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  • 1
    Electronic Resource
    Electronic Resource
    Kennedy's deed (1975)
    [s.l.] : Nature Publishing Group
    Nature 258 (1975), S. 475-475 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR,-You include a comment (November 6, p5) entitled "Kennedy's good deed" regarding the U.S. Senate vote to ban the use of diethylstiboestrol as cattle feed supplement. A number of controversial matters are introduced into this short article, but I wish to protest the attribution of purely ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/258475c0
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibition by Puromycin of Incorporation of Tritiated Uridine into Nucleolar and Cytoplasmic Ribonucleic Acids (1966)
    [s.l.] : Nature Publishing Group
    Nature 212 (1966), S. 194-196 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] HeLa cells were grown as monolayers in multiple cultures on glass coverslips bound by metal rings6. The cells were incubated at 37 C in a humidified atmosphere of 5 per cent carbon dioxide in air. Eagle's minimal essential medium was supplemented with 8 per cent horse serum, 2 rnM glutamine and 200 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/212194b0
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  • 3
    Electronic Resource
    Electronic Resource
    Instability of Puromycin (1966)
    [s.l.] : Nature Publishing Group
    Nature 212 (1966), S. 196-197 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The parameter of puromycin action investigated in one laboratory (Jefferson Medical College) was its inhibitory effect on the division of cultured mammalian cells. HeLa S3 cells were grown in multiple replicate ring cultures as described previously8. Varying concentrations of two preparations of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/212196a0
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  • 4
    Electronic Resource
    Electronic Resource
    Nucleolus-like Inclusions in the Cytoplasm of HeLa Cells treated with Puromycin (1964)
    [s.l.] : Nature Publishing Group
    Nature 203 (1964), S. 883-884 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Inclusions were produced in HeLa cells grown in monolayer culture under conditions described by Wildy et al4. Unless otherwise stated in the protocol, 100 mg of puromycin dihydrochloride (Nutritional Biochemical Corp., Cleveland, Ohio) was present in each 1 ml. of the culture medium and the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/203883a0
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  • 5
    Electronic Resource
    Electronic Resource
    Cytochemical Investigations of Deoxyribonucleic Acid-mediated Control of Ribonucleoprotein Metabolism (1964)
    [s.l.] : Nature Publishing Group
    Nature 203 (1964), S. 1384-1384 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cells were stained by the toluidine blue-molybdate method4. Nuclear and cytoplasmic fractions were prepared by the citric acid procedure5. Nucleic acids were estimated by a modified Schmidt-Tannhauser procedure6, Burton?s modification of the Dische reaction7 and the orcinol reaction8. Protein was ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2031384a0
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  • 6
    Electronic Resource
    Electronic Resource
    Resistance of mitochondrial DNA to degradation characterizes the apoptotic but not the necrotic mode of human leukemia cell death (1993)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 52 (1993), S. 352-361 
    Details
    ISSN: 0730-2312
    Keywords: necrosis ; cell death ; cell membrane integrity ; lysosomal enzymes ; apoptosis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Cell death can occur by two basically different processes. The original term, necrosis, is now reserved for the generally destructive series of events which include the release of lysosomal enzymes and loss of cell membrane integrity. In contrast, mild treatment with cell damaging agents, or withdrawal of growth factors, may result in a characteristic form of degradation of cellular DNA which is associated with cell death that has morphology known as apoptosis. In this study human leukemia cells were exposed to agents or conditions previously reported to cause necrosis or apoptosis, monitored by detection of DNA “ladders,” and the integrity of cellular DNA was determined on Southern blots. Nuclear DNA was distinguished from mitochondrial DNA by use of probes specific for nuclear genes or for mitochondrial DNA. When HL60, K562, MOLT4, or U937 cells were exposed to conditions which resulted in necrosis, mitochondrial DNA was damaged at approximately the same rate as nuclear DNA, but in apoptosis mtDNA was not degraded. Thus, the ratio of the relative (to untreated cells) abundance of mitochondrial DNA measured by a probe for 16S mitochondrial ribosomal RNA on Southern blots, to the relative abundance of DNA of any nuclear gene, was 1 or less in necrosis, but rose to values greater than 2 in apoptosis. It is concluded that the comparison of the degree of fragmentation of mitochondrial and nuclear DNA provides a quantitative way of distinguishing necrosis from apoptosis.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240520311
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  • 7
    Electronic Resource
    Electronic Resource
    Vitamin D analog 25-(OH)-16,23E-diene-26,27-hexafluoro-vitamin D3 induces differentiation of HL60 cells with minimal effects on cellular calcium homeostasis (1996)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 63 (1996), S. 500-512 
    Details
    ISSN: 0730-2312
