Publication Date:
2006-05-27
Description:
Yersinia species use a variety of type III effector proteins to target eukaryotic signaling systems. The effector YopJ inhibits mitogen-activated protein kinase (MAPK) and the nuclear factor kappaB (NFkappaB) signaling pathways used in innate immune response by preventing activation of the family of MAPK kinases (MAPKK). We show that YopJ acted as an acetyltransferase, using acetyl-coenzyme A (CoA) to modify the critical serine and threonine residues in the activation loop of MAPKK6 and thereby blocking phosphorylation. The acetylation on MAPKK6 directly competed with phosphorylation, preventing activation of the modified protein. This covalent modification may be used as a general regulatory mechanism in biological signaling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mukherjee, Sohini -- Keitany, Gladys -- Li, Yan -- Wang, Yong -- Ball, Haydn L -- Goldsmith, Elizabeth J -- Orth, Kim -- R01-AI056404/AI/NIAID NIH HHS/ -- R21-DK072134/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2006 May 26;312(5777):1211-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16728640" target="_blank"〉PubMed〈/a〉
Keywords:
Acetyl Coenzyme A/metabolism
;
Acetylation
;
Acetyltransferases/metabolism
;
Bacterial Proteins/*metabolism
;
Catalytic Domain
;
Cell Line
;
Cell-Free System
;
Electrophoresis, Polyacrylamide Gel
;
Enzyme Activation
;
Extracellular Signal-Regulated MAP Kinases/metabolism
;
Humans
;
I-kappa B Kinase/*metabolism
;
MAP Kinase Kinase 6/chemistry/*metabolism
;
MAP Kinase Signaling System
;
NF-kappa B/metabolism
;
Phosphorylation
;
Recombinant Proteins/metabolism
;
Serine/metabolism
;
Signal Transduction
;
Threonine/metabolism
;
Yersinia/*metabolism/pathogenicity
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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