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  • 1
    Digitale Medien
    Digitale Medien
    Antioxidant Enzymes in Peroxisomes: Effect of Ischemia (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 804 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb18671.x
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  • 2
    Digitale Medien
    Digitale Medien
    Environmental management and its impact on the operations function (1995)
    Bingley : Emerald
    International journal of operations & production management 15 (1995), S. 34-51 
    Details
    ISSN: 0144-3577
    Quelle: Emerald Fulltext Archive Database 1994-2005
    Thema: Wirtschaftswissenschaften
    Notizen: Recently, corporations have been confronted with a number of globalenvironmental challenges such as global warming, acid rain, depletion ofnatural resources, waste management, green consumerism and pollutionprevention. There is growing pressure to deliver products and serviceswhich are environmentally compatible. A number of corporations such asDu Pont, 3M, AT&T, Xerox and Procter & Gamble are, therefore,integrating various environmental policies and programmes into theiroperations strategy and specific decisions concerning operations such asproduct design/planning, process technology selection, and qualitymanagement. Introduces the concepts of environmental management (EM) andargues that firms which do not recognize the implications ofenvironmental problems on the operations function will not succeed inthe competitive market. Various environmental management practices (suchas implementing aggressive pollution-prevention programmes, initiatingenvironment-related performance measures and developing green productsand process technologies) provide opportunities to strengthen a firm'sdistinctive competence in terms of operations objectives such as highestquality, lowest cost, best dependability, and greatest flexibility.Thus, EM gives a competitive advantage and develops new links betweenoperations strategy and the corporate strategy (e.g. cost leadership andproduct differentiation).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1108/01443579510094071
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  • 3
    Digitale Medien
    Digitale Medien
    Constraints management: What is the theory? (2004)
    Bingley : Emerald
    International journal of operations & production management 24 (2004), S. 350-371 
    Details
    ISSN: 0144-3577
    Quelle: Emerald Fulltext Archive Database 1994-2005
    Thema: Wirtschaftswissenschaften
    Notizen: A number of attempts have been made to develop theories in operations management (OM) (e.g. trade-off theory by Skinner, customer-contact model by Chase and Tansik, product-process matrix by Hayes and Wheelwright). Researchers in OM acknowledge that there is no widely-accepted theory on which OM rests or which serves as a unified OM theory to integrate existing theory-like principles or informal theories. Constraints management (CM) has been developed over the past 20 years by consultants and practitioners but has received little attention from OM researchers. The authors believe that constraints management may serve as a broad theory within operations that will allow integration of a great deal of existing OM research. The main objectives of this paper are to propose a construct, throughput orientation, discuss its core dimensions, and develop a theoretical model of CM. The paper also suggests several hypotheses that might be empirically tested to establish CM as a recognized theory in the field of operations management. The paper concludes with suggestions for future research.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1108/01443570410524631
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Catecholamines and cardiac growth (1996)
    Springer
    Molecular and cellular biochemistry 163-164 (1996), S. 203-210 
    Details
    ISSN: 1573-4919
    Schlagwort(e): adrenergic system ; control of gene expression ; α-myosin heavy chain
