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  • 1
    Publication Date: 2019
    Description: 〈p〉Technologies that define the atomic-level structure of neutralization-sensitive epitopes on viral surface proteins are transforming vaccinology and guiding new vaccine development approaches. Previously, iterative rounds of protein engineering were performed to preserve the prefusion conformation of the respiratory syncytial virus (RSV) fusion (F) glycoprotein, resulting in a stabilized subunit vaccine candidate (DS-Cav1), which showed promising results in mice and macaques. Here, phase I human immunogenicity data reveal a more than 10-fold boost in neutralizing activity in serum from antibodies targeting prefusion-specific surfaces of RSV F. These findings represent a clinical proof of concept for structure-based vaccine design, suggest that development of a successful RSV vaccine will be feasible, and portend an era of precision vaccinology.〈/p〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 2015-11-04
    Description: We reconstructed the synaptic circuits of seven columns in the second neuropil or medulla behind the fly’s compound eye. These neurons embody some of the most stereotyped circuits in one of the most miniaturized of animal brains. The reconstructions allow us, for the first time to our knowledge, to study...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 3
    Publication Date: 1972-10-01
    Print ISSN: 0022-4928
    Electronic ISSN: 1520-0469
    Topics: Geography , Geosciences , Physics
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  • 4
    Publication Date: 2011-08-23
    Description: Simultaneous measurements with the millimeter-wave imaging radiometer (MIR), cloud lidar system (CLS), and the MODIS airborne simulator (MAS) were made aboard the NASA ER-2 aircraft over the western Pacific Ocean on 17-18 January 1993. These measurements were used to study the effects of clouds on water vapor profile retrievals based on millimeter-wave radiometer measurements. The CLS backscatter measurements (at 0.532 and 1.064 am) provided information on the heights and a detailed structure of cloud layers; the types of clouds could be positively identified. All 12 MAS channels (0.6-13 Am) essentially respond to all types of clouds, while the six MIR channels (89-220 GHz) show little sensitivity to cirrus clouds. The radiances from the 12-/Am and 0.875-gm channels of the MAS and the 89-GHz channel of the MIR were used to gauge the performance of the retrieval of water vapor profiles from the MIR observations under cloudy conditions. It was found that, for cirrus and absorptive (liquid) clouds, better than 80% of the retrieval was convergent when one of the three criteria was satisfied; that is, the radiance at 0.875 Am is less than 100 W/cm.sr, or the brightness at 12 Am is greater than 260 K, or brightness at 89 GHz is less than 270 K (equivalent to cloud liquid water of less than 0.04 g/cm). The range of these radiances for convergent retrieval increases markedly when the condition for convergent retrieval was somewhat relaxed. The algorithm of water vapor profiling from the MIR measurements could not perform adequately over the areas of storm-related clouds that scatter radiation at millimeter wavelengths.
    Keywords: Meteorology and Climatology
    Type: Laboratory for Hydrospheric Processes Research Publications; 165-166
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  • 5
    Publication Date: 2011-08-19
    Description: Data collected with the Advanced Microwave Moisture Sounder (AMMS), which operates in the 183.3 GHz range, are compared to measurements collected at 22 GHz in order to show that the 183 GHz measurements are more sensitive to total precipitable water (W) values than the 22 GHz measurements. Radiative transfer calculations for the upwelling microwave emission from the ocean surface were performed at the AMMS frequencies with a variety of atmospheric temperature and humidity profiles. The derived brightness temperatures at these frequencies are compared with W values derived from the humidity profiles. It is observed that the sensitivity between the brightness temperatures and W values at the AMMS channel is greater than 130 K/g per sq cm and 12 K/g per sq cm for the 22 GHz frequency.
