ISSN:
1573-6822
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Previous studies showed that Cd++ inhibits EGF-induced DNA synthesis that not EGF-induced myc mRNA accumulation or amino acid incorporation into protein in serum-starved NRK-49F cells. In this study, flow cytometry was used to analyze the DNA and protein content of individual cells stimulated with Cd++ and/or epidermal growth factor (EGF). myc oncogene expression in these cells was also measured. It was found that, in both parental NRK-49F cells and in a clonal subpopulation, N1, Cd++ induces an hypertrophic response. In parental NRK-49F cells, however, lower doses of Cd++ (0.5 μM) induced more pronounced hypertrophic responses than did higher doses (4 μM); whereas in N1 cells, the Cd++-induced hypertrophic response shows a pattern of increasing response with doses of Cd++ from 0.5 to 4 μM. myc mRNA accumulation measured 2 hours after stimulation correlated with the hypertrophic responses in both NRK-49F cells and in N1 cells. The results show that Cd++-induced hypertrophy in NRK-49F cells is associated with increased myc oncogene mRNA accumulation, indicating that cell proliferation and cell hypertrophy may in part share common activation pathways.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00124074
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