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The present knowledge concerning the α- and β-adrenergic systems in the regulation of cardiac growth and gene expression in reviewed. To investigate the mechanism by which CAMP regulates the expression of cardiac genes we have used cultured myocytes derived from fetal rat hearts. We have shown previously that the addition of Br cAMP to the culture medium produced an increase in α-myosin heavy chain (α-MHC) mRNA level, in its rate of transcription as well as in the amount of V1 isomyosin. To characterize the promoter element(s) involved in cAMP responsive regulation of a-MHC expression we performed transient transfection analysis with a series of α-MHC gene promoter-CAT constructs. We have identified a 13 by E-box/M-CAT hybrid motif (EM element) which conferred a basal muscle specific and cAMP inducible expression of the α-MHC gene. Using mobility shift assay we have documented that one of the EM element binding protein is TEF-1. Moreover, by incubating cardiac nuclear extracts with the catalytic subunit of PK-A we have found that factor(s) binding to the EM element is a substrate for CAMP dependent phosphorylation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00408659
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Cardiac hypertrophy: Old concepts, new perspectives (1997)
    Springer
    Molecular and cellular biochemistry 176 (1997), S. 273-279 
    Details
    ISSN: 1573-4919
    Schlagwort(e): cardiac hypertrophy ; myosin heavy chain ; gene expression ; adrenergic system
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Growth of the heart in hypertrophy is accompanied by changes in the phenotypic expression of cardiac genes. To explore the molecular basis of cardiac hypertrophy, we have analyzed the regulation of myosin heavy chain gene (MHC) expression. In one set of experiments, pressure overload on the rat heart was produced by constriction of the abdominal aorta. Changes in the α and β-MHC mRNA were then studied in overloaded hearts and following load removal. Pressure overload resulted in down-regulation of the α-MHC with corresponding up-regulation of the steady state level of β-MHC mRNA. Load removal (debanding) resulted in regression of cardiac hypertrophy and a rapid return of α-MHC mRNA to normal values. In contrast, the recovery in β-MHC mRNA was much slower to the extent that it remained substantially elevated compared to respective sham controls even after 7 weeks of post-debanding. These results suggest that putative load-related signals independently regulate two genes. Several lines of evidence indicate that adrenergic nervous system plays an important role in the induction and maintenance of cardiac hypertrophy and in the redistribution of myosin isoforms. We have analyzed the effect of cAMP inducing agents on the regulation of a-MHC gene in primary cultures of the fetal (18 day) rat cardiac myocyte. Inclusion of 8 Br-cAMP in the culture media increased the expression of α-MHC promoter/reporter construct comprising of 2.9 kb upstream sequence of the α-MHC gene. Several deletion mutations in the α- MHC gene promoter defined the cAMP responsive boundaries to be a 32 bp region comprising of -71 to -40 bp sequences. Deletion of this region resulted in loss of cAMP response as well as in basal expression of α-MHC promoter/reporter construct. These data suggest a role of β-adrenergic pathway in the modulation of α-MHC gene expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006865515646
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  • 6
    Digitale Medien
    Digitale Medien
    Dexamethasone induced cardiac hypertrophy in newborn rats is accompanied by changes in myosin heavy chain phenotype and gene transcription (2000)
    Springer
    Molecular and cellular biochemistry 209 (2000), S. 165-174 
    Details
    ISSN: 1573-4919
    Schlagwort(e): myosin heavy chain ; gene expression ; hypertrophy ; dexamethasone ; promoter function
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Cardiac hypertrophy has been observed in newborn infants treated with dexamethasone (DEX). This study was undertaken to examine whether DEX-induced hypertrophy in newborn rats is associated with redistribution of cardiac myosin heavy chain (MHC) isoforms and if so, the effects involve transcriptional regulation. Newborn rats were injected with either DEX (1 mg/kg/day; s.c.) or equivalent volume normal saline for 1, 3, 5, 7 or 9 days. Hypertrophy was quantified by heart dry/wet wt ratios, heart/body wt ratios, and total protein content of the myocardium. Changes in the expression of cardiac MHC mRNA were characterized by northern blot and slot blot analyses, using isoform specific probes for a- and β-MHC genes. DEX effect on α-MHC gene transcription was analyzed by transiently transfecting various α-MHC promoter/CAT reporter constructs into primary cultures of cardiac myocytes derived from one day old rat pups. DEX administration into newborn rats produced significant cardiac hypertrophy ranging from 23% at day 1 to 59% at 9 days. The hypertrophy