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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of wheat (Triticum aestivum L.) through electroporation of protoplasts (1994)
    Springer
    Plant cell reports 14 (1994), S. 192-196 
    Details
    ISSN: 1432-203X
    Keywords: gene transfer ; selection for phosphinothricin resistance ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts isolated from embryogenic suspension cultures of wheat (Triticum aestivum cv. Hartog) were electroporated in the presence of plasmid pEmuGN and/or pEmuPAT, which contained the reporter gene gus and selectable marker gene bar, respectively. Under optimised electroporation conditions, up to 0.9% of viable protoplasts displayed gus activity two days after electroporation. To select for phosphinothricin (PPT) resistant colonies, electroporated protoplasts were incubated for six weeks in a medium containing 10 μg/ml PPT. The cells surviving the selection were maintained as individual colonies on solid medium or as suspension cultures. More than 60% of these colonies exhibited tolerance to 40 μg/ml PPT when tested 10 months after initial selection. To date, 57 green plants have been regenerated from these colonies and 24 have been transferred to soil. Southern blot analyses of colonies and plants, using the bar gene sequence as the probe, confirmed transformation of the cells. Positive PAT assays of both regenerated colonies and plants indicated the presence of the bar gene product. These results provide a basis for the establishment of routine procedures for transformation of wheat by direct gene transfer into protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233789
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cell aggregates in wheat suspension cultures and their effects on isolation and culture of protoplasts (1994)
    Springer
    Plant cell reports 13 (1994), S. 176-179 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fine embryogenic suspension cultures of wheat (Triticum aestivum cv Hartog and Timmo, and T. durum cv D6962) tend to grow into large cell clumps (1–3 mm), resulting in the formation of mixed suspension cultures consisting of both fine and large cell clumps. The cell clumps were separated according to their sizes and cultured as new lines to investigate their growth rate and differentiation potential and the effects of cell aggregate size on protoplast culture. The results showed that the fine clusters (〈310 μm) had a higher growth rate but a lower differentiation frequency than the large cell aggregates (310–2000 μm). After 2–4 weeks incubation, all the new lines reformed mixed suspension cultures again. The large clumps (〉1100 μm) released fine cell clusters into the medium so it was possible to initiate fine embryogenic suspension cultures from the large clumps. With regard to the isolation and culture of protoplasts, although the highest yield of protoplasts was obtained from the fine cell clusters, the protoplasts isolated from different sized cell aggregates all had similar potential for sustained cell division and plant regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239887
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog) (1992)
    Springer
    Plant cell reports 11 (1992), S. 16-19 
    Details
    ISSN: 1432-203X
    Keywords: Embryogenesis ; Plant regeneration ; Protoplast ; Suspension cultures ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium containing half strength of the macroelements, 5 μm 2,4-D and 0.6 M glucose. Colonies were formed at frequencies ranging from 0.1% to 5%. The frequency of colonies forming fully developed plants varied between 1% and 25%. More than eighty green plants with morphologically normal shoots and roots have been obtained and there was no difficulty in establishing these plants in soil. A cytological study of several randomly selected regenerated plants showed the normal chromosome complement for wheat (2n = 42).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231832
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  • 4
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    Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells (2014)
    Springer Nature
    Details
    Publication Date: 2014-01-31
    Description: Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells Cell Death and Disease 5, e1043 (January 2014). doi:10.1038/cddis.2014.5 Authors: V Pavet, Y Shlyakhtina, T He, D G Ceschin, P Kohonen, M Perälä, O Kallioniemi & H Gronemeyer
    Keywords: Apo2L/TRAIL/TNFSF10PLAUuPAapoptosis
    Electronic ISSN: 2041-4889
    Topics: Biology , Medicine
    Published by Springer Nature
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