow contracting muscles of different animals the soleus fibres display a wide Z-line (100–120 nm), few triads, little reticulum and irregularly shaped areas of myofilaments instead of fibrils. In that soleus fibres equal fibres of type C (rich in mitochondria) in a corresponding heterogeneous muscle, whereas intermediate (type B) fibres reveal narrow Z-lines (50–70 nm), isodiametrically shaped myofibrils and more triads than C-fibres. Therefore it is far more likely that the slow motor units of a mixed muscle consist of C-fibres than of B-fibres. This is at variance with the histochemical designation of soleus fibres as type B and thyreoarytenoid fibres as type C. In some muscles in C-fibres the sarcomeres are longer than in B-(and A-)fibres. In the anterior tibial muscle of rat this difference is 8.5% at a mean sarcomere length of 2.6 μm, and disappears at a mean length of 2.8 μm, probably due to the steeper slope of the length tension diagram at rest. Since the isometric extratension in a tetanus is highest at 120% resting length (corresponding to about 2.7 μm sarcomere length), the force of C-fibres exceeds that of B-fibres at 2.6 μm but not at 2.8 μm sarcomere length. Red and white muscle differ with respect to vascularisation. The relation between the densities of capillaries in soleus and gastrocnemius of cat is 2.7∶:1 and equals the relation between the blood flows through these muscles during rest and maximum vasodilatation.
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    Cell & tissue research 119 (1971), S. 58-67 
    ISSN: 1432-0878
    Keywords: Ovary ; Rat ; Fowl ; Granulosa cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The granulosa cells of the ovarian follicle of the rat and the domestic fowl have been studied with the light and electron microscope. The nuclei of the granulosa cells were irregular with indentations and large in proportion to the cytoplasm of the cell. The mitochondria had a dense, dark matrix with only few cristac. The Golgi apparatus was moderately developed, located towards the oocyte in a juxtanuclear position. The endoplasmic reticulum was rather sparse. Lipid droplets were only occasionally encountered. Microtubules were regularly observed. The functions of the granulosa cells are discussed. Compared with the steroid-producing cells of the theca interna of the same follicles, the granulosa cells primarily are the nursing cells for the growing oocyte and mainly have the characteristics of protein forming cells.
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    Cell & tissue research 122 (1971), S. 283-300 
    ISSN: 1432-0878
    Keywords: Paraventricular nucleus ; Rat ; Synthesis of hormones ; Acid phosphatase ; Osmium impregnation ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural features of the paraventricular neurones of the non-treated rat are presented comparing them with those of the supraoptic neurones. No striking differences are seen between the general electron microscopic characteristics of the paraventricular and supraoptic neurones. The importance of adequate fixation to obtain good preservation of the neurones is emphasized, since inadequate fixation can cause e.g. artefactual appearance of “dark neurones”. The previously presented classification of the neurosecretory neurones into two categories (e.g. “light and dark neurones”) on the basis of the number of ribosomes is not considered justifiable, since their number can vary to a very great extent even within a single cell. The synthesis of neurosecretory products in the paraventricular neurones obviously follows the general mode of the synthesis of secretory proteins: ribosomes—RER—Golgi complex—secretory vesicles. On the basis of the localization of heavy metal deposits after osmium impregnation and demonstration of acid phosphatase the Golgi complex of the paraventricular neurones is found to be polarized. The direction of the polarity is discussed. The substructures of the dense cores of the neurosecretory granules and of the contents of the lysosomal dense bodies are nearly identical. Therefore it is considered impossible to determine positively the nature of the dark condensed material within the Golgi complex. The characteristics of the immature neurosecretory granules and the possibility of releasing neurosecretory products into the cytoplasm already within the perikarya are speculated.
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  • 52
    ISSN: 1432-0878
    Keywords: Smooth muscle ; Two-filament system ; Small intestine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of glycerinated smooth muscle from small intestine of adult rat was investigated by electron microscopy. In the central parts of the tissue blocks a two-filament system was found, consisting of parallel thick and thin filaments with regularly spaced interconnections, closely resembling that of striated muscle. In the peripheral parts of the blocks only thin filaments were found. The thin filaments were identified as actin by the formation of arrowhead complexes after incubation with heavy meromyosin.
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  • 53
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    Cell & tissue research 123 (1972), S. 47-54 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Exocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Elektronenmikroskopische Untersuchungen an der Neurohypophyse von Ratte und Forelle ergeben, daß sich eine Exocytose von Elementargranula an den Endigungen der neurosekretorischen Fasern nur selten abspielt. Es wird daher angenommen, daß die Abgabe von Hormonen in der Neurohypophyse in der Regel nach dem Muster des „membrane-release“ abläuft. 2. Die Exocytose wird nicht durch eine unmittelbare tangentiale Fusion der Membran des Elementargranulums mit dem Plasmalemm der Nervenendigung (Axolemm) eingeleitet. Vor allem bei Anwendung eines Goniometertisches wird erkennbar, daß vor der Exocytose zwischen Axolemm und Membran des Granulums eine Verbindung in Gestalt eines Stieles entsteht. Die Länge dieses Verbindungsstückes entspricht etwa 2 Axolemmdicken. An der Basis des Stiels im Axolemm tritt das Stoma auf, durch das der Inhalt des Granulums bzw. dieses selbst das Axonende verläßt. 3. Die Herkunft kleiner membrannaher Vesikel (Durchmesser 500 Å) in den Endigungen neurosekretorischer Nervenfasern in der Neurohypophyse konnte nicht geklärt werden. Anzeichen einer kompensatorischen Endocytose im Sinne von Nagasawa, Douglas und Schulz (1970) wurden nicht beobachtet.
    Notes: Summary 1. Electron microscopical investigations of the neurohypophysis in rat and trout reveal that exocytosis of neurosecretory elementary granules from the nerve endings occurs only rarely. The authors are of the opinion that hormone release in the neural lobe follows mainly the “membrane-release” pattern. 2. Exocytosis is not performed by tangential fusion of the elementary granule membrane and the plasmalemma of the nerve ending (axolemma). Administering the goniometer technique one can observe the appearance of a stalk-like structure connecting the two membranes. The basis of the stalk in the axolemma corresponds to the site of the stoma through which the core of the vesicle leaves the nerve ending. 3. The mechanism of the origin of small clear vesicles (diameter 500 Å approx.) near the axolemma of the neurosecretory terminal has not been elucidated. The authors did not observe equivalents of a compensatory endocytosis in the vicinity of granules released by exocytosis.
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  • 54
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    Cell & tissue research 121 (1971), S. 153-170 
    ISSN: 1432-0878
    Keywords: Neutrophilic myelocyte ; Human bone marrow ; Secondary granulogenesis ; Phase contrast microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The developmental changes in the neutrophilic myelocyte from normal human bone marrow have been analyzed by means of phase contrast and electron microscopy. This developmental stage is characterized principally by the elaboration of secondary (specific) granules. In addition, there is a modest decrease in cell size, a decrease in the number and mean size of primary (azurophil) granules, a decrease in the number of polysomes, free ribosomes and mitochondria, a depletion of rough endoplasmic reticulum, an increase in cytoplasmic glycogen, an increase in chromatin aggregations and a loss of nucleoli, and the formation of a markedly indented nucleus. The myelocyte stage has been subdivided into three arbitrary phases based upon morphological and functional characteristics which relate to the onset, active production and cessation of secondary granulogenesis.
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  • 55
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    Cell & tissue research 121 (1971), S. 531-547 
    ISSN: 1432-0878
    Keywords: Helical polyribosomes ; Skeletal muscle ; Polysomes ; Levator ani ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization and configurations of ribosomes in mature white skeletal muscle fibers of the rat were investigated. Differential visualization of ribosomes and glycogen granules was obtained by fixation with glutaraldehyde only and staining of the sections with uranyl acetate. Ribosomes are then electron dense and glycogen granules electron transparent. Their identity was ascertained by selective extractions of ribonucleic acid and polysaccharide. The vast majority of the ribosomes is not membrane-bound. They are located intermyofibrillarly (predominantly at the level of the I-bands), beneath the sarcolemma, and in the paranuclear cones of sarcoplasm. Occasionally short stretches of granular reticulum occur, often as characteristic double walled vesicles with ribosomes on the inner membrane only. Three main types of polysomal configurations are observed: rosettes of 4 to 6 ribosomes, helical arrays, and whorls of up to about 25 probably membrane-bound ribosomes. The average number of ribosomes in the extended helical configurations is estimated to be about 60. It is argued that these helices represent the polysomes instrumental in the synthesis of the large subunits of myosin. It is emphasized that helical polyribosomes are by no means typical of striated muscle, but probably represent a common configuration of large free polysomes.
