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  • 1
    Electronic Resource
    Electronic Resource
    Distinct Spatio-temporal Distributions of the NMDA Receptor Channel Subunit mRNAs in the Brain (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 707 (1993), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1993.tb38099.x
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of TiO〈sub〉2〈/sub〉 Thin Films by Electrophoresis Deposition Method in Highly Dispersed Colloidal Solution (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 388 (Sept. 2008), p. 115-118 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The purpose of this study is to prepare high-quality TiO2 thin films suitable for dye-sensitized solarcells using electrophoresis method. A high-quality TiO2 thin film without crack could be depositedusing highly dispersed colloidal solution of TiO2 fine particles. Highly dispersed colloidal solutionwas prepared using mechanical milling (MM). The two electrodes of electrophoresis were set parallelto liquid level. Optimum preparing condition to disperse TiO2 fine particles using MM couldbe found. Under this condition, homogeneous TiO2 thin film was deposited on ITO glass. Theopen-electromotive force and short-circuit current of dye-sensitized solar cell showed excellent reproducibility
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.388.115.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Variations in immunocytochemical localization of cathepsin B and thyroxine in follicular cells of the rat thyroid gland and plasma TSH concentrations over 24 hours (1989)
    Springer
    Cell & tissue research 256 (1989), S. 355-360 
    Details
    ISSN: 1432-0878
    Keywords: Thyroid gland ; Cathepsin B ; Lysosomes ; Immunocytochemistry ; Diurnal rhythm ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical localization of cathepsin B and thyroxine (T4) in follicular cells of the rat thyroid gland and plasma concentrations of thyroid stimulating hormone (TSH) were examined at six evenly spaced times over 24 h. By light- and electron microscopy, immunodeposits for cathepsin B were localized in cytoplasmic granules of various sizes, whereas those for T4 were detected mainly in larger granules of the cells and in the colloid lumen. The size and location of cytoplasmic granules showing immunoreactivity for cathepsin B and T4 in the cells varied over 24 h, corresponding to a change in plasma TSH concentrations. These immunopositive large granules appeared in the apical cytoplasm at 12.00 h, when the level of TSH was highest. At 20.00 h when the level of TSH was lowest, T4-positive granules almost disappeared, and cathepsin B-positive small granules were abundantly seen in the basal region. From 00.00 h to 08.00 h, these positive granules changed in the same manner as those seen from 12.00 h to 20.00 h, associated with an increase in plasma TSH levels. These results suggest that newly formed colloid droplets migrate from the apical to the basal regions. Cathepsin B may play a role not only in the degradation of thyroglobulin but in the maturation of thyroid hormones during the migration of the granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218892
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  • 4
    Electronic Resource
    Electronic Resource
    A morphometric study of developing pancreatic acinar cells of rats during prenatal life (1984)
    Springer
    Cell & tissue research 237 (1984), S. 117-122 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas, exocrine (rat) ; Fine structure ; Membrane dynamics ; Development (ontogenetic) ; Morphometry ; Zymogen granules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pancreatic acinar cells of rat embryos obtained at 15, 17, 19 and 21 days of gestation have been examined using fine-structural and morphometric techniques. Morphometric analysis demonstrates significant variations in the average volume of the cell, nucleus and cytoplasm, and the volume, surface and numerical densities of various cytoplasmic organelles during fetal life. In particular, the volume and surface densities of rER exhibit maximal values at 19 days of gestation, suggesting that secretory proteins are produced most actively at this time. Further-more, membrane continuity between the nuclear envelope and rER is frequently discernible in acinar cells, indicating that at this stage the rER is mainly derived from the nuclear envelope. Zymogen granules first appear at 17 days of gesstation. By 21 days, they occupy the greater part of the cytoplasm of the acinar cells, no polarity being seen in their distribution pattern. No direct evidence for the secretion of zymogen granules has been observed during fetal life. It therefore appears that membrane transport involved with intracellular movement of newly synthesized proteins from rER via the Golgi complex to zymogen granules occurs in one direction and lacks regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229206
