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  • 1
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    Extension of cell life-span and telomere length in animals cloned from senescent somatic cells (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2000-04-28
    Description: The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (〈2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lanza, R P -- Cibelli, J B -- Blackwell, C -- Cristofalo, V J -- Francis, M K -- Baerlocher, G M -- Mak, J -- Schertzer, M -- Chavez, E A -- Sawyer, N -- Lansdorp, P M -- West, M D -- AG00378/AG/NIA NIH HHS/ -- AI29524/AI/NIAID NIH HHS/ -- GM56162/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Apr 28;288(5466):665-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA. rlanza@advancedcell.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10784448" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Blotting, Southern ; Cattle/*genetics ; *Cell Aging ; Cell Division ; Cells, Cultured ; Clone Cells ; *Cloning, Organism ; DNA, Complementary ; Embryo Transfer ; *Eye Proteins ; Female ; Fibroblasts ; Flow Cytometry ; In Situ Hybridization, Fluorescence ; Longevity ; Matched-Pair Analysis ; *Nerve Growth Factors ; *Nuclear Transfer Techniques ; Proteins/genetics ; RNA, Messenger/genetics/metabolism ; Serpins/genetics ; Telomere/*ultrastructure
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Identification of sister chromatids by DNA template strand sequences (2009)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2009-12-18
    Description: It is generally assumed that sister chromatids are genetically and functionally identical and that segregation to daughter cells is a random process. However, functional differences between sister chromatids regulate daughter cell fate in yeast and sister chromatid segregation is not random in Escherichia coli. Differentiated sister chromatids, coupled with non-random segregation, have been proposed to regulate cell fate during the development of multicellular organisms. This hypothesis has not been tested because molecular features to reliably distinguish between sister chromatids are not obvious. Here we show that parental 'Watson' and 'Crick' DNA template strands can be identified in sister chromatids of murine metaphase chromosomes using CO-FISH (chromosome orientation fluorescence in situ hybridization) with unidirectional probes specific for centromeric and telomeric repeats. All chromosomes were found to have a uniform orientation with the 5' end of the short arm on the same strand as T-rich major satellite repeats. The invariable orientation of repetitive DNA was used to differentially label sister chromatids and directly study mitotic segregation patterns in different cell types. Whereas sister chromatids appeared to be randomly distributed between daughter cells in cultured lung fibroblasts and embryonic stem cells, significant non-random sister chromatid segregation was observed in a subset of colon crypt epithelial cells, including cells outside positions reported for colon stem cells. Our results establish that DNA template sequences can be used to distinguish sister chromatids and follow their mitotic segregation in vivo.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757939/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757939/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Falconer, Ester -- Chavez, Elizabeth A -- Henderson, Alexander -- Poon, Steven S S -- McKinney, Steven -- Brown, Lindsay -- Huntsman, David G -- Lansdorp, Peter M -- R01 GM094146/GM/NIGMS NIH HHS/ -- RMF-92093/Canadian Institutes of Health Research/Canada -- England -- Nature. 2010 Jan 7;463(7277):93-7. doi: 10.1038/nature08644. Epub 2009 Dec 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Terry Fox Laboratory, B.C. Cancer Agency, Vancouver, British Columbia V5Z 1L3, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20016487" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Line ; Chromatids/*genetics/*metabolism ; Chromosome Segregation/*physiology ; Colon/cytology ; DNA, Satellite/metabolism ; Embryonic Stem Cells/cytology ; Epithelial Cells/cytology ; Fibroblasts/cytology ; Fluorescence ; In Situ Hybridization, Fluorescence/*methods ; Luminescent Measurements ; Lung/cytology ; Mice ; Mice, Inbred C57BL ; Mitosis ; Models, Biological ; Organ Specificity ; Substrate Specificity ; Telomere/metabolism ; Templates, Genetic
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
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  • 3
    Electronic Resource
    Electronic Resource