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  • 56
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    Cell & tissue research 122 (1971), S. 1-14 
    ISSN: 1432-0878
    Keywords: Amnion ; Human amniotic plaques ; Fetal membranes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of amniotic and amniotic-plaque epithelia has been studied from normal term pregnancies. The columnar/cuboidal amniotic epithelial cells usually have apical or central nuclei, some free ribosomes, patches of granular endoplasmic reticulum, juxtanuclear Golgi complexes, rod-shaped mitochondria, lipid droplets and some glycogen granules. They have short, blunt microvilli which frequently branch and bathe in the amniotic fluid. The lateral plasma membranes enclose tortuous intercellular spaces which are always interrupted by variously folded processes and desmosomes. The epithelial cells rest on a basal lamina and exhibit highly folded basal processes. The amniotic epithelial cells are neither distinctly Golgi and fibrillar types nor “light” and “dark” in appearance. Amnion from near the umbilical cord contains many microscopic and several large plaques. Similar structures are not found on the reflected amnion. The microscopic plaques are whitish and translucent, whereas the large ones are opaque. The large plaques vary between 1–3 mm in diameter, and are over 15 cell layers thick. Each large plaque has a main central region and edges continuous with either the microscopic plaque or the simple amniotic epithelium. The main region shows four zones, namely, stratum basale, stratum spinosum, stratum granulosum and stratum corneum. Such zones are not distinct at the edges. The fine structure of basal cells compares with the amniotic epithelial cells, but the cells of spinosum and granulosum layers possess variable amounts of tonofibrils, keratohyalin granules, free ribosomes and other cytoplasmic organelles and inclusions. The corneum cells are keratinized and are frequently separated by intercellular spaces. They slough into the amniotic cavity singly or as a sheet, and contribute towards the composition of the amniotic fluid. The plaques are of amniotic origin, and are not formed by adhesion of either squamous cells or fetal skin cells (masses of keratinized squames). The present observations suggest that the occurrence of amniotic plaques is normal. The presence of plaques may not be necessarily associated with fetal abnormality. However, increase in numbers of plaques may be caused by conditions of fluid imbalance. The homology and significance of plaques in eutherian mammals have been discussed.
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  • 57
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    Cell & tissue research 126 (1972), S. 261-277 
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Rat ; Cell types ; Secretion ; Influence of fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Nebennierenmark der Ratte wurde nach Anwendung verschiedener Fixationsmethoden untersucht. Nach Immersionsfixation mit Glutaraldehyd oder Osmiumtetroxyd finden sich in der Nebenniere sog. Mischzellen, helle Zellen, syncytiale oder plasmodiale Zellen, die von den Untersuchern als Artefaktbildungen angesehen werden. In allen Proben nach Perfusionsfixation wurden solche Artefaktbildungen niemals beobachtet. Bei Immersionsfixation wurde eine enge Beziehung zwischen Sekretgranula und Cytoplasmamembran beobachtet, was bei Perfusionsfixation selten der Fall war. Die heutigen Theorien über den Sekretionsmechanismus bei Nebennierenmarkzellen werden aufgrund der vorgelegten Ergebnisse diskutiert. Die Arbeit zeigt die wesentlichen Vorteile der Anwendung der Perfusionsfixation für die Untersuchung der Nebenniere.
    Notes: Summary The adrenal medulla of the rat was studied utilizing various methods of fixation. In adrenal medulla specimens after immersion fixation either with glutaraldehyde or osmium tetroxide, elements such as mixed, clear, syncytial, or plasmodial cells, believed to be of artifactual origin, are observed in all of this material examined. These elements are absent in the specimens prepared by perfusion fixation. In specimens prepared by immersion fixation, secretory granules are found in close proximity to the plasma membrane; this localization is infrequent after perfusion fixation. Current theories of the mechanism of secretion of adrenal medullary hormones are discussed on the basis of our results. This investigation demonstrates the advantage and necessity of perfusion fixation in the study of the adrenal medulla.
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  • 58
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    Keywords: Neurones ; Lumbricus ; Monoamines ; Neurosecretion ; Electron microscopy
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    Notes: Summary The cerebral ganglion and the ventral nerve cord of Lumbricus terrestris have been studied with the electron microscope. The results are as follows: In the neuropile small granular vesicles (300 to 500 Å) occur in some varicose nerve fibres after fixation with potassium permanganate. This indicates the presence of noradrenaline. Sometimes only a few of the vesicles produce a positive reaction. After incubation with α-methyl-noradrenaline the numbers of nerve terminals with small granular vesicles greatly increase, indicating the presence of dopamine and/or 5-hydroxytryptamine. In this case the reaction is now complete. The number of small granular vesicles is largest in the terminal swellings. These findings are consistent with histofluorescence, chemical, and microspectrofluorometric analyses, which have demonstrated noradrenaline, dopamine, and 5-hydroxytryptamine in neurones in the central nervous system. Large granular vesicles (600 to 900 Å) are to be found in some perikarya, not identical with neurosecretory cell bodies. In this case the granular vesicles in the axon are smaller and fewer. This indicates a simultaneous proximo-distal transport and gradual decrease in size of the granular vesicles. The intraneuronal distribution of the vesicles is in agreement with the distribution of the fluorophores in the fluorescent neurones. Neurosecretory neurones are found most likely not to contain monoamines.
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  • 59
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    Cell & tissue research 126 (1972), S. 431-436 
    ISSN: 1432-0878
    Keywords: Golgi apparatus ; Granular endoplasmic reticulum ; Electron microscopy
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    Notes: Summary Numerous morphological continuities between the cisternae of the convex face of the Golgi apparatus and the granular endoplasmic reticulum (GER) were observed in post-division amebae that had divided following enucleation and renucleation. Electron microscopic radioautography with the use of 3H-uridine as a tracer indicated that perhaps the Golgi apparatus is derived from the GER. The possibility of the connections between GER and Golgi apparatus facilitating transport of materials between the two is also discussed.
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    Cell & tissue research 126 (1972), S. 466-482 
    ISSN: 1432-0878
    Keywords: Macrophages ; Microtubules ; Enamel organ ; Rat ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary In the enamel organ of rat incisors macrophages are present in the zone of matrix formation, the transitional zone, the enamel maturation and pigmentation zone. The macrophages accumulate adjacent to redifferentiating amelocytes in the transitional zone. The macrophages phagocytize fragments of disintegrating amelocytes. In addition to the well known complement of organelles the macrophages present an elaborated microtubular system, scattered, thick filaments, a cortical feltwork of thin filaments, and spherical nuclear bodies. The microtubules emanate from “attached” and free pericentriolar satellites and radiate aster-like towards the cell surface or into pseudopods or curve along the nuclear surface for long distances, often related to nuclear constrictions. It is suggested that the microtubular system plays a prominent role in directional movement of the macrophages. The cortical filaments, if contractile, may create the cytoplasmic flow necessary for the cell motility.
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  • 61
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    Cell & tissue research 127 (1972), S. 116-126 
    ISSN: 1432-0878
    Keywords: Cell junctions ; Nexus ; Osmotic effects ; Fixatives ; Electron microscopy
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    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Speicheldrüsen von Mückenlarven (Chironomus Thummi) haben eine ausgedehnte interzelluläre Kommunikation. In elektronenmikroskopischen Bildern der Speicheldrüsen wurden zwei Arten von interzellulären Verbindungen gefunden, die für die Zellkopplung verantwortlich sein könnten: septate junctions und close membrane junctions. Da die räumliche Ausdehnung der septate junctions viel größer zu sein scheint als die der close junctions, wurden erstere als wahrscheinliche Kopplungsstrukturen angesehen. Es gibt jedoch Hinweise, daß die Strukturen, welche die Zellkopplung bewirken, sehr labil sind. Unter den Faktoren, die zu einer Unterbrechung der zellulären Kommunikationen führen können, sind auch osmotische Effekte. Um mögliche Einflüsse dieser Art auf die Ultrastruktur zu verhindern, wurden die Drüsen für die mikroskopische Inspektion in isoosmotischen Lösungen fixiert. Unter diesen Bedingungen lassen sich ausgedehnte Membrankontakte vom nexus-Typ zwischen den Drüsenzellen erkennen. Ihre Ausdehnung scheint ebenso groß zu sein wie die der septate junctions. Es besteht nach diesen Befunden die Möglichkeit, daß wie in anderen kommunizierenden Zellsystemen, so auch in Speicheldrüsen die interzelluläre Kommunikation durch nexus bewirkt wird.
    Notes: Summary Cells ofChironomus salivary glands communicate through intercellular connections of high permeability. Electron micrographs of salivary glands show two kinds of junctions between the membranes of adjacent cells, which may be responsible for cell coupling: septate junctions and close membrane junctions. A large fraction of lateral cell surfaces is occupied by septate junctions, while the area of close membrane junctions appears to be very small. Consequently septate junctions have been considered as likely sites for intercellular coupling. There are however some indications that intercellular communication is provided by structures which seem to be unstable. As osmotic effects are among the factors which can disrupt cellular communications, we have tried to eliminate possible effects of the fixing solutions on the ultrastructure of intercellular connections by using isoosmotic fixatives. Under these conditions large regions of close membrane junctions of the nexus kind have been observed to occur between gland cells. They are of similar size as septate junctions. It seems to be possible that as in other communicating cell systems nexus could be the sites for intercellular coupling of salivary gland cells.
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  • 62
    ISSN: 1432-0878
    Keywords: Olfactory tubercle ; Rat ; Degeneration ; Synapse
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    Notes: Summary The olfactory tubercle of the rat was studied by electron microscopy both in the normal and after ipsilateral olfactory bulb ablation at survival times of from 14 hours to seven days. Particular emphasis was placed on synaptic structures and their changes following the lesion. Normal synapses are similar to those described in previous studies and presynaptic profiles are of at least three types. Types-A and -B contain round vesicles and form asymmetrical contacts and type-C profiles contain flattened vesicles and form symmetrical contacts. There appear to be two major types of degenerative changes. The electron-lucent type predominates at early survival times and is seen first at 14 hours. These profiles show an early reduction in numbers of vesicles with mitochondrial swelling followed by shrinkage of the profile. These profiles become increasingly electron-dense at later survival times. The second major type of degenerating profile is initially electron-dense. The earliest changes in these profiles are an increased axoplasmic density and increased microtubular density and clumping without apparent loss of vesicles. These profiles also become progressively more electron-dense at longer survival times. The observations are discussed in relation to previous reports.
