Publication Date:
2022-05-26
Description:
© The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Marine Micropaleontology 138 (2018): 90-104, doi:10.1016/j.marmicro.2017.10.002.
Description:
Transmission electron microscope (TEM) observation has revealed much about the
basic cell biology of foraminifera. Yet, there remains much we do not know about
foraminiferal cytology and physiology, especially for smaller benthic foraminifera, which
inhabit a wide range of habitats. Recently, some TEM-coupled approaches have been
developed to study correlative foraminiferal ecology and physiology in detail: Fluorescently
Labeled Embedded Core (FLEC)-TEM for observing foraminiferal life-position together with
their cytoplasmic ultrastructure, micro-X-ray computed tomography (CT)-TEM for observing
and reconstructing foraminiferal cytoplasm in three dimensions (3D), and
TEM-Nanometer-scale secondary ion mass spectrometry (NanoSIMS) for mapping of
elemental and isotopic compositions at sub-micrometer resolutions with known ultrastructure.
In this contribution, we review and illustrate these recent advances of TEM-coupled methods.
Description:
This work
was financially supported by the Grant-in-Aid for Scientific Research from the Ministry of
Education, Culture, Sports, Science and Technology, Japan (Scientific Research (C) grant
number 17K05697 to HN) and the Swiss National Science Foundation (grant no.
200021_149333). JMB’s contributions were funded by US NSF grants OCE-0551001 and
OCE-1634469, the WHOI Robert W. Morse Chair for Excellence in Oceanography, and The
Investment in Science Fund at WHOI. The micro-X-ray CT imaging was performed under the
cooperative research program of Center for Advanced Marine Core Research (CMCR), Kochi University (accept No. 17A021).
Keywords:
Ultrastructure
;
NanoSIMS
;
Micro-X-ray CT
;
Correlative microscopy
;
Isotope mapping
;
Microhabitat
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
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