    A multispecies stock assessment of a pelagic coastal fishery of the south-west Gulf of Mexico (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 23 (1992), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Multispecies stock assessment based upon Schaefer's theory was applied to a coastal pelagic fishery (Spanish mackerel, Scomberomorus maculatus (Mitchill); king mackerel, S. cavalla (Cuvier) and the blue runner, Caranx fusus (Geoffroy-St Hilaire)), from the west central Gulf of Mexico. Linear and non-linear systems of equations were estimated by using a multiple stepwise regression technique. The values of interaction parameters show a clear competition between mackerels, and technological interdependences between the blue runner and mackerels, The maximum yield estimation was from 4000 to 5000 tonnes, obtained with 23 and 34 beach seines respectively, depending on the applied model. Two stages were observed from the statistical records; in the first the Spanish mackerel is the most important species while in the second the abundance of this species declines and the others remain at the same level. Significant interactions were found from the first data group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.1992.tb00600.x
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    Paper (German National Licenses)
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  • 4
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    Site Effects and Peak Ground Accelerations Observed in Guadalajara, Mexico, for the 9 October 1995 Mw 8 Colima-Jalisco, Earthquake (2014)
    Seismological Society of America (SSA)
    Details
    Publication Date: 2014-10-04
    Description: On 3 June 1932, an M s  8.2 shallow thrust subduction earthquake struck the Colima–Jalisco (CJ) region of Mexico at an epicentral distance of ~250 km from Guadalajara, the second largest town in Mexico. The return period of these CJ earthquakes has been estimated from 77 to 126 years, which suggests the next is likely to occur between 2009 and 2058. As a step toward estimating the seismic hazard posed by potential M s 8.2+ events on Guadalajara, we present a study consisting of the following: (1) the analysis of the strong ground motions recorded at Guadalajara on 11 free-field accelerographs for the CJ 9 October 1995 M w  8 earthquake; (2) the analysis of the site effects in Guadalajara observed for this earthquake; and (3) the estimation of the spatial distribution of peak ground acceleration (PGA) in Guadalajara for this event. We propose, validate, and apply a recurrent neural network (RNN) technique to the recorded PGA. Important site effects were identified (using the horizontal-to-vertical and H/H rocksite techniques) on Guadalajara’s sandy soil layers with thicknesses h 〉20 m to the basaltic rock basement. The estimated PGAs in Guadalajara for the CJ 1995 earthquake varied from ~2 to ~27 cm/s 2 for soil layers with h ≤5 m and h 〉50 m, respectively. We expect the hybrid technique to obtain broadband synthetics ( Chavez et al. , 2011 ) and the proposed RNN methodology can be used to estimate Guadalajara’s seismic hazard for M w 8+ CJ scenario earthquakes. Online Material: Figures of Fourier amplitude spectra, H/V and H/H spectral ratios, and signal-to-noise spectral ratios.
    Print ISSN: 0037-1106
    Electronic ISSN: 1943-3573
    Topics: Geosciences , Physics
    Published by Seismological Society of America (SSA)
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  • 5
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    Detection of G-quadruplex DNA in mammalian cells (2014)
    Oxford University Press
    Details
    Publication Date: 2014-01-28
    Description: It has been proposed that guanine-rich DNA forms four-stranded structures in vivo called G-quadruplexes or G4 DNA. G4 DNA has been implicated in several biological processes, but tools to study G4 DNA structures in cells are limited. Here we report the development of novel murine monoclonal antibodies specific for different G4 DNA structures. We show that one of these antibodies designated 1H6 exhibits strong nuclear staining in most human and murine cells. Staining intensity increased on treatment of cells with agents that stabilize G4 DNA and, strikingly, cells deficient in FANCJ, a G4 DNA-specific helicase, showed stronger nuclear staining than controls. Our data strongly support the existence of G4 DNA structures in mammalian cells and indicate that the abundance of such structures is increased in the absence of FANCJ. We conclude that monoclonal antibody 1H6 is a valuable tool for further studies on the role of G4 DNA in cell and molecular biology.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 6
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    Detection of G-quadruplex DNA in mammalian cells (2013)
    Oxford University Press
    Details
    Publication Date: 2013-10-24
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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