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  • 63
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    Keywords: Synapses ; Crustacea ; Abdominal Ganglia ; Lateral glant fibers ; Electron microscopy
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    Notes: Summary The abdominal ganglia of the crayfish Astacus pallipes contain numerous vertebrate-like synapses which are characterized by presynaptic vesicles, darkened pre- and post-synaptic membranes, cleft material, and post-synaptic “fuzz”. Such synapses occur throughout the ganglia but are most easily found dorsally, where the neuropile is relatively coarse. The neuropile is far from homogeneous. Regional variations in fiber size, in degree of profile tortuosity, and in kind, magnitude, and distribution of vesicular content result in conspicuous textural variations. The structural polarity of synapses between the lateral giant fibers and other neurons is consistent with known physiological polarity and, hence, validates our criteria for recognition of synapses within the ganglion.
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    Cell & tissue research 128 (1972), S. 42-57 
    ISSN: 1432-0878
    Keywords: Microglia ; Pericytes ; Cerebral cortex (cat) ; Transformation ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Capillaries, pericytes and microglial cells in layer I of the cerebral cortex of normal adult cats have been studied with electron microscopy. The data obtained in this study show that pericytes are cells which are able to transform themselves into microglial cells by virtue of an activation process in which the astrocytic neuroglia appears to play a decisive role. By virtue of its structure, its mesodermic origin and its function the microglia has to be distinguished clearly from the astrocytic neuroglia and the oligodendroglia.
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  • 65
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    Cell & tissue research 128 (1972), S. 100-114 
    ISSN: 1432-0878
    Keywords: Connective tissue ; Gastropoda ; Globular cells ; Electron microscopy
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    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Blasenzellen stellen ein typisches Zellelement im Bindegewebe der Gastropoden dar. Licht- und elektronenmikroskopische Untersuchungen an Cepaea nemoralis haben gezeigt, daß der größte Teil einer Blasenzelle mit einer veränderlichen Glykogenmenge angefüllt ist. Diese zentrale Glykogenansammlung verdrängt das Zytoplasma mit seinen Organellen auf den peripheren Bereich der Zelle einschließlich der Zellausläufer und einen schmalen Saum um den Zellkern. Das wichtigste Identifizierungs-merkmal der Blasenzelle ist eine sehr spezialisierte — hier als Spaltenapparat bezeichnete — Oberflächendifferenzierung. Die Auswertung von Serienschnitten hat gezeigt, daß diese Oberflächenstruktur durch eine zum Teil verzweigte Invagination des extrazellulären Raumes gebildet wird, die wiederum von der Blasenzelle durch eine mäanderförmig unterbrochene Platte abgedeckt ist. Zwischen dem Spaltenapparat der Blasenzellen und dem Reusenapparat der Podozyten der Niere scheint eine Ähnlichkeit zu bestehen.
    Notes: Summary The globular cells are typical elements of the connective tissue of Gastropods. Light- and electronmicroscopic investigations of Cepaea nemoralis have shown, that these cells are filled with variable contents of glycogen, accumulated in the centre of the cell. This crowds the cytoplasm and the cell organelles into the peripheral area, including the cell processes and a narrow band surrounding the nucleus. The typical element of the globular cell is a special differentiation of the cell surface, the so-called “Spaltenapparat”. The three-dimensional organisation of the “Spaltenapparat” has been analysed by serial ultrathin sections. The reconstruction shows, that the “Spaltenapparat” consists of numerous branched invaginations of the extracellular space covered by very small, winding cell processes; there are tiny clefts between them. There appears to be some similarity between the “Spaltenapparat” of the globular cells and the pedicels of the podocytes of the renal glomerulus.
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    Cell & tissue research 128 (1972), S. 135-138 
    ISSN: 1432-0878
    Keywords: Spinal cord ; Mouse ; Myelinated neuronal soma ; Electron microscopy
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    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Thorakalmark (Hinterhornbereich) einer Wildmaus wurde ein kleines Nervenzellperikaryon beobachtet, das vollständig von einer Markscheide umhüllt war. Die Zahl der Markscheidenlamellen variierte zwischen 7 und 12. An einer Stelle konnte ein sogenanntes inneres Mesoperikaryon nachgewiesen werden. Die Bedeutung dieses zufällig erhobenen Befundes ist vorerst noch offen.
    Notes: Summary In the thoracic cord (posterior horn region) of a wild mouse, we have observed a small nerve cell soma completely enveloped by a myelin sheath. The number of myelin lamellae varied between 7 and 12. In one place, the existence of an inner ‘mesoperikaryon’ could also be shown. The significance of this fortuitous finding has not yet been explained.
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    Cell & tissue research 126 (1972), S. 53-74 
    ISSN: 1432-0878
    Keywords: Oogenesis ; Rhesus monkey ; Meiotic chromosomes ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary The ovaries of foetal and neonatal rhesus monkeys have been examined with the electron microscope. The fine structure of the germ cells (oogonia; oocytes at the preleptotene, leptotene, zygotene, pachytene and diplotene stages of meiotic prophase) closely resembles that of corresponding human cells. Stages in spontaneous atresia are also described. Cytoplasmic organelles in oogonia are sparse and are grouped mainly at one pole of the nucleus, but become dispersed and more abundant as oogenesis proceeds. The nuclei of oogonia contain a random fibrillar matrix which becomes organized into threads at pre-leptotene. At leptotene these chromosomal threads each contain a dense axial “core”; during zygotene they become loosely paired in a “bouquet” arrangement and at pachytene the bivalents contain synaptinemal complexes. “Single” cores reappear at diplotene, surrounded by a complex fibrillar sheath organized into lateral projections and loops with associated granules: such chromosomes resemble those in human primordial oocytes although they are more diffuse. These findings support the view that at the diplotene stage mammalian oocytes contain chromosomes of the lampbrush type. Observations on the monkey are compared with those on other species, and the ways in which chromosomal organization may influence the radiosensitivity of oocytes is discussed.
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    Cell & tissue research 129 (1972), S. 1-10 
    ISSN: 1432-0878
    Keywords: Spermatogonia ; Fish ; Annulate lamellae ; Chromatoid bodies ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Observations on annulate lamellae and chromatoid bodies in spermatogonia of the cyprinid fish Pimephales notatus have revealed several commonly occurring features heretofore unreported: These include (a) the presence of annulate lamellae in close association with chromatoid bodies; (b) the existence of a chromatoid “band” or “shell” between the nuclear envelope and some chromatoid bodies with connections among them; (c) the presence of annulate pore complexes in the absence of well developed membrane envelopes as well as in association with such envelopes; (d) the presence of material just outside the nucleus and contiguous with nuclear pores which is of a similar density and texture to that of the chromatoid bands and chromatoid bodies; (e) filamentous material between the cytoplasmic sides of nuclear pores and the chromatoid “band”, bridging a distance of approximately 1000 Å and similar threads extending a like distance between chromatoid bodies (and bands) and annulate lamellae associated with them; and (f) mitochondria closely arranged about some chromatoid bodies.
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    Cell & tissue research 127 (1972), S. 127-148 
    ISSN: 1432-0878
    Keywords: Nuclear envelope ; Amphibian oocytes ; Nuclear pore complex ; Chemical nature ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary In order to investigate the chemical composition of the nuclear pore complexes isolated nuclei from matureXenopus laevis oocytes were manually fractioned into nucleoplasmic aggregates and the nuclear envelopes. The whole isolation procedure takes no more than 60–90 sec, and the pore complexes of the isolated envelopes are well preserved as demonstrated by electron microscopy. Minor nucleoplasmic and cytoplasmic contaminations associated with the isolated nuclear envelopes were determined with electron microscopic morphometry and were found to be quantitatively negligible as far as their mass and nucleic acid content is concerned. The RNA content of the fractions was determined by direct phosphorus analysis after differential alkaline hydrolysis. Approximately 9% of the total nuclear RNA of the matureXenopus egg was found to be attached to the nuclear envelope. The nonmembranous elements of one pore complex contain 0.41×10−16 g RNA. This value agrees well with the content estimated from morphometric data. The RNA package density in the pore complexes (270×10−15 g/μ3) is compared with the nucleolar, nucleoplasmic and cytoplasmic RNA concentration and is discussed in context with the importance of the pore complexes for the nucleo-cytoplasmic transport of RNA-containing macromolecules. Additionally, the results of the chemical analyses as well as of the3H-actinomycin D autoradiography and of the nucleoprotein staining method of Bernhard (1969) speak against the occurence of considerable amounts of DNA in the nuclear pore complex structures.