    Keywords: METEOROLOGY AND CLIMATOLOGY
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  • 6
    Publication Date: 2013-08-29
    Description: Microwave radiometric measurements made from an aircraft altitude of 20 km at 183.3 + or - 2, 183.3 + or - 5, and 183.3 + or - GHz, and at 92 GHz were used to retrieve the atmospheric water vapor profiles. Retrieval techniques shown to function properly in cloudfree conditions, were improved and extended to profile water vapor under moderate cloud cover conditions. The retrieved water vapor profiles compare favorably with those of rawinsonde observations at times nearly concurrent with the radiometric measurements.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: ESA, Proceedings of the 1988 International Geoscience and Remote Sensing Symposium (IGARSS) '88 on Remote Sensing: Moving Towards the 21st Century, Volume 2; p 945-948
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  • 7
    Publication Date: 2013-08-29
    Description: This paper presents the first and the only simultaneous measurements of water vapor by MIR (Millimeter-wave Imaging Radiometer) and LASE (Lidar Atmospheric Sounding Experiment) on board the same ER-2 aircraft. Water vapor is one of the most important constituents in the Earth's atmosphere, as its spatial and temporal variations affect a wide spectrum of meteorological phenomena ranging from the formation of clouds to the development of severe storms. Its concentration, as measured in terms of relative humidity, determines the extinction coefficient of atmospheric aerosol particles and therefore visibility. These considerations point to the need for effective and frequent measurements of the atmospheric water vapor. The MIR and LASE instruments provide measurements of water vapor profiles with two markedly different techniques. LASE can give water vapor profiles with excellent vertical resolution under clear condition, while MIR can retrieve water vapor profiles with a crude vertical resolution even under a moderate cloud cover. Additionally, millimeter-wave measurements are relatively simple and provide better spatial coverage.
    Keywords: Geophysics
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  • 8
    Publication Date: 2011-08-23
    Description: Three subjects related to atmospheric water vapor profiling using the 183.3 GHz absorption line are discussed in this paper. First, data acquired by an airborne millimeter-wave imaging radiometer (MIR) over ocean surface in the western Pacific are used to estimate three-dimensional (3-D) distribution of atmospheric water vapor. The instrument's radiometric measurements with mixed vertical and horizontal polarizations require modifications to the retrieval algorithm used in the past. It is demonstrated that, after the modifications, the new algorithm can provide adequate retrieval of water vapor profiles, even though the measured data are of mixed polarizations. Next, the retrieved profiles, in terms of water vapor mixing ratio rho (g/kg), are compared with those measured in near concurrence by dropsondes from a research aircraft in the western Pacific and by a ground-based Raman lidar at Wallops Island, Virginia. The ratio of the standard deviation to the mean rho is found to be 0.12 at 0.25 km altitude and gradually degraded to 0.67 at the highest altitude of the retrieval of 10.25 km. Finally, the effect of the "initial guess" relative humidity profile on the final retrieved product is analyzed with respect to the condition for the convergent retrieval. It is found that the effect is minimal if the initial profile is not unrealistically different from the true one. If the initial profile is very different from the true one, the final retrieved product could be subject to a substantial error. Tightening of the convergent condition in the retrieval helped reduce magnitude of the error, but not remove it totally. It is concluded that an initial profile based on climatology is likely to provide most reliable retrieval results.
    Keywords: Meteorology and Climatology
    Type: Laboratory for Hydrospheric Processes Research Publications (ISSN 0196-2892); 163-164
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  • 9
    Publication Date: 2011-08-19
    Description: Upwelling radiometric measurements at 90 GHz and three side bands near 183 GHz are used to retrieve water vapor profiles over the ocean surface. An algorithm incorporating a new technique of handling moderate cloud cover is illustrated for the profiling of both relative humidity and water vapor burden. It is shown that the retrieved relative humidity profiles reflect gross features of the corresponding profiles recorded by the radiosondes. However, the retrieval generally cannot produce fine details of the observed profiles at altitudes where a rapid change in relative humidity occurs. For this reason, comparison of retrieved and observed values at a given altitude often yields an appreciable rms error. Profiling of water vapor burden, a parameter equivalent to total integrated water vapor above a certain altitude, results in much better agreement, as expected. The rms error obtained from the results of the retrieval at the surface is comparable to that derived from the combination of measurements at 18 GHz and 21 GHz channels of the Scanning Multichannel Microwave Radiometer aboard the Nimbus 7 satellite.
    Keywords: METEOROLOGY AND CLIMATOLOGY
    Type: Journal of Applied Meteorology (ISSN 0894-8763); 29; 1005-101
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  • 10
    Publication Date: 2011-08-19
    Description: This paper describes a method for the retrieval of total precipitable water (W) in dry atmospheres, which relies on the strong water vapor absorption line at 183 GHz (for W less than 0.6 g/sq cm) and on the absorption near 90 GHz (for W above 0.6 g/sq cm). The method is very sensitive to the variations of W less than 0.5 g/sq cm and is complementary to the established methods that use the weak 22 GHz water vapor absorption line to retrieve W in the 1-6 g/sq cm. The technique was demonstrated by the analysis of two Advanced Microwave Moisture Sounder observations of dry atmospheres following cold-air outbreaks on March 13, 1983, and February 23, 1986.
    Keywords: METEOROLOGY AND CLIMATOLOGY
    Type: Journal of Applied Meteorology (ISSN 0894-8763); 28; 146-154
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