ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
In this study, three kinds of photo-reactive polymers, namely positively chargedazidophenyl-derived polyallylamine (AzPhPAAm), negatively charged azidophenyl-derivedpoly(acrylic acid) (AzPhPAAc) and neutral azidophenyl-derived poly(ethylene glycol) (AzPhPEG),were synthesized by introduction of photo-reactive group (-N3) into polyallylamine, poly(acrylicacid) and poly(ethylene glycol), respectively. The structure of the azidophenyl-derived polymerswas confirmed by 1H-NMR measurement. All of the photo-reactive polymers can be pattern-graftedon the surface of cells culture plate, which was confirmed by the optical microscopy observation. Thecontact angle decreased after surface modification by the photo-reactive polymers. Humanmesenchymal stem cells (MSC) cultured on the modified surfaces showed different morphology.The cells adhered and spread more on the PAAm- and PAAc-grafted surfaces than on thePEG-grafted surface. The pellets formed on PAAm- and PEG-grafted surfaces in condrogenicdifferentiation medium were positively stained by safranin O/ fast green. But the pellet formed onPAAc-grafted surface did not show obviously positive staining for safranin O/ fast green. Theseresults indicate that the PAAm- and PEG-grafted surfaces promoted chondrogenic differentiation ofMSC
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1189.pdf
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