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  • 1
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    Triplet-repeat transcripts: a role for DNA in disease (1998)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1998-05-23
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Singer, R H -- New York, N.Y. -- Science. 1998 May 1;280(5364):696-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Structural Biology, Institute for Molecular Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA. rhsinger@aecom.yu.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9599147" target="_blank"〉PubMed〈/a〉
    Keywords: CELF1 Protein ; Cell Nucleus/metabolism ; Exons ; Humans ; Models, Genetic ; Myotonic Dystrophy/*genetics/metabolism ; Myotonin-Protein Kinase ; Protein Binding ; Protein-Serine-Threonine Kinases/*genetics ; *RNA Splicing ; RNA, Messenger/*genetics ; RNA-Binding Proteins/genetics/*metabolism ; Ribonucleoproteins/genetics/*metabolism ; Transcription, Genetic ; Transfection ; *Trinucleotide Repeats ; Troponin/genetics ; Troponin T
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
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    Single-cell gene expression profiling (2002)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2002-08-06
    Description: A key goal of biology is to relate the expression of specific genes to a particular cellular phenotype. However, current assays for gene expression destroy the structural context. By combining advances in computational fluorescence microscopy with multiplex probe design, we devised technology in which the expression of many genes can be visualized simultaneously inside single cells with high spatial and temporal resolution. Analysis of 11 genes in serum-stimulated cultured cells revealed unique patterns of gene expression within individual cells. Using the nucleus as the substrate for parallel gene analysis, we provide a platform for the fusion of genomics and cell biology: "cellular genomics."〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Levsky, Jeffrey M -- Shenoy, Shailesh M -- Pezo, Rossanna C -- Singer, Robert H -- GM54887/GM/NIGMS NIH HHS/ -- R33CA83208/CA/NCI NIH HHS/ -- T32GM07288/GM/NIGMS NIH HHS/ -- T32GM07491/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2002 Aug 2;297(5582):836-40.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12161654" target="_blank"〉PubMed〈/a〉
    Keywords: Adenocarcinoma/genetics ; Cell Nucleus/genetics ; Cells/*cytology/*metabolism ; Colonic Neoplasms/genetics ; Color ; DNA Probes ; Fibroblasts ; Gene Expression Profiling/instrumentation/*methods ; *Gene Expression Regulation ; Genes ; Genomics/instrumentation/methods ; Humans ; Microscopy, Fluorescence/instrumentation/*methods ; Odds Ratio ; RNA/genetics/metabolism ; Sensitivity and Specificity ; Time Factors ; Transcription, Genetic ; Tumor Cells, Cultured
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA (1997)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1997-07-18
    Description: Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells but not daughter cells can switch mating type because they selectively express the HO endonuclease gene. This asymmetry is due to the preferential accumulation of an unstable transcriptional repressor protein, Ash1p, in daughter cell nuclei. Here it is shown that ASH1 messenger RNA (mRNA) preferentially accumulates in daughter cells by a process that is dependent on actin and myosin. A cis-acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Long, R M -- Singer, R H -- Meng, X -- Gonzalez, I -- Nasmyth, K -- Jansen, R P -- 7 F32 HD08088-02/HD/NICHD NIH HHS/ -- GM54887/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1997 Jul 18;277(5324):383-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9219698" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/genetics/*physiology ; Cell Cycle ; Cell Nucleus/metabolism ; *DNA-Binding Proteins ; Deoxyribonucleases, Type II Site-Specific/genetics ; Fungal Proteins/genetics ; Genes, Fungal ; Genes, Mating Type, Fungal ; In Situ Hybridization, Fluorescence ; Microtubules/physiology ; Mutation ; *Myosin Heavy Chains ; *Myosin Type V ; Myosins/genetics ; RNA, Fungal/genetics/*metabolism ; RNA, Messenger/genetics/*metabolism ; Repressor Proteins/biosynthesis/*genetics ; Saccharomyces cerevisiae/cytology/genetics/metabolism/*physiology ; *Saccharomyces cerevisiae Proteins ; Transcription Factors/biosynthesis/*genetics ; Transformation, Genetic ; Tropomyosin/genetics/physiology ; Zinc Fingers
