Publication Date:
2011-11-26
Description:
Evolutionarily old and conserved homeostatic systems in the brain, including the hypothalamus, are organized into nuclear structures of heterogeneous and diverse neuron populations. To investigate whether such circuits can be functionally reconstituted by synaptic integration of similarly diverse populations of neurons, we generated physically chimeric hypothalami by microtransplanting small numbers of embryonic enhanced green fluorescent protein-expressing, leptin-responsive hypothalamic cells into hypothalami of postnatal leptin receptor-deficient (db/db) mice that develop morbid obesity. Donor neurons differentiated and integrated as four distinct hypothalamic neuron subtypes, formed functional excitatory and inhibitory synapses, partially restored leptin responsiveness, and ameliorated hyperglycemia and obesity in db/db mice. These experiments serve as a proof of concept that transplanted neurons can functionally reconstitute complex neuronal circuitry in the mammalian brain.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770458/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770458/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Czupryn, Artur -- Zhou, Yu-Dong -- Chen, Xi -- McNay, David -- Anderson, Matthew P -- Flier, Jeffrey S -- Macklis, Jeffrey D -- DKR37-28082/PHS HHS/ -- K02 NS054674/NS/NINDS NIH HHS/ -- NS054674/NS/NINDS NIH HHS/ -- NS057444/NS/NINDS NIH HHS/ -- NS070295/NS/NINDS NIH HHS/ -- NS41590/NS/NINDS NIH HHS/ -- NS45523/NS/NINDS NIH HHS/ -- NS49553/NS/NINDS NIH HHS/ -- R01 NS041590/NS/NINDS NIH HHS/ -- R01 NS045523/NS/NINDS NIH HHS/ -- R01 NS049553/NS/NINDS NIH HHS/ -- R01 NS057444/NS/NINDS NIH HHS/ -- R21 NS070295/NS/NINDS NIH HHS/ -- R37 DK028082/DK/NIDDK NIH HHS/ -- R37 NS041590/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2011 Nov 25;334(6059):1133-7. doi: 10.1126/science.1209870.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Stem Cell and Regenerative Biology, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22116886" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blood Glucose/analysis
;
Body Weight
;
Cell Shape
;
Electrophysiological Phenomena
;
Excitatory Postsynaptic Potentials
;
Glucose/administration & dosage
;
Hypothalamus/*cytology/metabolism
;
Hypothalamus, Middle/*cytology/metabolism/*physiopathology
;
Inhibitory Postsynaptic Potentials
;
Insulin/administration & dosage/blood
;
Leptin/administration & dosage/*metabolism
;
Membrane Potentials
;
Mice
;
Mice, Obese
;
Neurogenesis
;
Neurons/cytology/*physiology/*transplantation
;
Obesity/metabolism/*physiopathology/*therapy
;
Receptors, Leptin/*metabolism
;
Signal Transduction
;
Synaptic Transmission
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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