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  • 1
    Electronic Resource
    Electronic Resource
    Enzyme-linked immunosorbent assay for didecyldimethylammonium chloride, a fungicide used by the forest products industry (1995)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 43 (1995), S. 1400-1406 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00053a050
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  • 2
    Electronic Resource
    Electronic Resource
    Species level identification of conifer associated Ceratocystis sapstain fungi by PCR-RFLP on a β-tubulin gene fragment (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 222 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The genus Ceratocystis includes several morphologically similar species commonly found as agents of sapstain in coniferous trees. In this paper we describe a simple and reliable polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique that aids in the identification and differentiation of these fungi. PCR was used to amplify a 1.3-kb fragment of the β-tubulin gene from C. coerulescens, C. pinicola, C. douglassi, C. resinifera, C. rufipenni, C. polonica and C. adiposa. The PCR amplicon from representative isolates was sequenced. This information was utilized to select restriction enzymes that generated species-specific RFLP patterns. This approach was tested on our collection of over 200 Ceratocystis isolates and identified the fungi with a high level of confidence, reducing the time needed to identify these species by classical methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00256-8
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  • 3
    Electronic Resource
    Electronic Resource
    Suppressive subtractive hybridization and differential screening identified genes differentially expressed in yeast and mycelial forms of Ophiostoma piceae (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 238 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ophiostoma piceae is a sap-staining fungus that colonizes and discolours wood. It has been established that the mycelium form but not the yeast form becomes pigmented in vitro. Suppressive subtractive hybridization PCR was used to isolate transcripts specifically upregulated in either the yeast or mycelial forms of O. piceae. Subtracted cDNAs were cloned and transformed into Escherichia coli. The yeast and mycelium specific clones were then screened by differential screening to reduce the possibility of isolating false positive clones and the differential expression of the two sets of cDNAs was confirmed by reverse Northern hybridization. Numerous genes appear to be specifically expressed in either the yeast or mycelial forms. Sequence analysis identified several cDNAs similar to known genes. However, a few cDNAs showed no similarity to sequences in the public databases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09753.x
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  • 4
    Electronic Resource
    Electronic Resource
    Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 237 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09682.x
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  • 5
    Electronic Resource
    Electronic Resource
    Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 222 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two synonymous sapstain species, Ophiostoma montium and Ophiostoma ips, which are vectored by Dendroctonus ponderosae and various bark beetles, respectively, were differentiated into separate species using growth and molecular characteristics. Analysis of 32 isolates of the two species from different countries showed that O. ips was able to grow at 35°C while O. montium was not. This growth-based differentiation was strongly supported by sequence data for the internal transcribed spacer (ITS), 5.8S and partial 28S rDNA, and the β-tubulin genes. The β-tubulin gene sequence data indicate that the two species can easily be differentiated with a single polymerase chain reaction (PCR) assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00304-5
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  • 6
    Electronic Resource
    Electronic Resource
    Phylogenetic relationship of Ophiostoma piliferum to other sapstain fungi based on the nuclear rRNA gene (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 195 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The nuclear rRNA gene of Ophiostoma piliferum was analyzed to understand its phylogenetic relationships to other sapstain fungi. Phylograms based on nucleotide sequences of the rRNA gene showed that the relationships between O. piliferum and other Ophiostoma species varied depending on the regions of the rRNA gene analyzed. Intraspecies variation in O. piliferum was found in the internal transcribed spacer regions, and the variation was related to the geographic origin of O. piliferum strains. A useful molecular marker for differentiating O. piliferum from other sapstain Ophiostoma species was generated by the HaeIII restriction fragment length polymorphism of the 26S rRNA gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10515.x
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  • 7
    Electronic Resource
    Electronic Resource
    A molecular approach for early monitoring of decay basidiomycetes in wood chips (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 211 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A DNA-based method for the early detection and identification of decay basidiomycetes on wood chips is described. From 26 species of wood-decaying basidiomycetes and 20 species of wood-inhabiting ascomycetes, DNA fragments containing the internal transcribed spacers, 5.8S and partial 28S rDNA were amplified by PCR using the ITS1-F–NL2 rDNA primer pair and analysed through sequencing and restriction digestion. A decay basidiomycete-specific restriction fragment length polymorphism (RFLP) pattern was generated by Dra I. Using this PCR-RFLP method the detection of decay fungi is possible 4 days after the inoculation of the wood chips with either a single culture or mixed species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11212.x
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  • 8
    Electronic Resource
    Electronic Resource
    Differentiation of sapstain fungi by restriction fragment length polymorphism patterns in nuclear small subunit ribosomal DNA (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 177 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A useful DNA fingerprinting method for wood staining fungi is described. The polymerase chain reaction (PCR) was used to amplify nuclear small subunit ribosomal DNA (SSrDNA) from 55 fungal isolates of 13 sapstain species belonging to the Aureobasidium, Ceratocystis, Leptographium, and Ophiostoma genera. To find polymorphisms useful in differentiating the isolates, the amplified SSrDNAs were digested with 10 selected restriction enzymes. Genus-specific restriction fragment length polymorphism (RFLP) patterns were determined by RsaI, StyI, and TaqI for the four genera. This PCR-RFLP analysis of SSrDNA offers an easy and rapid tool for differentiation of the major sapstain organisms on stained wood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb13726.x
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  • 9
    Electronic Resource
    Electronic Resource
    Endophytic colonization of spruce by plant growth-promoting rhizobacteria (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 29 (1999), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We evaluated internal tissue colonization of hybrid spruce (Picea glauca×P. engelmannii) seedlings by two plant growth-promoting rhizobacteria (PGPR) strains, Bacillus polymyxa strain Pw-2R and Pseudomonas fluorescens strain Sm3-RN, using surface sterilization-dilution plating and immunofluorescent antibody staining assays. Both strains were consistently detected inside spruce root and stem tissues 5 months after seed inoculation according to a surface sterilization-dilution plating assay. Internal tissue population sizes ranged from log 3.9 to log 5.0 cfu g−1 plant tissue. Visualization of bacteria using immunofluorescent antibody staining suggested that these microorganisms colonized root hair and cortical cells as well as stem vascular tissues. Our results confirm the capability of these two plant growth-promoting rhizobacterial strains to enter spruce root tissues after soil inoculation and ultimately colonize stem vascular tissue without causing visible symptoms of disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1999.tb00610.x
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  • 10
    Electronic Resource
    Electronic Resource
    Accumulation of an iodophilic polysaccharide during growth of Acetivibrio cellulolyticus on cellobiose (1981)
    Springer
    Archives of microbiology 129 (1981), S. 265-267 
    Details
    ISSN: 1432-072X
    Keywords: Acetivibrio cellulolyticus ; Growth on cellobiose ; Iodophilic polysaccharide accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maximum growth of Acetivibrio cellulolyticus in 1% cellobiose (w/v, added as filter sterilized solution) medium was observed after about 24h of incubation at 35°C. The metabolic end products of growth were H2, CO2, acetic acid, ethanol and glucose. Growth was adversely affected if cellobiose was autoclaved with the rest of the media ingredients. In the presence of an excess of cellobiose, the cells accumulated large quantities of an iodophilic polysaccharide (IPS). The maximum IPS accumulation (about 37% of the cell dry weight) was observed after about 12h growth under nitrogen-limiting conditions. Starvation of these cells anaerobically, in a pH 7.0 phosphate buffer for 10 h at 35°C, resulted in about 50% drop in the IPS. The results also indicated that A. cellulolyticus accumulated this iodophilic polysaccharide during growth on cellobiose but not during cultivation on cellulose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414695
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