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  • 1
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    Electrohydrodynamic effects in continuous flow electrophoresis (1991)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: We demonstrate experimentally and theoretically the importance of electrohydrodynamic (EHD) flows in continuous-flow electrophoresis (CFE) separations. These flows are associated with variations in the conductivity or dielectric constant, and are quadratic in the field strength. They appear to be the main cause of extraneous and undesired flows in CFE which have degraded separation performance and have until now not been explained. We discuss the importance of EHD flows relative to other effects. We also describe possible techniques for reducing the associated degradation of CFE separations.
    Keywords: Life Sciences (General)
    Type: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society (ISSN 0954-6642); Volume 2; 2-3; 87-91
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  • 2
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    Stability of erythrocyte suspensions layered on stationary and flowing liquids (1982)
    In:  Other Sources
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    Publication Date: 2011-08-18
    Description: The apparent stability of erythrocyte suspensions layered on stationary and flowing Ficoll solutions was studied considering the effects of particle concentration, type and size, and the different flow rates of the particle suspensions and chamber liquid. The data from the flowing system were empirically fitted and, when extrapolated to zero chamber liquid flow rate, gave values comparable to the data from the stationary system, thus confirming the validity of the data and our approach to obtain that data.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: Separation Science and Technology; 17; 6, 19; 1982
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  • 3
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    Advances in electrophoretic separations (1984)
    In:  Other Sources
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    Publication Date: 2016-06-07
    Description: Free fluid electrophoresis is described using laboratory and space experiments combined with extensive mathematical modeling. Buoyancy driven convective flows due to thermal and concentration gradients are absent in the reduced gravity environment of space. The elimination of convection in weightlessness offers possible improvements in electrophoresis and other separation methods which occur in fluid media. The mathematical modeling suggests new ways of doing electrophoresis in space and explains various phenomena observed during past experiments. The extent to which ground based separation techniques are limited by gravity induced convection is investigated and space experiments are designed to evaluate specific characteristics of the fluid/particle environment. A series of experiments are proposed that require weightlessness and apparatus is developed that can be used to carry out these experiments in the near future.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
    Type: 2nd Symp. on Space Industrialization; p 368
    Format: text
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  • 4
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    Column electrophoresis on the Apollo-Soyuz test project. Experiment MA-011 (1977)
    In:  Other Sources
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    Publication Date: 2011-08-17
    Description: The experimental procedures of the electrophoresis of erythrocytes, lymphocytes and kidney cells on the Apollo Soyuz Test Project are given.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
    Type: Apollo-Soyuz Test Project; 68 p
    Format: text
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  • 5
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    Polystyrene latex separations by continuous flow electrophoresis on the Space Shuttle (1986)
    In:  Other Sources
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    Publication Date: 2011-08-19
    Description: The seventh mission of the Space Shuttle carried two NASA experiments in the McDonnell Douglas Astronautics Corporation continuous flow electrophoresis system. The objectives were to test the operation of continuous flow electrophoresis in a reduced gravity environment using stable particles with established electrokinetic properties and specifically to evaluate the influence of the electrical properties of the sample constituents on the resolution of the continuous flow electrophoretic device. Polystrene latex microspheres dispersed in a solution with three times the electrical conductivity of the curtain buffer separated with a significantly larger band spread compared to the second experiment under matched conductivity conditions. It is proposed that the sample of higher electrical conductivity distorted the electric field near the sample stream so that the polystyrene latex particles migrated toward the chamber walls where electroosmosis retarded and spread the sample.
    Keywords: MATERIALS PROCESSING
    Type: Separation Science and Technology (ISSN 0149-6395); 21; 2, 19
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  • 6
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    Flow and thermal effects in continuous flow electrophoresis (1979)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: In continuous flow electrophoresis the axial flow structure changes from a fully developed rectilinear form to one characterized by meandering as power levels are increased. The origin of this meandering is postulated to lie in a hydrodynamic instability driven by axial (and possibly lateral) temperature gradients. Experiments done at MSFC show agreement with the theory.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
    Type: Alabama Univ. UAH(NASA Workshop on Fluids Expt. System; p 136-145
    Format: application/pdf
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  • 7
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    Sample stream distortion modeled in continuous-flow electrophoresis (1979)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: Buoyancy-induced disturbances in an electrophoresis-type chamber were investigated. Five tracer streams (latex) were used to visualize the flows while a nine-thermistor array sensed the temperature field. The internal heating to the chamber was provided by a 400 Hz electrical field. Cooling to the chamber was provided on the front and back faces and, in addition, on both chamber side walls. Disturbances to the symmetric base flow in the chamber occurred in the broad plane of the chamber and resulted from the formation of lateral and axial temperature gradients. The effect of these gradients was to retard or increase local flow velocities at different positions in the chamber cross section, which resulted in lateral secondary flows being induced in the broad plane of the chamber. As the adverse temperature gradients increased in magnitude, the critical Rayleigh number was approached and reverse (separated) flow became apparent, which, subsequently, led to the onset of time variant secondary flows.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
    Type: NASA-TM-78178
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  • 8
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    Static continuous electrophoresis device (1982)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: An apparatus is disclosed for carrying out a moving wall type electrophoresis process for separation of cellular particles. The apparatus includes a water-tight housing containing an electrolytic buffer solution. A separation chamber in the housing is defined by spaced opposed moving walls and spaced opposed side walls. Substrate assemblies, which support the moving wall include vacuum ports for positively sealing the moving walls against the substrate walls. Several suction conduits communicate with the suction ports and are arranged in the form of valleys in a grid plate. The raised land portion of the grid plat supports the substrate walls against deformation inwardly under suction. A cooling chamber is carried on the back side of plate. The apparatus also has tensioner means including roller and adjustment screws for maintaining the belts in position and a drive arrangement including an electric motor with a gear affixed to its output shaft. Electrode assemblies are disposed to provide the required electric field.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
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  • 9
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    Improved Electrophoresis Cell (1982)
    In:  Other Sources
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    Publication Date: 2019-06-28
    Description: Several proposed modifications are expected to improve performance of a continous-flow electrophoresis cell. Changes would allow better control of buffer flow and would increase resolution by suppressing thermal gradients. Improved electrophoresis device would have high resolution and be easy to operate. Improvements would allow better flow control and heat dissipation.
    Keywords: LIFE SCIENCES
    Type: MFS-25426 , NASA Tech Briefs (ISSN 0145-319X); 6; 2; P. 173
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  • 10
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    High-resolution, continuous-flow electrophoresis in microgravity (1978)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: Approximate expressions which will allow estimation of performance enhancement in reduced gravity and will direct optimization of the various design parameters for a continuous flow electrophoresis system was devised. Other topics discussed include: (1) temperature and velocity distribution; (2) input power limitations in reduced gravity; and (3) effect of band distortion on resolution. A comparision of resolution for terrestrial and reduced gravity operations was also discussed.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
    Type: NASA-TM-78158
    Format: application/pdf
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