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  • 1
    Electronic Resource
    Electronic Resource
    A Perchloric Acid–Naphthoquinone Method for the Histochemical Localization of Cholesterol (1961)
    [s.l.] : Nature Publishing Group
    Nature 192 (1961), S. 331-332 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE histochemical demonstration of cholesterol by adaptations1 of the Liebermann-Burchard reaction has never been satisfactory owing to diffusion of the coloured product and to destruction of the tissue by the concentrated sulphuric acid used in the method. The bismuth trichloride method2 for ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/192331b0
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  • 2
    Electronic Resource
    Electronic Resource
    Autoradiographic Demonstration of Cholesterol Filtration and Accumulation in Atheromatous Rabbit Aorta (1966)
    [s.l.] : Nature Publishing Group
    Nature 210 (1966), S. 175-176 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] PREVIOUS autoradiographic studies have indicated that the inner part of the rat aortic wall labels more rapidly than does the outer part after intravenous injection of tritium-labelled cholesterol1. This evidence was taken to show that cholesterol enters the aorta from the blood and flows from the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/210175a0
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  • 3
    Electronic Resource
    Electronic Resource
    Biological Sciences: Cellular Lipid Inclusions in the White Matter in Multiple Sclerosis (1971)
    [s.l.] : Nature Publishing Group
    Nature 233 (1971), S. 264-265 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Seventeen plaques from eight cases of multiple sclerosis and white matter from sixteen control patients, who had died from other neurological diseases, were fixed in routine saline-formol and transferred to 1% calcium acetate?10% formalin. Frozen sections were stained with haematoxylin-eosin, Sudan ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/233264a0
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  • 4
    Electronic Resource
    Electronic Resource
    Subcellular Preparation of Peripheral Nerve Myelin (1968)
    [s.l.] : Nature Publishing Group
    Nature 220 (1968), S. 171-173 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In discussion at a symposium at Liblice2, Professor E. Gutmann and Dr H. Koenig suggested that we should try to prepare a residue of myelin from dorsal spinal roots by plucking out their axons with forceps after treating these peripheral nerves with a glycine-diethylamine buffer3. This suggestion ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/220171a0
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  • 5
    Electronic Resource
    Electronic Resource
    Reappraisal of osmium tetroxide and OTAN histochemical reactions (1968)
    Springer
    Histochemistry and cell biology 16 (1968), S. 162-166 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Osmium tetroxide and OTAN histochemical reactions have been reappraised following recent publications in this journal by Ellbder and Lojda. Provided that the standard OTAN reaction is used on frozen sections of conventional thickness, unsaturated hydrophilic polar lipids are stained in an orange or red shade while unsaturated hydrophobic non-polar lipids are stained brown-black or black. We have confirmed the anomaly — pointed out by Elleder and Lojda — that the “polar lipid” reaction of the atherosclerotic plaque is essentially extinguised by acetone. Solubility and blockading methods provide circumstantial evidence that Elleder and Lojda's assumed OsO4-protein reactions are due to the lipid moiety of a lipoprotein complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280614
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  • 6
    Electronic Resource
    Electronic Resource
    Schiff reactions with lipids and the disputed terminal rinse with hydrochloric acid (1971)
    Springer
    Histochemistry and cell biology 28 (1971), S. 220-224 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have been concerned with the effect of 3N HCl on the reaction-products of lipids and Schiff's reagent in the PAS and plasmal reactions. Our conclusions are: 1. We agree with Elleder and Lojda (1971) that the intensity of the modified PAS and plasmal reactions is reduced if a terminal 3N HCl rinse is used. However, we consider that the term “rinse” is not synonomous with a 5–10 min wash or bath. 2. We do not agree that this reduction is substantial. After a 1 min rinse with 3N HCl, it amounts to only 9.1% with the plasmal and 25.4% with the modified PAS reaction. 3. We do not agree that this modest reduction is necessarily all due to the release of fatty (or glycollic) aldehydes from conjugation with Schiff's reagent; it could equally well be due to suppression of phospholipid (or other anion) staining by the residual basic fuchsin in Schiff's reagent. 4. We do not agree that the plasmal (or PAS) reaction of brain lipids on paper is nearly extinguished by a terminal rinse in 3N HCl: such a result is possibly due to imperfect washing of chromatograms. With a terminal rinse of 1 min, the reactions were reduced by 31.5% and nil, respectively. 5. We do not agree that “the influence of an acid mounting medium” is of any practical significance with these histochemical reactions. 6. Finally we wish to reiterate that the staining of phospholipids (or other anions) by the residual basic fuchsin in Schiff's reagent is potentially a very real hazard in the application of the modified PAS and plasmal methods. Our experiments suggest that this hazard can be obviated by the use of the brief terminal 3N HCl rinse. Even if the acid does remove part of the reaction product with some Schiff methods, it is perhaps safer on these occasions to lose some of the baby with the bath-water.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305676
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  • 7
    Electronic Resource
    Electronic Resource
    Osmium tetroxide as a histochemical and histological reagent (1967)
    Springer
    Histochemistry and cell biology 9 (1967), S. 68-77 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary From the evidence discussed it can be concluded that osmium tetroxide (OsO4) would be reduced to black OsO2 (or an equivalent compound) by the ethylene bonds of liquid or solid cis-unsaturated lipids or by the Δ 5-double bond in cholesterol in solid state in tissues. No evidence has been obtained to suggest that OsO4 is either reduced or bound by proteins and polysaccharides in tissue-sections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281808
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  • 8
    Electronic Resource
    Electronic Resource
    Comment on the note by Z. Lojda and M. Elleder (1971)
    Springer
    Histochemistry and cell biology 28 (1971), S. 229-230 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305678
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  • 9
    Electronic Resource
    Electronic Resource
    Degraded proteolipid in multiple sclerosis plaques-histochemical electrophoretic observations (1975)
    Springer
    Journal of molecular histology 7 (1975), S. 599-604 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis Electrophoretic analysis of multiple sclerosis plaques has disclosed the presence of a protein band with a mobility approximately the same as myelin proteolipid. The presence of this band was unexpected in view of the virtual absence of myelin from plaques. By application of histochemical staining procedures to electrophoretic ‘micro’ gels it has been shown that this plaque protein does not stain with Sudan Black (for lipid) and dimethylaminobenzaldehyde (for tryptophan) and cannot, therefore, be identified as normal proteolipid. The abnormal band is not associated with glial components or residual myelin within the plaque. We consider that this material might be a degradation product of myelin proteolipid formed during the demyelinating process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01003797
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  • 10
    Electronic Resource
    Electronic Resource
    The pH dependence of borohydride as an aldehyde reductant (1979)
    Springer
    Journal of molecular histology 11 (1979), S. 111-116 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis The aldehyde-reducing capacity of borohydride has been investigated in the sequence periodic acid-borohydride-periodic acid-Schiff and variants. Densitometric studies on rat colonic mucins show that borohydride incompletely blocks periodateengendered aldehydes unless the pH is above 8.2 Below this value, some aldehydes are not reduced and continue to be Schiff-stainable, while others are subsequently generated by the second exposure to periodic acid. The effect is more pronounced in paraffin than in cryostat sections, but does not apply to human colonic mucins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01041270
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