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Visualization of single RNA transcripts in situ (1998)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1998-05-09
    Description: Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized with five fluorochromes per molecule, and the light emitted by a single probe was calibrated. Points of light in exhaustively deconvolved images of hybridized cells gave fluorescent intensities and distances between probes consistent with single messenger RNA molecules. Analysis of beta-actin transcription sites after serum induction revealed synchronous and cyclical transcription from single genes. The rates of transcription initiation and termination and messenger RNA processing could be determined by positioning probes along the transcription unit. This approach extends the power of FISH to yield quantitative molecular information on a single cell.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Femino, A M -- Fay, F S -- Fogarty, K -- Singer, R H -- GM 54887/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1998 Apr 24;280(5363):585-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9554849" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/genetics ; Animals ; Cell Line ; Fluorescein-5-isothiocyanate ; *In Situ Hybridization, Fluorescence ; Kinetics ; Oligonucleotide Probes ; RNA Processing, Post-Transcriptional ; RNA, Messenger/*analysis/*genetics/metabolism ; Rats ; *Transcription, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
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    Interphase and metaphase resolution of different distances within the human dystrophin gene (1990)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1990-08-24
    Description: Fluorescence in situ hybridization makes possible direct visualization of single sequences not only on chromosomes, but within decondensed interphase nuclei, providing a potentially powerful approach for high-resolution (1 Mb and below) gene mapping and the analysis of nuclear organization. Interphase mapping was able to extend the ability to resolve and order sequences up to two orders of magnitude beyond localization on banded or unbanded chromosomes. Sequences within the human dystrophin gene separated by less than 100 kb to 1 Mb were visually resolved at interphase by means of standard microscopy. In contrast, distances in the 1-Mb range could not be ordered on the metaphase chromosome length. Analysis of sequences 100 kb to 1 Mb apart indicates a strong correlation between interphase distance and linear DNA distance, which could facilitate a variety of gene-mapping efforts. Results estimate chromatin condensation up to 1 Mb and indicate a comparable condensation for different cell types prepared by different techniques.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lawrence, J B -- Singer, R H -- McNeil, J A -- HD 18066/HD/NICHD NIH HHS/ -- HG 00251/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 1990 Aug 24;249(4971):928-32.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2203143" target="_blank"〉PubMed〈/a〉
    Keywords: Chromosome Banding ; Chromosome Mapping ; *Chromosomes, Human, Pair 1 ; DNA/genetics ; DNA Probes ; Dystrophin ; Fibroblasts/cytology ; *Genes ; Genome, Human ; Humans ; Interphase ; Metaphase ; Muscle Proteins/*genetics ; Nucleic Acid Hybridization
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    Requirement of microfilaments in sorting of actin messenger RNA (1991)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1991-09-13
    Description: Specific messenger RNAs (mRNAs) can be sequestered within distinct cellular locations, but little is known about how this is accomplished. The participation of the three major cellular filaments in the localization of actin mRNA was studied in chicken embryo fibroblasts. Movement of actin mRNA to the cell periphery and maintenance of that regionalization required intact microfilaments (composed of actin) but not microtubules or intermediate filaments. The results presented here suggest that actin-binding proteins may participate in mRNA sorting.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sundell, C L -- Singer, R H -- HD18066/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1991 Sep 13;253(5025):1275-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1891715" target="_blank"〉PubMed〈/a〉
