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  • 1
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    Synchronizing nuclear import of ribosomal proteins with ribosome assembly (2012)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2012-11-03
    Description: Ribosomal proteins are synthesized in the cytoplasm, before nuclear import and assembly with ribosomal RNA (rRNA). Little is known about coordination of nucleocytoplasmic transport with ribosome assembly. Here, we identify a transport adaptor, symportin 1 (Syo1), that facilitates synchronized coimport of the two 5S-rRNA binding proteins Rpl5 and Rpl11. In vitro studies revealed that Syo1 concomitantly binds Rpl5-Rpl11 and furthermore recruits the import receptor Kap104. The Syo1-Rpl5-Rpl11 import complex is released from Kap104 by RanGTP and can be directly transferred onto the 5S rRNA. Syo1 can shuttle back to the cytoplasm by interaction with phenylalanine-glycine nucleoporins. X-ray crystallography uncovered how the alpha-solenoid symportin accommodates the Rpl5 amino terminus, normally bound to 5S rRNA, in an extended groove. Symportin-mediated coimport of Rpl5-Rpl11 could ensure coordinated and stoichiometric incorporation of these proteins into pre-60S ribosomes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kressler, Dieter -- Bange, Gert -- Ogawa, Yutaka -- Stjepanovic, Goran -- Bradatsch, Bettina -- Pratte, Dagmar -- Amlacher, Stefan -- Strauss, Daniela -- Yoneda, Yoshihiro -- Katahira, Jun -- Sinning, Irmgard -- Hurt, Ed -- New York, N.Y. -- Science. 2012 Nov 2;338(6107):666-71. doi: 10.1126/science.1226960.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biochemie-Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg D-69120, Germany. dieter.kressler@unifr.ch〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23118189" target="_blank"〉PubMed〈/a〉
    Keywords: *Active Transport, Cell Nucleus ; Amino Acid Sequence ; Base Sequence ; Cell Nucleus/*metabolism ; Chaetomium/metabolism ; Crystallography, X-Ray ; Fungal Proteins/chemistry/metabolism ; Models, Molecular ; Molecular Sequence Data ; Protein Binding ; Protein Conformation ; Protein Multimerization ; Protein Structure, Tertiary ; RNA, Fungal/metabolism ; RNA, Ribosomal, 5S/metabolism ; RNA-Binding Proteins/chemistry/*metabolism ; Ribosomal Proteins/chemistry/*metabolism ; Ribosomes/*metabolism ; Saccharomyces cerevisiae/*metabolism ; Saccharomyces cerevisiae Proteins/chemistry/*metabolism ; beta Karyopherins/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Effects of Substrate Heating and Wettability on Evaporation Dynamics and Deposition Patterns for a Sessile Water Droplet Containing Colloidal Particles (2016)
    American Chemical Society (ACS)
    In: Langmuir
    Details
    Publication Date: 2016-11-02
    Description: Langmuir DOI: 10.1021/acs.langmuir.6b02769
    Print ISSN: 0743-7463
    Electronic ISSN: 1520-5827
    Topics: Chemistry and Pharmacology
    Published by American Chemical Society (ACS)
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  • 3
    Electronic Resource
    Electronic Resource
    Comparison of Compartmental Analysis for Rb+ and Na+ in Low- and High-Salt Barley Roots (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 46 (1979), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The size of the cytoplasmic pools of Rb+ and Na+ in roots of barley (Hordeum vulgare L.) was estimated by two independent methods:〈list style="custom"〉1. from the time curves of net Rb+ and Na+ uptake in low-salt roots;2. from the kinetics of ion exchange in Rb+-and Na+-saturated roots.The results indicate that in low-salt roots there is a lag-phase in vacuolar accumulation of Rb+ but probably not of Na+. At the same time part of the cytoplasmic Na+ pool seems to be excluded from rapid exchange. Both for Rb+ and Na+, flux reduction in saturated tissue appears to be more pronounced at the tonoplast than at the plasmalemma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1979.tb06554.x
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  • 4
    Electronic Resource
    Electronic Resource
    Divalent Cations and K/Na Selectivity in Excised Barley Roots (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 34 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of Ca2+ and Mg2+ on the rate of K+ (Na+) uptake from different external concentrations ranging from 0 to 1 mM and in the absence and presence of a fixed concentration of Na+ (K+) in the same range, was determined. The object was to examine to what degree the qualitatively well-established action of the polyvalent cations on these processes could be understood quantitatively from a twofold starting-point: in the first place the theory of carrier competition (Bange 1962, Acta Bot. Neerl. 11: 139–146) and secondly the hypothesis that polyvalent cations influence carrier site accessibility at the cell surface.It appears that along these lines a semi-quantitative description of the phenomena observed is feasible. Possible causes of deviations are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb03802.x
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  • 5
    Electronic Resource
    Electronic Resource
    Localization of inorganic ions by precipitative freeze dissolution (1978)
    Springer
