Publication Date:
2003-02-01
Description:
Optical approaches for observing the dynamics of single molecules have required pico- to nanomolar concentrations of fluorophore in order to isolate individual molecules. However, many biologically relevant processes occur at micromolar ligand concentrations, necessitating a reduction in the conventional observation volume by three orders of magnitude. We show that arrays of zero-mode waveguides consisting of subwavelength holes in a metal film provide a simple and highly parallel means for studying single-molecule dynamics at micromolar concentrations with microsecond temporal resolution. We present observations of DNA polymerase activity as an example of the effectiveness of zero-mode waveguides for performing single-molecule experiments at high concentrations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Levene, M J -- Korlach, J -- Turner, S W -- Foquet, M -- Craighead, H G -- Webb, W W -- P41-RR04224/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2003 Jan 31;299(5607):682-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Applied and Engineering Physics, Graduate Program in Biochemistry, Molecular, and Cell Biology, Cornell University, Clark Hall, Ithaca, NY 14853, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12560545" target="_blank"〉PubMed〈/a〉
Keywords:
Biochemistry/instrumentation/*methods
;
Computer Simulation
;
Coumarins
;
DNA/biosynthesis
;
DNA-Directed DNA Polymerase/*metabolism
;
Deoxycytosine Nucleotides/*metabolism
;
Diffusion
;
Enzymes, Immobilized/metabolism
;
Finite Element Analysis
;
Fluorescence
;
Fluorescent Dyes
;
Ligands
;
Light
;
*Nanotechnology/instrumentation
;
Optics and Photonics
;
Spectrometry, Fluorescence/instrumentation/*methods
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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