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  • 1
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Two extraction procedures were examined, and it was found that DNA recovered from Campylobacter jejuni lysed by the cetyltrimethylammonium bromide (CTAB) method was more suitable for use as a PCR template than DNA released by the boiling method. The region targeted for PCR amplification was a 1.73-kb portion of the flagellin A gene of C. jejuni. The detection limit was lower than 30 cells per 100 ml in artificially contaminated waters. PCR assay and conventional culturing method had the same sensitivity, but results of the PCR technique were available within 48 h and so shortened the time necessary for detection by 48 h.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Diversity based on ribosomal RNA gene-restriction endonuclease digest patterns was detected amongst 42 strains of Campylobacter jejuni and 18 strains of C. coli including representatives of 53 different Penner serotypes. HaeIII ribopatterns were coded for numerical analysis which showed that all except two were different including those of several strains of the same serotype (P2 and P20). At the 30% similarity level, four groupings were formed in the analysis of which three corresponded to C. jejuni, C. coli and C. lari phenotypes respectively. Eight strains (13%) were atypical as their phenotypic and ribopattern associations did not correspond. Ribopattern fragments of 3.0, 5.0 and 9.3 kb were characteristic of the majority of C. jejuni, whereas 1.5, 2.2-, 2.3- and 4.7-kb fragments were commonly present in C. coli. These fragments provided novel species-specific markers. We conclude that HaeIII ribotyping was as discriminatory as Penner serotyping of C. jejuni and C. coli and may even provide a basis for distinguishing between strains of the same serotype and for identifying new groups within the thermophilic campylobacters.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this study, we evaluated the combination of polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and amplified fragment length polymorphism (AFLP) molecular typing techniques for the analysis of thermophilic campylobacter species isolated from clinical and poultry samples. 23S PCR–RFLP analysis performed to fingerprint 69 strains exhibited an excellent level of typability. Eleven different types were defined at 100% linkage level following numerical analysis of band patterns. Differentiation of Campylobacter jejuni and Campylobacter coli at species level was achieved although no significant relationship could be observed between the profiles and the origin of the strains. Simplified AFLP analysis of the isolates disclosed the presence of 66 different banding patterns. The resulting dendrogram showed a high diversity among the strains studied. All the isolates were grouped within eight main types with a 69% homology degree among them. Differentiation at subspecies level was possible but no significant relationship could be observed between the AFLP profiles and the origin of the strains. When used in combination, 23S PCR–RFLP and single-enzyme AFLP methods can be applied to determine taxonomic and epidemiological relationships among thermophilic campylobacters.
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of organic chemistry 60 (1995), S. 5180-5185 
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 17 (1997), S. 0 
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Arbitrary primed PCR (AP-PCR) analysis was compared with serotyping as a means of high-resolution typing of Pseudomonas aeruginosa. Seventy-four isolates from 3 different hospitals and 18 reference strains were studied. Serotyping provided good index of discrimination, although eleven isolates could not be serotyped. Genomic DNA was amplified with a single 10 nucleotide primer (sequence 5′-AGG GGT CTT G-3′). The strains were genetically diverse and 61 different AP-PCR profiles of 2–7 bands between 0.3 and 2.4 kb were obtained. AP-PCR profiles were not consistently associated with serotypes, but they clearly subtyped strains of the same serotype. Numerical analysis of AP-PCR patterns defined 7 groups at the 55% similarity level, and identified predominant strains in each hospital. The results show that AP-PCR analysis provides a simple and practical approach to typing P. aeruginosa that is more discriminatory than traditional serotyping scheme. We suggest that maximum discrimination can be achieved by a combination of both methods.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-0581
    Keywords: Drake Passage ; oceanic crust stratigraphy ; ridge-transform intersection ; tectonics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract New swath bathymetric, multichannel seismic and magnetic data reveal the complexity of the intersection between the extinct West Scotia Ridge (WSR) and the Shackleton Fracture Zone (SFZ), a first-order NW-SE trending high-relief ridge cutting across the Drake Passage. The SFZ is composed of shallow, ridge segments and depressions, largely parallel to the fracture zone with an `en echelon' pattern in plan view. These features are bounded by tectonic lineaments, interpreted as faults. The axial valley of the spreading center intersects the fracture zone in a complex area of deformation, where N120° E lineaments and E–W faults anastomose on both sides of the intersection. The fracture zone developed within an extensional regime, which facilitated the formation