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  • 5
    Electronic Resource
    Electronic Resource
    A morphometric study of the 24-hour variations in subcellular structures of rat pancreatic acinar cells during the periweaning period (1984)
    Springer
    Cell & tissue research 237 (1984), S. 131-138 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas, exocrine (rat) ; Circadian rhythm ; Morphometry ; Periweaning period
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pancreatic acinar cells of rats obtained before (21 days of age) and after (31 and 42 days) weaning were morphometrically examined at six evenly spaced times during 24 h. Morphometric analysis demonstrated clear-cut variations in the average volume of the cell and nucleus, the incidence of binucleate acinar cells, and the volume and numerical densities of various cytoplasmic organelles during 24 h, at each stage. The daily mean values and variation of these parameters at 21 days clearly differed from those at 31 and 42 days. In particular, changes in the volume densities of rER and zymogen granules were bimodal at 21 days, whereas they were unimodal at 31 and 42 days. Moreoever, some parameters also differed between 31 and 42 days, the main difference being an 8 h time shift in the variation curves of the volume densities of rER and zymogen granules. These findings indicate that morphometric analysis of the subcellular structures of pancreatic acinar cells during 24 h can be used to determine their developmental stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229208
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  • 6
    Electronic Resource
    Electronic Resource
    Morphometric and fine-structural studies of rat pancreatic acinar cells during early postnatal life (1984)
    Springer
    Cell & tissue research 237 (1984), S. 123-129 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas, exocrine (rat) ; Ultrastructure ; Morphometry ; Development, ontogenetic ; Zymogen granules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pancreatic acinar cells of rats obtained at 1,2, 3, 5, 7 and 14 days of age were examined using fine structural and morphometric techniques. From 5 days of age onwards, the acinar cells were analysed twice per day, at 20.00 h and 08.00 h. The present study demonstrates changes in the average volume of the cell, nucleus and cytoplasm, and volume densities of various cytoplasmic organelles during the first two weeks after birth. During early postnatal life, the volume density of rER increases, whereas that of zymogen granules decreases. From 5 days of age onwards, the volume densities of these two organelles differ significantly at 20.00 h and 08.00 h. During the first 2–3 days after birth, inclusion body-like structures appear in the cytoplasm of acinar cells; they contain aggregated zymogen granules and, sometimes, amorphous structures or cytoplasmic organelles. These structures also occur in interstitial cells and cells located in the intercalated region between acinar and ductal epithelial cells. Serum level of α-amylase activity is high at birth, compared with other stages during the first three weeks. Degenerating acinar cells and cell debris can be seen in the acinar and ductal lumina during these stages, a feature suggesting holocrine secretion. Cellular polarity appears to be incomplete during the first two or three days after birth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229207
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  • 7
    Electronic Resource
    Electronic Resource
    Twenty four hour variations in subcellular structures of rat pancreatic islet B-, A- and D-cells, and of portal plasma glucose and insulin levels (1988)
    Springer
    Cell & tissue research 253 (1988), S. 337-345 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas, endocrine ; Morphometry ; Plasma glucose ; Plasma insulin ; Circadian variation ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Variations in stomach weights, plasma glucose and insulin levels in the portal vein, and in subcellular structures of rat pancreatic islet B-, A- and D-cells were examined over 24 h. The variation in stomach weights paralleled plasma glucose levels, indicating that the levels may be influenced by intestinal glucose absorption. Plasma insulin levels increased from the late dark to the early light periods, whereas they decreased from the late light to the early dark periods. The variation in plasma insulin levels was in the opposite sense to that in the relative numbers of B-cell granules. The decrease in the relative numbers of A-cell granules occurred between the late dark and early light periods. The relative numbers of D-cell granules decreased before and after the decreases in B- and A-cell granules. The variation in D-cell granules appears to correspond to the inhibitory effect of somatostatin. The relative amounts of rough endoplasmic reticulum (rER) in each cell varied in a reciprocal manner to those of B-cell granules. Moreover, the variation in plasma insulin levels coincided with variations in rER of hepatocytes and pancreatic acinar cells. The changes in rER of each cell may correlate with the trophic effect of insulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222290
