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  • 1
    Monograph available for loan
    Monograph available for loan
    Leiden : Backhuys Publ.
    Call number: M 09.0014
    Type of Medium: Monograph available for loan
    Pages: VI, 565 S. , Ill., graph. Darst., Abb.
    ISBN: 9073348676
    Classification:
    Ecology
    Location: Upper compact magazine
    Branch Library: GFZ Library
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  • 2
    ISSN: 1432-2285
    Keywords: Cambial differentiation ; Glucose-6-phosphate dehydrogenase ; 6-Phosphogluconate dehydrogenase ; NAD kinase ; Pyridine nucleotides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Despite the importance of the vascular cambial differentiation, little is known about its regulation. In order to address this problem we attempted to biochemically characterize differentiating xylem and phloem elements during the early stages of development. By applying techniques of quantitative histochemistry we show that the total pool size of pyridine nucleotides is similar in the phloem (PD) and xylem (XD) oriented derivatives of the cambial zone of trees ofRobinia pseudoacacia L. Within the PD zone, the amount of NAD + NADH exceeded that of NADP + NADPH [around 600 versus 200 pmol (mg dry weight)-1], possibly indicative of a preponderance of catabolic pathways (ratio of NADH∶NAD about 1). In contrast, the NADP(H) system dominated in the XD zone. This coincided with a high activity of NAD kinase. In addition, the extractable activities of the key enzymes of the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, were greatly increased. At a ratio of NADPH∶NADP of approximately 1, this could be indicative of increased rates of reductive biosyntheses, and could thus well be involved in early steps of the formation of phenols and lignin monomers. Taken together, this first approach clearly shows that phloem-oriented and xylem-oriented cambial descendents exhibit distinct differences in their biochemical patterns even in early stages of differentiation.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2285
    Keywords: Acyllipids ; Heartwood formation ; Phospholipids ; Robinia pseudoacacia L. ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The radial distribution of membrane and storage lipids was determined in the trunkwood ofRobinia pseudoacacia L. The trees were felled in November at the time of heartwood formation and fluctuations in the amount and composition of phospholipids, free sterols, steryl esters, diand triacylglycerols, and free fatty acids were investigated across the sapwood-heartwood boundary. The individual compounds were identified and quantified by thin layer chromatography, enzymatic and colorimetric assays, and by capillary gas chromatography. Phospholipids show a significant decrease towards the boundary area, and in the heartwood only trace amounts can be detected. The same pattern is observed for free sterols in the sapwood; in the heartwood, however, they reach maximum values with increasing depth of the trunk. Steryl esters exhibit a complementary behaviour by accumulating at the periphery of the heartwood. No concentration changes are found in the total amounts of diacylglycerols and free fatty acids. In contrast, the triacylglycerol concentration declines steadily across the trunk. With regard to qualitative composition, free fatty acids and the fatty acid moieties of the esterified constituents vary in their chain length from 14 to 24 carbon atoms and have up to three double bonds. A radial gradient in the ratio saturated/unsaturated fatty acids can be observed: except for the phospholipid fraction the relative amounts of unsaturated fatty acids increase in centripetal direction. Seven phospholipids were identified: phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, and phosphatidic acid, which constitutes the major proportion. In the sterol group, sitosterol is the most abundant component. The composition of the esterified sterols remains constant across the trunk cross-section, whereas the relative frequencies of individual free sterols change markedly.
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  • 4
    ISSN: 1432-2285
    Keywords: Acylesterhydrolase ; Heartwood formation ; Lipase ; Robinia pseudoacacia L. ; Phospholipases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The radial profile of lipase and phospholipase activities was determined in the trunkwood ofRobinia pseudoacacia L. The trees were felled in November at the time of heartwood formation and alterations in the enzymatic activities were investigated across the sapwood and heartwood. Methods employed include gaschromatographic, colorimetric and enzymatic assays. On a dry weight basis, the hydrolysis of the artificial substrate pnitrophenylpalmitate shows a maximum activity in growth ring 4; however, the assay has proved not to be specific for lipase. In contrast, lipase analyses (triacylglycerol acylhydrolase; E.C. 3.1.1.3) with an authentic substrate show activity peaks in growth rings 1 and 4. With protein as a reference the highest activity is found in growth ring 5. A similar tendency is observed for phospholipase A1 (E.C. 3.1.1.32) and phospholipase A2 (E.C. 3.1.1.4). Phospholipase C (E.C. 3.1.4.3) activity decreases towards the sapwood-heartwood boundary; negligible traces of activity are detected in the heartwood, whereas, based on the protein content, growth ring 4 yields maximal activity. Phospholipase D (3.1.4.4) exhibits the same radial pattern with regard to protein content as a reference. On a dry weight basis there is a significant increase within the sapwood area, while in the heartwood the activity drastically decreases. The enzyme activities are discussed in relation to degradative processes within the plasma membranes and the hydrolysis of reserve lipids during heartwood formation.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 74 (1967), S. 1-17 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Durch eine Kombination von mikroautoradiographischem Chloridnachweis und elektronenoptischer Lokalisierung von Silberfällungen wurde versucht, die intracelluläre Verteilung des Chlorids und den Weg des Ions im Blatt zu verfolgen. Dabei ergaben sich unter anderem folgende Ergebnisse: 1. Es konnte ein Auftreten des Chlorids in Chloroplasten sichergestellt, im Zellkern wahrscheinlich gemacht werden. 2. Es ergaben sich Hinweise für einen apoplastischen und eine symplastischen Chloridtransport. 3. Für einen Konzentrationshub des Ions zwischen dem Plasma der Parenchymzellen und dem der Drüsenzellen konnten keine Anhaltspunkte erhalten werden.
