Publication Date:
1990-08-10
Description:
Expression of T cell receptor (TCR) V alpha genes in tumor-infiltrating lymphocytes (TILs) within intraocular melanoma was studied. Primers for 18 different human TCR V alpha families were used to analyze TCR V alpha-C alpha gene rearrangements in TIL in these melanomas obtained at surgery. A limited number of TCR V alpha genes were expressed and rearranged in these tumors, and TILs expressing V alpha 7 were found in seven of eight of these uveal melanomas. TCR gene usage is also restricted in experimental autoimmune disease, in T cells within organs like skin and other epithelial tissues, and in the brain of patients with multiple sclerosis (MS). The restricted usage of TCR genes in TIL may indicate that a specific antigen in these melanomas is targeted.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nitta, T -- Oksenberg, J R -- Rao, N A -- Steinman, L -- New York, N.Y. -- Science. 1990 Aug 10;249(4969):672-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics, Stanford University Medical Center, CA 94305.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2382141" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
DNA, Neoplasm/genetics
;
Humans
;
Lymphocytes/*immunology
;
Melanoma/genetics/*immunology
;
Molecular Sequence Data
;
Multigene Family
;
Oligonucleotide Probes
;
Polymerase Chain Reaction
;
RNA, Messenger/genetics
;
Receptors, Antigen, T-Cell/*genetics
;
Uveal Neoplasms/genetics/*immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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