    Keywords: Actin Cytoskeleton/drug effects/*physiology/ultrastructure ; Actins/*genetics ; Animals ; Cells, Cultured ; Chick Embryo ; Cytochalasin D/pharmacology ; Demecolcine/pharmacology ; Fibroblasts/cytology/physiology ; RNA, Messenger/analysis/drug effects/*genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    Real-time observation of transcription initiation and elongation on an endogenous yeast gene (2011)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2011-04-23
    Description: Cellular messenger RNA levels are achieved by the combinatorial complexity of factors controlling transcription, yet the small number of molecules involved in these pathways fluctuates stochastically. It has not yet been experimentally possible to observe the activity of single polymerases on an endogenous gene to elucidate how these events occur in vivo. Here, we describe a method of fluctuation analysis of fluorescently labeled RNA to measure dynamics of nascent RNA--including initiation, elongation, and termination--at an active yeast locus. We find no transcriptional memory between initiation events, and elongation speed can vary by threefold throughout the cell cycle. By measuring the abundance and intranuclear mobility of an upstream transcription factor, we observe that the gene firing rate is directly determined by trans-activating factor search times.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152976/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152976/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Larson, Daniel R -- Zenklusen, Daniel -- Wu, Bin -- Chao, Jeffrey A -- Singer, Robert H -- 57071/PHS HHS/ -- 86217/PHS HHS/ -- R01 GM057071/GM/NIGMS NIH HHS/ -- R01 GM057071-10/GM/NIGMS NIH HHS/ -- R01 GM057071-11/GM/NIGMS NIH HHS/ -- R01 GM057071-12/GM/NIGMS NIH HHS/ -- R01 GM086217/GM/NIGMS NIH HHS/ -- R01 GM086217-01/GM/NIGMS NIH HHS/ -- R01 GM086217-02/GM/NIGMS NIH HHS/ -- R01 GM086217-03/GM/NIGMS NIH HHS/ -- R01 GM086217-04/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2011 Apr 22;332(6028):475-8. doi: 10.1126/science.1202142.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21512033" target="_blank"〉PubMed〈/a〉
    Keywords: Adenosine Triphosphatases/genetics ; Cell Cycle ; Cell Nucleus/metabolism ; DNA Polymerase I/genetics ; Facilitated Diffusion ; *Genes, Fungal ; Glutamate Synthase/genetics ; Green Fluorescent Proteins ; Kinetics ; Microscopy, Fluorescence ; Models, Genetic ; Promoter Regions, Genetic ; RNA Polymerase II/metabolism ; RNA Precursors/genetics/metabolism ; RNA, Fungal/biosynthesis/*genetics ; RNA, Messenger/biosynthesis/*genetics ; Saccharomyces cerevisiae/*genetics/metabolism ; Saccharomyces cerevisiae Proteins/genetics/metabolism ; Spectrometry, Fluorescence ; Transcription Factors/metabolism ; *Transcription, Genetic ; Transcriptional Activation
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    Dynamics of single mRNPs in nuclei of living cells (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-06-19
    Description: Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA)expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4765737/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4765737/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shav-Tal, Yaron -- Darzacq, Xavier -- Shenoy, Shailesh M -- Fusco, Dahlene -- Janicki, Susan M -- Spector, David L -- Singer, Robert H -- DOE63056/PHS HHS/ -- EB2060/EB/NIBIB NIH HHS/ -- GM42694/GM/NIGMS NIH HHS/ -- R01 EB002060/EB/NIBIB NIH HHS/ -- R01 GM042694/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Jun 18;304(5678):1797-800.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15205532" target="_blank"〉PubMed〈/a〉
    Keywords: Active Transport, Cell Nucleus ; Adenosine Triphosphate/metabolism ; Capsid Proteins/genetics/metabolism ; Cell Line, Tumor ; Cell Nucleus/*metabolism ; Chromatin/metabolism ; Cytoplasm/metabolism ; Diffusion ; Energy Metabolism ; Fluorescence Recovery After Photobleaching ; Globins/genetics/metabolism ; Green Fluorescent Proteins ; Humans ; In Situ Hybridization, Fluorescence ; Luminescent Proteins/genetics/metabolism ; Peroxisomes/metabolism ; Protein Biosynthesis ; Proteins/genetics/*metabolism ; RNA, Messenger/genetics/*metabolism ; RNA-Binding Proteins/genetics/metabolism ; Recombinant Fusion Proteins/metabolism ; Transcription, Genetic ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
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    Nuclear export dynamics of RNA-protein complexes (2011)
    Nature Publishing Group (NPG)
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    Publication Date: 2011-07-22