    Notes: Summary Using a combination of microautoradiographic detection of chloride and an electronmicroscopic localisation of silver precipitation an attempt was made to elaborate the intracellular distribution of chloride and the pathway of this ion in the leaf. The main results were as follows: 1. It was shown that chloride occurs in the chloroplasts and probably also in the nuclei. 2. There are indications for an apoplastic and a symplastic transport of the chloride. 3. The findings do not suggest a rise of the chloride concentration in the cytoplasm between the parenchyma and the gland cells.
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  • 6
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung 1. Es wird eine Methode zur Ermittelung der Anfangs-Reaktionsgeschwindigkeit von Enzymen angegeben. 2. In der Abhängigkeit der Aktivitätssteigerung der NADP+-abhängigen GPD von verschiedenen Bestrahlungsstärken erwies sich Lemna gibba — ähnlich wie früher Ceramium rubrum — als an sehr hohe Lichtintensitäten angepaßt. 3. Für den zeitlichen Verlauf der Aktivitätssteigerung des Enzyms in Abhängigkeit von der Bestrahlungsstärke erhält man ähnliche Kurven wie für die entsprechende Abhängigkeit der Photosyntheseintensität. 4. Die Aktivität der NAD+-spezifischen GPD nimmt in Extrakten von Lemna ab, wenn die Pflanzen belichtet werden; sie verhält sich also dem NADP+-abhängigen Enzym entgegengesetzt. Es gibt Hinweise dafür, daß beide Enzyme reversibel ineinander umgewandelt werden können.
    Notes: Summary 1. A method for determination of the initial rate in enzymic non-linear progress reactions is described. 2. The relation between the increase in the activity of the NADP+-dependent glyceraldehyde-3-phosphate-dehydrogenase (GPD) and the different light intensities shows that Lemna gibba is adapted to high light intensities. 3. The time-curves for the increase in the activity of the enzyme in dependence on the different light intensities are similar to the corresponding curves for the intensity of photosynthesis. 4. The activity of the NAD+-dependent GPD in the extract decreases after preceding illumination of the plants; this activity is thus inverse to that of the NADP+-specific enzyme. There are indications (also in the literature) that the two enzymes are interconvertible.
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  • 7
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung 1. Tetrasporophyten, männliche und weibliche Gametophyten von Ceramium rubrum unterscheiden sich im Protein- und Pigmentgehalt, in der Aldolaseaktivität und in dem Ausmaß der Aktivitätssteigerung der NADP+-abhängigen Glycerinaldehyd-3-phosphat-Dehydrogenase durch bestimmte Bestrahlungsstärken. 2. Erst sehr hohe Bestrahlungsstärken führen zu einer Verringerung der Lichtaktivierung der NADP+-abhängigen GPD; Ceramium rubrum aus Flachwasser erweist sich somit als eine ausgesprochene Starklichtpflanze. 3. Die Aldolaseaktivität im Extrakt wird durch vorhergehende Bestrahlung der Algen nicht beeinflußt. 4. Die Aktivitätssteigerung der GPD zeigte eine ähnliche Abhängigkeit von der Lichtqualität wie die Photosyntheseintensität; die Phycobiline sind auch hier wirksam. 5. Versuche mit “Minusfiltern”, in denen aus Weißlicht bestimmte Wellenlängen weggefiltert wurden, ergaben keine Änderung der Enzymaktivierung, wenn Rotlicht im Bereich der Chlorophyll-Absorption, eine Hemmung, wenn Grünlicht, und eine beträchtliche Steigerung, wenn Blaulicht (463 nm) weggenommen wurde.
