Publication Date:
1990-08-17
Description:
A partial complementary DNA was isolated for a gene (rrg) that is normally expressed in mouse NIH 3T3 cells, but is down-regulated after cellular transformation by long terminal repeat (LTR)-activated c-H-ras (LTR-c-H-ras). This gene was reexpressed in a nontumorigenic persistent revertant cell line created by prolonged treatment of the transformed cells with mouse interferon alpha/beta. Persistent revertants stably transfected with rrg complementary DNA antisense expression vectors appeared transformed, had decreased amounts of rrg messenger RNA, and were tumorigenic in nude mice. Stable transfection with sense constructs did not alter the normal morphology, message level, or nontumorigenicity of the persistent revertant cell line.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Contente, S -- Kenyon, K -- Rimoldi, D -- Friedman, R M -- R01 CA 37351-04A1/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1990 Aug 17;249(4970):796-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1697103" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line, Transformed
;
Cloning, Molecular
;
DNA/genetics
;
*Gene Expression
;
*Genes, ras
;
Humans
;
Interferon Type I/pharmacology
;
Mice
;
Mice, Nude
;
Neoplasms, Experimental/etiology
;
Nucleic Acid Hybridization
;
Proto-Oncogene Proteins/*genetics
;
Proto-Oncogene Proteins p21(ras)
;
RNA/analysis/genetics
;
RNA, Antisense
;
RNA, Messenger/genetics
;
Rats
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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