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    Cell & tissue research 127 (1972), S. 50-86 
    ISSN: 1432-0878
    Keywords: Salivary glands ; Drosophila, larval ; Differentiation ; Involution ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Entwicklung bzw. Differenzierung der larvalen Speicheldrüse vonDrosophila melanogaster wurde an genau datierten Altersstadien aus dem III. Larvenstadium, der Vorpuppe und der Puppe mit lichtmikroskopischen und elektronenmikroskopischen Methoden untersucht. Zur Vermeidung großer Streuung im physiologischen Alter der Tiere wurde eine Kulturmethode entwickelt, die es erlaubt, die Häutungen zu beobachten und zur Altersbestimmung heranzuziehen. Folgende Ergebnisse wurden erzielt: 1. Die Speicheldrüse besteht bis zur Mitte des III. Larvenstadiums morphologisch aus einem einheitlichen Zelltypus, der sehr kleine Sekretgrana (∅ 0,3 μm) bildet. Diese sammeln sich am Zellapex. Die Vermutung liegt nahe, daß es sich um ein Verdauungssekret handelt.In der 2. Hälfte des III. Larvenstadiums differenzieren sich drei Zelltypen, die hier Corpuszellen, Übergangszellen und Halszellen genannt werden. Dabei ist ein Differenzierungsgradient von distal nach proximal zu beobachten. Die distal gelegenenCorpuszellen stellen die Bildung des Verdauungssekretes in der 2. Hälfte des III. Larvenstadiums ein und bilden stattdessen ein Klebesekret. Dieses Sekret wird in Form großer Grana (∅ bis zu 10 μm) zunächst in den Zellen gespeichert und kurz vor der Pupariumbildung ins Lumen der Drüse abgegeben. Kurz nach der Pupariumbildung wird das Klebesekret aus dem Körper entlassen und dient dazu, die Tönnchenpuppe an einem trockenen Substrat anzuheften. Das Klebesekret ist PAS-positiv. Wahrscheinlich handelt es sich um ein Mucoproteid. Während des Vorpuppenstadiums bilden sich in den Corpuszellen große Vakuolen, die auf Grund der elektronenmikroskopischen Befunde als Ausdruck einer weiteren Sekretionsphase und nicht als beginnende Degeneration gedeutet werden. Die mögliche Bedeutung dieses Sekretes wird diskutiert. DieÜbergangszellen liegen zwischen den Corpuszellen und den Halszellen. Sie bilden ebenfalls Klebesekret, jedoch mit zeitlicher Verzögerung. Kurz vor der Pupariumbildung sind sie wie die Corpuszellen mit ausgereiften Klebesekretgrana beladen und von diesen nicht mehr zu unterscheiden. Die proximal gelegenenHalszellen bilden kein Klebesekret, sondern setzen die Bildung des Verdauungssekretes in der 2. Hälfte des III. Larvenstadiums fort. Während des Vorpuppenstadiums bilden sich in den Halszellen nicht die großen Vakuolen wie in den Corpuszellen. 2. Die Involution der larvalen Speicheldrüse erfolgt nach der Puppenhäutung durch Autolyseprozesse, die am distalen Ende der Drüse beginnen und innerhalb 1 Std alle Zellen mit Ausnahme der Imaginalanlage erfassen. 3. Die in dieser Untersuchung erhobenen entwicklungsgeschichtlichen Befunde anDrosophila melanogaster werden mit Beobachtungen anDrosophila virilis, D. robusta undD. hydei verglichen. Dabei wird aufgezeigt, daß die Entwicklung der larvalen Speicheldrüsen von verschiedenenDrosophila-Arten enge Parallelen aufweist. Die bisher bekannten Zusammenhänge zwischen Stoffwechselaktivitäten im Zytoplasma und Genaktivitäten (Puffmuster) an den Riesenchromosomen dieser Zellen werden diskutiert.
    Notes: Summary The development and differentiation of the larval salivary glands ofDrosophila melanogaster have been investigated with light and electron microscopical methods. The organ has been dissected out of exactly dated stages of the III. instar larva, the prepupa and the early pupa. In order to avoid great variations in the physiological age of the animals a culture method has been developed, enabling the larval molts to be observed and used for identification of the age. The results are as follows: 1. The salivary gland of the early larva up to the middle of the III. instar period is a homogenous sack consisting of one sort of cells, in which very small secretion granules (∅ 0,3 μm) are synthesized. These secretion granules concentrate near the cellular apex. They are supposed to contain digestion enzymes. 2. In the second half of the III. larval instar period three cell types are differentiated, which are called corpus cells, transitional cells and collum cells. A gradient of differentiation from distal to proximal can be observed. 3. Thecorpus cells, located at the distal part of the gland, stop the production of digestion enzymes in the second half of the III. larval instar period and begin to synthesize a cement substance. This cement first is stored in grana (∅ up to 10 μm) inside the corpus cells. Shortly before puparium formation it is extruded into the lumen of the gland. Shortly after puparium formation it is expectorated out of the mouth, runs along the body wall and affixes the puparium to the substrate. The cement is PAS-positive, probably being a mucoproteid. In the corpus cells large vacuoles are formed during the prepupal instar period. On the basis of these electron microscopical results the vacuoles are interpreted to represent another form of a secretory product and not an equivalent of beginning degeneration. The possible function of this substance is discussed. 4. Thetransitional cells are located between the corpus cells and the collum cells. They also synthesize cement at a delayed rate, through shortly before puparium formation they are filled with cement like the corpus cells and cannot be distinguished from the latter. Thecollum cells form the most proximal part of the salivary gland. They do not produce cement but continue to synthesize digestion enzyme granules in the second half of the III. instar period. The large secretion vacuoles, found in the corpus cells during the prepupal instar period, are not synthesized in the collum cells. 5. The involution of the larval salivary gland begins after pupation and is indicated by autolytic processes, which begin at the distal end of the gland. One hour later all cells exept the imaginalanlage show signs of degeneration. 6. The course of development of the salivary glands investigated in the present study inDrosophila melanogaster is compared with similar investigations onDrosophila virilis, robusta andhydei. It is pointed out that the development of the larval salivary gland in different species ofDrosophila shows close parallels. The relationships between metabolic activities in the cytoplasm and gene physiological activities (pattern of puffs) on the giant chromosomes, as known so far, are discussed.
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    Cell & tissue research 127 (1972), S. 201-208 
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    Keywords: Spermatogenesis ; Rat ; Mitosis ; Ultrastructure
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    Notes: Summary The pattern of intercellular cytoplasmic bridges between rat spermatogonia and between spermatocytes is illustrated from electron microscopy of serial sections. Clones, or syncytia, containing as many as 22 connected spermatogonia and as many as 74 connected spermatocytes were observed. The absence of closed rings of cells agrees with the observation that intercellular bridges are the result of incomplete cell division, rather than cell fusion. The bridges thus are a record of spermatogonial divisions within a clone. In early spermatogonial generations there is a predominantly linear arrangement. The groups of spermatocytes have more side branches. From the presence of synaptonemal complexes it is concluded that the connected spermatocytes of a given clone are in about the same developmental stage. The pattern of intercellular bridges indicates, however, that not all nuclei in a clone undergo mitosis in the same cycle. The connected cells of a clone are therefore not all of the same generation. From unconnected bridges it is assumed that new clones originate from single cells or groups of spermatogonia which separate from an existing clone.
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  • 72
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    Cell & tissue research 130 (1972), S. 70-92 
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    Keywords: Oocytes ; Different types ; Dinophilus gyrociliatus ; Cytochemistry ; Electron microscopy
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    Description / Table of Contents: Zusammenfassung Dinophilus gyrociliatus bildet zwei Oocytentypen, einen größeren, aus dem ♀♀ hervorgehen (♀-Oocyte) und einen kleineren, der sich zu ♂♂ entwickelt (♂-Oocyte). Diese beiden Oocytentypen sind von frühen Stadien der Vitellogenese an durch ihr unterschiedliches Größenwachstum zu unterscheiden. Da bei Oogonien und prävitellogenen Oocyten keine zwei unterschiedlichen Zelltypen festzustellen sind, muß man annehmen, daß die Differenzierung in ♂- und ♀-Oocyten in einem zwischen der Prävitellogenese- und der Vitellogenesephase gelegenen Übergangsstadium beginnt. Während der Prävitellogenesephase finden Zellverschmelzungen statt, aber es konnten keine Beziehungen zwischen der Fusion von Oocyten und der späteren Differenzierung nachgewiesen werden. Die ♂-Oocyte beginnt schon auf einem frühen Stadium der Vitellogenese mit der Produktion von Mucopolysaccharid-Granula, die ♀-Oocyte erst später. Diese Granula bilden nach der Ablage der Eier die Ei- oder die Kokonhülle. Die ♀-Oocyte bildet größere Proteindottergranula als die kleinere ♂-Oocyte. Eine Trennung zweier Zellsorten nach Granulagrößen läßt sich schon auf dem Übergangsstadium durchführen. Der absolute RNS-Gehalt der reifen ♀-Oocyte liegt wesentlich über dem der ♂-Oocyte; dagegen ist die Konzentration der RNS in der ♂-Oocyte höher. Die RNS-Synthese verläuft in beiden Oocytentypen parallel zur Volumenzunahme und dauert bis zum Ende der Vitellogenesephase.
    Notes: Summary Dinophilus gyrociliatus produces two types of oocytes, a big, female producing “♀-oocyte”, and a smaller, male-producing “♂-oocyte”. They may be distinguished by their different volume from the beginning of the vitellogenic phase. Neither oogonia nor previtellogenic oocytes show two types of cells, and the beginning of differentiation in ♀-oocytes and ♂-oocytes has to be located in a connecting stage after the previtellogenic and before the vitellogenic phase. On previtellogenic stages the cells fuse and form bigger ones, but there is no connection to be found with the differentiation of the egg cells. The ♂-oocyte starts the production of mucopolysaccharid granules at an early vitellogenic stage; the ♀-oocyte does so only at later stages. These granules form the egg capsule after the eggs have been laid. The ♀-oocyte contains bigger protein yolk granules than the smaller ♂-oocyte. Already on the connecting stage it is possible to distinguish two groups of cells by the size of their granules. The ribonucleic acid content in the ♀-oocyte exceeds greatly that of the ♂-oocyte. The RNA-concentration, however, is higher in the latter one. During the vitellogenic stages the rate of RNA-synthesis in either type of oocytes parallels the increase in cell volume, the synthesis lasting up to the end of the vitellogenic phase.