was accompanied by immediate increase (83%) in steady state level of the α-MHC mRNA within one day and a maximum increase (148%) at 7 days of treatment. The steady state level of β-MHC mRNA declined by 25% at day 1 and a maximum decrease of 54% at day 7 of DEX treatment. The changes in MHC mRNA were also reflected in their protein levels as determined by V1 and V3 isozyme analysis. DEX treatment of primary cultures of cardiomyocytes following transfection with a-MHC promoter/CAT reporter constructs resulted in increased CAT expression in a dose dependent manner. The minimum α-MHC gene sequences responding to DEX treatment were located between the -200 to -74-bp region of the gene, resulting in 2-fold and 6-fold activation of CAT reporter after 0.05 and 0.1 mM doses of DEX, respectively. Our data indicate that DEX induced cardiac hypertrophy in newborn rats is accompanied by increased expression of α-MHC and decreased expression of β-MHC. The α-MHC effects are mediated in part through transcriptional mechanisms.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1007128300430
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  • 7
    Digitale Medien
    Digitale Medien
    Effects of exercise training and diabetes on cardiac myosin heavy chain composition (1992)
    Springer
    Molecular and cellular biochemistry 117 (1992), S. 175-179 
    Details
    ISSN: 1573-4919
    Schlagwort(e): diabetes ; diabetic cardiomyopathy ; myosin ; messenger RNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract This study determined whether the beneficial effects of exercise training on the diabetic heart previously observed are associated with alterations in ventricular myosin heavy chain (MHC) isoform composition. Diabetes was induced in rats by i.v. streptozotocin. Trained rats were run on a treadmill for 60 min/day, 27 m/min, 10% grade. After 10 wks, ventricular MHC isoenzyme protein composition was analyzed for MHC composition using gel electrophoresis. α-MHC and β-MHC mRNA were determined by Northern and slot blot hybridization techniques. Both protein and mRNA analyses indicated that sedentary control rats exhibited a predominance of α-MHC. Sedentary diabetics exhibited a shift to β-MHC. Exercise trained diabetic rats showed a predominance of β-MHC. The results indicate that treadmill exercise training of diabetic rat does not prevent the diabetes-induced shift in MHC composition towards the β-MHC isoform, thus it is unlikely that the beneficial effects of exercise training on the diabetic heart, previously shown, are due to a normalization of the myosin isoform composition.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00230757
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Sympathetic control of cardiac myosin heavy chain gene expression (1996)
    Springer
    Molecular and cellular biochemistry 157 (1996), S. 117-124 
    Details
    ISSN: 1573-4919
    Schlagwort(e): cardiac myocytes ; myosin heavy chain gene ; cAMP ; phosphorylation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Several neuroendocrine factors have been shown to influence the muscle phenotype. Various physiological reports have suggested the role of adrenergic nervous system for cardiac myosin heavy chain (MHC) expression. We have used cultured fetal rat heart myocytes to investigate the role of cAMP on the α- and (β-MHC gene expression. In low density cultures, addition of 1 mM 8 Br CAMP resulted in up regulation of α-MHC and down regulation of β-MHC mRNA. This antithetic effect of cAMP depends on the basal expression of both MHC transcripts. In transient transfection analysis employing a series of α-MHC gene promoter/reporter constructs, we identified a 13 by E-box M-CAT hybrid motif (EM element) which conferred a basal muscle specific and cAMP- inducible expression of the α-MHC gene. Data obtained from the mobility gel-shift analysis indicated that one of the factor(s) binding to the EM element is related to troponin T M-CAT binding factor (TEF-1). To test whether the protein binding to this sequence could be a substrate for cAMP-dependent phosphorylation, the cardiac nuclear proteins were preincubated in a kinase reaction buffer either with a catalytic subunit of PKA (CatPKA) or with cAMP, and binding activity of proteins to the EM element was evaluated by mobility gel shift assay. In a concentration dependent manner, a twofold increase in the intensity of the retarded band was observed. Furthermore, at 100 units of CatPKA, an additional band of faster mobility was observed which was not present either when phosphorylated nuclear extract was incubated with alkaline phosphatase or when ATP was absent in kinase reaction buffer. These results strongly suggest that factor(s) binding to the EM element is a substrate for cAMP dependent phosphorylation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00227889
