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  • 1
    Electronic Resource
    Electronic Resource
    Construction of transposon Tn3phoA: its application in defining the membrane topology of the Agrobacterium tumefaciens DNA transfer proteins (1998)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 27 (1998), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Protein fusion with the Escherichia coli alkaline phosphatase is used extensively for the analysis of the topology of membrane proteins. To study the topology of the Agrobacterium T-DNA transfer proteins, we constructed a transposon, Tn3phoA. The transposon mobilizes into plasmids at a high frequency, is stable after transposition, can produce phoA translational fusions and can be used for the analysis of protein topology directly in the bacterium of interest. For studies on the DNA transfer proteins, an Agrobacterium strain deficient in phoA under our experimental conditions was constructed by chemical mutagenesis. A plasmid containing virB and virD4 was used as a target for mutagenesis. Twenty-eight unique phoA-positive clones that mapped to eight virB genes were isolated. Multiple insertions throughout VirB1, VirB5, VirB7, VirB9 and VirB10 indicated that these proteins primarily face the periplasm. Insertions in VirB2, VirB6 and VirB8 allowed the identification of their periplasmic domains. No insertions were found in VirB3, VirB4 and VirB11. These proteins either lack or have a short periplasmic domain. No insertions mapped to VirD4 either. To study VirD4 topology, targeted phoA fusions and random lacZ fusions were constructed. Analysis of the fusion proteins indicated that VirD4 contains a single periplasmic domain near the N-terminus, and most of the protein lies in the cytoplasm. A hypothetical model for the T-DNA transport pore is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1998.00688.x
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  • 2
    Electronic Resource
    Electronic Resource
    Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore (2000)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 36 (2000), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Agrobacterium tumefaciens transforms plants by transferring DNA to the plant cell nucleus. The VirB membrane proteins are postulated to form a pore for the transport of the DNA across the bacterial membranes. Immunofluorescence and immunoelectron microscopy were used to study the transport pore complex. Three likely components of the transport pore, VirB8, VirB9 and VirB10, localized primarily to the inner membrane, outer membrane and periplasm respectively. A significant amount of VirB10 was also found associated with the outer membrane. When expressed alone VirB9 and VirB10 were randomly distributed along the cell membrane. Subcellular location of both proteins changed dramatically in the presence of the other VirB proteins. Both proteins localized to fewer sites and most of the gold particles representing protein molecules were found in clusters suggesting that the two proteins are in a protein complex. VirB8, on the other hand, localized to clusters even in the absence of the other VirB proteins. To investigate the role of VirB8 in the formation of VirB9 and VirB10 protein complexes, we studied the effect of deletion of virB8 on the subcellular location of VirB9 and VirB10. In a virB8 deletion mutant both proteins were distributed randomly on the cell membrane indicating that VirB8 is essential for complex assembly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2000.01876.x
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  • 3
    Electronic Resource
    Electronic Resource
    4-[(3-Pyridylamino)methyl]phenol (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. o411-o413 
    Details
    ISSN: 1600-5368
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The title compound, C12H12N2O, crystallizes in the non-centrosymmetric P212121 space group giving crystals showing a second harmonic generation (SHG) property. The crystal structure consists of discrete molecules and forms a three-dimensional network through intermolecular hydrogen bonding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S1600536801005037
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  • 4
    Electronic Resource
    Electronic Resource
    New Fatigue Model for Service Life Assessment of Last Stage Blade in Steam TurbineBased on Liquid Corrosion and Dynamic Stress (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 348-349 (Sept. 2007), p. 613-616 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Forced vibration and liquid corrosion are important reasons for last stage blade failure insteam turbine. In order to improve reliability of blades in wet steam stages, the dynamic stress andthe liquid-solid impact stress of blade were analyzed firstly, then Neuber’s rule, rainflow countingmethod and improved cumulative damage rule were introduced to develop a new model to evaluateservice life of last stage blade. Many factors such as manufacturing quality, metal quality andoperation status were considered, so it is more reasonable for service life assessment of last stageblade. At last, the service life of a last stage blade of 680mm was analyzed and the resultapproximates the practical service life. It is shown the new fatigue model can give some significantdata and is usable for engineering application
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/55/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.348-349.613.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    Study on Service Life of Last Stage Blade with Damped Structure in Large Power Steam Turbine (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 44-46 (June 2008), p. 803-808 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Blade which transfers thermal energy of steam into power, is a basic component in steamturbine. The reliability of blade is heavily influenced by the operating environment. The rotating bladeexperiences large inertial load and the wake of nozzle flow impose large variations of aerodynamicload on blade, in addition, the last stage blade is also affected by corrosion, so accidents of bladehappen from time to time. Preventing blade failure has become one of the major objectives of turbinedesign and in-service maintenance. It is said that the reason for most of blade failure is fatiguefracture. In this study, a synthetical numerical model has been developed to evaluate service life ofblade. At first, a numerical model to analyze the excitation force, dynamic frequency and dynamicstress of steam turbine blade has been developed, based on the results of dynamic stress analysis, amodel to evaluate the service life of turbine blade has been developed. Many factors such asmanufacturing technology of blade and erosion operating environment are considered to get moreaccurate results for service life of blade. At last, a last stage blade group of a large power steam turbineis analyzed in detail. It is shown clearly that the numerical model can give some rational quantitativeresults, and it is suitable for its engineering application to the improvement of the blade reliability