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    Cell & tissue research 130 (1972), S. 171-179 
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    Keywords: Tubuli seminiferi ; Rat ; Structure ; Contractility ; Organ culture
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    Topics: Biology , Medicine
    Notes: Summary Isolated pieces of seminiferous tubules of adult rats were grown in organ culture for up to 8 weeks in Petri dishes on the surface of nutrient agar. The medium consisted of newborn calf serum, Eagle's minimum essential medium, glutamate and antibiotics. This method allowed observation of the contractions of the seminiferous tubules in the culture. Contractility, light and electron microscopic structure and histochemically demonstrable activities of alkaline phosphatase and ATPase of the tubule walls were studied at 1-week intervals. The contractility and alkaline phosphatase activity were maintained in the tubule wall for 3 weeks, and the activity of ATPase was maintained for 5 weeks. The thin filaments of the myoid cells, which are responsible for the contractility, were seen with the electron microscope in tubules cultured for 5 weeks. The organ culture method described in the present paper seems to be valuable for studies concerning the functioning of the myoid cells of the seminiferous tubules and the possibility that this is regulated by hormones.
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  • 74
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    Cell & tissue research 131 (1972), S. 99-107 
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    Keywords: Median eminence ; Rat ; Exocytosis ; Micro-pinocytosis ; Releasing factors
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    Notes: Summary Exocytosis has been demonstrated by electron microscopy in the external zone of the median eminence of the rat. Exocytotic profiles have been observed in nerve fibres characterized by the presence of granular vesicles with median diameters of 90–103 nm and agranular vesicles of about 50 nm. In addition to the small agranular vesicles, coated vesicles of the same size have been found in many nerve fibres, suggesting that at least part of the agranular vesicles in the median eminence originate by micro-pinocytosis. The nature of the fibres showing exocytosis is discussed. Attention is drawn to the possibility of identifying types of fibres in the median eminence by the occurrence of exocytosis.
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    Cell & tissue research 131 (1972), S. 417-427 
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    Keywords: Lymphatic vessels ; Lung ; Centrioles ; Filaments ; Electron microscopy
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    Notes: Summary An electron microscopic study of the pulmonary lymphatic collecting channels and their valves in the rabbit revealed that the endothelial cells generally contain two centrioles which are almost invariably associated with one to several striated bundles of filaments. The structure of the centrioles corresponds well with that in other cell types. The filaments however were present only in endothelial cells and not in the perilymphatic connective tissue cells. The bundles consist of 2 to 6 filaments of about 40 Å diamenter and show a cross banding with a periodicity of 600 to 900 Å. They are attached at both ends or in the middle of the centriole. Their function is unknown, but they might be vestigial rootlets of rudimentary cilia of lymphatic endothelial cells.
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    Cell & tissue research 132 (1972), S. 365-380 
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    Keywords: Golgi apparatus ; Membranes ; Cross-bridges ; Electron microscopy
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    Notes: Summary Electron opaque cross-bridge structures span the inter- and intracisternal spaces and provide membrane-to-membrane connections between adjacent cisternae of dictyosomes of pollen tubes ofClivia andLilium. Additionally, the classic intercisternal rods, characteristic of intercisternal regions near the maturing face of dictyosomes, are connected with the adjacent membranes through similar cross-bridge elements. We suggest that these structural links are responsible for maintaining the flattened appearance of the central parts of Golgi apparatus cisternac as well as for the coherence of cisternae within the stack. Observations on other plant (e.g. microsporocytes ofCanna) and animal cells (e.g. rodent liver and hepatoma cells, newt spermatocytes) show that such an array of membrane cross-links is a universal feature of Golgi apparatus architecture. The cross-bridges appear as part of the complex “zone of exclusion” which surrounds dictyosomes, entire Golgi apparatus and Golgi apparatus equivalents in a variety of cell types.
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    Cell & tissue research 132 (1972), S. 553-562 
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    Keywords: Basement membrane ; Brain ventricles ; Rat ; Subependymal labyrinths ; Light- and electron microscopy
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    Description / Table of Contents: Zusammenfassung Mit der Perjodsäure-Bisulfit-Aldehydthionin-Färbung (Specht) lassen sich am ganzen Ventrikelsystem des Rattengehirns sub- und interependymale Strukturen lichtmikroskopisch nachweisen, die elektronenmikroskopisch als Basalmembranlabyrinthe (BML) identifiziert werden können. Verschiedene Formen dieser BML lassen sich unterscheiden. Sie stehen in Verbindung mit subependymalen Kapillaren. Die Verteilung der BML wird in Ventrikelkarten dokumentiert.
    Notes: Summary Sub- und interependymal structures, being demonstrable by a period-acid-bisulfit-aldehydthionine-method (Specht), occur in the whole ventricular system by light microscopy. They can be identified electron microscopically as basement membrane labyrinths (BML). Different shapes of these BML can be distinguished. They are in communication with the basement membrane of subependymal capillaries. The distribution of BML is documented in “ventricular maps”.
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    Cell & tissue research 130 (1972), S. 243-248 
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    Keywords: Blood vessels ; Chicken embryo ; Multivesicular structures ; Electron microscopy
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    Description / Table of Contents: Zusammenfassung In verschiedenen Arten von Gefäß- und Mesenchymzellen in Allantois und Dottersack des Hühnchens wurden regelmäßig sog. „multivesicular structures“ beobachtet, die aus intrazellulären Aggregaten von Vesikeln (Durchmesser ca.: 800 Å) und/oder Vakuolen (Durchmesser bis 5000 Å) bestehen. Ähnliche Befunde anderer Autoren und die eigenen Ergebnisse sprechen dafür, daß es sich um das Substrat einer weitverbreiteten Zellreaktion handelt, die u.a. das morphologische Äquivalent der Sequestrierung eines z.B. im Zuge der Zelldifferenzierung überflüssig gewordenen Zytoplasmaanteils darstellt.
    Notes: Summary In the endothelial, the media and mesenchymal cells of the vessels in the chicken's allantoic and vitelline membranes “multivesicular structure” regularly occur. They consist of aggregated vesicles and/or vacuoles (diameters ranging from 800 Å to 5000 Å) and seem to present a rather common and widespread cellular reaction, that might serve as a mechanism to sequester those parts of the cytoplasm which became superfluous e.g. during cell differentiation.
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    Cell & tissue research 130 (1972), S. 338-350 
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    Keywords: Adenohypophysis ; Salmon ; Gonad maturation ; Cell types ; Electron microscopy
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    Notes: Summary The fine structure of the various hormone-producing cell types (with the exclusion of the prolactin cells) in the pituitary gland (pars distalis) of migratory sockeye salmon is described. All fish were in an advanced stage of sexual maturation. In the proximal pars distalis five cell types were distinguished: growth hormone cells, ACTH cells, gonadotrops, “vesicular cells”, and “chromophobe cells”. Gonadotrops were also found throughout the rostral pars distalis. A conspicuous feature of the gonadotrops was the presence of two kinds of secretory inclusions: small electron-dense granules (200–375 mμ) and large, relatively electron-translucent globules (400–2 000 mμ). The large vesicular cells, so called because of their conspicuous vesicular endoplasmic reticulum, were numerous and often appeared to contain some small granules. It is argued that they may represent a second type of gonadotropic cell, which, in earlier stages of gonad development, contains many granules but becomes largely degranulated near the time of reproduction when the other gonadotrops (“globular gonadotrops”) abound. The chromophobes, which were smaller and far less abundant than the vesicular cells, also appeared to contain small granules (120–280 mμ). They are probably thyrotrops.
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    Cell & tissue research 134 (1972), S. 205-225 
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    Keywords: Diabetes insipidus ; Rat ; Hypothalamo-neurohypophysial system ; Synthesis of hormones ; Light- and Electron microscopy
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    Notes: Summary The general ultrastructural features of the hypothalamo-neurohypophysial system in rats with hereditary hypothalamic diabetes insipidus (DI-rats, Brattleboro strain) are described. There is no decisively distinguishing difference between the neurons of the supraoptic and paraventricular nuclei. The neurons of both nuclei show signs of active protein synthesis. The perikarya of the neurons are markedly hypertrophic, the nuclei are large and the nucleoli prominent. In the cytoplasm there are numerous ribosomes, abundant rough-surfaced endoplasmic reticulum and extensive Golgi complexes. However, very few neurosecretory granules are to be seen. The axons of the hypothalamo-neurohypophysial tract are likewise enlarged and the paucity of neurosecretory granules is a striking feature also in the area of the tract. The majority of nerve endings in the posterior pituitary of DI-rats are devoid of neurosecretory granules. Microvesicles are abundant in the nerve endings and there are findings which suggest that microvesicles are involved either in endoor exocytosis. The signs of active protein synthesis and the concomitant paucity of neurosecretory granules are interpreted to imply transportation of the secretory proteins in an extragranular phase. The possible mode of release of the secretory proteins from the nerve endings and the role of microvesicles therein are discussed.