    Description: The central dogma of molecular biology - DNA makes RNA makes proteins - is a flow of information that in eukaryotes encounters a physical barrier: the nuclear envelope, which encapsulates, organizes and protects the genome. Nuclear-pore complexes, embedded in the nuclear envelope, regulate the passage of molecules to and from the nucleus, including the poorly understood process of the export of RNAs from the nucleus. Recent imaging approaches focusing on single molecules have provided unexpected insight into this crucial step in the information flow. This review addresses the latest studies of RNA export and presents some models for how this complex process may work.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154952/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154952/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Grunwald, David -- Singer, Robert H -- Rout, Michael -- GM062427/GM/NIGMS NIH HHS/ -- GM071329/GM/NIGMS NIH HHS/ -- GM84364/GM/NIGMS NIH HHS/ -- GM86217/GM/NIGMS NIH HHS/ -- R01 GM057071/GM/NIGMS NIH HHS/ -- R01 GM062427/GM/NIGMS NIH HHS/ -- R01 GM062427-10/GM/NIGMS NIH HHS/ -- R01 GM062427-11/GM/NIGMS NIH HHS/ -- R01 GM071329/GM/NIGMS NIH HHS/ -- R01 GM071329-06/GM/NIGMS NIH HHS/ -- R01 GM071329-07/GM/NIGMS NIH HHS/ -- R01 GM084364/GM/NIGMS NIH HHS/ -- R01 GM084364-17/GM/NIGMS NIH HHS/ -- R01 GM084364-18/GM/NIGMS NIH HHS/ -- R01 GM086217/GM/NIGMS NIH HHS/ -- R01 GM086217-03/GM/NIGMS NIH HHS/ -- R01 GM086217-04/GM/NIGMS NIH HHS/ -- RR022220/RR/NCRR NIH HHS/ -- U54 RR022220/RR/NCRR NIH HHS/ -- U54 RR022220-06/RR/NCRR NIH HHS/ -- U54 RR022220-07/RR/NCRR NIH HHS/ -- England -- Nature. 2011 Jul 20;475(7356):333-41. doi: 10.1038/nature10318.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Delft University of Technology, Faculty of Applied Sciences, Kavli Institute of NanoScience, Department of Bionanoscience, 2628 CJ Delft, The Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21776079" target="_blank"〉PubMed〈/a〉
    Keywords: Active Transport, Cell Nucleus ; Cell Nucleus/*metabolism ; Cytoplasm/*metabolism ; Humans ; Microscopy ; Nuclear Pore/metabolism ; RNA/*metabolism ; Ribonucleoproteins/*metabolism ; Ribosomes/metabolism
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
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  • 10
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    Single beta-actin mRNA detection in neurons reveals a mechanism for regulating its translatability (2014)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2014-01-25
    Description: The physical manifestation of learning and memory formation in the brain can be expressed by strengthening or weakening of synaptic connections through morphological changes. Local actin remodeling underlies some forms of plasticity and may be facilitated by local beta-actin synthesis, but dynamic information is lacking. In this work, we use single-molecule in situ hybridization to demonstrate that dendritic beta-actin messenger RNA (mRNA) and ribosomes are in a masked, neuron-specific form. Chemically induced long-term potentiation prompts transient mRNA unmasking, which depends on factors active during synaptic activity. Ribosomes and single beta-actin mRNA motility increase after stimulation, indicative of release from complexes. Hence, the single-molecule assays we developed allow for the quantification of activity-induced unmasking and availability for active translation. Further, our work demonstrates that beta-actin mRNA and ribosomes are in a masked state that is alleviated by stimulation.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121734/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121734/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Buxbaum, Adina R -- Wu, Bin -- Singer, Robert H -- GM84364/GM/NIGMS NIH HHS/ -- NS083085-19/NS/NINDS NIH HHS/ -- R01 NS083085/NS/NINDS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2014 Jan 24;343(6169):419-22. doi: 10.1126/science.1242939.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24458642" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/*biosynthesis/genetics ; Animals ; Cells, Cultured ; Dendrites/metabolism ; In Situ Hybridization, Fluorescence/methods ; Long-Term Potentiation/drug effects/*physiology ; Memory/physiology ; Mice ; Mice, Transgenic ; Neuronal Plasticity/drug effects/physiology ; Neurons/*metabolism ; *Protein Biosynthesis ; RNA, Messenger/analysis/*biosynthesis ; RNA, Ribosomal/metabolism ; Ribosomes/*metabolism ; Synapses/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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