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/40/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.44-46.803.pdf
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  • 6
    Electronic Resource
    Electronic Resource
    Analytical Research on FEM and Dynamic Stress Test of Residue Plastic Film Suspension Recycling Machine (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 33-37 (Mar. 2008), p. 1389-1392 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper carried on mechanics analysis and the research to the Curved Rake Tine Frameand Headstock of film recycling machine which could cut cotton stalk and recycle the used filmsimultaneously. This machine was developed by the Institute of Agriculture Machine XingjiangAcademy of Agricultural Sciences. This paper used servile methods to identify the structuraldeficiencies such as Visualization Virtual Design, Finite Element Method and Testing Method. Thesedata provided a theoretical basis for the design of the Curved Rake Tine Frame and Headstock
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/40/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.33-37.1389.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    A Study on Co and Cu Oxides as Sintering Aids for Sm〈sub〉0.2〈/sub〉Ce〈sub〉0.8〈/sub〉O〈sub〉1.9〈/sub〉 Electrolyte (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 539-543 (Mar. 2007), p. 1391-1396 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this study, an addition of Co and Cu oxides to Sm0.2Ce0.8O1.9 (SDC) was studied toimprove the SDC sinterability. It has been found that both Co and Cu oxide are very effective assintering aids, and the SDC sintering temperature can be reduced from 1400°C without aids tobelow 1000°C with only 1at.% of either Cu or Co. As compared to the pure SDC, a slight decreaseof ionic conductivity was observed in SDC with Cu sintering aid. There is no obvious effect onelectrochemical property of SDC with Co sintering aid under 2.5at.%
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/15/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.1391.pdf
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  • 8
    Electronic Resource
    Electronic Resource
    Mechanical Strength and Interface Adhesion of a Solid Oxide Fuel Cell with Doped Ceria Electrolyte (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 539-543 (Mar. 2007), p. 1421-1426 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An SOFC must have sufficient mechanical strength and interface adhesion to ensure itcan be handled without breakage during fabrication and assembly, and has desired performance andreliability. Methods for measuring mechanical properties and interface adhesion of an SOFC havebeen developed and measurements made on a cermet-supported SOFC with a SDC electrolyte. TheSOFC evaluated had a porous NiO-YSZ substrate, a porous NiO-SDC anode and a dense SDCelectrolyte fabricated using tape-casting, screen-printing and co-firing techniques. The flexuralstrength and interface adhesion of the substrate, the anode and the electrolyte, along with theirYoung’s modulus, hardness and residual stress, were quantitatively measured. The results of themeasurements indicate that the NiO-YSZ supported, SDC electrolyte SOFC has adequatemechanical strength and sufficient interface adhesion
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/15/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.1421.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    Influence of Compound Additive upon the Freeze-Thaw Resistance of Concrete (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 302-303 (Jan. 2006), p. 184-190 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/50/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.302-303.184.pdf
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  • 10
    Electronic Resource
    Electronic Resource
    Synthetical Numerical Model for Evaluating Blade Fatigue Life of Liquid Corrosion in Steam Turbine (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 324-325 (Nov. 2006), p. 165-168 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Liquid corrosion of steam turbine blade has become an important reason for blade failurein supercritical parameter power plant and nuclear power plant. In order to improve the reliability ofblades in wet steam stages of steam turbine and prevent blade failure from liquid corrosion, anonlinear coupling wave model has been developed to investigate the high speed impact betweenball liquid droplet and elastic solid, according to a mass of numerical results of impact betweenliquid droplet and elastic solid made by 1Cr13 which is a typical material for steam turbine blade,the relational expression between dimensionless equivalent peak stress and impact speed machnumber is obtained. Then suitable equations to evaluate the fatigue life of crack initiation andnonlinear cumulative damage rule are adopted to develop numerical model for liquid corrosionfatigue life of blade, and the liquid corrosion fatigue life of a wet steam blade is analyzed in detail.It is shown that the nonlinear coupling wave model for impact and the analysis model of liquidcorrosion fatigue life for blade can give some quantitative results, it may be helpful to evaluateliquid corrosion fatigue life of blade and prevent blade from liquid corrosion during blade design
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.324-325.165.pdf
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