    Notes: Summary 1. Tetrasporophytes as well as male and female gemetophytes of Ceramium rubrum show differences in the protein and pigment content, in the activity of the aldolase, and in the light-induced increase in the activity of the NADP+-dependent glyceraldehyde-3-phosphate-dehydrogenase (GPD). 2. A very high light intensity is necessary to diminish the increase of the GPD-activity; our Ceramium-material (from shallow water) thus behaves like a strong-light plant. 3. The activity of aldolase in the extract is not influenced by the preceding illumination of the algae. 4. The increase in activity of the NADP+-dependent GPD depends on the light quality in a similar way as the intensity of photosynthesis; the phycobilins are also effective as light acceptors in this case. 5. Certain wave-lengths were removed with “minus-filters” from a continuous spectrum (white light). Absence of red-light (663, 714 nm) had no influence on the activation of the enzyme, absence of green light (543, 492) diminished the activating effect, and absence of blue light (463 nm) increased it in comparison to the control (white light).
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Planta 69 (1966), S. 111-123 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung 1. Eine chlorophyllfreie Mutante von Pisum sativum zeigte im Gegensatz zu der grünen Normalform bei Belichtung keine Aktivitätszunahme der NADP+-abhängigen Glycerinaldehyd-3-phosphat-Dehydrogenase. 2. Der Dunkelwert des Enzyms war bei beiden Erbsenformen etwa gleich hoch. 3. DCMU, ein Hemmstoff des nichtcyclischen Elektronenflusses in der Photosynthese, hemmt die lichtinduzierte Neusynthese des Enzyms bei einer Konzentration von 10-6 m um etwa die Hälfte, in höheren Konzentrationen vollständig. Die Dunkelwerte der Enzymaktivität werden nicht beeinflußt. 4. Salicylaldoxim blockiert ebenfalls—mit zunehmender Konzentration immer stärker—die Enzymsynthese im Licht. Die Hemmung ist bei einer Konzentration von 5 · 10-4 m vollkommen. 5. Durch 10-3 m Na-Azid werden bei Lemna die Photosynthese und die lichtinduzierte GPD-Synthese praktisch vollständig gehemmt, die Atmung um mehr als die Hälfte reduziert, der Dunkelwert der GPD-Aktivität aber kaum verringert. 6. 2,4-Dinitrophenol steigerte in niedriger Konzentration (10-6 m) den Sauerstoffverbrauch im Dunkeln stark, die Photosynthese schwach; die Lichtsynthese des Enzyms war nicht beeinträchtigt, der Dunkelwert unbeeinflußt. Bei einer Konzentration von 10-5 m war die Atmung schwach, die Photosynthese stärker vermindert; der Dunkelwert der GPD war dabei gegenüber den Kontrollen nicht verändert, die Lichtsynthese merklich verringert. Durch eine Konzentration von 10-4 m wurden die Atmung, die Photosynthese und die lichtinduzierte GPD-Synthese vollständig gedrosselt, während der Dunkelwert des Enzyms auf etwa die Hälfte herabsank. 7. Es wird gefolgert, daß die reversible Synthese der NADP+-abhängigen GPD im Licht vom Chlorophyll und der funktionierenden Photosynthese abhängig ist, wobei der Lichtreation II, dem nichtcyclischen Elektronenfluß, die entscheidende Rolle zukommt.
