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  • 1
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    A DNA-Based Biosensor Assay for the Kinetic Characterization of Ion-Dependent Aptamer Folding and Protein Binding (2019)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2019-08-08
    Description: Therapeutic and diagnostic nucleic acid aptamers are designed to bind tightly and specifically to their target. The combination of structural and kinetic analyses of aptamer interactions has gained increasing importance. Here, we present a fluorescence-based switchSENSE aptasensor for the detailed kinetic characterization of aptamer–analyte interaction and aptamer folding, employing the thrombin-binding aptamer (TBA) as a model system. Thrombin-binding aptamer folding into a G-quadruplex and its binding to thrombin strongly depend on the type and concentration of ions present in solution. We observed conformational changes induced by cations in real-time and determined the folding and unfolding kinetics of the aptamer. The aptamer’s affinity for K+ was found to be more than one order of magnitude higher than for other cations (K+ 〉 NH4+ 〉〉 Na+ 〉 Li+). The aptamer’s affinity to its protein target thrombin in the presence of different cations followed the same trend but differed by more than three orders of magnitude (KD = 0.15 nM to 250 nM). While the stability (kOFF) of the thrombin–TBA complex was similar in all conditions, the cation type strongly influenced the association rate (kON). These results demonstrated that protein–aptamer binding is intrinsically related to the correct aptamer fold and, hence, to the presence of stabilizing ions. Because fast binding kinetics with on-rates exceeding 108 M−1s−1 can be quantified, and folding-related phenomena can be directly resolved, switchSENSE is a useful analytical tool for in-depth characterization of aptamer–ion and aptamer–protein interactions.
    Electronic ISSN: 1420-3049
    Topics: Chemistry and Pharmacology
    Published by Molecular Diversity Preservation International
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    PAPER CURRENT
  • 2
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    Chapter 10 Conclusion (2022)
    Taylor & Francis | Routledge
    Details
    Publication Date: 2023-09-12
    Description: "This book provides a comparative overview of asylum seekers’ reception throughout Europe by adopting a theoretical framework based on an analytical approach to the notion of multilevel governance. It challenges the tendency of the multilevel governance literature to overlook political controversies and conflicts and questions the assumption that it represents the best policymaking arrangement for promoting policy convergence. In doing so, it explores the functioning of the reception component of the Common European Asylum System in centralised states and federal/regional states and analyses its implementation at both national and local levels. The book reveals the heterogeneous development of reception policies not only across Member States but also within each country where solutions adopted at the local level generally diverge substantially. Furthermore, the overall centralization of policymaking on reception regardless the institutional structure, seems to leave little room for MLG arrangements tailored to specific localities and triggers tensions between central governments and local authorities. This book will be of key interest to scholars and students of migration and asylum studies, immigration, (multilevel) global governance and more broadly to comparative politics, European studies/politics, and public policy."
    Keywords: asylum; asylum seekers; Common European Asylum System; Europe; multilevel governance; policymaking ; bic Book Industry Communication::J Society & social sciences::JP Politics & government ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPQ Central government::JPQB Central government policies ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPS International relations ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general::JFF Social issues & processes::JFFS Globalization ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPV Political control & freedoms::JPVH Human rights
    Language: English
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    E-BOOK
  • 3
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    Chapter 5 The variance in multilevel governance of asylum seekers’ reception in Italy (2022)
    Taylor & Francis | Routledge
    Details
    Publication Date: 2024-03-29
    Description: "This book provides a comparative overview of asylum seekers’ reception throughout Europe by adopting a theoretical framework based on an analytical approach to the notion of multilevel governance. It challenges the tendency of the multilevel governance literature to overlook political controversies and conflicts and questions the assumption that it represents the best policymaking arrangement for promoting policy convergence. In doing so, it explores the functioning of the reception component of the Common European Asylum System in centralised states and federal/regional states and analyses its implementation at both national and local levels. The book reveals the heterogeneous development of reception policies not only across Member States but also within each country where solutions adopted at the local level generally diverge substantially. Furthermore, the overall centralization of policymaking on reception regardless the institutional structure, seems to leave little room for MLG arrangements tailored to specific localities and triggers tensions between central governments and local authorities. This book will be of key interest to scholars and students of migration and asylum studies, immigration, (multilevel) global governance and more broadly to comparative politics, European studies/politics, and public policy."
    Keywords: asylum; asylum seekers; Common European Asylum System; Europe; multilevel governance; policymaking ; bic Book Industry Communication::J Society & social sciences::JP Politics & government ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPQ Central government::JPQB Central government policies ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPS International relations ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general::JFF Social issues & processes::JFFS Globalization ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPV Political control & freedoms::JPVH Human rights ; thema EDItEUR::J Society and Social Sciences::JP Politics and government ; thema EDItEUR::J Society and Social Sciences::JP Politics and government::JPQ Central / national / federal government::JPQB Central / national / federal government policies ; thema EDItEUR::J Society and Social Sciences::JP Politics and government::JPS International relations ; thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GT Interdisciplinary studies::GTQ Globalization ; thema EDItEUR::J Society and Social Sciences::JP Politics and government::JPV Political control and freedoms::JPVH Human rights, civil rights
    Language: English
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    E-BOOK
  • 4
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    Chapter 3 Reforms of asylum seekers’ reception during the 2010s (2022)
    Taylor & Francis | Routledge
    Details
    Publication Date: 2023-09-12
    Description: "This book provides a comparative overview of asylum seekers’ reception throughout Europe by adopting a theoretical framework based on an analytical approach to the notion of multilevel governance. It challenges the tendency of the multilevel governance literature to overlook political controversies and conflicts and questions the assumption that it represents the best policymaking arrangement for promoting policy convergence. In doing so, it explores the functioning of the reception component of the Common European Asylum System in centralised states and federal/regional states and analyses its implementation at both national and local levels. The book reveals the heterogeneous development of reception policies not only across Member States but also within each country where solutions adopted at the local level generally diverge substantially. Furthermore, the overall centralization of policymaking on reception regardless the institutional structure, seems to leave little room for MLG arrangements tailored to specific localities and triggers tensions between central governments and local authorities. This book will be of key interest to scholars and students of migration and asylum studies, immigration, (multilevel) global governance and more broadly to comparative politics, European studies/politics, and public policy."
    Keywords: asylum; asylum seekers; Common European Asylum System; Europe; multilevel governance; policymaking ; bic Book Industry Communication::J Society & social sciences::JP Politics & government ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPQ Central government::JPQB Central government policies ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPS International relations ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general::JFF Social issues & processes::JFFS Globalization ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPV Political control & freedoms::JPVH Human rights
    Language: English
    Format: image/jpeg
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    E-BOOK
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