    Keywords: vitamin D3 ; differentiation ; intracellular calcium ; store-dependent calcium influx ; cell cycle blocks ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Numerous vitamin D3 analogs (VDAs) can inhibit the proliferation of cells from several types of human malignancies. The physiologically active form of vitamin D3, 1,25-dihydroxyvitamin D3(1,25D3), is formed by successive hydroxylations of cholecalciferol at the 25 and 1α positions. In this study we examined the effects of the absence of the 1α(OH) group, introduction of a double bond in position 16, and further modifications at the 23, 26, and 27 positions in the side chain on the potency of the VDAs. The parameters studied were the rapidity of the induction of monocytic differentiation, the cell cycle traverse, and the effects of VDAs on intracellular calcium homeostasis in HL60 cells. The results show that (1) 1,25D3 derivatives which lack the 1α(OH) group have little differentiation-inducing activity, (2) hexafluorination (6F) of the terminal methyl groups in the side chain partially restores the activity of 1α-desoxy compounds and potentiates the activity of 1α hydroxylated compounds, and (3) 25-(OH)-16,23E-diene-26,27-hexafluoro-vitamin D3 (Ro25-9887) alone among the twelve compounds tested induces differentiation with only minimal changes in the basal levels of intracellular calcium and store-dependent calcium influx in HL60 cells. Addition of 1α(OH) group to this compound increases its differentiation-inducing activity but also elevates basal calcium level. The results suggest that altered calcium homeostasis is not an obligatory component of HL60 leukemia cell differentiation, and that Ro25-9887 and related VDAs may be suitable for testing as components of anti-leukemic therapy. © 1996 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    Downregulation of telomerase activity in HL60 cells by differentiating agents is accompanied by increased expression of telomerase-associated protein (1997)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 67 (1997), S. 13-23 
    Details
    ISSN: 0730-2312
    Keywords: telomerase ; TP1 ; HL60 cells ; leukemia ; differentiation therapy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Telomerase activity provides a mechanism for the unlimited division potential of neoplastic cells. Induced differentiation of these cells was found to be associated with repression of telomerase activity irrespective of the inducing agent. We have employed a series of sublines of human promyelocytic leukemia line HL60 with differing degrees of resistance to differentiation to determine how tightly the expression of the differentiated phenotype is coupled to the downregulation of telomerase activity and to the expression of the recently identified telomerase-associated protein 1 (TP1). As expected, in the 1,25D3-dihydroxyvitamin D3 (1,25D3)-resistant subclones (20A-100A cells), telomerase activity was not significantly downregulated by 1,25D3 and, in most cases, by all-trans retinoic acid (atRA), to which these cells were cross-resistant, but telomerase activity was repressed by dimethylsulfoxide (DMSO) and phorbol-12-myristate-13-acetate (TPA), to which the sublines were in general sensitive. However, there were exceptions; in some instances telomerase activity was repressed in the absence of the expression of markers of differentiation. Also, there was an inverse relationship between telomerase activity and the cellular levels of TP1 transcripts. We conclude that in HL60 cells downregulation of telomerase is loosely associated with upregulation of differentiation markers and with other cellular changes which include an upregulation of TP1. J. Cell. Biochem. 67:13-23, 1997. © 1997 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Inhibition of DNA synthesis by an inducer of differentiation of leukemic cells, 1 alpha, 25 dihydroxy vitamin D3, precedes down regulation of the c-myc gene (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 128 (1986), S. 171-179 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Transient monocytic differntiation was induced in cultures of HL 60 cells by a four hour exposure to 1 alpha, 25-dihydroxy vitamin D3 (1,25(OH)2D3). This treatment resulted in a rapid, selective, inhibition of DNA syntehsis, which was accompanied by reduced cellular levels of c-myc mRNA, and a more gradual appearance of c-fos mRNA. After removal of the inducer from the cultures, DNA syntehsis and c-myc mRNA levels returned rapidly to near-normal levels, but the expression of c-fos gene continued to increase for 24 hr and then declined slowly. Studies with isolated nuclei showed that the inhibition of DNA synthesis can be detected earlier than the changes in transcriptional rates of the oncogenes studied, and that 1,25(OH)2D3 directly inhibits the DNA synthesis in isolated nuclei. Autoradiographic studies of [3H] thymidine incorporation showed that 1,25(OH)2D3 does not immediately block the progression of the cells into the S phase of the cell cycle, but that those cells which become differentiated as the result of a brief exposure to this inducer do have such a block. It is concluded that 1,25(OH)2D3 produces both an immediate and a delayed inhibition of DNA synthesis in HL 60 cells, that the immediate inhibition is not preceded by detectable changes in oncogene expression, and that the delayed inhibition is accompanied by an elevated expression of the c-fos gene, and may be related to the monocytic differentiation of HL 60 cells.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041280206
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  • 10
    Electronic Resource
    Electronic Resource
    Thymidine as synchronizing agent. III. Persistence of cell cycle patterns of phosphatase activities and elevation of nuclease activity during inhibition of dna synthesis (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 297-303 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Synchronous cultures of HeLa cells were obtained by selective detachment of cells in mitosis and fluctuations in enzyme activity were followed during the subsequent cell cycle. The enzymes measured were alkaline and acid phosphatases and a nuclease active on denatured DNA at alkaline pH (alkaline DNase). Each of these enzymes showed a different pattern of activity in the cell cycle, but a temporal relationship to the DNA synthetic phase was apparent in each case. Treatment of the cultures at the beginning of the cell cycle with 15 mM thymidine did not alter the subsequent pattern of fluctuations in activity of alkaline phosphatase or of acid phosphatase, although DNA synthesis was fully inhibited by this treatment. This indicates that the pattern of activity of some enzymes is not linked to DNA replication. On the other hand, the pattern of fluctuations in the activity of alkaline DNase was abolished by thymidine treatment, and elevation of the activity of this enzyme was observed. These results suggest complex and variable relationships between phases of the cell cycle and enzyme activity, and show that inhibition of DNA synthesis is not a suitable procedure for induction of culture synchrony if enzyme activities are to be studied.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040750306
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