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Sensitive fluorescence staining for RNA and DNA species separated in polyacrylamide gels (1982)

Neuhoff, Volker ; Huether, Gerald ; Engelhardt, Uta

Weinheim : Wiley-Blackwell

Electrophoresis 3 (1982), S. 172-174

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ISSN: 0173-0835

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: The fluorescent dye, Hoechst Dye No. 33258, was found to be of excellent quality for staining of not only DNA, as reported in the literature, but also of RNA species after separation in polyacrylamide micro gels. The staining procedure is simple and very sensitive. There are no problems with background staining and under certain conditions the nucleotide-dye complex is stable for a long time. The fluorescence intensity is linear to the amount of nucleic acid bound by the dye within certain limits.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/elps.1150030312

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