Publication Date:
2016-03-05
Description:
Translation of messenger RNAs lacking a stop codon results in the addition of a carboxy-terminal poly-lysine tract to the nascent polypeptide, causing ribosome stalling. Non-stop proteins and other stalled nascent chains are recognized by the ribosome quality control (RQC) machinery and targeted for proteasomal degradation. Failure of this process leads to neurodegeneration by unknown mechanisms. Here we show that deletion of the E3 ubiquitin ligase Ltn1p in yeast, a key RQC component, causes stalled proteins to form detergent-resistant aggregates and inclusions. Aggregation is dependent on a C-terminal alanine/threonine tail that is added to stalled polypeptides by the RQC component, Rqc2p. Formation of inclusions additionally requires the poly-lysine tract present in non-stop proteins. The aggregates sequester multiple cytosolic chaperones and thereby interfere with general protein quality control pathways. These findings can explain the proteotoxicity of ribosome-stalled polypeptides and demonstrate the essential role of the RQC in maintaining proteostasis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Choe, Young-Jun -- Park, Sae-Hun -- Hassemer, Timm -- Korner, Roman -- Vincenz-Donnelly, Lisa -- Hayer-Hartl, Manajit -- Hartl, F Ulrich -- England -- Nature. 2016 Mar 10;531(7593):191-5. doi: 10.1038/nature16973. Epub 2016 Feb 29.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26934223" target="_blank"〉PubMed〈/a〉
Keywords:
Alanine/metabolism
;
Codon, Terminator/genetics
;
Inclusion Bodies/chemistry/*metabolism
;
Molecular Chaperones/metabolism
;
Polylysine/metabolism
;
Proteasome Endopeptidase Complex/metabolism
;
*Protein Aggregates
;
Protein Aggregation, Pathological
;
Protein Biosynthesis
;
Proteolysis
;
RNA-Binding Proteins/*metabolism
;
Ribosomes/*metabolism
;
Saccharomyces cerevisiae/enzymology/*metabolism
;
Saccharomyces cerevisiae Proteins/genetics/*metabolism
;
*Stress, Physiological
;
Threonine/metabolism
;
Ubiquitin-Protein Ligases/*deficiency/genetics/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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