Publication Date:
2012-10-16
Description:
Cytoplasmic dynein and kinesin-1 are microtubule-based motors with opposite polarity that transport a wide variety of cargo in eukaryotic cells. Many cellular cargos demonstrate bidirectional movement due to the presence of ensembles of dynein and kinesin, but are ultimately sorted with spatial and temporal precision. To investigate the mechanisms that coordinate motor ensemble behavior, we built a programmable synthetic cargo using three-dimensional DNA origami to which varying numbers of DNA oligonucleotide-linked motors could be attached, allowing for control of motor type, number, spacing, and orientation in vitro. In ensembles of one to seven identical-polarity motors, motor number had minimal affect on directional velocity, whereas ensembles of opposite-polarity motors engaged in a tug-of-war resolvable by disengaging one motor species.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840815/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840815/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Derr, N D -- Goodman, B S -- Jungmann, R -- Leschziner, A E -- Shih, W M -- Reck-Peterson, S L -- 1 DP2 OD004268-1/OD/NIH HHS/ -- 1DP2OD004641/OD/NIH HHS/ -- 1U54GM094608/GM/NIGMS NIH HHS/ -- DP2 OD004268/OD/NIH HHS/ -- DP2 OD004641/OD/NIH HHS/ -- New York, N.Y. -- Science. 2012 Nov 2;338(6107):662-5. doi: 10.1126/science.1226734. Epub 2012 Oct 11.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23065903" target="_blank"〉PubMed〈/a〉
Keywords:
Cytoplasmic Dyneins/chemistry/*metabolism
;
DNA/*chemistry/*metabolism
;
DNA, Single-Stranded/chemistry/metabolism
;
Kinesin/chemistry/*metabolism
;
Kymography
;
Microtubules/*metabolism
;
Molecular Motor Proteins/chemistry/*metabolism
;
Nucleic Acid Conformation
;
Protein Multimerization
;
Saccharomyces cerevisiae Proteins/chemistry/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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