Publication Date:
1998-06-25
Description:
Signaling pathways that stabilize interleukin-2 (IL-2) messenger RNA (mRNA) in activated T cells were examined. IL-2 mRNA contains at least two cis elements that mediated its stabilization in response to different signals, including activation of c-Jun amino-terminal kinase (JNK). This response was mediated through a cis element encompassing the 5' untranslated region (UTR) and the beginning of the coding region. IL-2 transcripts lacking this 5' element no longer responded to JNK activation but were still responsive to other signals generated during T cell activation, which were probably sensed through the 3' UTR. Thus, multiple elements within IL-2 mRNA modulate its stability in a combinatorial manner, and the JNK pathway controls turnover as well as synthesis of IL-2 mRNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, C Y -- Del Gatto-Konczak, F -- Wu, Z -- Karin, M -- New York, N.Y. -- Science. 1998 Jun 19;280(5371):1945-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9632395" target="_blank"〉PubMed〈/a〉
Keywords:
Antigens, CD28/immunology
;
Antigens, CD3/immunology
;
Calcimycin/pharmacology
;
Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
;
Cyclosporine/pharmacology
;
Enzyme Activation
;
Enzyme Inhibitors/pharmacology
;
*Gene Expression Regulation
;
Humans
;
Imidazoles/pharmacology
;
Interleukin-2/*genetics
;
JNK Mitogen-Activated Protein Kinases
;
Jurkat Cells
;
Lymphocyte Activation
;
MAP Kinase Kinase 1
;
MAP Kinase Kinase 7
;
*Mitogen-Activated Protein Kinase Kinases
;
*Mitogen-Activated Protein Kinases
;
Protein Kinases/metabolism
;
Protein-Serine-Threonine Kinases/metabolism
;
Protein-Tyrosine Kinases/metabolism
;
Pyridines/pharmacology
;
RNA, Messenger/chemistry/genetics/*metabolism
;
Signal Transduction
;
T-Lymphocytes/immunology/*metabolism
;
Tetradecanoylphorbol Acetate/pharmacology
;
Transgenes
;
p38 Mitogen-Activated Protein Kinases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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