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    Cell & tissue research 131 (1972), S. 347-370 
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    Keywords: Spermatozoon ; Pleurodeles ; Acrosome ; Differentiation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'origine et la morphogenèse des différents éléments de l'acrosome du spermatozoīde dePleurodeles waltlii ont été suivies et décrites depuis le tout début de la spermiogenèse. La formation de la vésicule acrosomienne et son évolution en une coiffe acrosomienne se fait selon le schéma classique. Son extrémité apicale se différencie tardivement en un bouton terminal et un crochet. Les trois parties de la coiffe diffèrent dans leur composition et leur structure fine. Les volumineux et complexes éléments situés sous la coiffe acrosomienne: axe, baguette puis manchon périphérique et manchon moyen, sont dépourvus de polysaccharides. Leur origine est envisagées. Ils sont comparés aux éléments situés dans l'espace sous-acrosomien des spermatozoīdes des autres vertébrés.
    Notes: Summary The origin and the morphogenesis of the acrosome different parts ofPleurodeles spermatozoon, have been investigated and described from the early beginning spermiogenesis process. The acrosomal vesicle and acrosomal cap formation take place according to the classical scheme. The acrosomal anterior tip cap late differentiate in a blunt terminal knob and a hook. The three cap parts differ in their composition and fine structure. The large and complicated structure stretching under the acrosomal cap: axis, peripheral muff and middle muff, are devoided of polysaccharides; their origin is discussed. They are compared with the subacrosomal components lying in the other vertebrates spermatozoon subacrosomal space.
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    Cell & tissue research 132 (1972), S. 25-34 
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    Keywords: Neurohypophysis ; Rat ; Perivascular cells ; Neurosecretion ; Ultrastructure
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    Notes: Summary The purpose of this study is to investigate the ultrastructural features of perivascular cells as found in the neural lobe of the hypophysis. In particular, an inquiry was made into the nature of the relationship of such cells to neurosecretory fibers and endings. The latter, in fact, are often invaginated within the perivascular cells and enveloped by their processes; furthermore, they often reveal a certain number of empty granules as well as characteristics of degenerative nature. In the course of this study the localization of the perivascular cells has been investigated as well as that of their processes within the extensive interlobular network typical of the hypophysial neural lobe of rodents. Based on the data gathered, the hypothesis is put forward that the perivascular cells play an important role in the turnover of neurosecretory endings, both under physiological and experimental conditions, contributing thereby to the release of post-hypophysial hormones.
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    Cell & tissue research 135 (1972), S. 211-228 
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    Keywords: Osteoclasts ; Rats ; Bone resorption ; Lysosomes ; Electron microscopy
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    Notes: Summary Osteoclasts in metaphyses from young rats were systematically sectioned at different levels. Two types of osteoclasts were recognized. One type had no ruffled border while the other, and predominant type contained a ruffled border in a part of its length; some of the latter contained two ruffled borders. The closest contact between osteoclast and bone occurred at the level of the ruffled border and this bone under the border showed characteristic changes indicative of resorption. In some osteoclasts the ruffled border consisted of numerous slender cytoplasmic projections separated by very narrow spaces or channels while in other osteoclasts it was more open. The ruffled border was commonly surrounded by a transitional zone containing numerous thin filaments. The osteoclast usually had its greatest dimension at the level of the ruffled border and the cytoplasm here contained many bodies and vacuoles but a sparse endoplasmic reticulum. Away from the level of the ruffled border the cytoplasmic vacuoles and bodies were fewer while the endoplasmic reticulum was often more pronounced. Parts of the osteoclasts were usually situated close to a vessel. It is suggested that there is a correlation between the development of the ruffled border and the degree of bone resorption and that osteoclasts without a ruffled border are, at least temporarily, inactive with respect to bone resorption. The numerous cytoplasmic bodies, interpreted as lysosomes, are presumed to be important in the resorption process. The closely adjacent positioning of osteoclasts and vessels may facilitate the transport of resorption products to the blood.
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    Cell & tissue research 133 (1972), S. 82-102 
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    Keywords: Echinoidea ; Colour change ; Chromatophores ; Amoeboid movement ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
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    Description / Table of Contents: Zusammenfassung Für den physiologischen Farbwechsel bei Vertebraten und Evertebraten gilt die Vorstellung, daß eine Pigmentbewegung innerhalb einer formkonstanten Zelle stattfindet. Am Seeigel Centrostephanus longispinus wird nun der Nachweis einer amoeboiden Bewegung von Pigmentzellen geführt: Die Epidermis von Centrostephanus enthält große braune Chromatophoren, die bei Belichtung eine Pigmentdispersion, bei Verdunkelung eine Konzentration des Pigments zeigen. Die Chromatophoren sind außerordentlich stark verzweigte Zellen, deren Arme dicht mit Pigmentgrana erfüllt sind. Im geballten Zustand ist die allgemeine Zellform mehr oder weniger ovoid, wobei die Zellarme eingezogen und dicht um die Zellmitte angeordnet sind. Dispersion des Pigments wird hervorgerufen durch Ausstrecken der pigmentierten Zellarme in den Interzellularraum des umgebenden Gewebes. Innerhalb der Zelle werden filamentöse Elemente nachgewiesen, die vermutlich für die Zellbeweglichkeit verantwortlich sind. — Ferner wird der zelluläre Aufbau des Integuments beschrieben.
    Notes: Summary Rapid colour changes in vertebrate and invertebrate species are considered to be due to movement of pigment granules within pigment cells of constant shape. Evidence is presented in this study to show that an amoeboid movement of chromatophores occurs in the epidermis of the Echinoderm Centrostephanus longispinus. The epidermis in this species contains large brown chromatophores, which display a dispersion of pigment on illumination and its concentration on darkening. The chromatophores are extensively branched cells, and their branches are densely packed with pigment granules. In the state of pigment concentration, the shape of the cell is more or less ovoid, and the cell branches are drawn in and closely arranged around the cell centre. Dispersion is attained by a stretching out of the pigmented cell branches into the intercellular spaces of the surrounding tissue. Within the cell, filamentous elements, which may be functional in the motility of the pigment cell, can be demonstrated.—Additionally the cellular composition of the integument is described.
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    Cell & tissue research 135 (1972), S. 439-448 
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    Keywords: Neuromuscular junction ; Triturus ; Colchicine ; Axoplasmic flow ; Electron microscopy
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    Topics: Biology , Medicine
    Notes: Summary Repeated injections of colchicine into the forearms of normal adult newts led to the disappearance of microtubules within some nerves and a concomitant loss of specialized morphological features at the neuromuscular junction. Within 2 weeks, the postsynaptic folds decreased in height and number, became flattened and eventually disappeared. In addition, nerve terminals in drug-treated animals became separated from the muscle surface and were highly congested with masses of synaptic vesicles. The present findings show that colchicine has an effect on the structural integrity of the neuromuscular junction. These effects could be direct; secondary to retraction of the nerve from the muscle surface; or the result of interference with the proper transport and/or release of neurotrophic substances.
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    Cell & tissue research 135 (1972), S. 449-459 
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    Keywords: Insect brain ; Neurons ; Glia ; Electron microscopy
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    Notes: Summary The fine structure of the neural sheath, glial cells and nerve cells in the brain of adult male houseflies is described. The neural sheath is composed of neural lamella and perineurium. The neural lamella consists of an external lamina and collagen-like fibrils which are embedded in an amorphous matrix. The perineurial cells form a continuous layer around the brain. On their inner surface, perineurial cells form junctional complexes with glial cell processes. A cortical cellular layer composed of neurons and glial cells surrounds the centrally located neuropil. Three types of glial cells are identified. Glial cells differ in size and in relative development and distribution of organelles. Thin processes of glioplasm completely surround the cell bodies of the neurons. Five types of neurons are described. Most of the neurons are monopolar, a few are bipolar.
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    Cell & tissue research 135 (1972), S. 461-471 
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    Keywords: Centriole assembly ; Basal bodies ; Ciliogenesis ; Electron microscopy
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    Notes: Summary Electron microscopy of 16 to 18 day old chick tracheas revealed that procentrioles are present near the basal ends of the recently matured centrioles and basal bodies of both ciliating and ciliated cells. Cylinders 0.1 μ in outside diameter in which densely staining walls and a central axial filament can often be detected, are present between the mature centrioles and these procentrioles. These cylinders although somewhat shorter are morphologically similar to those found earlier in the same cells in the center of procentriole clusters. So far, only one procentriole has been found in association with each cylinder and only one cylinder in association with each mature centriole or basal body. Procentrioles up to 0.18 μ in length including some with singlet microtubules in their walls have been detected. Serial sectioning indicated that in some cells up to 8% of the mature centrioles and basal bodies were associated with a cylinder and a distinct procentriole. If these procentrioles were to mature they could provide additional basal bodies for cilia after the initial wave of centriole assembly and maturation has been completed.
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    Cell & tissue research 136 (1973), S. 85-96 
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    Keywords: Liver ; Lampetra ; Physiological biliary atresia ; Electron microscopy ; Histochemistry
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    Topics: Biology , Medicine
    Notes: Summary A morphologic and histochemical study was carried out on the liver of larval and adult lampreys at the optical and electron microscopic level. In the larva the liver is composed of blind ending single cell thick tubules of hepatocytes. The tubular lumina provided with microvilli are morphologically comparable with the canalicular lumens of the higher species of animals. The cytoplasm of the hepatocytes contains numerous inclusions with heterogeneous appearance and crystalline material. The biliary system is composed of numerous bile ductules and ducts. In the adult lamprey, the biliary system has disappeared. The hepatocytes loose their tubular arrangement and the characteristic differentiation of their biliary pole. In contrast to previous reports in the literature the presence of bile pigment in the adult lamprey liver could not be demonstrated with any histochemical technique.