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Manganese superoxide dismutase in rat liver peroxisomes: Biochemical and immunochemical evidence (1999)
    Springer
    Molecular and cellular biochemistry 197 (1999), S. 7-12 
    Details
    ISSN: 1573-4919
    Schlagwort(e): peroxisomes ; superoxide dismutase ; antioxidant enzymes ; free radicals ; immunolocalization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract By using highly purified peroxisomes from rat liver, we have shown that peroxisomes contain manganese superoxide dismutase (MnSOD) activity and a 23 kDa protein immunoreactive with antibodies against purified mitochondrial MnSOD. Immunocytochemical studies have also revealed immunoreaction (immunogold) with MnSOD antibodies in mitochondria and peroxisomes. Studies of the intraperoxisomal localization of MnSOD have shown that in peroxisomes MnSOD is a component of the peroxisomal limiting membranes and dense core. Furthermore, the MnSOD level in peroxisomes was modulated by oxidative stress conditions such as ischemia-reperfusion or the treatment with ciprofibrate, a peroxisomal proliferator. These findings suggest that MnSOD in peroxisomes may play an important role in the dismutation of superoxide generated on the peroxisomal membrane for keeping the delicate balance of the redox state.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006848113499
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  • 10
    Digitale Medien
    Digitale Medien
    Studies on hepatic injury and antioxidant enzyme activities in rat subcellular organelles following in vivo ischemia and reperfusion (1997)
    Springer
    Molecular and cellular biochemistry 176 (1997), S. 337-347 
    Details
    ISSN: 1573-4919
    Schlagwort(e): antioxidant enzymes ; sub-cellular organelles ; liver ; ischemia-reperfusion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The activities of rat hepatic subcellular antioxidant enzymes were studied during hepatic ischemia/reperfusion. Ischemia was induced for 30 min (reversible ischemia) or 60 min (irreversible ischemia). Ischemia was followed by 2 or 24 h of reperfusion. Hepatocyte peroxisomal catalase enzyme activity decreased during 60 min of ischemia and declined further during reperfusion. Peroxisomes of normal density (d = 1.225 gram/ml) were observed in control tissues. However, 60 min of ischemia also produced a second peak of catalase specific activity in subcellular fractions corresponding to newly formed low density immature peroxisomes (d = 1.12 gram/ml). The second peak was also detectable after 30 min of ischemia followed by reperfusion for 2 or 24 h. Mitochondrial and microsomal fractions responded differently. MnSOD activity in mitochondria and microsomal fractions increased significantly (p 〈 0.05) after 30 min of ischemia, but decreased below control values following 60 min of ischemia and remained lower during reperfusion at 2 and 24 h in both organelle fractions. Conversely, mitochondrial and microsomal glutathione peroxidase (GPx) activity increased significantly (p 〈 0.001) after 60 min of ischemia and was sustained during 24 h of reperfusion. In the cytosolic fraction, a significant increase in CuZnSOD activity was noted following reperfusion in animals subjected to 30 min of ischemia, but 60 min of ischemia and 24 h of reperfusion resulted in decreased CuZnSOD activity. These studies suggest that the antioxidant enzymes of various subcellular compartments respond to ischemia/reperfusion in an organelle or compartment specific manner and that the regulation of antioxidant enzyme activity in peroxisomes may differ from that in mitochondria and microsomes. The compartmentalized changes in hepatic antioxidant enzyme activity may be crucial determinant of cell survival and function during ischemia/reperfusion. Finally, a progressive decline in the level of hepatic reduced glutathione (GSH) and concomitant increase in serum glutamate pyruvate transaminase (SGPT) activity also suggest that greater tissue damage and impairment of intracellular antioxidant activity occur with longer ischemia periods, and during reperfusion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006829902442
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