    Notes: Summary 1. A chlorophyll-less Pisum sativum mutant showed — in contrast to the normal form — no increase in the activity of the NADP+-dependent glycerinaldehyde-3-phosphate-dehydrogenase following illumination. 2. In both forms of Pisum the amount of the enzyme present in the dark was about equal. 3. DCMU, an inhibitor of the noncyclic electron flow in photosynthesis reduces the light-induced synthesis of the enzyme to about half in a concentration of 10-6 m and inhibits it totally at higher concentrations. The dark values of enzyme activity were not influenced by the presence of the inhibitor. 4. Salicylaldoxime also blocks the enzyme synthesis in the light; this effect increases with increasing concentration of the inhibitor. The inhibition is complete at a concentration of 5 · 10-4 m. 5. Sodium azide in a concentration of 10-3 m inhibits the photosynthesis and the light-induced GPD-synthesis almost completely; respiration is diminished by more than 50% whereas the dark value of the GPD-activity remains unchanged. 6. 2,4-Dinitrophenol in a low concentration (10-6 m) increased the oxygen consumption in the dark considerably, but the photosynthesis only slightly. The light synthesis of the enzyme was not affected, nor was the dark value influenced. At a concentration of 10-5 m there was a slight decrease in respiration and a greater decrease in the photosynthesis. The dark value of the GPD was not different from that of the controls whereas the synthesis in the light was markedly diminished. A concentration of 10-4 m completely blocks the respiration, the photosynthesis, and the light-induced GPD-synthesis; the dark value is decreased to about 50%. 7. It is concluded that the reversible synthesis of the NADP+-dependent GPD in light is dependent on chlorophyll and on an intact photosynthesis; the decisive reaction in this connection is the photoreaction II, the noncyclic electron flow.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Planta 70 (1966), S. 193-206 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Feinstruktur der Salzdrüsen in der Blattepidermis vonLimonium vulgare Mill. wurde elektronenmikroskopisch untersucht. Sie ähneln in ihrem Bau weitgehend den vonRuhland (1915) lichtmikroskopisch untersuchten Drüsen vonStatice Gmelini. Folgende Ergebnisse sind hervorzuheben: 1. Alle Drüsenzellen sind durch dichtes Plasma, groß Zellkerne, zahlreiche Mitochondrien und z. T. durch Wandprotuberanzen ausgezeichnet. 2. Die Durchlaßstellen in der kutinisierten Grenzkappe zwischen den äußeren Becherzellen und den Sammelzellen sind mit zahlreichen Plasmodesmen versehen. 3. Die Wandprotuberanzen in den Drüsenzellen werden häufig im Anschluß an Plasmodesmen gebildet und schließen nicht selten Plasmateile in sich ein. 4. In den inneren Becherzellen enthalten die Mitochondrien gelegentlich parakristalline Einschlüsse. 5. In den Drüsenzellen (außer den äußeren Becherzellen) werden häufig elektronendichte Bänder ausgebildet. 6. Die Grenzkappen unter den Sekretionsporen sitzen nicht direkt dem Plasma der Sekretionszellen, sondern deren Außenwand an.
    Notes: Summary The fine structure of the salt-glands in the leaf epidermis ofLimonium vulgare was investigated. The glands are very similar to those ofStatice Gmelini (Ruhland, 1915). The main results were as follows: 1. All gland cells have dense cytoplasma, large nuclei, numerous mitochondria and sometimes protuberances of the cell wall. 2. The passage spots in the cutinized “border cap” between the outer “cup cells” and the “collecting cells” show numerous plasmodesmata. 3. The cell wall protuberances in the gland cells often grow out in connection with plasmodesmata and not seldom include parts of the cytoplasm. 4. In the inner “cup cells” the mitochondria sometimes show paracrystalline inclusions. 5. In the gland cells (with the exception of the outer “cup cells”) electron-dense belts are often formed. 6. The “border caps” under the secretion-pores have no direct contact with the protoplast of the secretion cells but are attached to the outer cell wall.
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  • 10
    ISSN: 0931-1890
    Keywords: Key words Acyllipids ; Heartwood formation ; Phospholipids ; Robinia pseudoacacia L. ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The radial distribution of membrane and storage lipids was determined in the trunkwood of Robinia pseudoacacia L. The trees were felled in November at the time of heartwood formation and fluctuations in the amount and composition of phospholipids, free sterols, steryl esters, di- and triacylglycerols, and free fatty acids were investigated across the sapwood-heartwood boundary. The individual compounds were identified and quantified by thin layer chromatography, enzymatic and colorimetric assays, and by capillary gas chromatography. Phospholipids show a significant decrease towards the boundary area, and in the heartwood only trace amounts can be detected. The same pattern is observed for free sterols in the sapwood; in the heartwood, however, they reach maximum values with increasing depth of the trunk. Steryl esters exhibit a complementary behaviour by accumulating at the periphery of the heartwood. No concentration changes are found in the total amounts of diacylglycerols and free fatty acids. In contrast, the triacylglycerol concentration declines steadily across the trunk. With regard to qualitative composition, free fatty acids and the fatty acid moieties of the esterified constituents vary in their chain length from 14 to 24 carbon atoms and have up to three double bonds. A radial gradient in the ratio saturated/unsaturated fatty acids can be observed: except for the phospholipid fraction the relative amounts of unsaturated fatty acids increase in centripetal direction. Seven phospholipids were identified: phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, and phosphatidic acid, which constitutes the major proportion. In the sterol group, sitosterol is the most abundant component. The composition of the esterified sterols remains constant across the trunk cross-section, whereas the relative frequencies of individual free sterols change markedly.
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