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  • 89
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    Keywords: Interstitial cells, genesis ; Hen's ovary ; Electron microscopy
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    Description / Table of Contents: Zusammenfassung Für die Zwischenzellen des Hühnerovars gibt es embryonal und post-embryonal bindegewebige und epitheliale Quellen. Die bindegewebigen Mutterzellen sind Mesenchymzellen und Fibrozyten, die epithelialen Mutterzellen sind Wandepithelzellen der Markstranglakunen und Epoophorontubuli. Kriterien zur Unterscheidung dieser vierfachen Abstammungsmöglichkeiten der Zwischenzellen werden angegeben.
    Notes: Summary The interstitial cells of the hen's ovary have two sources: connective tissue mother cells, which are mesenchymal cells or fibrocytes, and epithelial mother cells, which are wall epithelial cells of the medullary cord lacunae or of the tubuli of the epophoron. Criteria are provided for differentiating this fourfold genesis of the interstitial cells.
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    Cell & tissue research 139 (1973), S. 101-148 
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    Keywords: Infundibulum ; Rat ; Neuro-glial synapses ; Neuro-Vascular contacts ; Corticotropin-Releasing Factor ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Die Gefäßfortsätze von ependymalen und subependymalen Zellen bilden in der subependymalen Zone und in der Zona interna des Ratteninfundibulum mit Nervenfasern kompakte neuro-gliöse Faserbündel, die sich in der Zona externa aufzweigen. 2. In allen Zonen des Infundibulum kommen zwischen den Nervenfasern und den Gefäßfortsätzen zahlreiche neuro-gliöse Synapsen vor. In den Gefäßfortsätzen fällt die hohe Zahl an Mikrotubuli sowie die zahlreichen, vielgestaltigen Einschlüsse auf. 3. In der subependymalen Zone sind die Interzellularspalten an bestimmten Stellen außerordentlich weit. Sie haben eine kanalartige Beschaffenheit und enthalten feine Bündel von Nervenfasern. 4. Von den lateralen Anteilen des Infundibulum her erreichen Dendriten von Ganglienzellen des Nucleus infundibularis die Mitte des Infundibulum. In dieser Region sind axodendritische Synapsen anzutreffen. 5. Morphometrische Analysen der Nervenfaserendigungen der Zona externa von Normaltieren zeigen, daß die prozentuale Verteilung der nach Granulagröße differenzierten Nervenfaserklassen für Mitte und Seite der Zona externa etwa gleich ist. Zwischen der Größe der Elementargranula und der Anschnittfläche der zugehörigen Nervenfasern besteht eine direkte Beziehung. 6. Die Nervenfaserendigungen erreichen die Basalmembran des perikapillären Raumes fast ausschließlich im Bereich von gefäßwärts gerichteten Vorwölbungen der Zona externa. Das Ausmaß, in dem Nervenfasern im Vergleich zu den Gefäßfortsätzen von Ependymund Gliazellen den perivaskulären Raum erreichen, ist medial weitaus größer als lateral. 7. Bei bilateral adrenalektomierten Ratten nimmt in bestimmten, vorwiegend lateral gelegenen Nervenfasern die Zahl und Größe der Elementargranula in Abhängigkeit von der Überlebensdauer zu. Dies dürfte auf eine verstärkte Synthese und Speicherung von Corticotropin-Releasing Factor in diesen Nervenfasern zurückzuführen sein. Gegenüber dem Normalbefund ist die neurohämale Kontaktfläche erheblich vergrößert. Der perivaskuläre Raum enthält zerfallene Nervenfaserteile, die durch Bindegewebszellen phagocytiert werden. Diese Veränderungen dürften durch eine unter Versuchsbedingungen verstärkte Wachstumstendenz der Nervenfasern in Richtung auf die Blutgefäße und durch eine Abschnürung der Nerven-faserendigungen ausgelöst werden.
    Notes: Summary 1. In the subependymal and internal zones of the rat median eminence nerve fibres and vascular processes of ependymal and subependymal cells form neuro-glial bundles. They branch in the external zone. 2. In all these three zones of the infundibulum numerous neuro-glial synapses are found between nerve fibres and vascular processes of glial cells. The vascular processes contain a high number of microtubules as well as polymorphous granular inclusions. 3. In certain regions of the subependymal layer the intercellular spaces are enlarged. They form channel-like spaces containing small bundles of delicate nerve fibres. 4. Nerve cells of the infundibular nucleus located in the lateral parts of the infundibulum send dendrites to the medial parts of the infundibulum. In this area axo-dendritic synapses are found. 5. For morphometric analysis, the nerve fibres of the external zone were classified according to the diameter of their granules. It is shown that in the different regions of the external zone the distribution of the various types of nerve fibre is similar. Moreover it can be seen that a direct correlation exists between the size of the sectional plane of a given nerve fibre and the size of the granules it comprises. 6. Nerve fibre endings abutting on the basement membrane of the pericapillary space are mostly found in bulb-like protrusions of the external zone. The extent to which nerve fibres reach the perivascular space—as compared with the vascular processes of ependymal and glial cells—is higher in the medial than in the lateral parts of the infundibulum. 7. In bilaterally adrenalectomized rats the number and diameter of elementary granules increases in nerve fibres located laterally. This increase is directly related to the survival time and may be due to an enhanced synthesis and storage of Corticotropin-Releasing Factor in these nerve fibres. Compared with the findings in untreated animals the neurohemal contact area is significantly enlarged. The perivascular space contains degenerating nerve fibres which are undergoing phagocytosis by connective tissue cells. It is assumed that these alterations are due to the increased growth of nerve fibres towards the vessels of the “Mantelplexus”, and that, following adrenalectomy, this excessive growth leads to a pinching off of nerve fibres.
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  • 91
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    Cell & tissue research 138 (1973), S. 75-83 
    ISSN: 1432-0878
    Keywords: Invertebrate ganglia ; Spisula solida ; ynapses ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An examination using the electron microscope was carried out on the visceral ganglion of the marine bivalve mollusc Spisula solida. A range of fixation, block staining and section staining technique was used to study the structure of chemical synapses. Phosphotungstic acid employed as a block stain specifically stained pre- and post-synaptic structures associated with the membrane at synapses as well as one class of granular vesicle. The specialised contacts were however shown to be rare and in many parts completely absent. Many axons, containing several types of vesicle, were shown to be varicose and it is proposed that they may function in a similar way to the unspecialised varicose terminals of vertebrate autonomic neurons. The role of membrane specialisations in intercellular adhesion is discussed. This study concludes that many synapses may be morphologically unidentified using present criteria.
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  • 92
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    Cell & tissue research 138 (1973), S. 171-186 
    ISSN: 1432-0878
    Keywords: Tooth germs ; Organ culture ; Differentiation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tooth germs from foetal rats of 17 days post-insemination were maintained in vitro for 12 days. Odontoblasts and ameloblasts differentiated and secreted their respective matrices in which mineralization occurred. The ultrastructure of the cells was qualitatively similar to that observed in normal development. Odontoblasts contained more lysosome-like bodies and were found to degenerate in some sites. Mantle dentine was formed but few von Korff fibres were observed. Calcospherites were rarely seen and the mineralizing front of dentine was predominantly linear, associated with numerous small early foci of mineral formation. Enamel showed prism formation associated with the Tomes' process of the ameloblast but some local disturbances in the pattern of enamel formation were observed.
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  • 93
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    Cell & tissue research 138 (1973), S. 273-282 
    ISSN: 1432-0878
    Keywords: Nucleoli ; Type ; Influence of histone ; Tissue culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The frequency of various types of nucleoli was investigated in tissue cultures of human embryonal lung and HeLa cells cultured in the presence of calf thymus histone. The nucleolar morphology and the frequency of various nucleolar types were dependent on the concentration of histone in the tissue cultures of the human embryonal lung cells. HeLa cells required longer cultivation with histone to manifest some effect on nucleoli. In both cases, the observed nucleolar changes suggest the depression of nucleolar RNA synthesis.
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  • 94
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    Cell & tissue research 138 (1973), S. 261-272 
    ISSN: 1432-0878
    Keywords: Extrarenal blood vessels ; Rat ; Adrenergic nerves ; Fluorescence microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic innervation of the extrarenal blood vessels of the rat left kidney was investigated by fluorescence histochemistry and by electron microscopy. The trunk of the renal artery proximal to the aorta is elastic and appears to be very sparsely innervated. In contrast, near the kidney the renal artery—which divides into 3 to 4 large branches of the muscular type possesses a dense adrenergic innervation. The adrenergic terminal axons are situated in the adventitia close to the external elastic lamella, but only rarely in close contact with smooth muscle cells. In most instances several terminal axons are grouped and enclosed by a Schwann cell, single axons being rare. All terminal axons are able to take up and to store 5-hydroxydopamine which strongly suggests that they are adrenergic. The innervation of the renal vein is more sparse than that of the muscular arteries but somewhat denser than that of the elastic artery. In addition, close to the origin of the renal artery the presence of “small intensively fluorescent” (SIF) cells as well as of some adrenergic ganglion cells is noted. The latter are situated in the adrenergic nonterminal axon bundles, which run parallel to the blood vessels. It is concluded that the uneven adrenergic innervation along the artery as well as individual variations in the branching of the artery are the main causes of the unusually high individual variations of the NA content of this organ such as used in pharmacological experiments.
