ISSN:
1432-203X
Keywords:
Key words Somaclonal variation
;
Picea glauca
;
RAPD
;
Somatic embryogenesis
;
Cryopreservation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Trees were regenerated from six white spruce embryogenic clones after cryopreservation for 3 and 4 years, respectively. Genetic stability was evaluated using randomly amplified polymorphic DNA (RAPD) fingerprints. Somaclonal variation was detected in some in vitro embryogenic cultures 2 and 12 months after they were re-established following cryopreservation but not in the corresponding regenerated trees. These results suggest that trees regenerated from cryopreserved cultures in subsequent years are primarily genetically stable in the genomic regions tested and that variation observed due to the in vitro culture process infrequently affects trees regenerated from normally maturing and germinating somatic embryos. However, trees regenerated from somatic embryos that matured or germinated abnormally in in vitro culture exhibited altered RAPD fragment patterns.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050689
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