ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
: The recovery of myofibrillar protein from beef heart using nonthermal acidic solubilization was evaluated. Postmortem time, 3 or 24 h, did not affect protein solubility (p 〉 0.05). Protein solubility and yield were detrimentally affected by salt addition. Recovery of extracted protein (yield) was maximized with 0 mM NaCl (60.8 ± 3.9%). SDS-PAGE confirmed myosin solubilization below pH 5 and suggested reduced protein solubility below pH 2.5. Extracted protein from beef heart was significantly lower in fat, cholesterol, ash, and total collagen. In addition, the protein profile using a 7% SDS-Page gel consisted mainly of heavy chain myosin (36%), 150 kDa proteins (15%), and actin (21%).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2002.tb09588.x
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