    Histochemistry and cell biology 55 (1978), S. 81-96 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new method for localization of inorganic diffusible ions in tissue is introduced. It has been applied to localization of Tl+ and Rb+ in barley roots and is probably also suited for Cs+, Ca2+, Cl−, Br−, PO 4 3− and perhaps K+. Its principle consists of dissolution of the ice from frozen tissue in a concentrated aqueous solution of a precipitating agent that is kept at a temperature just above its melting point.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493511
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  • 6
    Electronic Resource
    Electronic Resource
    Translocation of potassium and sodium in intact maize seedlings (1961)
    Springer
    Plant and soil 15 (1961), S. 312-328 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The distribution of K and Na between root and shoot of intact maize seedlings after absorption periods of increasing length up to 24 hours was determined in three series of experiments. In two additional series the redistribution between root and shoot of K and Na absorbed previously during a period of 12 hours was followed up during the next 12 hours. Concentrations were such that either the specific or both the specific and non-specific mechanism of K-absorption present in this tissue were operative. A high degree of specificity in the transfer to the shoot became apparent, the amount of Na transported to the shoot within 24 hours being negligible in comparison to the amount of K transferred within the same period. For the most part this specificity appeared to be due to the fact that the specific mechanism of K-absorption is especially involved in the supply of K for transfer to the shoot. However, non-specifically absorbed K is also liable to transport to the aerial parts at a much higher rate than Na and, in contrast to Na, the translocation of K continues after the plants have been transferred to K- and Na-free media. A scheme is proposed to explain the phenomena described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01379466
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  • 7
    Electronic Resource
    Electronic Resource
    The initial phase of ion uptake by plant roots (1963)
    Springer
    Plant and soil 18 (1963), S. 85-98 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experimental evidence is advanced which contradicts the view that the intercepts on the ordinate of plots of amount of cation absorbedvs time may be interpreted as representing the amount of ion initially bound to the sites involved in its active absorption and may be used to calculate the concentration of these sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01391683
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  • 8
    Electronic Resource
    Electronic Resource
    Upward transport of potassium in maize seedlings (1965)
    Springer
    Plant and soil 22 (1965), S. 280-306 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Upward K-translocation was compared in intact and decapitated maize seedlings. Shoot excision appeared to result in a serious reduction of upward K-transfer, whereas root K-content was hardly affected. A reduction in the rate of a metabolically-controlled process could be shown to be responsible for the effect. This process was identified as the transfer of K from the root cells to the sap. The inference that the transition of K from the root cells to the sap is under metabolic control, appears to be readily reconcilable with the concept of a selective transfer of K across the root developed in a previous study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01373997
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  • 9
    Electronic Resource
    Electronic Resource
    A comparison of the effect of calcium and magnesium on the separate components of alkali cation uptake in excised barley roots (1968)
    Springer
    Plant and soil 28 (1968), S. 177-181 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In excised barley roots the presence of either Ca or Mg reduces the additional amount of Na taken up within the first few hours after immersion in a Na-solution, presumably by blocking the free penetration of Na through the outer cell barrier. In contrast, only Ca is effective in shifting the ratio in which Rb and Na are absorbed from a mixture of the two ions, the shift favouring Rb. Therefore, a direct although still unexplained action of Ca on the system involved in the absorption of monovalent cations, independent of effects resulting from its influence on cell permeability, is advocated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01349188
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  • 10
    Electronic Resource
    Electronic Resource
    Interactions in the potassium and sodium absorption by intact maize seedlings (1959)
    Springer
    Plant and soil 11 (1959), S. 17-29 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relation between the rate of K- and Na-absorption by maize seedlings in water culture and the concentration of these elements in the solution was investigated in a series of experiments. Na-absorption appeared to be strongly inhibited by excess K, but only part of the K-absorption was inhibited by excess Na. These results are interpreted in terms of the carrier hypothesis. Two carriers are thought to be operative: 1. a highly selective carrier A transporting K only and hardly affected by excess Na (Michaelis-Menten constant = ab. 0.002 me/l); 2. a hardly selective carrier B transporting both K and Na (Michaelis-Menten constant = ab. 0.2 me/l).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01394750
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