of oceanic transverse ridges parallel to the fracture zone and depressions attributed to pull-apart basins, bounded by normal and strike-slip faults. On the multichannel seismic (MCS) profiles, the igneous crust is well stratified, with numerous discontinuous high-amplitude reflectors and many irregular diffractions at the top, and a thicker layer below. The latter has sparse and weak reflectors, although it locally contains strong, dipping reflections. A bright, slightly undulating reflector observed below the spreading center axial valley at about 0.75 s (twt) depth in the igneous crust is interpreted as an indication of the relict axial magma chamber. Deep, high-amplitude subhorizontal and slightly dipping reflections are observed between 1.8 and 3.2 s (twt) below sea floor, but are preferentially located at about 2.8–3.0 s (twt) depth. Where these reflections are more continuous they may represent the Mohorovicic seismic discontinuity. More locally, short (2–3 km long), very high-amplitude reflections observed at 3.6 and 4.3 s (twt) depth below sea floor are attributed to an interlayered upper mantle transition zone. The MCS profiles also show a pattern of regularly spaced, steep-inclined reflectors, which cut across layers 2 and 3 of the oceanic crust. These reflectors are attributed to deformation under a transpressional regime that developed along the SFZ, shortly after spreading ceased at the WSR. Magnetic anomalies 5 to 5 E may be confidently identified on the flanks of the WSR. Our spreading model assumes slow rates (ca. 10–20 mm/yr), with slight asymmetries favoring the southeastern flank between 5C and 5, and the northwestern flank between 5 and extinction. The spreading rate asymmetry means that accretion was slower during formation of the steeper, shallower, southeastern flank than of the northwestern flank.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 70 (1998), S. 951-960 
    ISSN: 0020-7608
    Keywords: Chemistry ; Theoretical, Physical and Computational Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The structural, electronic, vibrational, and topologic properties of a series of acid sites of zeolites were studied at different levels of ab initio molecular orbital theory. The zeolite acid sites were modeled by using the following molecular clusters: silanol H3SiOH (B0) and the clusters H3SiO(H)AlH3 (B1), (OH)3SiO(H)Al(OH)3 (B1―OH), and H3SiO(H)Al(OH)2SiH3(B2). The calculation of geometries and properties of these clusters were performed at the Hartree-Fock level, and, additionally, second-order Møller-Plesset (MP2) and density functional BLYP calculations were carried out for silanol and B1 clusters. Geometries were fully optimized by following Cs symmetry restrictions. The standard STO/6-31+G(D, P) basis set, which includes polarization and diffuse functions, was used for all the calculations. The topologic properties of the zeolite acid clusters, based on the theory of atoms in molecules, were analyzed in terms of the total density and the Laplacian density properties, both evaluated at the position of the bond critical points. The calculations showed that the frequency of the OH vibrational modes of the zeolite acid sites, often used as an infrared index for characterizing the acidity of zeolites, is linearly related to the total density of the charge at the critical points of the OH bonds, with a correlation coefficient of r2=0.97. These results indicate that the total density of the electronic charge at the critical point of the OH bond can be used as a tool for interpreting the structural and electronic features of the zeolite hydroxyl groups. A relationship between the Mulliken population of the H atom of the OH bond and the OH frequency gives a correlation coefficient of 0.67. On the other hand, the values of the Laplacian density calculated at the critical points of the bonds of the acid sites indicate that the zeolite structure is dominated by a network of Si—O and Al—O ionic interactions, while the O(SINGLE BOND)H bonds are characterized as covalent bonds, with different extents of charge concentration.   © 1998 John Wiley & Sons, Inc. Int J Quant Chem 70: 951-960, 1998
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 2015-06-29
    Description: We describe Collinsium ciliosum from the early Cambrian Xiaoshiba Lagerstätte in South China, an armored lobopodian with a remarkable degree of limb differentiation including a pair of antenna-like appendages, six pairs of elongate setiferous limbs for suspension feeding, and nine pairs of clawed annulated legs with an anchoring function. Collinsium belongs to a highly derived clade of lobopodians within stem group Onychophora, distinguished by a substantial dorsal armature of supernumerary and biomineralized spines (Family Luolishaniidae). As demonstrated here, luolishaniids display the highest degree of limb specialization among Paleozoic lobopodians, constitute more than one-third of the overall morphological disparity of stem group Onychophora, and are substantially more disparate than crown group representatives. Despite having higher disparity and appendage complexity than other lobopodians and extant velvet worms, the specialized mode of life embodied by luolishaniids became extinct during the Early Paleozoic. Collinsium and other superarmored lobopodians exploited a unique paleoecological niche during the Cambrian explosion.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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