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  • 95
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    Cell & tissue research 138 (1973), S. 295-298 
    ISSN: 1432-0878
    Keywords: Cornea epithelium ; Precorneal film ; Domestic fowl ; Ruthenium red ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das vordere Corneaepithel des Haushuhnes besteht aus 5 Schichten. Die oberflächlichste Lage hat einen Zottenbesatz. Die 0,2 μ langen Mikrovilli haben eine Dicke von 0,04–0,05 μ und einen Abstand von höchstens 0,1 μ voneinander. Auf der freien Oberfläche liegt ein mit Rutheniumrot anfärbbarer Film von Glykosaminoglykanen von 0,26–0,3 μ Dicke. Die Mikrovilli ragen in diesen Film hinein. Dieser Film ist für den spiegelnden Glanz der Cornea verantwortlich. Die Tränensekretion kann sehr gering sein, weil ein Teil der Flüssigkeit an die sauren Mukopolysaccharide gebunden ist. Die Moleküle dieses Films werden wahrscheinlich in der oberen Zellage synthetisiert.
    Notes: Summary The anterior cornea epithelium of the house-hen consists of 5 layers. The outermost layer has a villus border. The 0.2 μ long microvilli are 0.04–0.05 μ thick and are not more than 0.1 μ apart. A film of ruthenium red stainable glycosamine Glycans of 0.26–0.3 μ thickness lies on the free surface. The microvilli protrude into this film. This film is responsible for the reflecting shine of the cornea. The tear secretion can be kept at a low level as a part of the fluid is bound to the acid mucopolysaccharides. The molecules for this film are probably synthesized in the outermost cell layer.
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  • 96
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    Cell & tissue research 138 (1973), S. 345-370 
    ISSN: 1432-0878
    Keywords: Axonal migration ; Catecholamines ; Sciatic nerve ; Radioautography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The radioautographic technique has been used to study the axonal migration of catecholamines in sympathetic fibres of the sciatic nerve of rats after ligature. A first series of experiments aimed at ascertaining the capacity of the proximal portion of adrenergic fibres to take up and store exogenous tritiated catecholamines (3H-DOPA; 3H-DA and 3H-NA) 3 to 22 hours after ligation. The results are qualitatively similar in rats pretreated and non-pretreated with IMAO, but the intensity of the radioautographic reaction is lower in the latter cases. Most of the labeled axons are filled mainly with vesicular and tubular profiles of endoplasmic reticular origin, large dense bodies (probably lysosomes) and/or mitochondria. The silver grains are generally superimposed on the vesicular and/or the tubular profiles, but in some cases on the large dense bodies, suggesting that exogenous catecholamines can be stored in lysosomes. The question whether SGV specifically store catecholamines also in the modified adrenergic fibres has been investigated in KMnO4 fixed material. These results show that beside a large number of fibres in which there is a strict correlation between labeling and SGV, some fibres containing SGV do not retain the 3H-NA. Conversely some fibres which contain mainly agranular vesicles display radioautographic reaction. Therefore, in case of ligated fibres, SGV cannot be considered the specific organelles for storage of catecholamines. The axonal migration of labeled catecholamines has been studied in animals pretreated with IMAO. A moderate, but selective, labeling is present in the proximal portion of sciatic fibres of rats in which administration of labeled catecholamine preceeded of 2 hours the ligature and this was performed 22 hours before fixation. From these combined types of experiments, it is concluded that despite the presence of all the structures necessary for the storage of a high amount of catecholamines in the modified adrenergic fibres, only a small fraction of catecholamines accumulated above the ligature has been transported by axonal migration. Therefore, the axonal migration of catecholamines appears as an epiphenomenon related to the distal migration of enzymatic and storage proteins from the perikaryon.
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  • 97
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    Cell & tissue research 136 (1973), S. 447-460 
    ISSN: 1432-0878
    Keywords: Spermatids (rat) ; Manchette ; Microtubules ; Influence of Colcemid ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Links unusual for their length and variable morphology have been described between manchette microtubules in late stages of rat spermiogenesis. In earlier stages of rat spermiogenesis obvious links longer than ∼160 Å are rare, the majority being ∼80 or ∼120 Å in length. The most easily discernible geometric pattern in cross-sections of assemblies of manchette microtubules in intermediate stages of rat spermiogenesis is that of linear arrays sometimes resulting in long and irregularly folded chains of closely linked microtubules. Colcemid disrupts these arrays and is responsible for the formation of more complex geometric patterns. Six hours after drug administration the manchette is dramatically reduced in length. Sheet-like links of variable dimensions and 〉160 Å in length interconnect not only microtubules but C-type microtubules as well as other links. These links are similar in morphology to those found in later stages of rat spermiogenesis. It is suggested that the formation of these links may perhaps be dependent upon aspects of microtubule disassembly.
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  • 98
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    Cell & tissue research 139 (1973), S. 463-471 
    ISSN: 1432-0878
    Keywords: Gallbladder (Human) ; Mucus secretion ; Reverse pinocytosis ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mucous secretory activity of the human gallbladder epithelium was investigated by light and electron microscopy and with histochemical techniques. There are two types of granules in the supranuclear region of the epithelial cells. The one low in density contains a fine filamentous material and gives a strongly positive silver methenamine reaction. The other is dense and only faintly positive. The granules of the former are considered to be mucous secretory granules and the granules of the latter may be lysosomes. PAS positive granules correspond presumably to both types of granules mentioned above. The mucous secretory granules are considered to be synthesized by the Golgi apparatus and the granular endoplasmic reticulum as has been confirmed in other mucous secretory cells. Their content is released from the cell by reverse pinocytosis. Typical goblet cells occur frequently in the fetal epithelium, but cannot be observed in the adult specimens.
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  • 99
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    Cell & tissue research 137 (1973), S. 421-434 
    ISSN: 1432-0878
    Keywords: Giant mitochondria ; Transitional epithelium ; Urinary bladder (mole) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Harnblasenepithel des Maulwurfs wurde elektronenmikroskopisch untersucht. Bei bestimmten Tieren finden sich in den Intermediärzellen „matrixreiche“ Riesenmitochondrien. Diese Riesenmitochondrien scheinen aus normalen Mitochondrien hervorzugehen und enthalten verschieden geformte Einschlüsse. Andere Organe und Zellen bei Maulwürfen mit Riesenmitochondrien in den Intermediärzellen des Harnblasenepithels weisen normale Mitochondrien auf. Es handelt sich demnach hier um eine sog. „Mitochondriose der Intermediärzellen“. Bedeutung, Bildung und Abbau der Riesenmitochondrien werden diskutiert.
    Notes: Summary Giant mitochondria containing an enormous matrix volume were found in the intermediary cells of the urinary bladder transitional epithelium of the mole. Regularly shaped mitochondria develop club-like protrusions forming giant mitochondria that contain several types of inclusions. Various other tissues were examined, but we could state that giant mitochondria exist only in the intermediary cells of the urinary bladder epithelium. Thus a peculiar “intermediary cell mitochondriosis” is described in this paper, not regularly occurring in the mole. The functional significance of the giant mitochondria is discussed.
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  • 100
    ISSN: 1432-0878
    Keywords: Annulate lamellae ; Annelidae ; Oogenesis ; Endocrinology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'évolution en l'absence d'hormone cérébrale provoque, dans les ovocytes de N. diversicolor, la formation de lamelles annelées cytoplasmiques (L.A.). Le processus débute par l'apparition de lames de réticulum à proximité du noyau (15 jours d'évolution à 15° C). Les lames se rapprochent ensuite du noyau et des pores apparaissent, situés le plus souvent face à ceux de l'enveloppe nucléaire (20ème jour). La différenciation des lames du réticulum en L.A. progresse ensuite depuis les éléments les plus proches du noyau vers les plus externes. Dans le cytoplasme des ovocytes ayant évolué à la température de 20° C, on assiste en outre à la formation d'empilements de citernes ergastoplasmiques. Ces citernes, non situées à proximité du noyau, subissent également une transformation progressive en L.A. L'étude cytochimique effectuée par digestion enzymatique a montré que la pepsine dégrade fortement le matériel dense des lamelles annelées. Ce matériel semble donc renfermer une teneur élevée en protéines auxquelles pourrait se trouver associé du RNA.
    Notes: Summary Experimental elimination of brain activity leads to the formation of cytoplasmic annulate lamellae (L.A.) in oocytes of N. diversicolor. This process begins with the appearance of cisternae of endoplasmic reticulum in the vicinity of the nucleus (15 days of development at 15° C). Then, the cisternae move closer to the nucleus and pores appear, often facing the pores of the nuclear envelope (20th day). The differentiation of endoplasmic reticulum into L.A. progresses from the elements closest to the nucleus to the more peripheral ones. In the cytoplasm of oocytes which developed at 20° C, stacks of cisternae of rough endoplasmic reticulum have been observed. These cisternae, which are not localized near the nucleus, also undergo a progressive transformation into annulate lamellae. A cytochemical study, based on enzymatic digestion, shows that pepsin strongly digests the dense material of L.A. This material, therefore, seems to contain a high amount of proteins, perhaps associated with RNA.
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