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  • 1
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    Unknown
    Molecular identification of viral pathogens in SPF(Specific pathogen free) shrimp (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrim ; SPF ; Virus ; White Spot Syndrome Virus ; WSS ; Spawning ; Molecular
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
    Location Call Number Expected Availability
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    PAPER (OA)
  • 2
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    Unknown
    Development gene data bank of cultured shrimp species in Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
    Location Call Number Expected Availability
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    PAPER (OA)
  • 3
    facet.materialart.
    Unknown
    Molecular identification of viral pathogens in SPF (Specific pathogen free) shrimp (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25749 | 18721 | 2018-10-13 07:31:35 | 25749 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.
    Keywords: Biology ; Iran ; Shrim ; SPF ; Virus ; White Spot Syndrome Virus ; WSS ; Spawning ; Molecular
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 48
    Location Call Number Expected Availability
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    PAPER (OA)
  • 4
    facet.materialart.
    Unknown
    Development gene data bank of cultured shrimp species in Iran (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25750 | 18721 | 2018-10-13 07:33:30 | 25750 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Keywords: Biology ; Information Management ; Iran ; DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 72
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
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