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  • 8
    Electronic Resource
    Electronic Resource
    Involvement of umuDC ST genes in nitropyrene-induced -CG frameshift mutagenesis at the repetitive CG sequence in the hisD3052 allele of Salmonella typhimurium (1995)
    Springer
    Molecular genetics and genomics 247 (1995), S. 7-16 
    Details
    ISSN: 1617-4623
    Keywords: umuDC ST ; Frameshift mutagenesis Nitropyrenes ; Repetitive CG sequence ; Salmonella typhimurium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of the umuDC operon is required for UV and most chemical mutagenesis in Escherichia coli. The closely related species Salmonella typhimurium has two sets of umuDC-like operons, umuDC ST on the chromosome and samAB on a 60-MDa cryptic plasmid. The roles of theumuDC-like operons in chemically induced frameshift mutagenesis of the hisD3052 allele of S. typhimurium were investigated. Introduction of a pBR322-derived plasmid carrying umuDCST increased the rate of reversion of hisD3052, following treatment with 1-nitropyrene (1-NP) or 1,8-dinitropyrene (1,-8DNP) tenfold and fivefold, respectively, whereas it did not substantially increase the rate of reversion induced by other frameshift mutagens, i.e. 2-nitrofluorene (2NF) and 2-amino- 3-methyldipyrido[1,2-a:3 ′,2′-d]imi-dazole (Glu-P-1). Introduction of a pBR322-derived plasmid carrying samAB did not increase the incidence of reversion of hisD3052 observed with any of the mutagens examined. Deletion of umuDC STSubstantially lowered the reversion rate induced by l-NP or 1,8-DNP, but it did not affect reversion induced by 2-NF, Glu-P-1 or N-hydroxyacetylaminofluorene (N-OH-AAF). Deletion of samAB had little impact on reversion incidence induced by any of the five frameshift mutagens. DNA amplification using the polymerase chain reaction technique followed by restriction enzyme analysis using BssHII, suggested that the mutations induced by the five frameshift mutagens were all CG deletions at the CGCGCGCG sequence in hisD3052. These results suggest that umuDCST, but not samAB, is involved in the -2 frameshift mutagenesis induced by l-NP and 1,8-DNP at the repetitive CG sequence, whereas neither operon participates in induction of the same type of mutations by 2-NF, Glu-P-1 or N-OH-AAF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425816
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  • 9
    Electronic Resource
    Electronic Resource
    Electrophoretic heterogeneity and age-related change of serine proteinase fromAspergillus sojae (1986)
    Springer
    Current microbiology 13 (1986), S. 231-235 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Five forms of serine proteinase (EC 3.4.21.14) have been purified fromAspergillus sojae. This paper reports heterogeneity of electrophoretic mobilities, isoelectric points, and kinetic parameters of multiple forms of serine proteinase fromAspergillus sojae, including agerelated changes in thek cat /K m of electrophoretic species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01568952
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  • 10
    Electronic Resource
    Electronic Resource
    A linkage study with DNA markers (D4S95, D4S115, and D4S111) in Japanese Huntington disease families (1993)
    Springer
    Journal of human genetics 38 (1993), S. 193-201 
    Details
    ISSN: 1435-232X
    Keywords: Huntington disease ; linkage analysis ; D4S95 ; D4S115 ; D4S111
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Attempts to isolate the Huntington disease (HD) gene based on its position have been frustrated by apparently contradictory recombination events in HD pedigrees that have predicted two non-over-lapping candidate regions: 100 kb at the telomere of the short arm of chromosome 4, and a 2.2 Mb region located internally at 4p16.3. The proximal location is also supported by the detection of a linkage disequilibrium between HD and some restriction fragment length polymorphisms (RF-LPs) at the D4S95, D4S98, and D4S127 loci. In the present study, a proximal marker D4S95 showed tight linkage to the disease locus in Japanese pedigrees (Zmax=3.31,θ max=0.00), while distal markers D4S115 and D4S111 did not. Particularly, a two point linkage analysis between D4S111 and HD yielded a lod score −2.01 for θ=0.015. This result leads to the exclusion, as a possible region of localization of the HD gene, of more than 3 cM of the genome around D4S111 locus. At the same time our results favor aforementioned proximal location as a candidate location for the HD gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01883710
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